Laboratory Information

NameAA View on WormBase
Allele designationdh
HeadAntebi, Adam
InstitutionMax Planck Institute, Cologne, Germany
Address MPI for Biology of Ageing
Group Antebi
Joseph-Stelzmann-Str. 9b
Cologne
Germany
Website http://www.age.mpg.de/index.php?id=vita_antebi
Gene classes daf  din  dre  gfat  pges 

Strains contributed by this laboratory

Strain Genotype Species Description
AA1 daf-12(rh257) X. C. elegans daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Occasional abnormal dauers under exhausted conditions.
AA10 daf-12(rh286) X. C. elegans Weak heterochronic phenotypes in seam, intestine, somatic gonad. Class V allele.
AA120 dhIs26. C. elegans dhIs26 [daf-12a::GFP + lin-15(+)]. DAF-12::GFP localized primarily in nucleus, except during mitosis. Expressed widely in most cells including tissues modified for dauer formation or by stage from embryo to adult, but most elevated and widespread during L2.
AA18 daf-12(rh61rh412) X. C. elegans daf-d. Weak heterochronic phenotypes in seam, somatic gonad and intestine. Class III allele.
AA199 unc-29(e1072) I; daf-9(dh6)/+ X. C. elegans Heterozygous. Heterozygotes will segregate dauers and wild-type. Pick wild-type and check for correct segregation to maintain.
AA277 lin-15B&lin-15A(n765) X; dhIs64. C. elegans dhIs64 [daf-9p::daf-9::GFP + lin-15(+)].
AA278 dhIs59. C. elegans dhIs59 [Topo::daf-9::GFP + lin-15(+)]. Perinuclear expression in a ventral pair of bilateral neurons identified as the IL1Vs or URAVs in the anterior ganglia. By mid-L2, expression in the cytoplasm of the hypodermis, the syncitial epidermis, but absent from midline, epidermal seam cells. Levels peak around the L2 molt and diminish during L4. In some cases, transient expression seen in the L3 vulval blast cells. Also expressed within the hermaphrodite spermatheca starting in late L4 larvae and continuing eve in old adults. In males, expression in IL1V/URAVs and hypodermis but not somatic gonad. In dauer larvae, strong expression in IL1V/URAV and specifically extends into axonal but not dendritic processes. In post-dauer stages, expression in a pattern similar to reproductively growing animals, except expression is absent in the hypodermis. Grow at 20C. May still contain lin-15(n765) mutation in the background.
AA292 daf-36(k114) V. C. elegans Mig on low cholesterol. Single daf-c at 27C, weak Mig. Strong expression in intestine at all stages. Grow at 20C.
AA34 daf-12(rh61) X. C. elegans daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele.
AA408 din-1(dh127) II. C. elegans daf-d, suppresses daf-12(rh61) daf-12(rh274) gonadal migration defects.
AA411 din-1(dh149) II. C. elegans daf-d, suppresses daf-12(rh61) daf-12(rh274) gonadal migration defects.
AA426 dre-1(dh99) V. C. elegans Precocious fusion of seam cells one stage earlier (prior to L3 molt); impenetrant gonadal migration defects; SynMig on daf-12 RNAi.
AA6 daf-12(rh84) X. C. elegans daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele.
AA699 din-1(hd36) II. C. elegans non-Daf. Temperature-sensitive phenotypes: at 20C half of the animals are egg-laying defective with occasional mispositioned gonadal arms; at 25C, 18% arrest as embryos: those animals that hatch usually display variable morphology defects in body and pharynx; nearly all animals that live to adults are small, clear, slightly uncoordinated, constipated, and virtually sterile. Maintain at 20C or below.
AA776 cyp-44A1(ok216) II. C. elegans
AA790 lin-15B&lin-15A(n765) X; dhEx343. C. elegans dhEx343 [din-1p::din-1E::GFP + lin-15(+)]. din-1s::GFP is detected in hypodermis, seam, intestine, and somatic gonad including the distal tip cells. din-1s is also expressed in neurons, vulval precursors, body wall muscle, pharynx, and all tissues with heterochronic phenotypes or remodeled during dauer. Expression is first detected in a few nuclei by the comma stage of embryogenesis. By hatching, din-1s was widely expressed, albeit weakly. Overall expression in most tissues is detected at various levels into adult and in dauer larvae. Animals with the array are GFP+ and non-Muv. Animals which have lost the array are Muv and non-GFP. din-1p::din-1E::GFP was produced by cloning into Fire Lab vector L3781.
AA82 daf-12(rh284) X. C. elegans Gonadal lead cell Mig. Weak heterochronic phenotype in intestine. Weakly daf-c at 25C. Class V allele.
AA83 daf-12(rh62rh157) X. C. elegans daf-d. Strong heterochronic phenotypes in seam and intestine. Weak heterochronic phenotypes in somatic gonad. Class II allele.
AA85 daf-12(rh285) X. C. elegans Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Weakly daf-c at 15C. Class IV allele.
AA86 daf-12(rh61rh411) X. C. elegans Daf-d, weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
AA87 daf-12(rh273) X. C. elegans Daf-c, gonadal Mig, weak heterochronic phenotypes in intestine and seam. Class VI allele.
AA88 daf-12(rh193) X. C. elegans Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Heterochronic phenotypes less penetrant at 15C. Weakly daf-c at 25C. Class IV allele.
AA89 daf-12(rh274) X. C. elegans daf-c. Gonadal Mig. Weak heterochronic phenotypes in intestine. Class VI allele.
DR1407 daf-12(m583) X. C. elegans daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
DR427 daf-12(m116) X. C. elegans daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
DR978 daf-12(m419) X. C. elegans daf-d. Weak heterochronic phenotypes in seam, somatic gonad, and intestine. Class III allele.
DR979 daf-12(m420) X. C. elegans daf-d. Weak heterochronic phenotypes in intestine. Class III allele.
DR980 daf-12(m421) X. C. elegans daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
DR981 daf-12(m422) X. C. elegans daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
DR983 daf-12(m424) X. C. elegans daf-d. Weak heterochronic phenotypes in intestine. Class III allele.
JT156 daf-12(sa156) X. C. elegans daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
dh6 Allele substitution nonsense
dh127 Allele substitution nonsense
dh149 Allele deletion
dh99 Allele substitution