Laboratory Information

NameTX View on WormBase
Allele designationte
HeadRueyling Lin
InstitutionUTSW Medical Center, Dallas, TX
Address Room NA5.300
Mailcode 9148
6000 Harry Hines Blvd
Dallas 75390
United States
Website http://www.utsouthwestern.edu/findfac/research/0,,31720,00.html
Gene classes gsp  nit  oma  sdz 

Strains contributed by this laboratory

Strain Genotype Species Description
PMD1 fem-1(hc17) IV; dal-1(dt2300) C. elegans Temperature-sensitive. Maintain at 15C. Homozygous double mutant is sterile at 25C. Intestinal morphology defects. Reference: Wang C, et al. Nat Commun. 2017 Dec 22;8(1):2270.
TX1246 unc-119(ed3) III; teIs113. C. elegans teIs113 [pie-1p::GFP::H2B::zif-13'UTR 771bp + unc-119(+)]. A 771 bp genomic sequence downstream of the zif-1 stop codon (starting immediately after the stop codon) was cloned downstream of pie-1 promoter-driven GFP::H2B in the germline expression vector pID3.01B. Superficially wild-type. Reference: Oldenbroek M, et al. Dev Biol. 2012 Mar 15;363(2):388-98.
TX1377 unc-119(ed3) III; teIs127. C. elegans teIs127 [pie-1p::GFP::H2B::mom-2 3'UTR + unc-119(+)] teIs127 construct includes a 557 bp genomic sequence beginning 100 bp upstream of the mom-2 stop codon was cloned downstream of pie-1 promoter-driven GFP::H2B. Superficially wild-type. Reference: Oldenbroek M, et al. Development. 2013 Nov;140(22):4614-23.
TX174 oma-1(zu405te33) IV. C. elegans Dominant suppressor of zu405. Putative oma-1 null. [NOTE (09/2016; D. Greenstein, unpublished results): The two molecular changes in te33 are different than reported by Detwiler et al. (2001), but nonetheless result in a strong loss of oma-1 function. Sequencing of the original isolate of te33 gave the same result (S. Robertson and R. Lin, unpublished results).] This strain carrying oma-1(zu405te33) contains the following mutations: zu405 [C8889984T; P240L] and te33 [C8889978A, S238stop & C8889863T, H200Y].
TX183 oma-1(zu405te33)/nT1 [unc-?(n754) let-?] IV; oma-2(te51)/nT1 V. C. elegans Heterozygotes are Unc and segregate Unc and non-Unc steriles (oma-1; oma-2 homozygotes). The Oma animal has an empty uterus and lots of oocytes in the gonad arms. Maintain by picking Uncs. zu405 is a gain-of-function mutation which results in temperature sensitive, embryonic lethality. Loss-of-function mutation in either oma-1 or oma-2 alone does not have a detectable phenotype. te33 is a dominant suppressor of the zu405 embryonic lethality. te51 is a mutation that, in the oma-1(zu405te33) background, gives an Oma (Ooctye Maturation defective) phenotype. [NOTE (09/2016; D. Greenstein, unpublished results): The two molecular changes in te33 are different than reported by Detwiler et al. (2001), but nonetheless result in a strong loss of oma-1 function. Sequencing of the original isolate of te33 gave the same result (S. Robertson and R. Lin, unpublished results).] This strain carrying oma-1(zu405te33) contains the following mutations: zu405 [C8889984T; P240L] and te33 [C8889978A, S238stop & C8889863T, H200Y].
TX189 unc-119(ed3) III; teIs1. C. elegans teIs1[(pRL475) oma-1p::oma-1::GFP + (pDPMM016) unc-119(+)].
TX20 oma-1(zu405) IV. C. elegans Maintain at 15C. Give about 50% dead embryos at 15C. Gives 100% dead embryos at 20C and 25C.
TX335 unc-119(ed3) III; teIs2 IV. C. elegans teIs2 [pie-1p::GFP::pop-1(cDNA)::pie-1 3' UTR + unc-119(+)] IV. GFP signal is stable at 16-25C and shows pop-1 asymmetry. Superficially wild-type. Reference: Robertson SM, et al. Development. 2017 Feb 1;144(3):419-429.
TX431 oma-2(te51) V. C. elegans
TX585 unc-119(ed3) III; teIs18 V. C. elegans teIs18 [sdz-23p::GFP::H2B::pie-1 3'UTR + Cbr-unc-119(+)]. Superficially wild-type. Integrated array carrying sdz-23 (F58G4.4) promoter fusion with bright GFP expression in E cell. Reference: Shetty P, et al. Dev Biol. 2005 Sep 15;285(2):584-92.
TX691 unc-119(ed3) III; teIs46. C. elegans teIs46 [pRL1417; end-1p::GFP::H2B + unc-119(+)]. Maintain under normal conditions.
TX773 teIs65 II; unc-119(ed3) III; him-3(e1147) IV. C. elegans teIs65 [pie-1p::GFP::plk-1(PBD) + unc-119(+)] II. Maintain at 25 degrees and by picking the most brightly fluorescing animals to avoid silencing of the transgene. teIs65 contains GFP fused to the PLK-1 protein-binding domain. Derived from injection of pRL1216. Reference: Nishi Y, et al. Development. 2008 Feb;135(4):687-97.
TX787 unc-119(ed3) III; him-3(e1147) IV; teIs70. C. elegans teIs70 [pie-1p::GFP::plk-1 + unc-119(+)] II. Maintain at 25 degrees and by picking the most brightly fluorescing animals to avoid silencing of the transgene. teIs70 contains GFP fused to full-length PLK-1. Derived from injection of pRL766. Reference: Nishi Y, et al. Development. 2008 Feb;135(4):687-97.
TX796 unc-119(ed3) III; him-3(e1147) IV; teEx321. C. elegans teEx321[pRL1636 (Pmed-1::GFP::sys-1) pDPmm016]. GFP signal is very weak and can't be see using a dissecting microscope. Very low transmission. Most signal is nuclear and the signal is stronger in the posterior sister, e.g., MSp stronger than MSa, Ep stronger than Ea. Some cortical signal was also detected. Some larvae lack anterior gut or have gaps. Maintain by picking non-Uncs.
TX895 unc-119(ed3) III; him-3(e1147) IV; teIs84 X. C. elegans teIs84 [end-3p::GFP::H2B + unc-119(+)] X. Him. Superficially wild-type. Integrated E-lineage specific GFP reporter. Reference: Robertson SM, et al. PLoS One. 2014 Sep 2;9(9):e106309.
TX903 teIs90 I; unc-119(ed3) III; him-3(e1147) IV. C. elegans teIs90 [(pRL1483) pie-1p::GFP::taf-4 + unc119(+)]. Sick at high temperatures. Maintain at 20C, but it will silence after some generations.
TX964 unc-119(ed3) III; him-3(e1147) IV; teIs98. C. elegans teIs98 [(pRL1450) pie-1p::GFP::sys-1 + unc-119(+)].

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
te33 Allele substitution nonsense
te51 Allele substitution splice_site