Laboratory Information
| Name | WM View on WormBase |
|---|---|
| Allele designation | ne |
| Head | Craig C Mello |
| Institution | University of Massachusetts, Worcester, MA |
| Address | 368 Plantation St. AS5.2047 Worcester 01605 United States |
| Website | http://www.umassmed.edu/pmm/faculty/mello.cfm |
| Gene classes | alg apr cbp csr dcr drh ergo gex hda imp pir pos rba rde sago src wago wdfy nyn gcna |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| WM100 | cks-1(ne549) IV. | C. elegans | Maintain at 15C. Produces dead eggs at 25C. |
| WM101 | unc-24(e138) oma-1(ne411) IV; lon-2(e678) X. | C. elegans | Lon. Maintain at 15C. Produces dead eggs at 25C. |
| WM102 | oma-1(ne3800) IV. | C. elegans | Maintain at 15C. Produces dead eggs at 25C. |
| WM103 | unc-5(e53) mbk-2(ne3442) IV. | C. elegans | Unc. Maintain at 15C. Produces dead eggs at 25C. |
| WM104 | unc-101(sy216) gsk-3(nr2047)/hIn1 [unc-54(h1040)] I. | C. elegans | Heterozygotes are WT and segregate WT, paralyzed Unc, and coilers which give only dead eggs (low brood size). |
| WM118 | rde-1(ne300) V; neIs9 X. | C. elegans | neIs9 [myo-3::HA::rde-1 + rol-6(su1006)] X. Transgene rescues muscle RNAi defect. Rollers. |
| WM119 | sago-2(tm894) ppw-1(tm914) I; C06A1.4(tm887) wago-4(tm1019) II; M03D4.6(tm1144) IV; sago-1(tm1195) V; neIs10 X. | C. elegans | neIs10 [myo-3::GFP::sago-2 + rol-6(su1006)]. Transgene rescues muscle RNAi defect. Rollers. |
| WM120 | sago-2(tm894) ppw-1(tm914) I; C06A1.4(tm887) wago-4(tm1019) II; M03D4.6(tm1144) IV; sago-1(tm1195) V; neIs11 X. | C. elegans | neIs11 [myp-3::GFP::sago-1 + rol-6(su1006)]. Transgene rescues muscle RNAi defect. Rollers. |
| WM126 | sago-2(tm894) ppw-1(tm914) I; C06A1.4(tm887) wago-4(tm1019) II; M03D4.6(tm1144) IV; sago-1(tm1195) V. | C. elegans | Deficient in germline and somatic RNAi. |
| WM140 | drh-3(tm1217) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); him-8(e1489) IV. | C. elegans | Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP Sterile homozygotes. qIs48 [myo-2::GFP + pes-10::GFP + ges-1::GFP]. Homozygous hT2 animals are inviable. May have him-8(e1489) in background, but unsure. |
| WM149 | dpy-5(e61) unc-13(e51) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Heterozygotes are WT with pharyngeal GFP signal, and segragate WT GFP+, arrested hT2 aneuploids, and non-GFP Dpy Unc homozygotes. Homozygous hT2[bli-4 let-? qIs48] are inviable. |
| WM153 | vsra-1(tm1637) I. | C. elegans | Somatic RNAi deficient. vsra-1 is also known as csr-2/C04F12.1. |
| WM154 | sago-2(tm894) I. | C. elegans | Somatic RNAi deficient. |
| WM155 | ppw-2(tm1120) I. | C. elegans | Germline RNAi deficient. |
| WM156 | R04A9.2(tm1116) X. | C. elegans | |
| WM157 | Y49F6A.1(tm1127) II. | C. elegans | |
| WM158 | ergo-1(tm1860) V. | C. elegans | Enhancer of RNAi. |
| WM159 | T23D8.7(tm1163) I. | C. elegans | |
| WM160 | sago-1(tm1195) V. | C. elegans | Deficient in somatic RNAi. |
| WM161 | prg-1(tm872) I. | C. elegans | Temperature sensitive. Sterile at 25C. Maintain at 20C or below. |
| WM162 | prg-2(tm1094) IV. | C. elegans | |
| WM170 | unc-4(e120) pir-1(tm1496)/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs, and Unc-4 animals which arrest at the L4 stage. Rarely, a recombination will occur and unc-4 and pir-1 will become unlinked. Propagate the strain by picking single WT animals and checking for correct segregation of progeny. 6/2007: Daniel Chavez notes that tm1496 may also delete part of sec-5, which could be responsible for the developmental arrest of tm1496. |
| WM172 | eri-3(tm1361) II. | C. elegans | Temperature sensitive sterile. Will throw dead eggs at 25C. 292 bp deletion. |
| WM179 | nmy-2(ne3409) I. | C. elegans | Isolated from Hawaiian strain CB4856. Temperature sensitive embryonic lethal. Cytokinesis failure and polarity defects at 25C. Maintain at 15C. RNAi sensitive. |
| WM180 | nmy-2(ne1490) I. | C. elegans | Isolated from Hawaiian strain CB4856. Temperature sensitive embryonic lethal. Cytokinesis failure and polarity defects at 25C. Maintain at 15C. RNAi sensitive. |
| WM182 | csr-1(tm892) IV/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygotes are Unc. csr-1(tm892) homozygotes are non-Unc and Sterile. |
| WM186 | avr-14(ad1302) I; ttTi5605 II; unc-119(ed3) III; avr-15(ad1051) glc-1(pk54) V; neEx15. | C. elegans | neEx15 [myo-2::RFP + myo-2::avr-15(+) + unc-119(+)]. MosSCI recipient strain. neEx15 rescues unc-119, making the worms healthier for injection. The array is counter-selectable. Reference: Shirayama M, et al. Cell. 2012 Jul 6;150(1):65-77. |
| WM191 | MAGO12 mutant | C. elegans | MAGO12 mutant. Full genotype: sago-2(tm894) ppw-1(tm914) ppw-2(tm1120) wago-2(tm2686) wago-1(tm1414) I; wago-11(tm1127) wago-5(tm1113) wago-4(tm1019) II; hrde-1(tm1200) sago-1(tm1195) III; wago-10(tm1186) V; nrde-3(tm1116) X. RNAi resistant. Temperature sensitive. Maintain at 15 C. Reference: Gu, et al. 2009 Mol Cell 36:234-44. |
| WM192 | unc-119(ed3) III; neIs21. | C. elegans | neIs21 [wago-1::3xFLAG + unc-119(+)]. Reference: Gu, et al. 2009 Mol Cell 36:234-44. |
| WM193 | csr-1(tm892) IV; neIs20. | C. elegans | neIs20 [pie-1::3xFLAG::csr-1 + unc-119(+)]. Partially rescues sterility (60% dead embryos). Unknown if unc-119(ed3) is still present in the background. Reference: Claycomb J, et al. 2009 Cell 139:123-34. |
| WM194 | csr-1(tm892) IV; neIs20. | C. elegans | neIs20 [pie-1::GFP::csr-1 + unc-119(+)]. Partially rescues sterility (60% dead embryos). Unknown if unc-119(ed3) is still present in the background. Reference: Claycomb J, et al. 2009 Cell 139:123-34. |
| WM205 | unc-119(ed3) III; neIs22. | C. elegans | neIs22 [wago-1::GFP + unc-119(+)]. Maintain at 24 C to avoid silencing of GFP expression. Reference: Gu, et al. 2009 Mol Cell 36:234-44. |
| WM206 | drh-3(ne4253) I. | C. elegans | Mutator. Dominant RNA-i deficient. Temperature sensitive sterile. Maintain at 15-20 C. ne4253 is a C-to-T transition at position 3896 of the genomic DNA with respect to the +1 position of the ATG creating a T834M missense mutation. Reference: Gu, et al. 2009 Mol Cell 36:234-44. |
| WM207 | mut-7(ne4255) III. | C. elegans | Dominant RNAi deficient. Temperature-sensitive sterile. E439K mutation in conserved Mg2+ coordinating residue. Reference: Gu W, et al. Mol Cell. 2009 Oct 23;36(2):231-44. |
| WM214 | avr-14(ad1302) I; csr-1(tm892)/nT1 [unc-?(n754) let-?] IV; avr-15(ad1051) glc-1(pk54))/nT1 V; axIs36 X. | C. elegans | axIs36 [pes-10::GFP]. Heterozygotes are Unc and sensitive to ivermectin. Segregates csr-1 homozygotes (sterile, non-Unc, resisitant to ivermectin), dead embryos, and Unc heterozygotes. Maintain by picking Uncs. Do not distribute this strain; other labs should request it directly from the CGC. Reference: Claycomb JM, et al. Cell. 2009 Oct 2;139(1):123-34. |
| WM215 | avr-14(ad1302) ego-1(om97) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); avr-15(ad1051) glc-1(pk54)) V. | C. elegans | Heterozygotes are wild-type, GFP+ and sensitive to ivermectin. Segregates non-GFP ego-1 homozygotes (sterile, resisitant to ivermectin), arrested hT2 aneuploids, and wild-type GFP+ heterozygotes. Maintain by picking GFP+. Do not distribute this strain; other labs should request it directly from the CGC. Reference: Claycomb JM, et al. Cell. 2009 Oct 2;139(1):123-34. |
| WM216 | avr-14(ad1302) drh-3(tm1217) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); avr-15(ad1051) glc-1(pk54)) V. | C. elegans | Heterozygotes are wild-type, GFP+ and sensitive to ivermectin. Segregates non-GFP drh-3 homozygotes (sterile, resisitant to ivermectin), arrested hT2 aneuploids, and wild-type GFP+ heterozygotes. Maintain by picking GFP+. Do not distribute this strain; other labs should request it directly from the CGC. Reference: Claycomb JM, et al. Cell. 2009 Oct 2;139(1):123-34. |
| WM230 | drh-3(ne3197) I. | C. elegans | Mutator. Dominant RNAi-deficient. Temperature-sensitive sterile. Maintain at 15-20C. ne3197 is a G840D missense mutation. Reference: Gu W, et al. Mol Cell. 2009 Oct 23;36(2):231-44. |
| WM231 | drh-3(ne4254) I. | C. elegans | Mutator. Dominant RNAi-deficient. Temperature-sensitive sterile. Maintain at 15-20C. ne4254 is a G802E missense mutation. Reference: Gu W, et al. Mol Cell. 2009 Oct 23;36(2):231-44. |
| WM242 | neSi12 II; unc-119(ed3) III. | C. elegans | neSi12 [cdk-1::GFP + Cbr-unc-119(+)] II. Reference: Shirayama M, et al. Cell. 2012 Jul 6;150(1):65-77. |
| WM27 | rde-1(ne219) V. | C. elegans | RNAi deficient. |
| WM274 | prg-1(tm872) I; neSi14 II; unc-119(ed3) III. | C. elegans | neSi14 [FLAG::prg-1 + Cbr-unc-119(+)] II. MosSCI insertion into ttTi5605 (LG II). Reference: Lee HC, et al. Cell. 2012 Jul 6;150(1):78-87. |
| WM275 | prg-1(tm872) I; neSi15 II; unc-119(ed3) III. | C. elegans | neSi15 [FLAG::prg-1(D583A) + Cbr-unc-119(+)] II. MosSCI insertion into ttTi5605 (LG II). Reference: Lee HC, et al. Cell. 2012 Jul 6;150(1):78-87. |
| WM28 | unc-32(e189) III; rde-1(ne219) V. | C. elegans | RNAi deficient. Unc. |
| WM286 | mut-2(ne3370) I. | C. elegans | RNAi deficient. Temperature-sensitive sterile. In-frame deletion. Reference: Chen CC, et al. Curr Biol. 2005 Feb 22;15(4):378-83. |
| WM29 | rde-2(ne221) I. | C. elegans | RNAi deficient. Transposon high-hopper strain. Him. Weak sterility. |
| WM30 | mut-2(ne298) I. | C. elegans | RNAi deficient. Transposon high-hopper strain. Him. 10% dead embryos probably due to nondisjunction. |
| WM300 | alg-4(ok1041) III; alg-3(tm1155) IV. | C. elegans | Temperature-sensitive male sterility. Maintain at 20C. Reference: Conine CC, et al. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3588-93. |
| WM31 | dpy-17(e164) vab-7(e1562) III. | C. elegans | Dpy and Vab (tail abnormalities). |
| WM43 | gex-3(zu196) IV/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Zygotic phenotype: 100% of gex-3 homozygotes become Egl although they all make a normal looking L3/L4 vulva. Embryonic phenotype: complete loss of morphogenesis - hypodermal cells fail to intercalate or migrate. Received new stock from Erik Lundquist 11/2003. |
| WM45 | rde-1(ne300) V. | C. elegans | RNAi deficient. Reference: Tabara H, et al. Cell 1999 Oct 15:99(2):123-32. |
| WM49 | rde-4(ne301) III. | C. elegans | RNAi deficient. |
| WM53 | alg-2(ok304) II. | C. elegans | T07D3.7. Homozygous viable, contains an out of frame deletion removing nucleotides encoding amino acids 34-374. This strain cannot be distributed to for-profit companies. Do not distribute this strain; other labs should request it from the CGC. URL: http://www.celeganskoconsortium.omrf.org. |
| WM65 | src-1(cj293) let-502(sb118)/hT1 I; +/hT1 V. | C. elegans | Heterozygotes are WT and segregate WT, Uncs that give dead embryos (src-1 homozygotes), dead eggs, and mid-larval lethals (hT1 homozygotes). src-1 is linked to an unknown Unc. [Feb 2005: Paul Mains has found a temperature-sensitive let-502 mutation (called sb118) linked to src-1 in this strain. Not sure if this is the Unc mutation mentioned here, or a third mutation on this chromosome.] |
| WM73 | mbk-2(ne992) IV. | C. elegans | Defective in mitotic spindle orientation. Mis-segregation of germline proteins PGL-1 and PIE-1. Change of cell fate (extra-gut, no gut). Defective in chromosome segregation. Temperature sensitive; maintain at 15C; 100% lethal at 25C. |
| WM74 | wrm-1(ne1982) III. | C. elegans | Temperature sensitive embryonic lethal. Maintain at 15C. Received new stock 3/20/2008 from Mello lab. |
| WM75 | wrm-1(tm514) III; neIs2 IV. | C. elegans | neIs2 [wrm-1::GFP + rol-6(su1006)] IV. Rollers. |
| WM79 | rol-6(n1270) II; neEx1. | C. elegans | Rollers. n1270 is phenotypically wild type. neEx1[LIT-1::GFP + rol-6(su1006)]. LIT-1::GFP has full length LIT-1 fused to GFP in a YAC. Pick Rollers to maintain. |
| WM92 | mom-4(ne1539) I. | C. elegans | Temperature sensitive embryonic lethal. Maintain at 15C. Received new stock 3/20/2008 from Mello lab. Replacement stock received from Mello Lab 01/14/2010. |
| WM93 | lit-1(ne1991) III. | C. elegans | Temperature sensitive embryonic lethal. Maintain at 15C. |
| WM98 | sma-2(e502) ced-7(n1892) cdk-1(ne236)/qC1 [dpy-19(e1259) glp-1(q339) III. | C. elegans | Heterozygotes are WT and segregate WT, Smalls which produces dead eggs, and Dpy Steriles. Throws males. |
| WM99 | cdk-1(ne2257) III. | C. elegans | Maintain at 15C. Produces dead eggs at 25C. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| ne299 | Allele | insertion | frameshift |
| ne219 | Allele | substitution | |
| ne1539 | Allele | substitution | |
| ne12 | Allele | ||
| ne874 | Allele | substitution | |
| ne300 | Allele | substitution | nonsense |
| ne549 | Allele | substitution | |
| ne411 | Allele | substitution | |
| ne3800 | Allele | substitution | |
| ne3442 | Allele | substitution | |
| ne3409 | Allele | substitution | |
| ne1490 | Allele | ||
| ne4253 | Allele | ||
| ne4255 | Allele | ||
| ne3197 | Allele | substitution | |
| ne4254 | Allele | substitution | |
| ne3370 | Allele | deletion | |
| ne221 | Allele | substitution | |
| ne298 | Allele | substitution | |
| ne301 | Allele | ||
| ne992 | Allele | substitution | |
| ne1982 | Allele | substitution | |
| ne1991 | Allele | substitution | |
| ne236 | Allele | substitution | |
| ne2257 | Allele | substitution |