Laboratory Information

NameTU View on WormBase
Allele designationu
HeadMartin Chalfie
InstitutionColumbia University, New York, NY
Address Columbia University
Biological Sciences/Fairchild Building
1212 Amsterdam Ave room1012/18
NY 10027
United States
Website http://www.columbia.edu/cu/biology/faculty-data/martin-chalfie/faculty.html
Gene classes alkb  ben  cas  ctl  deg  des  dro  lis  mec  mtd  poml  rex  tab  ensh 
txdc 

Strains contributed by this laboratory

Strain Genotype Species Description
CB1256 him-3(e1256) IV. C. elegans Throws 11% males.
CB3474 ben-1(e1880) III. C. elegans Benomyl Resistant. Dominant at 25C--Recessive at 15C.
CB3475 mcm-4(e1466)/szT1 [lon-2(e678)] I; +/szT1 X. C. elegans Heterozygotes are WT and segregate WT, Lon males and thin sterile Unc animals. Maintain by picking WT.
temp_name57 ctl-1(u800) II. not correct Marty Chalfie 1/03
temp_name9 nDf6 III; mnDp37(III;f). CGC Deleted from CGC collection; does not carry nDf6.
TU1366 deg-1(u506) X. C. elegans Recessive gain-of-function. Codon 393 changed from alanine (GCT) to threonine (ACT). Deg and Tab at all temperatures. Lethal at 15C (embryos arrest at the two-fold stage, larvae survive).
TU151 mec-8(u303) I. C. elegans Mechanosensory abnormal.
TU1747 deg-3(u662) V. C. elegans Dominant mutant phenotypes: Unc, Mec, Tab, Deg (late embryogenesis to L4). u662 was derived from DnT1 by recombination between the dominant Unc mutation and the recessive Lethal mutation. The dominant Unc mutation is what has been retained in this strain. This Unc mutation has been called unc-?(n754) in DnT1 strains. It is likely that unc-?(n754) and deg-3(u662) are the same mutation.
TU218 mec-8(u218) I. C. elegans Temperature sensitive. Mechanosensory abnormal at 25 C. Maintain at 15 C. Reference: Chalfie & Sulston (1981) Dev Biol 82:358.
TU228 mec-18(u228) X. C. elegans Mechanosensory abnormal (partial).
TU2362 vab-15(u781) X. C. elegans Variably abnormal. Severe developmental defects. Partially lethal (approx. 2/3 fail to survive). Adult hermaphrodites have variably enlarged and shortened tails and the body cuticle is twisted. Severe egg-laying defect; some animals have a protruding vulva. Tab. Unc. Lack AVM, PVM, and PLM. ALM often fail to migrate or migrate a shorter distance.
TU2461 mig-21(u787) III. C. elegans Abnormal migration of AVM and PVM cells.
TU265 mec-17(u265) IV. C. elegans Mechanosensory abnormal. Late onset--newly hatched larvae are touch sensitive, later larvae and adults are touch insensitive.
TU282 lin-32(u282) X. C. elegans Touch insensitive in tail.
TU300 mec-7(n434) X. C. elegans Dominant. Touch insensitive.
TU3135 mec-8(u218) I; rde-1(ne219) V; uIs46. C. elegans uIs46 [rde-1p::mec-2(intron9)::rde-1) + ceh-22::GFP]. Temperature sensitive; maintain at 15 C. RNAi-sensitive at 15 C. Mechanosensory abnormal at 25 C. Uses the ninth intron of mec-2, whose splicing depends on mec-8, to produce temperature-sensitive expression and temperature-sensitive RNAi. Reference: Calixto et al. (2010) Nature Methods 7:407-11.
TU3310 uIs59. C. elegans uIs59 [unc-119p::YFP]. Pan-neuronal YFP expression. Maintain 15-20C. Reference: Calixto A, et al. (2010) Nature Methods 7:554-9.
TU3311 uIs60. C. elegans uIs60 [unc-119p::YFP + unc-119p::sid-1]. Hypersensitive neuronal RNAi by feeding. Superficially wild-type. YFP detectable in neurons. Maintain 15-20 degrees. Reference: Calixto et al. (2010) Nature Methods 7:554-9.
TU3335 lin-15B(n744) X; uIs57. C. elegans uIs57 [unc-119p::YFP + unc-119p::sid-1 + mec-6p::mec-6]; appears to map to LG V. Hypersensitive neuronal RNAi by feeding. Superficially wild-type. YFP detectable in neurons. Maintain 15-20 degrees; sick at 25 C. Reference: Calixto et al. (2010) Nature Methods 7:554-9.
TU3401 sid-1(pk3321) V; uIs69 V. C. elegans uIs69 [pCFJ90 (myo-2p::mCherry) + unc-119p::sid-1]. Hypersensitive neuronal RNAi by feeding. Superficially wild-type. Maintain 15-20 degrees. [NOTE: uIs69 is closely linked and maps to the right of dpy-11. (C. Loer 08/2011) See strains LC105 and LC108.] Reference: Calixto et al. (2010) Nature Methods 7:554-9.
TU3403 ccIs4251 I; sid-1(qt2) V; uIs71. C. elegans ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific feeding RNAi. Reference: Calixto et al. (2010) Nature Methods 7:554-9.
TU3568 sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs71. C. elegans uIs71 [(pCFJ90) myo-2p::mCherry + mec-18p::sid-1]. TRN-specific RNAi by feeding. Him (~50% males). Maintain 15-20 degrees. Reference: Calixto et al. (2010) Nature Methods 7:554-9.
TU3595 sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X; uIs72. C. elegans uIs72 [pCFJ90(myo-2p::mCherry) + unc-119p::sid-1 + mec-18p::mec-18::GFP]. Hypersensitive neuronal RNAi by feeding. GFP detectable in TRNs. Him (~50% males). Maintain 15-20 degrees. Reference: Chalfie (2010) Worm Breeders Gazette.
TU3596 sid-1(pk3321) him-5(e1490) V; lin-15B(n744) X. C. elegans Him. Enhanced RNAi background. Maintain under normal conditions.
TU38 deg-1(u38) X. C. elegans Touch insensitive-at the tail. Degeneration of a small set of neurons. TAB Touch ABnormal).
TU55 mec-14(u55) III. C. elegans Mechanosensory abnormal.
TU75 mec-15(u75) II. C. elegans Mechanosensory abnormal. Temperature sensitive.
TU899 stDp2(X;II)/+ II; uDf1 X. C. elegans Strain throws WT and dead eggs.
TU900 +/szT1 [lon-2(e678)] I; uDf1/szT1 X. C. elegans Heterozygotes are WT and segregate WT, Lon males and dead eggs. Maintain by picking WT. [4/98: Lon males are sickly and Unc. uDf1 appears to still be present.]

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
u297 Allele
u38 Allele
u218 Allele substitution
u74 Allele substitution
u506 Allele
u303 Allele substitution nonsense
u314 Allele substitution nonsense
u662 Allele substitution
u695 Allele
u228 Allele
u781 Allele substitution splice_site
u787 Allele substitution
u253 Allele deletion
u265 Allele substitution
u282 Allele
u55 Allele
u75 Allele substitution nonsense