Laboratory Information
| Name | AA View on WormBase |
|---|---|
| Allele designation | dh |
| Head | Antebi, Adam |
| Institution | Max Planck Institute, Cologne, Germany |
| Address | MPI for Biology of Ageing Group Antebi Joseph-Stelzmann-Str. 9b Cologne Germany |
| Website | http://www.age.mpg.de/index.php?id=vita_antebi |
| Gene classes | daf din dre gfat pges |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| AA1 | daf-12(rh257) X. | C. elegans | daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Occasional abnormal dauers under exhausted conditions. |
| AA10 | daf-12(rh286) X. | C. elegans | Weak heterochronic phenotypes in seam, intestine, somatic gonad. Class V allele. |
| AA120 | dhIs26. | C. elegans | dhIs26 [daf-12a::GFP + lin-15(+)]. DAF-12::GFP localized primarily in nucleus, except during mitosis. Expressed widely in most cells including tissues modified for dauer formation or by stage from embryo to adult, but most elevated and widespread during L2. |
| AA18 | daf-12(rh61rh412) X. | C. elegans | daf-d. Weak heterochronic phenotypes in seam, somatic gonad and intestine. Class III allele. |
| AA277 | lin-15B&lin-15A(n765) X; dhIs64. | C. elegans | dhIs64 [daf-9p::daf-9::GFP + lin-15(+)]. |
| AA278 | dhIs59. | C. elegans | dhIs59 [Topo::daf-9::GFP + lin-15(+)]. Perinuclear expression in a ventral pair of bilateral neurons identified as the IL1Vs or URAVs in the anterior ganglia. By mid-L2, expression in the cytoplasm of the hypodermis, the syncitial epidermis, but absent from midline, epidermal seam cells. Levels peak around the L2 molt and diminish during L4. In some cases, transient expression seen in the L3 vulval blast cells. Also expressed within the hermaphrodite spermatheca starting in late L4 larvae and continuing eve in old adults. In males, expression in IL1V/URAVs and hypodermis but not somatic gonad. In dauer larvae, strong expression in IL1V/URAV and specifically extends into axonal but not dendritic processes. In post-dauer stages, expression in a pattern similar to reproductively growing animals, except expression is absent in the hypodermis. Grow at 20C. May still contain lin-15(n765) mutation in the background. |
| AA292 | daf-36(k114) V. | C. elegans | Mig on low cholesterol. Single daf-c at 27C, weak Mig. Strong expression in intestine at all stages. Grow at 20C. |
| AA34 | daf-12(rh61) X. | C. elegans | daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. |
| AA408 | din-1(dh127) II. | C. elegans | daf-d, suppresses daf-12(rh61) daf-12(rh274) gonadal migration defects. |
| AA411 | din-1(dh149) II. | C. elegans | daf-d, suppresses daf-12(rh61) daf-12(rh274) gonadal migration defects. |
| AA426 | dre-1(dh99) V. | C. elegans | Precocious fusion of seam cells one stage earlier (prior to L3 molt); impenetrant gonadal migration defects; SynMig on daf-12 RNAi. |
| AA6 | daf-12(rh84) X. | C. elegans | daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. |
| AA699 | din-1(hd36) II. | C. elegans | non-Daf. Temperature-sensitive phenotypes: at 20C half of the animals are egg-laying defective with occasional mispositioned gonadal arms; at 25C, 18% arrest as embryos: those animals that hatch usually display variable morphology defects in body and pharynx; nearly all animals that live to adults are small, clear, slightly uncoordinated, constipated, and virtually sterile. Maintain at 20C or below. |
| AA776 | cyp-44A1(ok216) II. | C. elegans | |
| AA790 | lin-15B&lin-15A(n765) X; dhEx343. | C. elegans | dhEx343 [din-1p::din-1E::GFP + lin-15(+)]. Pick GFP+ to maintain. Animals with the array are GFP+ and non-Muv. Animals which have lost the array are Muv and non-GFP. din-1s::GFP is detected in hypodermis, seam, intestine, and somatic gonad including the distal tip cells. din-1s is also expressed in neurons, vulval precursors, body wall muscle, pharynx, and all tissues with heterochronic phenotypes or remodeled during dauer. Expression is first detected in a few nuclei by the comma stage of embryogenesis. By hatching, din-1s was widely expressed, albeit weakly. Overall expression in most tissues is detected at various levels into adult and in dauer larvae. Animals with the array are GFP+ and non-Muv. Animals which have lost the array are Muv and non-GFP. din-1p::din-1E::GFP was produced by cloning into Fire Lab vector L3781. |
| AA82 | daf-12(rh284) X. | C. elegans | Gonadal lead cell Mig. Weak heterochronic phenotype in intestine. Weakly daf-c at 25C. Class V allele. |
| AA83 | daf-12(rh62rh157) X. | C. elegans | daf-d. Strong heterochronic phenotypes in seam and intestine. Weak heterochronic phenotypes in somatic gonad. Class II allele. |
| AA85 | daf-12(rh285) X. | C. elegans | Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Weakly daf-c at 15C. Class IV allele. |
| AA86 | daf-12(rh61rh411) X. | C. elegans | Daf-d, weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| AA87 | daf-12(rh273) X. | C. elegans | Daf-c, gonadal Mig, weak heterochronic phenotypes in intestine and seam. Class VI allele. |
| AA88 | daf-12(rh193) X. | C. elegans | Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Heterochronic phenotypes less penetrant at 15C. Weakly daf-c at 25C. Class IV allele. |
| AA89 | daf-12(rh274) X. | C. elegans | daf-c. Gonadal Mig. Weak heterochronic phenotypes in intestine. Class VI allele. |
| AA968 | nhr-8(hd117) IV. | C.elegans | Mig on low cholesterol. Reference: Magner DB, et al. Cell Metab. 2013 Aug 6;18(2):212-24. doi: 10.1016/j.cmet.2013.07.007.PMID: 23931753 |
| DR1407 | daf-12(m583) X. | C. elegans | daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| DR427 | daf-12(m116) X. | C. elegans | daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| DR978 | daf-12(m419) X. | C. elegans | daf-d. Weak heterochronic phenotypes in seam, somatic gonad, and intestine. Class III allele. |
| DR979 | daf-12(m420) X. | C. elegans | daf-d. Weak heterochronic phenotypes in intestine. Class III allele. |
| DR980 | daf-12(m421) X. | C. elegans | daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| DR981 | daf-12(m422) X. | C. elegans | daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
| DR983 | daf-12(m424) X. | C. elegans | daf-d. Weak heterochronic phenotypes in intestine. Class III allele. |
| JT156 | daf-12(sa156) X. | C. elegans | daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| dh6 | Allele | substitution | nonsense |
| dh127 | Allele | substitution | nonsense |
| dh149 | Allele | deletion | |
| dh99 | Allele | substitution |