Caenorhabditis Genetics Center (CGC)

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Strain Species Genotype Add
MAD21 C. elegans unc-119(ed3) III; dqIs3; ddIs26. Show Description
dqIs3 [pie-1p::dyn1B::GFP + unc-119(+)]. ddIs26 [pie-1::mCherry::par-6 + unc-119(+)]. Grow at 24 degrees.
MAD3 C. elegans unc-119(ed3) III; dqIs3. Show Description
dqIs3 [pie-1p::dyn1B::GFP + unc-119(+)]. Grow at 25 degrees.
MAD36 C. elegans unc-119(ed3) III; dqIs44. Show Description
dqIs44 [pie-1::mCherry::moe + unc-119(+)]. Can be maintained at room temperature; shift to 25C for RNAi and imaging. Strain does not do well when kept at 25C for multiple generations. Reference: Shivas JM & Skop AR. Mol Biol Cell. 2012 May;23(10):1917-27.