Maintain at 15C. Low penetrance protruding vulva and bursting at permissive temperatures. pash-1(ram33[G179R]) is a G754A substitution (relative to the start codon in T22A3.5A.1) that results in a G179R substitution in the PASH-1 WW domain. Reference: Knittel TL, et al. Nat Commun. 2025 Jul 1;16(1):5595. doi: 10.1038/s41467-025-60721-5. PMID: 40595566.
