Laboratory Information
| Name | TU View on WormBase |
|---|---|
| Allele designation | u |
| Head | Martin Chalfie |
| Institution | Columbia University, New York, NY |
| Address | Columbia University Biological Sciences/Fairchild Building 1212 Amsterdam Ave room1012/18 NY 10027 United States |
| Website | http://www.columbia.edu/cu/biology/faculty-data/martin-chalfie/faculty.html |
| Gene classes | alkb ben cas ctl deg des dro lis mec mtd poml rex tab ensh txdc |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| CB1256 | him-3(e1256) IV. | C. elegans | Throws 11% males. |
| CB3474 | ben-1(e1880) III. | C. elegans | Benomyl Resistant. Dominant at 25C--Recessive at 15C. |
| CB3475 | mcm-4(e1466)/szT1 [lon-2(e678)] I; +/szT1 X. | C. elegans | Heterozygotes are WT and segregate WT, Lon males and thin sterile Unc animals. Maintain by picking WT. |
| TU1366 | deg-1(u506) X. | C. elegans | Recessive gain-of-function. Codon 393 changed from alanine (GCT) to threonine (ACT). Deg and Tab at all temperatures. Lethal at 15C (embryos arrest at the two-fold stage, larvae survive). |
| TU151 | mec-8(u303) I. | C. elegans | Mechanosensory abnormal. |
| TU1747 | deg-3(u662) V. | C. elegans | Dominant mutant phenotypes: Unc, Mec, Tab, Deg (late embryogenesis to L4). u662 was derived from DnT1 by recombination between the dominant Unc mutation and the recessive Lethal mutation. The dominant Unc mutation is what has been retained in this strain. This Unc mutation has been called unc-?(n754) in DnT1 strains. It is likely that unc-?(n754) and deg-3(u662) are the same mutation. |
| TU218 | mec-8(u218) I. | C. elegans | Temperature sensitive. Mechanosensory abnormal at 25 C. Maintain at 15 C. Reference: Chalfie & Sulston (1981) Dev Biol 82:358. |
| TU228 | mec-18(u228) X. | C. elegans | Mechanosensory abnormal (partial). |
| TU2362 | vab-15(u781) X. | C. elegans | Variably abnormal. Severe developmental defects. Partially lethal (approx. 2/3 fail to survive). Adult hermaphrodites have variably enlarged and shortened tails and the body cuticle is twisted. Severe egg-laying defect; some animals have a protruding vulva. Tab. Unc. Lack AVM, PVM, and PLM. ALM often fail to migrate or migrate a shorter distance. |
| TU2461 | mig-21(u787) III. | C. elegans | Abnormal migration of AVM and PVM cells. |
| TU265 | mec-17(u265) IV. | C. elegans | Mechanosensory abnormal. Late onset--newly hatched larvae are touch sensitive, later larvae and adults are touch insensitive. |
| TU282 | lin-32(u282) X. | C. elegans | Touch insensitive in tail. |
| TU300 | mec-7(n434) X. | C. elegans | Dominant. Touch insensitive. |
| TU3135 | mec-8(u218) I; rde-1(ne219) V; uIs46. | C. elegans | uIs46 [rde-1p::mec-2(intron9)::rde-1) + ceh-22::GFP]. Temperature sensitive; maintain at 15 C. RNAi-sensitive at 15 C. Mechanosensory abnormal at 25 C. Uses the ninth intron of mec-2, whose splicing depends on mec-8, to produce temperature-sensitive expression and temperature-sensitive RNAi. Reference: Calixto et al. (2010) Nature Methods 7:407-11. |
| TU3310 | uIs59. | C. elegans | uIs59 [unc-119p::YFP]. Pan-neuronal YFP expression. Maintain 15-20C. Reference: Calixto A, et al. (2010) Nature Methods 7:554-9. |
| TU3311 | uIs60. | C. elegans | uIs60 [unc-119p::YFP + unc-119p::sid-1]. Hypersensitive neuronal RNAi by feeding. Superficially wild-type. YFP detectable in neurons. Maintain 15-20 degrees. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
| TU3335 | lin-15B(n744) X; uIs57. | C. elegans | uIs57 [unc-119p::YFP + unc-119p::sid-1 + mec-6p::mec-6]; appears to map to LG V. Hypersensitive neuronal RNAi by feeding. Superficially wild-type. YFP detectable in neurons. Maintain 15-20 degrees; sick at 25 C. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
| TU3403 | ccIs4251 I; sid-1(qt2) V; uIs71. | C. elegans | ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific feeding RNAi. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
| TU3568 | sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs71. | C. elegans | uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific RNAi by feeding. Him (~50% males). Maintain 15-20 degrees. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
| TU3595 | sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs72. | C. elegans | uIs72 [pCFJ90(myo-2p::mCherry) + unc-119p::sid-1 + mec-18p::mec-18::GFP]. Hypersensitive neuronal RNAi by feeding. GFP detectable in TRNs. Him (~50% males). Maintain 15-20 degrees. Reference: Chalfie (2010) Worm Breeders Gazette. |
| TU3596 | sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X. | C. elegans | Him. Enhanced RNAi background. Maintain under normal conditions. |
| TU38 | deg-1(u38) X. | C. elegans | Touch insensitive-at the tail. Degeneration of a small set of neurons. TAB Touch ABnormal). |
| TU55 | mec-14(u55) III. | C. elegans | Mechanosensory abnormal. |
| TU75 | mec-15(u75) II. | C. elegans | Mechanosensory abnormal. Temperature sensitive. |
| TU899 | stDp2(X;II)/+ II; uDf1 X. | C. elegans | Strain throws WT and dead eggs. |
| TU900 | +/szT1 [lon-2(e678)] I; uDf1/szT1 X. | C. elegans | Heterozygotes are WT and segregate WT, Lon males and dead eggs. Maintain by picking WT. [4/98: Lon males are sickly and Unc. uDf1 appears to still be present.] |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| u297 | Allele | ||
| u38 | Allele | ||
| u218 | Allele | substitution | |
| u74 | Allele | substitution | |
| u506 | Allele | ||
| u303 | Allele | substitution | nonsense |
| u314 | Allele | substitution | nonsense |
| u662 | Allele | substitution | |
| u695 | Allele | ||
| u228 | Allele | ||
| u781 | Allele | substitution | splice_site |
| u787 | Allele | substitution | |
| u253 | Allele | deletion | |
| u265 | Allele | substitution | |
| u282 | Allele | ||
| u55 | Allele | ||
| u75 | Allele | substitution | nonsense |