Laboratory Information
| Name | YY View on WormBase |
|---|---|
| Allele designation | gg |
| Head | Scott G Kennedy |
| Institution | Harvard Medical School |
| Address | 77 Avenue Louis Pasteur NRB Room 266 Boston 02115 United States |
| Gene classes | hrde nrde znfx |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| YY11 | dcr-1(mg375) III. | C. elegans | Enhanced RNAi. Sterile at 25 degrees. Referenced in Pavelec et al. Genetics (2009). |
| YY13 | rrf-3(mg373) II; oxls12. | C. elegans | oxls12 [unc-47p::GFP + lin-15(+)]. Enhanced RNAi. Sterile at 25 degrees. [NOTE: the genotype of YY13 as previously annotated only as rrf-3(mg373)] References: Pavelec DM, et al. Genetics. 2009 Dec;183(4):1283-95. PMID: 19797044. McIntire SL, et al. Nature. 1997 Oct 23;389(6653):870-6. PMID: 9349821. |
| YY1325 | wago-4(gg620[3xflag::gfp::wago-4]) II. | C. elegans | 3xflag::gfp inserted into endogenous wago-4 locus using CRISPR/Cas9 engineering. 3xFLAG::GFP::WAGO-4 is partially functional in this strain. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683. |
| YY1446 | znfx-1(gg634[HA::tagRFP::znfx-1]) II. | C. elegans | HA::tagRFP inserted into endogenous znfx-1 locus using CRISPR/Cas9 engineering. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683. |
| YY1492 | mut-16(cmp3[mut-16::gfp::flag + loxP] I; znfx-1(gg634[HA::tagRFP::znfx-1]) II; pgl-1(gg640[pgl-1::3xflag::mCardinal]) IV. | C. elegans | gfp::flag inserted into endogenous mut-16 locus, 3xflag::gfp inserted into endogenous znfx-1 locus, and 3xflag::tagRFP inserted into endogenous pgl-1 locus using CRISPR/Cas9 engineering. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683. |
| YY156 | nrde-2(gg95) II. | C. elegans | Reference: Guang S, et al. Nature. 2010 Jun 24;465(7301):1097-101. |
| YY158 | nrde-3(gg66) X. | C. elegans | Nuclear RNAi defective. Reference: Guang et al., Science 321(5888):537-41 (2009). |
| YY160 | nrde-1(gg88) III. | C. elegans | eri-1(mg366) has been crossed out of the background. Reference: Burkhart KB, et al. PLoS Genet. 2011 Aug;7(8):e1002249. |
| YY166 | ergo-1(gg98) V. | C. elegans | Enhanced RNAi. Isolated in lin-15B RNAi screen. Referenced in Pavelec et al. Genetics (2009). |
| YY168 | ergo-1(gg100) V. | C. elegans | Enhanced RNAi. Isolated in lin-15B RNAi screen. Referenced in Pavelec et al. Genetics (2009). |
| YY169 | ergo-1(gg102) V. | C. elegans | Enhanced RNAi. Isolated in lin-15B RNAi screen. Referenced in Pavelec et al. Genetics (2009). |
| YY178 | ggIs1. | C. elegans | ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)]. Unknown if unc-119 is still in background. Reference: GuangS, et al. Nature. 2010 Jun 24;465(7301):1097-101. |
| YY186 | nrde-2(gg91) II. | C. elegans | T to A substitution at position 129 and Y to stop at position 24 in exon 2. Reference: GuangS, et al. Nature. 2010 Jun 24;465(7301):1097-101. |
| YY216 | eri-9(gg106) III. | C. elegans | Enhanced RNAi. Isolated in lin-15B RNAi screen. Referenced in Pavelec et al. Genetics (2009). |
| YY238 | nrde-3(gg64) X. | C. elegans | Nuclear RNAi defective. Reference: Guang et al., Science 321(5888):537-41 (2009). |
| YY346 | nrde-2(gg91) II; ggIs28. | C. elegans | ggIs28 [nrde-3p::3xFlag::GFP::nrde-2 ORF + unc-119(+)]. Unknown if unc-119 is still in background. Reference: Burkhart KB, et al. PLoS Genet. 2011 Aug;7(8):e1002249. |
| YY453 | nrde-4(gg129) IV. | C. elegans | Reference: Burkhart KB, et al. PLoS Genet. 2011 Aug;7(8):e1002249. |
| YY470 | dcr-1(mg375) III. | C. elegans | Enhanced RNAi response. Sterile at 25 C. Outcrossed from YY11; wild-type for mut-16. Superficially wild-type. |
| YY538 | hrde-1(tm1200) III. | C. elegans | Maintain at 15C. Heritable RNAi defective, germline mortality (Mrt) at 25C. Reference: Buckley BA, et al. Nature. 2012 Sep 20;489(7416):447-51. |
| YY916 | znfx-1(gg544[3xflag::gfp::znfx-1]) II. | C. elegans | GFP tag inserted at the N-terminus of endogenous znfx-1 via CRISPR/Cas9. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683. |
| YY968 | znfx-1(gg544[3xflag::gfp::znfx-1]) II; pgl-1(gg547[pgl-1::3xflag::tagRFP]) IV. | C. elegans | 3xflag::gfp inserted into endogenous znfx-1 locus, and 3xflag::tagRFP inserted into endogenous pgl-1 locus using CRISPR/Cas9 engineering. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683. |
| YY996 | znfx-1(gg561) II. | C. elegans | Heritable RNAi defective, germline immortality. Reference: Wan G, et al. Nature. 2018 May;557(7707):679-683. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| gg95 | |||
| gg66 | Allele | substitution | |
| gg88 | Allele | ||
| gg98 | Allele | deletion | frameshift |
| gg100 | Allele | substitution | nonsense |
| gg102 | Allele | substitution | |
| gg91 | Allele | ||
| gg106 | Allele | substitution | nonsense |
| gg64 | Allele | ||
| gg129 | Allele | substitution |