Laboratory Information

NameWS View on WormBase
Allele designationop
HeadHengartner, Michael
InstitutionUniversity of Zurich, Zurich, Switzerland
Address Institute of Molecular Life Sciences University of Zurich Winterthurerstrasse 190 8057 Zurich

Website http://www.imls.uzh.ch/research/hengartner.html
Gene classes asm  bir  dam  egs  galt  gla  iap  mkk  ptp  rnf  stp  mina  samt 

Strains contributed by this laboratory

Strain Genotype Species Description
NF87 ced-12(k149) I. C. elegans Cell corpses persist as unengulfed, refractile dics. DTC mismigrates. Sequenced: point mutation R38>STOP, probably a null.
WS1137 cpb-3(op234) I. C. elegans High physiological germ-line apoptosis. op234 was originally assigned to gla-1, but cloning revealed it to be a point mutation in cpb-3 causing a C520Y substitution at an invariant cysteine within the conserved C/H domain.
WS1433 hus-1(op241) I; unc-119(ed3) III; opIs34. C. elegans opIs34 [hus-1p::hus-1::GFP + unc-119(+)]. Low-copy integrated array of 1144 bp hus-1 promoter and genomic coding sequence fused to GFP. Nuclear expression of GFP in germ cells and embryos. Integrated GFP construct completely rescues DNA damage induced cell cycle arrest defect and partially rescues DNA damage induced apoptosis of hus-1(op241).
WS1609 unc-69(ok339)/qC1 [dpy-19(e1259) glp-1(q339) III. C. elegans Heterozygotes are WT and segregate WT, Dpy Steriles and arrested L1 coilers. Fails to complement unc-50.
WS1642 rpoa-2(op259) I. C. elegans Maintain at 15-20C. Sterile at 25C. Apoptosis variant. Small germ line.
WS1973 opIs56. C. elegans opIs56 [egl-1p::2xNLS::GFP]. opIs56 is a low copy integrant of 3 kb 5' sequence from egl-1 fused to 2xNLS GFP. unc-119(ed3) should be outcrossed. Low basal GFP expression in embryos, meiotic germ cells and several neurons. GFP expression can be induced by ionizing radiation in all germ cells. Best viewed with dissected gonads.
WS2072 opIs76 I; unc-119(ed3) III. C. elegans opIs76 [cyb-1p::cyb-1::YFP + unc-119(+)]. The fusion protein partially rescues a cyb-1 null allele, so it is at least partially functional. non-Unc strain.
WS2170 unc-119(ed3) III; opIs110 IV. C. elegans opIs110 [lim-7p::YFP::actin + unc-119(+)] IV. YFP::ACT-5 expressed in somatic sheath cells, marks pre-disc corpses.
WS2265 hus-1(op244) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are WT GFP+ and segregate non-glowing hus-1 homozygotes and very rare homozygous hT2 glowing animals, and dead eggs. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and gut promoter driving GFP in the intestine. hus-1(op244) mutants from homozygous parents show an incompletely penetrant maternal effect embryonic lethality. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
WS2277 hus-1(op241) I. C. elegans Maintain at 15 or 20C. Mrt at 25C. Defective for DNA damage induced germ cell death and cell cycle arrest.
WS2972 gla-3(op216) I. C. elegans Sterile or nearly sterile. Extra programmed cell death in germline. Maintain at 20 C. Reference: Kritikou et al. 2006 Genes Dev 20(16):2279-92.
WS2973 gla-3(op212) I. C. elegans Sterile or nearly sterile. Extra programmed cell death in germline. Maintain at 20 C. Reference: Kritikou et al. 2006 Genes Dev 20(16):2279-92.
WS2974 gla-3(ep312) I. C. elegans Sterile or nearly sterile. Extra programmed cell death in germline. Maintain at 20 C. Reference: Kritikou et al. 2006 Genes Dev 20(16):2279-92.
WS3403 opIs170. C. elegans opIs170 [sdn-1p::sdn-1::GFP::unc-54 3'UTR + lin-15(+)]. lin-15B(n765) might be present in background. GFP expression in neurons and hypodermis. Maintain under normal conditions. Reference: Rhiner C, et al. Development. 2005 Oct;132(20):4621-33.
WS3404 opIs171. C. elegans opIs171 [sdn-1p::sdn-1::GFP::unc-54 3'UTR + lin-15(+)]. lin-15B&lin-15A(n765) might be present in background. GFP expression in neurons and hypodermis. Maintain under normal conditions. Reference: Rhiner C, et al. Development. 2005 Oct;132(20):4621-33.
WS3455 chk-2(gk212) V. C. elegans
WS3530 mtm-1(op309) I. C. elegans
WS4274 unc-119(ed3) III; opIs206. C. elegans opIs206 [hif-1p::hif-1(genomic)::GFP::hif-1 3'UTR + unc-119(+)]. Weak GFP signal in early embryonic stages (2-cell, 4-cell, 8-cell, etc.). Reference: Sendoel A, et al. Nature. 2010 Jun 3;465(7298):577-83.
WS4543 opIs257. C. elegans opIs257 [rad-54p::rad-54::YFP::rad-54 3'UTR + unc-119(+)].
WS4581 unc-119(ed3) III; opIs263. C. elegans opIs263 [rpa-1p::rpa-1::YFP + unc-119(+)]. YFP expression in soma and germline.
WS4591 unc-130(op459) II; hse-5(tm472) III. C. elegans
WS4700 opIs295 II; unc-119(ed3) III. C. elegans opIs295 [cdc-42p::GFP::cdc-42(genomic)::cdc-42 3'UTR + unc-119(+)] II.
WS4918 opIs310. C. elegans opIs310 [ced-1p::YFP::act-5::let-858 3'UTR + unc-119(+)].
WS4920 ced-2(n1994) IV; opIs310. C. elegans opIs310 [ced-1p::YFP::act-5::let-858 3'UTR + unc-119(+)].
WS5018 cdc-42(gk388) opIs295 II. C. elegans opIs295 [cdc-42p::GFP::cdc-42(genomic)::cdc-42 3'UTR + unc-119(+)] II. opIs295 fully rescues cdc-42 animals.
WS5229 unc-119(ed3) III; fan-1(tm423) IV; opIs406. C. elegans opIs406 [fan-1p::fan-1::GFP::let-858 3'UTR + unc-119(+)]. opIs406 resuces tm423. Reference: Kratz K, et al. Cell. 2010 Jul 9;142(1):77-88.
WS6608 unc-119(ed3) III; opEx1556. C. elegans opEx1556 [tyr-3p::tyr-3::GFP::tyr-3 3' UTR + unc-119(+)]. Pick non-Unc GFP+ to maintain. This strain replaced WS4623 opIs271, which was apparently not an integrated line.
WS841 ptp-2(op194) unc-4(e120)/mIn1 [dpy-10(e128)] II; him-5(e1490) V. C. elegans Heterozygotes are WT and segregate WT, Uncs which are sterile (>10 offspring) and Dpys. Throws males of all classes. mIn1 pka mC6.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
op147 Transposon insertion insertion
op236 Allele substitution
op241 Allele substitution
op259 Allele substitution
op244 Allele deletion
op216 Allele substitution splice_site
op212 Allele substitution
op312 Allele
op309 Allele substitution
op459 Allele substitution
op194 Allele deletion