CB1005 |
unc-59(e1005) I. |
C. elegans |
Poor backward movement. Protrusive vulva. Gonad extrusion. Egg-laying defects. Recessive. |
temp_name39 |
spd-2(oj29) I. |
|
too sterile to maintain, rec'd het from Kevin |
WH108 |
abc-1(oj2) V/nT1 [unc-?(n754) let-?] (IV;V). |
C. elegans |
Heterozygotes are Unc and segregate Uncs, dead eggs and abc-1 homozygotes. At 25C abc-1 homozygotes are sterile Unc animals. At 16C abc-1 homozygotes are fertile animals that produce all dead eggs. |
WH113 |
spd-3(oj35) IV. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces slow growing, sterile Unc animals. L4 shift-up produces Mel phenotype. |
WH12 |
spd-1(oj5) I. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces thin sterile uncoordinated and weakly fertile animals. L4 shift-up produces Mel phenotype. |
WH135 |
stu-12(oj21) IV. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces leaky Mel phenotype. |
WH136 |
sle-1(oj23) II. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces Mel phenotype. |
WH137 |
stu-13(oj24) II. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces Mel phenotype. |
WH138 |
stu-14(oj26) X. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile, slightly Unc animals. L4 shift-up produces Mel phenotype. |
WH140 |
stu-15(oj28) V. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C sterile Unc animals. L4 shift-up produces Mel phenotype. |
WH142 |
stu-16(oj30) II. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc animals. L4 shift-up produces leaky Mel phenotype. |
WH143 |
mett-10(oj32) III. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc animals. L4 shift-up produces leaky Mel phenotype. mett-10 was formerly known as stu-18. |
WH144 |
stu-19(oj33) IV. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile animals that are slightly Unc. L4 shift-up produces Mel phenotype. |
WH145 |
cyk-2(oj34) IV. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile Unc animals. L4 shift-up produces leaky Mel phenotype. |
WH16 |
stu-9(oj13) II. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile animals that are slightly Unc (omega backers). L4 shift-up produces leaky Mel phenotype. |
WH161 |
stu-17(oj31) V. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc animals. L4 shift-up produces leaky Mel phenotype. |
WH163 |
nDf29/unc-13(e1091) spd-2(oj29) I. |
C. elegans |
Heterozygotes are WT and segregate WT, dead eggs and Uncs. At 25C the Unc Spds are Sterile; at 16C the Unc Spds are fertile but produce mostly dead eggs. Unc Spd animals will exhibit a fully penetrant maternal-effect embryonic lethal phenotype if shifted to 25C at the L4 stage. unc-13 spd-2 homozygotes may be propagated at 16C but may become sick causing immense frustration! See also WBPaper00004200. |
WH202 |
unc-61(n3169) V. |
C. elegans |
Poor backward movement. Protrusive vulva. Gonad extrusion. Egg-laying defects. Recessive. |
WH216 |
sep-1(e2406) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
C. elegans |
Heterozygotes have myo-2::GFP [qIs48] strongly expressed in the pharynx and are viable at 25C. 100% of sep-1 homozygotes are strongly Sterile Unc at 25C (at 20C, 100% are Sterile but no so Unc). Up to 30% of the homozygotes are Sterile at 16C. hT2[qIs48] homozygotes are dead. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. |
WH32 |
stu-10(oj14) I. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile animals that are Unc (slow omega backers). L4 shift-up produces leaky Mel phenotype. |
WH327 |
unc-119(ed3) III; ojIs23. |
C. elegans |
ojIs23 [pie-1p::GFP::C34B2.10]. |
WH33 |
stu-11(oj18). |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces leaky Mel phenotype. Unmapped. |
WH346 |
unc-119(ed3) III; ojIs34. |
C. elegans |
ojIs34 [GFP::car-1 + unc-119(+)]. N'-tagged GFP::CAR-1 (Y18D10A.17) fusion. Labels P-granules and small cytoplasmic puncta in all cells. Bombardment with pNL1.6::GFP::unc-119 (pfj-1::pie-1 promoter driving GFP::Y18D10A.17 (N-terminal) with unc-119 rescuing fragment). |
WH377 |
car-1(tm1753) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
C. elegans |
Heterozygotes are WT and GFP+. car-1 homozygotes are have no GFP and produce embryos with cytokinesis defect (embryonic lethal). Some recombination. |
WH49 |
stu-8(oj1) X. |
C. elegans |
Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile Unc animals. L4 shift-up produces leaky Mel phenotype. |
WH498 |
unc-119(ed3) III; ojEx83. |
C. elegans |
ojEx83 [pie-1p::mCherry::chin-1a + unc-119(+)]. Maintain by picking wild-type. Check occasionally for maintained mCherry expression. |
WH515 |
chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III. |
C. elegans |
Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77. |
WH527 |
cgef-1(gk261) X; ojIs40. |
C. elegans |
ojIs40 [wsp-1(G-protein-Binding-Domain)::GFP + unc-119(+)]. Maintain under normal condition. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77. |
WH528 |
cgef-1(gk261) X; ojIs26. |
C. elegans |
ojIs26 [GFP::nmy-2 + unc-119(+)]. Maintain under normal conditions. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77. |
WH529 |
cgef-1(gk261) X; ddIs?. |
C. elegans |
ddIs? [pie-1p::GFP::par-6 + unc-119(+)]. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77. |
WH530 |
cgef-1(gk261) X; itIs153. |
C. elegans |
itIs153 [pie-1p::par-2::GFP + rol-6(su1006) + N2 genomic DNA]. Maintain at 24 degrees; sick at 25 C, GFP lost at 20C. itIs153 is an integrated derivitive of axEx1094. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77. |
WH532 |
chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III; ddIs?. |
C. elegans |
ddIs? [pie-1p::GFP::par-6 + unc-119(+)]. Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77. |
WH533 |
chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III; ddIs?. |
C. elegans |
ddIs? [pie-1p::mCherry::par-6 + unc-119(+)]. Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77. |
WH556 |
mrck-1(ok586) V/nT1 [qIs51] (IV;V). |
C. elegans |
Maintain under normal condition. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok586 homozygotes (Lvl). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77. |
WH558 |
chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III; ojIs40. |
C. elegans |
ojIs40 [wsp-1(G-protein-Binding-Domain)::GFP + unc-119(+)]. Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77. |