Laboratory Information
| Name | TX View on WormBase |
|---|---|
| Allele designation | te |
| Head | Rueyling Lin |
| Institution | UTSW Medical Center, Dallas, TX |
| Address | Room NA5.300 Mailcode 9148 6000 Harry Hines Blvd Dallas 75390 United States |
| Website | http://www.utsouthwestern.edu/findfac/research/0,,31720,00.html |
| Gene classes | gsp nit oma sdz |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| TX1246 | unc-119(ed3) III; teIs113. | C. elegans | teIs113 [pie-1p::GFP::H2B::zif-13'UTR 771bp + unc-119(+)]. A 771 bp genomic sequence downstream of the zif-1 stop codon (starting immediately after the stop codon) was cloned downstream of pie-1 promoter-driven GFP::H2B in the germline expression vector pID3.01B. Superficially wild-type. Reference: Oldenbroek M, et al. Dev Biol. 2012 Mar 15;363(2):388-98. |
| TX1377 | unc-119(ed3) III; teIs127. | C. elegans | teIs127 [pie-1p::GFP::H2B::mom-2 3'UTR + unc-119(+)] teIs127 construct includes a 557 bp genomic sequence beginning 100 bp upstream of the mom-2 stop codon was cloned downstream of pie-1 promoter-driven GFP::H2B. Superficially wild-type. Reference: Oldenbroek M, et al. Development. 2013 Nov;140(22):4614-23. |
| TX174 | oma-1(zu405te33) IV. | C. elegans | Dominant suppressor of zu405. Putative oma-1 null. [NOTE (09/2016; D. Greenstein, unpublished results): The two molecular changes in te33 are different than reported by Detwiler et al. (2001), but nonetheless result in a strong loss of oma-1 function. Sequencing of the original isolate of te33 gave the same result (S. Robertson and R. Lin, unpublished results).] This strain carrying oma-1(zu405te33) contains the following mutations: zu405 [C8889984T; P240L] and te33 [C8889978A, S238stop & C8889863T, H200Y]. |
| TX183 | oma-1(zu405te33)/nT1 [unc-?(n754) let-?] IV; oma-2(te51)/nT1 V. | C. elegans | Heterozygotes are Unc and segregate Unc and non-Unc steriles (oma-1; oma-2 homozygotes). The Oma animal has an empty uterus and lots of oocytes in the gonad arms. Maintain by picking Uncs. zu405 is a gain-of-function mutation which results in temperature sensitive, embryonic lethality. Loss-of-function mutation in either oma-1 or oma-2 alone does not have a detectable phenotype. te33 is a dominant suppressor of the zu405 embryonic lethality. te51 is a mutation that, in the oma-1(zu405te33) background, gives an Oma (Ooctye Maturation defective) phenotype. [NOTE (09/2016; D. Greenstein, unpublished results): The two molecular changes in te33 are different than reported by Detwiler et al. (2001), but nonetheless result in a strong loss of oma-1 function. Sequencing of the original isolate of te33 gave the same result (S. Robertson and R. Lin, unpublished results).] This strain carrying oma-1(zu405te33) contains the following mutations: zu405 [C8889984T; P240L] and te33 [C8889978A, S238stop & C8889863T, H200Y]. |
| TX189 | unc-119(ed3) III; teIs1. | C. elegans | teIs1[(pRL475) oma-1p::oma-1::GFP + (pDPMM016) unc-119(+)]. |
| TX20 | oma-1(zu405) IV. | C. elegans | Maintain at 15C. Give about 50% dead embryos at 15C. Gives 100% dead embryos at 20C and 25C. |
| TX335 | unc-119(ed3) III; teIs2 IV. | C. elegans | teIs2 [pie-1p::GFP::pop-1(cDNA)::pie-1 3' UTR + unc-119(+)] IV. GFP signal is stable at 16-25C and shows pop-1 asymmetry. Superficially wild-type. Reference: Robertson SM, et al. Development. 2017 Feb 1;144(3):419-429. |
| TX431 | oma-2(te51) V. | C. elegans | |
| TX585 | unc-119(ed3) III; teIs18 V. | C. elegans | teIs18 [sdz-23p::GFP::H2B::pie-1 3'UTR + Cbr-unc-119(+)]. Superficially wild-type. Integrated array carrying sdz-23 (F58G4.4) promoter fusion with bright GFP expression in E cell. Reference: Shetty P, et al. Dev Biol. 2005 Sep 15;285(2):584-92. |
| TX691 | unc-119(ed3) III; teIs46. | C. elegans | teIs46 [pRL1417; end-1p::GFP::H2B + unc-119(+)]. Maintain under normal conditions. |
| TX773 | teIs65 II; unc-119(ed3) III; him-3(e1147) IV. | C. elegans | teIs65 [pie-1p::GFP::plk-1(PBD) + unc-119(+)] II. Maintain at 25 degrees and by picking the most brightly fluorescing animals to avoid silencing of the transgene. teIs65 contains GFP fused to the PLK-1 protein-binding domain. Derived from injection of pRL1216. Reference: Nishi Y, et al. Development. 2008 Feb;135(4):687-97. |
| TX796 | unc-119(ed3) III; him-3(e1147) IV; teEx321. | C. elegans | teEx321[pRL1636 (Pmed-1::GFP::sys-1) pDPmm016]. GFP signal is very weak and can't be see using a dissecting microscope. Very low transmission. Most signal is nuclear and the signal is stronger in the posterior sister, e.g., MSp stronger than MSa, Ep stronger than Ea. Some cortical signal was also detected. Some larvae lack anterior gut or have gaps. Maintain by picking non-Uncs. |
| TX895 | unc-119(ed3) III; him-3(e1147) IV; teIs84 X. | C. elegans | teIs84 [end-3p::GFP::H2B + unc-119(+)] X. Him. Superficially wild-type. Integrated E-lineage specific GFP reporter. Reference: Robertson SM, et al. PLoS One. 2014 Sep 2;9(9):e106309. |
| TX903 | teIs90 I; unc-119(ed3) III; him-3(e1147) IV. | C. elegans | teIs90 [(pRL1483) pie-1p::GFP::taf-4 + unc119(+)]. Sick at high temperatures. Maintain at 20C, but it will silence after some generations. |
| TX964 | unc-119(ed3) III; him-3(e1147) IV; teIs98. | C. elegans | teIs98 [(pRL1450) pie-1p::GFP::sys-1 + unc-119(+)]. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| te33 | Allele | substitution | nonsense |
| te51 | Allele | substitution | splice_site |