Laboratory Information

NameWH View on WormBase
Allele designationoj
HeadWhite, John
InstitutionUniversity of Wisconsin, Madison, WI
Address LOCI, Room 271 Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, Wisconsin 53706

Website http://www.engr.wisc.edu/bme/faculty/white_john.html
Gene classes abc  cgef  chin  cmd  dnc  eff  mrck  sep  sle  slo  spd  sun 

Strains contributed by this laboratory

Strain Genotype Species Description
CB1005 unc-59(e1005) I. C. elegans Poor backward movement. Protrusive vulva. Gonad extrusion. Egg-laying defects. Recessive.
temp_name39 spd-2(oj29) I. too sterile to maintain, rec'd het from Kevin
WH108 abc-1(oj2) V/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Uncs, dead eggs and abc-1 homozygotes. At 25C abc-1 homozygotes are sterile Unc animals. At 16C abc-1 homozygotes are fertile animals that produce all dead eggs.
WH113 spd-3(oj35) IV. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces slow growing, sterile Unc animals. L4 shift-up produces Mel phenotype.
WH12 spd-1(oj5) I. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces thin sterile uncoordinated and weakly fertile animals. L4 shift-up produces Mel phenotype.
WH135 stu-12(oj21) IV. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces leaky Mel phenotype.
WH136 sle-1(oj23) II. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces Mel phenotype.
WH137 stu-13(oj24) II. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces Mel phenotype.
WH138 stu-14(oj26) X. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile, slightly Unc animals. L4 shift-up produces Mel phenotype.
WH140 stu-15(oj28) V. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C sterile Unc animals. L4 shift-up produces Mel phenotype.
WH142 stu-16(oj30) II. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc animals. L4 shift-up produces leaky Mel phenotype.
WH143 mett-10(oj32) III. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc animals. L4 shift-up produces leaky Mel phenotype. mett-10 was formerly known as stu-18.
WH144 stu-19(oj33) IV. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile animals that are slightly Unc. L4 shift-up produces Mel phenotype.
WH145 cyk-2(oj34) IV. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile Unc animals. L4 shift-up produces leaky Mel phenotype.
WH16 stu-9(oj13) II. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile animals that are slightly Unc (omega backers). L4 shift-up produces leaky Mel phenotype.
WH161 stu-17(oj31) V. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc animals. L4 shift-up produces leaky Mel phenotype.
WH163 nDf29/unc-13(e1091) spd-2(oj29) I. C. elegans Heterozygotes are WT and segregate WT, dead eggs and Uncs. At 25C the Unc Spds are Sterile; at 16C the Unc Spds are fertile but produce mostly dead eggs. Unc Spd animals will exhibit a fully penetrant maternal-effect embryonic lethal phenotype if shifted to 25C at the L4 stage. unc-13 spd-2 homozygotes may be propagated at 16C but may become sick causing immense frustration! See also WBPaper00004200.
WH202 unc-61(n3169) V. C. elegans Poor backward movement. Protrusive vulva. Gonad extrusion. Egg-laying defects. Recessive.
WH216 sep-1(e2406) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes have myo-2::GFP [qIs48] strongly expressed in the pharynx and are viable at 25C. 100% of sep-1 homozygotes are strongly Sterile Unc at 25C (at 20C, 100% are Sterile but no so Unc). Up to 30% of the homozygotes are Sterile at 16C. hT2[qIs48] homozygotes are dead. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
WH32 stu-10(oj14) I. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile animals that are Unc (slow omega backers). L4 shift-up produces leaky Mel phenotype.
WH327 unc-119(ed3) III; ojIs23. C. elegans ojIs23 [pie-1p::GFP::C34B2.10].
WH33 stu-11(oj18). C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces incompletely penetrant sterile Unc phenotype. L4 shift-up produces leaky Mel phenotype. Unmapped.
WH346 unc-119(ed3) III; ojIs34. C. elegans ojIs34 [GFP::car-1 + unc-119(+)]. N'-tagged GFP::CAR-1 (Y18D10A.17) fusion. Labels P-granules and small cytoplasmic puncta in all cells. Bombardment with pNL1.6::GFP::unc-119 (pfj-1::pie-1 promoter driving GFP::Y18D10A.17 (N-terminal) with unc-119 rescuing fragment).
WH377 car-1(tm1753) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are WT and GFP+. car-1 homozygotes are have no GFP and produce embryos with cytokinesis defect (embryonic lethal). Some recombination.
WH49 stu-8(oj1) X. C. elegans Temperature sensitive - maintain at 16C. L1 shift-up to 25C produces sterile Unc animals. L4 shift-up produces leaky Mel phenotype.
WH498 unc-119(ed3) III; ojEx83. C. elegans ojEx83 [pie-1p::mCherry::chin-1a + unc-119(+)]. Maintain by picking wild-type. Check occasionally for maintained mCherry expression.
WH515 chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III. C. elegans Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
WH527 cgef-1(gk261) X; ojIs40. C. elegans ojIs40 [wsp-1(G-protein-Binding-Domain)::GFP + unc-119(+)]. Maintain under normal condition. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
WH528 cgef-1(gk261) X; ojIs26. C. elegans ojIs26 [GFP::nmy-2 + unc-119(+)]. Maintain under normal conditions. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
WH529 cgef-1(gk261) X; ddIs?. C. elegans ddIs? [pie-1p::GFP::par-6 + unc-119(+)]. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
WH530 cgef-1(gk261) X; itIs153. C. elegans itIs153 [pie-1p::par-2::GFP + rol-6(su1006) + N2 genomic DNA]. Maintain at 24 degrees; sick at 25 C, GFP lost at 20C. itIs153 is an integrated derivitive of axEx1094. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
WH532 chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III; ddIs?. C. elegans ddIs? [pie-1p::GFP::par-6 + unc-119(+)]. Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
WH533 chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III; ddIs?. C. elegans ddIs? [pie-1p::mCherry::par-6 + unc-119(+)]. Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
WH556 mrck-1(ok586) V/nT1 [qIs51] (IV;V). C. elegans Maintain under normal condition. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok586 homozygotes (Lvl). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.
WH558 chin-1(tm1909)/sC1 [dpy-1(s2170) let(gk597)] III; ojIs40. C. elegans ojIs40 [wsp-1(G-protein-Binding-Domain)::GFP + unc-119(+)]. Maintain under normal conditions. Heterozygotes are WT, and segregate WT, occasional Dpy (non-let recombinant sC1 homozygotes), and nearly sterile tm1909 homozygotes. Pick WT and check for correct segregation of progeny to maintain. Reference: Kumfer et al. (2010) Mol Bio Cell21(2):266-77.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
oj2 Allele
oj35 Allele substitution
oj5 Allele substitution
oj21 Allele
oj23 Allele
oj24 Allele
oj26 Allele
oj28 Allele
oj30 Allele
oj32 Allele substitution
oj33 Allele
oj34 Allele
oj13 Allele
oj31 Allele
oj29 Allele substitution
oj55 Allele substitution
oj14 Allele
oj18 Allele
oj1 Allele