| BN1047 |
C. elegans |
bqSi536 II; bqSi548 IV. Show Description
bqSi536 [hsp16.41p::FRT::mCherry::his-58::FRT::dam::baf-1 + unc-119(+)] II. bqSi548 [dpy-7p::FLP + unc-119(+)] IV. Single-copy Dam::baf-1 and dpy-7p::FLP MosSCI transgenes for identifying DNA contacts of BAF-1 in hypodermal nuclei by DamID. Might still carry unc-119(ed3) or unc-119(ed9) in background. Reference: Romero-Bueno R, et al. EMBO J. 43(22): 5718-5746. doi: 10.1038/s44318-024-00261-8. PMID: 39367234.
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| BN1050 |
C. elegans |
bqSi777 II; baf-1(bq19[G12T]) III; bqSi548 IV. Show Description
bqSi777 [hsp16.41p::FRT::mCherry::his-58::FRT::dam::baf-1(G12T) + unc-119(+)] II. bqSi548 [dpy-7p::FLP + unc-119(+)] IV. Single-copy Dam::baf-1(G12T) and dpy-7p::FLP MosSCI transgenes for identifying DNA contacts of BAF-1(G12T) in hypodermal nuclei in baf-1(G12T) mutant background by DamID. Reference: Romero-Bueno R, et al. EMBO J. 43(22): 5718-5746. doi: 10.1038/s44318-024-00261-8. PMID: 39367234.
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| BN1097 |
C. elegans |
bqSi1030 II; bqSi548 IV. Show Description
bqSi1030 [hsp16.41p::FRT::mCherry::his-58::FRT::vhhGFP4::zif-1 + unc-119(+)] II. bqSi548 [dpy-7p::FLP + unc-119(+)] IV. Can be used for conditional depletion of GFP-tagged proteins in the hypodermis via anti-GFP nanobody fusion to ZIF1 (mediated by recruited ZIF-1 but NOT requiring ZF1 tags). Mechanism features heat-shock inactive ZIF-1 with FRT sites flanking inactivating sequence and hypodermal-specific expression of Cre to remove inactivating sequences to free vhhGFP4::zif-1 GFP degradation. May carry unc-119(ed3) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632
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| BN1414 |
C. elegans |
bqSi1411 II; bqSi548 IV. Show Description
bqSi1411 [hsp16.41p::FRT::mCherry::his-58::FRT::dam::rpb-6 + unc-119(+)] II. bqSi548 [dpy-7p::FLP + unc-119(+)] IV. Single-copy Dam::rpb-6 and dpy-7p::FLP MosSCI transgenes for for transcriptome analysis in hypodermal nuclei using RNA polymerase DamID. Might still carry unc-119(ed3) or unc-119(ed9) in background. Reference: Fragoso-Luna A, et al. Genetics. 223(1). doi: 10.1093/genetics/iyac166. PMID: 36321973.
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| BN550 |
C. elegans |
bqSi294 II; bqSi548 IV. Show Description
bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi548 [dpy-7p::FLP + unc-119(+)] IV. Heat shock produces green nuclei in the hypodermal cell lineage and red nuclei elsewhere.
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| BN561 |
C. elegans |
bqSi447 II; bqSi548 IV. Show Description
bqSi447 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::dam + unc-119(+)] II. bqSi548 [dpy-7p::FLP + unc-119(+)] IV. Single-copy GFP::Dam and dpy-7p::FLP MosSCI transgenes for detection of protein-DNA contacts in hypodermal nuclei by DamID. Might still carry unc-119(ed3) or unc-119(ed9) in background. Reference: Fragoso-Luna A, et al. Genetics. 223(1). doi: 10.1093/genetics/iyac166. PMID: 36321973.
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