Laboratory Information
| Name | WU View on WormBase |
|---|---|
| Allele designation | am |
| Head | Kerry Kornfeld |
| Institution | Washington University School of Medicine, St. Louis, MO |
| Address | 4523 Clayton Ave Campus Box 8103 Dev Bio Dept St. Louis 63110 United States |
| Website | http://molecool.wustl.edu/kornfeld_lab/index.html |
| Gene classes | cdf cgr haly natc hizr zipt |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| BS3347 | lin-45(dx84) IV/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygotes are Unc and segregate Unc heterozygotes, non-Unc Steriles (lin-45 homozygotes), larval lethals, and dead eggs. dx84 is a strong lin-45 raf allele: dx84 homozygotes from dx84/+ heterozygotes are 47% larval lethal and among the adults, 100% are Sterile and Vul. |
| BS5183 | lin-45(oz201) IV/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygotes are Unc and segregate Unc, non-Unc Steriles, larval lethals, and dead eggs. oz201 is a strong lin-45 raf allele: oz201 homozygotes from oz201/+ heterozygotes segregate 55% larval lethal and the remaining adults are all Sterile and Vul. |
| EJ521 | lin-45(dx19) IV/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygotes are Unc and segregate Unc, non-Unc Steriles, larval lethals and dead eggs. dx19 is a strong lin-45 raf allele: dx19 homozygotes from dx19/+ heterozygous parents are 13% larval lethal and among the adults, 100% are Sterile and Vul. |
| MT6399 | let-60(n1046) IV; cdf-1(n2527) X. | C. elegans | cdf-1(n2527) suppresses the let-60(n1046gf) Muv phenotype from 95% Muv to <5% Muv. Suppression is weakly semi-dominant. |
| MT6400 | let-60(n1046) IV; cgr-1(n2528) X. | C. elegans | Low penetrance Muv (<5%). Due to variable penetrance larval lethal phenotype at 15C, maintain at 20C. |
| MT7949 | lin-1(n1761) IV. | C. elegans | n1761 cuases a partially penetrant "rod-like" larval lethal phenotype (70% at 20C) and a partially penetrant vulvaless phenotype (30% at 20C). It is a splice site defect predicted to eliminate 62 amino acids at the C-terminus. |
| WU1036 | natc-1(am138) V. | C. elegans | natc-1(am138) mutant animals are resistant to excess dietary zinc, cadmium, nickel, and copper. natc-1(am138) animals are also resistant to excess heat and oxidative stress while displaying reduced lifespan. References: Warnhoff K, et al. PLoS Genet. 2014 Oct 16;10(10):e1004703. Bruinsma JJ, et al. Genetics. 2008 Jun;179(2):811-28. |
| WU1055 | che-3(am178) I. | C. elegans | Ethosuximide resistant. Temperature sensitive dye-filling defective: WT dye-filling at 20C, dye-filling defective at 27C. |
| WU125 | lin-1(n1790) IV. | C. elegans | n1790 causes a partially penetrant "rod-like" larval lethal phenotype (17% at 20C) and a partially penetrant vulvaless defect (50% at 20C). A Smg mutation enhances these phenotypes. The n1790 mutation is a nonsense change at codon 352. |
| WU144 | lin-45(n1924) unc-24(e138) IV. | C. elegans | Weak lin-45 raf allele: 5% larval lethal. Otherwise, essentially WT vulval induction and fertility. n1924 effectively suppresses the let-60(n1046gf) Muv phenotype. Unc. |
| WU1500 | hizr-1(am286) X. | C. elegans | High zinc transcriptional activation - deficient (Zad-d). Avoid high zinc concentrations. [NOTE: this strain was previously described as hizr-1(am285); the correct allele name hizr-1(am286).] Reference: Warnhoff K, et al. PLoS Biol. 2017 Jan 17;15(1):e2000094. |
| WU156 | lin-1(n2515) dpy-13(e184) IV. | C. elegans | n2515 suppresses the let-60(gf) Muv phenotype. Isolation of lin-1 causes no significant vulval defect; it is a missense mutation that changes Proline 384 to Leucine. dpy-13 is a linked marker. |
| WU1563 | hizr-1(am285) X. | C. elegans | Gain-of-function allele: modified ligand binding domain constitutively binds HZA element. High zinc transcriptional activation - constitutive (Zad-c). [NOTE: this strain was previously described as hizr-1(am286); the correct allele name hizr-1(am285).] Reference: Warnhoff K, et al. PLoS Biol. 2017 Jan 17;15(1):e2000094. |
| WU1756 | lst-1(am302[3xFLAG::lst-1]) I. | C. elegans | Non-Glp; remains fertile even with sygl-1(RNAi). Endogenous lst-1 locus tagged with a 3xFLAG N-terminally. Visible in cytoplasm of 5-6 cell diameters of distal germline in young adults. Reference: Kocsisova Z, et al. Development. 2019 Apr 23;146(8). |
| WU1770 | sygl-1(am307[3xFLAG::sygl-1]) I. | C. elegans | Non-Glp; remains fertile even with lst-1(RNAi). Endogenous sygl-1 locus tagged with a 3xFLAG N-terminally. Visible in cytoplasm of 10-12 cell diameters of distal germline in young adults. Reference: Kocsisova Z, et al. Development. 2019 Apr 23;146(8). |
| WU1854 | pcn-1(am315[3xFLAG]) IV/nT1 [qIs51] (IV;V). | C. elegans | Homozygous maternal effect lethal mutation balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP am315 homozygotes (maternal effect lethal). Homozygous nT1[qIs51] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. 3xFLAG epitope allows antibody detection of full-length PCN-1. Reference: Kocsisova Z, et al. BMC Dev Biol. 2018 May 30;18(1):12. |
| WU209 | cdf-1(n2527) X. | C. elegans | Severe loss of function/null. Weak vulvaless phenotype (subtle defect in vulval cell lineages). Nonsense change of codon 186 of the predicted ORF C15B12.7. See also WBPaper00005255. |
| WU321 | cgr-1(n2528) X. | C. elegans | Slow growing at 20C. About 50% larval lethality at 15C. Maintain at 20C. |
| WU48 | lin-45(n2018) dpy-20(e1282) IV. | C. elegans | Intermediate severity of lin-45 raf allele: at 20C, 76% larval lethal and 24% display an abnormal vulva (either Egl, no discernable vulva, or PVul). Medium Dpy. Received new stock 11/2002. |
| WU49 | lin-45(n2506) unc-24(e138) IV. | C. elegans | Intermediate strength lin-45 raf allele: 86% larval lethal, 93% of adults display an abnormal vulva (either Egl, no discernable vulva, or PVul), and 3% of adults are Sterile. Unc. |
| WU51 | lin-45(n2520) unc-24(e138) IV. | C. elegans | Weak severity of lin-45 raf allele: WT viability, vulval induction and fertility but effectively suppresses the let-60(n1046gf) Muv phenotype. Unc. |
| WU53 | lin-45(n1925) unc-24(e138) IV. | C. elegans | Weak lin-45 raf allele: essentially WT viability, vulval induction and fertility but effectively suppresses the let-60(n1046gf) Muv phenotype. Unc. |
| WU57 | lin-45(n2510) unc-24(e138)/unc-5(e53) dpy-20(e1282) IV. | C. elegans | Heterozygotes are non-Unc and segregate non-Unc, Sterile Unc, and Dpy Unc. n2510 is a strong lin-45 raf allele: 100% of homozygotes are Sterile and Vul (no discernable vulva) or larval lethal. |
| WU680 | cgr-1(am114) X. | C. elegans | Slow growing at 20C. 100% larval lethality at 15C. Maintain at 20C. |
| WU684 | let-60(n1046) IV; cgr-1(am114) X. | C. elegans | Low penetrance Muv (<1%). Slow growing at 20C. 75% larval lethality at 15C. Maintain at 20C. |
| WU968 | osm-3(am177) IV. | C. elegans | Ethosuximide resistant. Dye-filling defective. |
| WU982 | natc-1(am134) V. | C. elegans | natc-1(am134) mutant animals are resistant to excess dietary zinc. References: Warnhoff K, et al. PLoS Genet. 2014 Oct 16;10(10):e1004703. Bruinsma JJ, et al. Genetics. 2008 Jun;179(2):811-28. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| am138 | Allele | deletion | |
| am178 | Allele | substitution | |
| am114 | Allele | deletion | |
| am177 | Allele | substitution | |
| am132 | Allele | substitution | nonsense |
| am130 | Allele | substitution | |
| am134 | Allele | substitution | nonsense |