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JAZ515 C. elegans nep-2(ok2846) II; jlgEx251. Show Description
jlgEx251 [myo-3p::nep-2(cDNA)::wrmScarlet + elt-2p::GFP::NLS]. Maintain by picking GFP+ (intestinal nuclear). Extrachromosomal array with a red translational reporter for NEP-2 in myo-3 expressing muscle cells, co-injected with a nuclear GFP intestinal marker, in nep-2(ok2846) mutant background. Reference: Lo J, et al. Curr Biol. 2024 Oct 21;34(20):4715-4728.e4. doi: 10.1016/j.cub.2024.09.059. PMID: 39395417.
RB2138 C. elegans nep-2(ok2846) II. Show Description
T05A8.4. Homozygous. Outer Left Sequence: TTTACCTGTTTTGCCGGTTC. Outer Right Sequence: TTCAAACTTCCCAGTCCACC. Inner Left Sequence: CTTTTCACCCTTCCTACCCC. Inner Right Sequence: TCCTACTTCCAGATGGCACC. Inner Primer PCR Length: 1323 bp. Deletion Size: 362 bp. Deletion left flank: AGCAAACGCGCCCCACTGAACTTCTTTCAC. Deletion right flank: GAATTCGTAGAAATCATCGCAGGGGTCGAC. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807