Laboratory Information
| Name | ST View on WormBase |
|---|---|
| Allele designation | nc |
| Head | Shin Takagi |
| Institution | Nagoya University, Nagoya, Japan |
| Address | Group Development & Growth, Dept. Biology, Sch. Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya 464-8602 Japan |
| Gene classes | gcn plx ven |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| ST101 | ncIs1. | C. elegans | ncIs1 [eat-20::GFP + rol-6(su1006)]. Rollers. |
| ST13 | klf-3(nc13)/dpy-10(e128) unc-53(n569) II; him-8(e1489) IV. | C. elegans | Heterozygotes are WT and segregate WT, Dpy Uncs, and animals with muscle attachment defects and ventral cord displacement and detachment. Not well balanced. klf-3 was formerly known as mua-1. |
| ST14 | mua-5(nc14)/bli-6(sc16) egl-19(n582) unc-24(e138) IV. | C. elegans | Heterozygotes are WT and segregate WT, BliEglUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced. |
| ST15 | ncIs2 II; mua-5(nc15)/bli-6(sc16) egl-19(n582) unc-24(e138) IV. | C. elegans | ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, BliEglUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced. Neurons visualized with ncIs2. |
| ST16 | ncIs2 II; mua-6(nc16)/unc-3(e151) X. | C. elegans | ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, Uncs, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced. Neurons visualized with ncIs2. |
| ST17 | mua-5(nc17)/bli-6(sc16) egl-19(n582) unc-24(e138) IV. | C. elegans | Heterozygotes are WT and segregate WT, BliEglUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced. |
| ST18 | mua-5(nc18)/bli-6(sc16) egl-19(n582) unc-24(e138) IV. | C. elegans | Heterozygotes are WT and segregate WT, BliEglUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced. |
| ST2 | ncIs2 II. | C. elegans | ncIs2 [pH20::GFP + pBlueScript]. Expresses GFP in nearly all neurons. No morphological or behavioral phenotypes. |
| ST20 | ncIs2 II; mup-4(nc20)/sma-3(e491) unc-32(e189) III. | C. elegans | ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, SmaUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2. |
| ST21 | ncIs2 II; mup-4(nc21)/dpy-17(e164) unc-32(e189) III. | C. elegans | ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2. |
| ST22 | ncIs2 II; mup-4(nc22)/dpy-17(e164) unc-32(e189) III. | C. elegans | ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development or as embryos. Not well balanced. Neurons visualized with NcIs2. |
| ST2351 | ncEx2351. | C. elegans | ncEx2351 [unc-47p::Arch::eGFP + (pCFJ90) myo-2p::mCherry]. Pick GFP+ animals to maintain. Reference: Okazaki A, et al. PLoS One. 2012;7(5):e35370. |
| ST2365 | ncEx2365. | C. elegans | ncEx2365 [del-1p::Arch::eGFP + (pCFJ90) myo-2p::mCherry]. Pick GFP+ animals to maintain. Reference: Okazaki A, et al. PLoS One. 2012;7(5):e35370. |
| ST2371 | ncEx2371. | C. elegans | ncEx2371 [acr-5p::Arch::eGFP + (pCFJ90) myo-2p::mCherry]. Pick GFP+ animals to maintain. Reference: Okazaki A, et al. PLoS One. 2012;7(5):e35370. |
| ST25 | ven-1(nc25) V. | C. elegans | Ventral cord displacement and detachment. Muscle attachment defects. Some larval lethality. |
| ST26 | ven-1(nc26) V. | C. elegans | Ventral cord displacement and detachment. Muscle attachment defects. Some larval lethality. |
| ST28 | ven-1(nc28) V. | C. elegans | Ventral cord displacement and detachment. Muscle attachment defects. Some larval lethality. |
| ST29 | ven-2(nc29)/mnC1 [dpy-10(e128) unc-52(e444)] II; ncIs3 III. | C. elegans | ncIs3 [pH20::GFP + pBlueScript]. Expresses GFP in nearly all neurons. Heterozygotes are WT and segregate WT, DpyUncs, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. |
| ST30 | spon-1(nc30) ncIs2/dpy-10(e128) unc-53(n569) II. | C. elegans | ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUnc, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced. Neurons visualized with ncIs2. |
| ST3003 | ncEx3003. | C. elegans | ncEx3003 [hsp16-2p::Arch::eGFP + rol-6(su1006)]. Pick GFP+ animals to maintain. Reference: Okazaki A, et al. PLoS One. 2012;7(5):e35370. |
| ST3026 | ncEx3026. | C. elegans | ncEx3026 [aex-3p::Arch::eGFP + rol-6(su1006)]. Pick GFP+ animals to maintain. Reference: Okazaki A, et al. PLoS One. 2012;7(5):e35370. |
| ST3031 | ncEx3031. | C. elegans | ncEx3031 [myo-3p::Arch::eGFP + rol-6(su1006)]. Pick GFP+ animals to maintain. Reference: Okazaki A, et al. PLoS One. 2012;7(5):e35370. |
| ST3034 | ncEx3034. | C. elegans | ncEx3034 [F25B3.3p::Arch::eGFP + rol-6(su1006)]. Pick GFP+ animals to maintain. Reference: Okazaki A, et al. PLoS One. 2012;7(5):e35370. |
| ST3090 | ncEx3090. | C. elegans | ncEx3090 [tph-1p::Arch::eGFP + (pCFJ90) myo-2p::mCherry]. Pick GFP+mCherry+ animals to maintain. Reference: Okazaki A, et al. PLoS One. 2012;7(5):e35370. |
| ST35 | ncIs2 II; mup-4(nc35)/dpy-17(e164) unc-32(e189) III. | C. elegans | ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, DpyUncs, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest as embyros or in larval development. Not well balanced. Neurons visualized with ncIs2. |
| ST36 | plx-1(nc36) IV. | C. elegans | Various epidermal defects. In male tails, ray 1 is dislocated anteriorly. |
| ST53 | ncIs3 III; him-5(e1490) V. | C. elegans | ncIs3 [pH20::GFP + pBlueScript]. Expresses GFP in nearly all neurons. No morphological or behavioral phenotypes. |
| ST54 | plx-1(nc37) IV; him-5(e1490) V. | C. elegans | Various epidermal defects. In male tails, ray 1 is dislocated anteriorly. |
| ST6 | eat-20(nc4) | C. elegans | Starved appearance: shorter body length, pale intestine, reduced pharyngeal pumping, smaller brood size and extended egg-laing period. |
| ST60 | gcn-1(nc40) III. | C. elegans | Reduced brood size. Reduced phosphorylation level of eIF2alpa. Isolated as a suppressor of the ray1 phenotype of plx-1. |
| ST65 | ncIs13. | C. elegans | ncIs13 [ajm-1::GFP]. |
| ST66 | ncIs17. | C. elegans | ncIs17 contains [hsp-16.2::eGFP + pBluscript]. Superficially wild-type. |
| ST9005 | ncEx9005. | C. elegans | ncEx9005 [lin-32p::FLAG::let-363 + lin-32p::Myc::daf-15 + lin-32p::HA::rict-1 + hsp16-2p::plx-1 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. KpnI sites were added by PCR to let-363, daf-15 and rict-1 cDNAs and inserted into pPD49.26 containing lin-32p followed by the FLAG-, Myc- and HA-coding sequences to generate lin-32p::FLAG::let-363, lin-32p::Myc::daf-15 and lin-32p::HA::rict-1, respectively. Reference: Nukazuka A, et al. Nat Commun. 2011 Sep 27;2:484. |
| ST9007 | ncEx9007. | C. elegans | ncEx9007 [unc-54p::FLAG::let-363 + unc-54p::Myc::daf-15 + unc-54p::HA::rict-1 + hsp16-2p::plx-1 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. KpnI sites were added by PCR to let-363, daf-15 and rict-1 cDNAs and inserted into pPD49.26 containing inserted into pPD30.38 containing unc-54p followed by the FLAG-, Myc- and HA-coding sequences to generate unc-54p::FLAG::let-363, unc-54p::Myc::daf-15 and unc-54p::HA::rict-1, respectively. Reference: Nukazuka A, et al. Nat Commun. 2011 Sep 27;2:484. |
| ST9013 | ncEx9013. | C. elegans | ncEx9013 [lin-32p::FLAG::h4EBP1 + rol-6(su1006)]. Rollers. Pick Rollers to maintain. KpnI sites were added by PCR to h4EBP1 cDNA and inserted into pPD49.26 containing lin-32p followed by the FLAG-coding sequence to generate in-32p::FLAG::h4EBP1. Reference: Nukazuka A, et al. Nat Commun. 2011 Sep 27;2:484. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| nc37 | Allele | deletion | |
| nc13 | Allele | ||
| nc14 | Allele | ||
| nc15 | Allele | ||
| nc16 | Allele | substitution | nonsense |
| nc17 | Allele | ||
| nc18 | Allele | ||
| nc20 | Allele | ||
| nc21 | Allele | ||
| nc22 | Allele | ||
| nc25 | Allele | ||
| nc26 | Allele | ||
| nc28 | Allele | ||
| nc29 | Allele | ||
| nc30 | Allele | substitution | slient |
| nc35 | Allele | ||
| nc36 | Allele | deletion | |
| nc4 | Allele | deletion | |
| nc40 | Allele | deletion |