Laboratory Information

NameWM View on WormBase
Allele designationne
HeadCraig C Mello
InstitutionUniversity of Massachusetts, Worcester, MA
Address 368 Plantation St.
AS5.2047
Worcester 01605
United States
Website http://www.umassmed.edu/pmm/faculty/mello.cfm
Gene classes alg  apr  cbp  csr  dcr  drh  ergo  gex  hda  imp  pir  pos  rba  rde 
sago  src  wago  wdfy  nyn  gcna 

Strains contributed by this laboratory

Strain Genotype Species Description
temp_name250 unc-5(e53) IV; mom-2(ne874) V; lon-2(e678) X. WM Incorrect genotype
temp_name282 eri-5(tm1705) IV. Does not exhibit expected phenotype
WM100 cks-1(ne549) IV. C. elegans Maintain at 15C. Produces dead eggs at 25C.
WM101 unc-24(e138) oma-1(ne411) IV; lon-2(e678) X. C. elegans Lon. Maintain at 15C. Produces dead eggs at 25C.
WM102 oma-1(ne3800) IV. C. elegans Maintain at 15C. Produces dead eggs at 25C.
WM103 unc-5(e53) mbk-2(ne3442) IV. C. elegans Unc. Maintain at 15C. Produces dead eggs at 25C.
WM104 unc-101(sy216) gsk-3(nr2047)/hIn1 [unc-54(h1040)] I. C. elegans Heterozygotes are WT and segregate WT, paralyzed Unc, and coilers which give only dead eggs (low brood size).
WM107 pgl-1(bn101) IV; neIs5. C. elegans neIs5 [Y43B11::pgl-1::GFP + rol-6(su1006)]. neIs5 is an integrated complex array of yeast YAC/genomic DNA expressing PGL-1::GFP as a C-terminal, translational fusion. Animals expressing PGL-1::GFP in the germline segregate GFP+ and GFP- progeny. Maintain by picking GFP+ Rollers. Please be aware, the transgene is frequently silenced and picking GFP+ animals is the only way to maintain a reasonable frequency of GFP-expressing animals. Maintain at 25C.
WM118 rde-1(ne300) V; neIs9 X. C. elegans neIs9 [myo-3::HA::RDE-1 + rol-6(su1006)]. Transgene rescues muscle RNAi defect. Rollers.
WM119 sago-2(tm894) ppw-1(tm914) I; C06A1.4(tm887) wago-4(tm1019) II; M03D4.6(tm1144) IV; sago-1(tm1195) V; neIs10 X. C. elegans neIs10 [myo-3::GFP::sago-2 + rol-6(su1006)]. Transgene rescues muscle RNAi defect. Rollers.
WM120 sago-2(tm894) ppw-1(tm914) I; C06A1.4(tm887) wago-4(tm1019) II; M03D4.6(tm1144) IV; sago-1(tm1195) V; neIs11 X. C. elegans neIs11 [myp-3::GFP::sago-1 + rol-6(su1006)]. Transgene rescues muscle RNAi defect. Rollers.
WM126 sago-2(tm894) ppw-1(tm914) I; C06A1.4(tm887) wago-4(tm1019) II; M03D4.6(tm1144) IV; sago-1(tm1195) V. C. elegans Deficient in germline and somatic RNAi.
WM140 drh-3(tm1217) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); him-8(e1489) IV. C. elegans Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP Sterile homozygotes. qIs48 [myo-2::GFP + pes-10::GFP + ges-1::GFP]. Homozygous hT2 animals are inviable. May have him-8(e1489) in background, but unsure.
WM149 dpy-5(e61) unc-13(e51) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are WT with pharyngeal GFP signal, and segragate WT GFP+, arrested hT2 aneuploids, and non-GFP Dpy Unc homozygotes. Homozygous hT2[bli-4 let-? qIs48] are inviable.
WM153 C04F12.1(tm1637) I. C. elegans Somatic RNAi deficient.
WM154 sago-2(tm894) I. C. elegans Somatic RNAi deficient.
WM155 ppw-2(tm1120) I. C. elegans Germline RNAi deficient.
WM156 R04A9.2(tm1116) X. C. elegans
WM157 Y49F6A.1(tm1127) II. C. elegans
WM158 ergo-1(tm1860) V. C. elegans Enhancer of RNAi.
WM159 T23D8.7(tm1163) I. C. elegans
WM160 sago-1(tm1195) V. C. elegans Deficient in somatic RNAi.
WM161 prg-1(tm872) I. C. elegans Temperature sensitive. Sterile at 25C. Maintain at 20C or below.
WM162 prg-2(tm1094) IV. C. elegans
WM170 unc-4(e120) pir-1(tm1496)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUncs, and Unc-4 animals which arrest at the L4 stage. Rarely, a recombination will occur and unc-4 and pir-1 will become unlinked. Propagate the strain by picking single WT animals and checking for correct segregation of progeny. 6/2007: Daniel Chavez notes that tm1496 may also delete part of sec-5, which could be responsible for the developmental arrest of tm1496.
WM171 eri-5(tm1705) IV. C. elegans
WM172 eri-3(tm1361) II. C. elegans Temperature sensitive sterile. Will throw dead eggs at 25C. 292 bp deletion.
WM179 nmy-2(ne3409) I. C. elegans Isolated from Hawaiian strain CB4856. Temperature sensitive embryonic lethal. Cytokinesis failure and polarity defects at 25C. Maintain at 15C. RNAi sensitive.
WM180 nmy-2(ne1490) I. C. elegans Isolated from Hawaiian strain CB4856. Temperature sensitive embryonic lethal. Cytokinesis failure and polarity defects at 25C. Maintain at 15C. RNAi sensitive.
WM182 csr-1(tm892) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc. csr-1(tm892) homozygotes are non-Unc and Sterile.
WM186 avr-14(ad1302) I; ttTi5605 II; unc-119(ed3) III; avr-15(ad1051) glc-1(pk54) V; neEx15. C. elegans neEx15 [myo-2::RFP + myo-2::avr-15(+) + unc-119(+)]. MosSCI recipient strain. neEx15 rescues unc-119, making the worms healthier for injection. The array is counter-selectable. Reference: Shirayama M, et al. Cell. 2012 Jul 6;150(1):65-77.
WM191 MAGO12 mutant C. elegans MAGO12 mutant. Full genotype: sago-2(tm894) ppw-1(tm914) ppw-2(tm1120) wago-2(tm2686) wago-1(tm1414) I; wago-11(tm1127) wago-5(tm1113) wago-4(tm1019) II; hrde-1(tm1200) sago-1(tm1195) III; wago-10(tm1186) V; nrde-3(tm1116) X. RNAi resistant. Temperature sensitive. Maintain at 15 C. Reference: Gu, et al. 2009 Mol Cell 36:234-44.
WM192 unc-119(ed3) III; neIs21. C. elegans neIs21 [wago-1::3xFLAG + unc-119(+)]. Reference: Gu, et al. 2009 Mol Cell 36:234-44.
WM193 csr-1(tm892) IV; neIs20. C. elegans neIs20 [pie-1::3xFLAG::csr-1 + unc-119(+)]. Partially rescues sterility (60% dead embryos). Unknown if unc-119(ed3) is still present in the background. Reference: Claycomb J, et al. 2009 Cell 139:123-34.
WM194 csr-1(tm892) IV; neIs20. C. elegans neIs20 [pie-1::GFP::csr-1 + unc-119(+)]. Partially rescues sterility (60% dead embryos). Unknown if unc-119(ed3) is still present in the background. Reference: Claycomb J, et al. 2009 Cell 139:123-34.
WM205 unc-119(ed3) III; neIs22. C. elegans neIs22 [wago-1::GFP + unc-119(+)]. Maintain at 24 C to avoid silencing of GFP expression. Reference: Gu, et al. 2009 Mol Cell 36:234-44.
WM206 drh-3(ne4253) I. C. elegans Mutator. Dominant RNA-i deficient. Temperature sensitive sterile. Maintain at 15-20 C. ne4253 is a C-to-T transition at position 3896 of the genomic DNA with respect to the +1 position of the ATG creating a T834M missense mutation. Reference: Gu, et al. 2009 Mol Cell 36:234-44.
WM207 mut-7(ne4255) III. C. elegans Dominat RNAi deficient. Temperature-sensitive sterile. E439K mutation in conserved Mg2+ coordinating residue. Reference: Gu W, et al. Mol Cell. 2009 Oct 23;36(2):231-44.
WM214 avr-14(ad1302) I; csr-1(tm892)/nT1 [unc-?(n754) let-?] IV; avr-15(ad1051) glc-1(pk54))/nT1 V; axIs36 X. C. elegans axIs36 [pes-10::GFP]. Heterozygotes are Unc and sensitive to ivermectin. Segregates csr-1 homozygotes (sterile, non-Unc, resisitant to ivermectin), dead embryos, and Unc heterozygotes. Maintain by picking Uncs. Do not distribute this strain; other labs should request it directly from the CGC. Reference: Claycomb JM, et al. Cell. 2009 Oct 2;139(1):123-34.
WM215 avr-14(ad1302) ego-1(om97) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); avr-15(ad1051) glc-1(pk54)) V. C. elegans Heterozygotes are wild-type, GFP+ and sensitive to ivermectin. Segregates non-GFP ego-1 homozygotes (sterile, resisitant to ivermectin), arrested hT2 aneuploids, and wild-type GFP+ heterozygotes. Maintain by picking GFP+. Do not distribute this strain; other labs should request it directly from the CGC. Reference: Claycomb JM, et al. Cell. 2009 Oct 2;139(1):123-34.
WM216 avr-14(ad1302) drh-3(tm1217) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); avr-15(ad1051) glc-1(pk54)) V. C. elegans Heterozygotes are wild-type, GFP+ and sensitive to ivermectin. Segregates non-GFP drh-3 homozygotes (sterile, resisitant to ivermectin), arrested hT2 aneuploids, and wild-type GFP+ heterozygotes. Maintain by picking GFP+. Do not distribute this strain; other labs should request it directly from the CGC. Reference: Claycomb JM, et al. Cell. 2009 Oct 2;139(1):123-34.
WM230 drh-3(ne3197) I. C. elegans Mutator. Dominant RNAi-deficient. Temperature-sensitive sterile. Maintain at 15-20C. ne3197 is a G840D missense mutation. Reference: Gu W, et al. Mol Cell. 2009 Oct 23;36(2):231-44.
WM231 drh-3(ne4254) I. C. elegans Mutator. Dominant RNAi-deficient. Temperature-sensitive sterile. Maintain at 15-20C. ne4254 is a G802E missense mutation. Reference: Gu W, et al. Mol Cell. 2009 Oct 23;36(2):231-44.
WM242 neSi12 II; unc-119(ed3) III. C. elegans neSi12 [cdk-1::GFP + Cbr-unc-119(+)] II. Reference: Shirayama M, et al. Cell. 2012 Jul 6;150(1):65-77.
WM27 rde-1(ne219) V. C. elegans RNAi deficient.
WM274 prg-1(tm872) I; neSi14 II; unc-119(ed3) III. C. elegans neSi14 [FLAG::prg-1 + Cbr-unc-119(+)] II. MosSCI insertion into ttTi5605 (LG II). Reference: Lee HC, et al. Cell. 2012 Jul 6;150(1):78-87.
WM275 prg-1(tm872) I; neSi15 II; unc-119(ed3) III. C. elegans neSi15 [FLAG::prg-1(D583A) + Cbr-unc-119(+)] II. MosSCI insertion into ttTi5605 (LG II). Reference: Lee HC, et al. Cell. 2012 Jul 6;150(1):78-87.
WM28 unc-32(e189) III; rde-1(ne219) V. C. elegans RNAi deficient. Unc.
WM286 mut-2(ne3370) I. C. elegans RNAi deficient. Temperature-sensitive sterile. In-frame deletion. Reference: Chen CC, et al. Curr Biol. 2005 Feb 22;15(4):378-83.
WM29 rde-2(ne221) I. C. elegans RNAi deficient. Transposon high-hopper strain. Him. Weak sterility.
WM30 mut-2(ne298) I. C. elegans RNAi deficient. Transposon high-hopper strain. Him. 10% dead embryos probably due to nondisjunction.
WM300 alg-4(ok1041) III; alg-3(tm1155) IV. C. elegans Temperature-sensitive male sterility. Maintain at 20C. Reference: Conine CC, et al. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3588-93.
WM31 dpy-17(e164) vab-7(e1562) III. C. elegans Dpy and Vab (tail abnormalities).
WM43 gex-3(zu196) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Zygotic phenotype: 100% of gex-3 homozygotes become Egl although they all make a normal looking L3/L4 vulva. Embryonic phenotype: complete loss of morphogenesis - hypodermal cells fail to intercalate or migrate. Received new stock from Erik Lundquist 11/2003.
WM45 rde-1(ne300) V. C. elegans RNAi deficient. Reference: Tabara H, et al. Cell 1999 Oct 15:99(2):123-32.
WM49 rde-4(ne301) III. C. elegans RNAi deficient.
WM53 alg-2(ok304) II. C. elegans T07D3.7. Homozygous viable, contains an out of frame deletion removing nucleotides encoding amino acids 34-374. This strain cannot be distributed to for-profit companies. Do not distribute this strain; other labs should request it from the CGC. URL: http://www.celeganskoconsortium.omrf.org.
WM65 src-1(cj293) let-502(sb118)/hT1 I; +/hT1 V. C. elegans Heterozygotes are WT and segregate WT, Uncs that give dead embryos (src-1 homozygotes), dead eggs, and mid-larval lethals (hT1 homozygotes). src-1 is linked to an unknown Unc. [Feb 2005: Paul Mains has found a temperature-sensitive let-502 mutation (called sb118) linked to src-1 in this strain. Not sure if this is the Unc mutation mentioned here, or a third mutation on this chromosome.]
WM73 mbk-2(ne992) IV. C. elegans Defective in mitotic spindle orientation. Mis-segregation of germline proteins PGL-1 and PIE-1. Change of cell fate (extra-gut, no gut). Defective in chromosome segregation. Temperature sensitive; maintain at 15C; 100% lethal at 25C.
WM74 wrm-1(ne1982) III. C. elegans Temperature sensitive embryonic lethal. Maintain at 15C. Received new stock 3/20/2008 from Mello lab.
WM75 wrm-1(tm514) III; neIs2 IV. C. elegans neIs2 [wrm-1::GFP + rol-6(su1006)] IV. Rollers.
WM79 rol-6(n1270) II; neEx1. C. elegans Rollers. n1270 is phenotypically wild type. neEx1[LIT-1::GFP + rol-6(su1006)]. LIT-1::GFP has full length LIT-1 fused to GFP in a YAC. Pick Rollers to maintain.
WM92 mom-4(ne1539) I. C. elegans Temperature sensitive embryonic lethal. Maintain at 15C. Received new stock 3/20/2008 from Mello lab. Replacement stock received from Mello Lab 01/14/2010.
WM93 lit-1(ne1991) III. C. elegans Temperature sensitive embryonic lethal. Maintain at 15C.
WM98 sma-2(e502) ced-7(n1892) cdk-1(ne236)/qC1 [dpy-19(e1259) glp-1(q339) III. C. elegans Heterozygotes are WT and segregate WT, Smalls which produces dead eggs, and Dpy Steriles. Throws males.
WM99 cdk-1(ne2257) III. C. elegans Maintain at 15C. Produces dead eggs at 25C.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
ne299 Allele insertion frameshift
ne219 Allele substitution
ne1539 Allele substitution
ne12 Allele
ne874 Allele substitution
ne300 Allele substitution nonsense
ne549 Allele substitution
ne411 Allele substitution
ne3800 Allele substitution
ne3442 Allele substitution
ne3409 Allele substitution
ne1490 Allele
ne4253 Allele
ne4255 Allele
ne3197 Allele substitution
ne4254 Allele substitution
ne3370 Allele deletion
ne221 Allele substitution
ne298 Allele substitution
ne301 Allele
ne992 Allele substitution
ne1982 Allele substitution
ne1991 Allele substitution
ne236 Allele substitution
ne2257 Allele substitution