Laboratory Information

NameTB View on WormBase
Allele designationch
HeadBurglin, Tom
InstitutionKarolinksa Institute, Huddinge, Sweden
Address Dept. of Biosciences at Novum and Center for Genomics and Bioinformatics Karolinska Institutet Alfred Nobels Alle 7 Sodertorns Hogskola, Huddinge Huddinge 141 89

Gene classes ceh  cfim  glrx  grd  grl  hhat  hog  irx  ldb  ocam  qua  rbd  rnt  ttbk 

Strains contributed by this laboratory

Strain Genotype Species Description
TB1 ceh-6(mg60)/dpy-5(e61) unc-29(e1072) I. C. elegans ceh-6(mg60) is lethal. Maintain by picking WT and check that it throws 1/4 Dpys. mg60 is a 1.3 kb deletion that removes part of the conserved POU-specific domain. PCR with primers PCR6-5 GAA-TTC-ATG-AAA-TCG-GAG-GCG-T (->) and PCR6-3 GTG-AGA-AGT-GAA-GAG-GAT-TGT-A (<-) yields a band of about 1.6 kb instead of 280 bp as in N2. Backcrossed more than 10 times; in addition, the left arm of LG I was recombined with lin-28 to remove the mutator locus.
TB1682 chEx1682. C. elegans chEx1682 [pLH070(qua-1(full-length)::GFP + rol-6(su1006)]. Rollers. Maintain under normal conditions; pick rollers. Reference: Hao et al. (2006) Dev Dyn 235:1469-81.
TB200 ceh-2(ch4) I. C. elegans Growth slightly retarded. Electropharyngeograms largely lack M3 spikes.
TB255 ceh-2(ch4) I; lin-15B&lin-15A(n765) X. C. elegans Growth slightly retarded. Electropharyngeograms largely lack M3 spikes. Temperature sensitive Muv.
TB526 ceh-14(ch1) X. C. elegans WT strain. ch1 deletes only intron sequences.
TB528 ceh-14(ch3) X. C. elegans PHA and PHB dye-filling defect. About 50% athermotactic. ch3 deletes exon 3, causes frameshift and premature stop. [NOTE: Miyazaki, et al. (2022) report that this strain carries the fln-2(ot611) mutation in the background.]

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
ch4 Allele deletion
ch1 Allele deletion
ch3 Allele deletion