Laboratory Information

NameSM View on WormBase
Allele designationpx
HeadSusan E Mango
InstitutionUniversity of Basel
Address Mango Lab
Biozentrum Room 205
Cambridge 405602138
Switzerland
Website https://www.biozentrum.unibas.ch/research/researchgroups/group/unit/mango/
Gene classes htz  tlf  arle 

Strains contributed by this laboratory

Strain Genotype Species Description
SM1017 tlf-1(ok389)/unc-55(e402) blmp-1(s71) I. C. elegans Heterozygotes are wild type and segregate wild type, Dpy Uncs, and arrested early larva (L1s). Pick WT to maintain.
SM1052 zen-4(px47) dpy-20(e1282)/bli-6(sc16) unc-24(e138) C. elegans Heterozygotes are WT and segregate WT, Bli Uncs, and dead embryos and L1s (with Pun phenotype).
SM1366 mxl-2(tm1516) III. C. elegans No observable phenotype.
SM1480 mxl-2(tm1516) pha-1(e2123) III; him-8(e1489) IV. C. elegans Worms are viable at 15C and dead at 24C.
SM1508 mxl-2(tm1516) III; bar-1(ga80) X. C. elegans Most defects are similar to bar-1(ga80) single mutant animals [bar-1(ga80) hermaphrodites are usually Egl and often have a protruding vulva (Pvl), although approx. 40% of animals appear WT on plates. Also slightly Unc. In bar-1(ga80) hermaphrodites any of the six vulval precursor cells (P3.p - P8.p) can sometimes fuse with hyp7 without dividing, and P5.p - P7.p can adopt the tertiary cell fate instead of the primary or secondary fates. In addition, the neuroblast QL and its progeny migrate towards the anterior instead of the posterior, and the cell P12 usually adopts the fate of P11. bar-1(ga80) do mate, but poorly. bar-1 encodes a beta-catenin molecule and the ga80 mutation is predicted to cause an early truncation of the protein.] Increased severity of ray 1 displacement.
SM1584 mxl-2(tm1516) III; plx-1(nc37) IV; him-5(e1490) V; bar-1(ga80) X. C. elegans Hermaphrodites are sluggish and exhibit protruding vulva, ruptured vulva, or internally hatched progeny. Males move slower than WT and have disorganized tail rays.
SM1585 plx-1(nc37) IV; him-5(e1490) V; bar-1(ga80) X. C. elegans Hermaphrodites are sluggish and exhibit protruding vulva, ruptured vulva, or internally hatched progeny. Males move slower than WT and have disorganized tail rays.
SM1586 mxl-2(tm1516) III; plx-1(nc37) IV; him-5(e1490) V. C. elegans Hermaphrodites seem fine. Males have a high penetrance of anterior displacement of ray 1.
SM190 smg-1(cc546) I; pha-4(zu225) V. C. elegans Dies at 15-20C with mostly dead embyros and a few dead larvae. Grows best at 24C. Survives at 25C, but worms look sick (often small and clear) and have very reduced brood sizes.
SM481 pxIs10. C. elegans pxIs10 [pha-4::GFP::CAAX + rol-6(su1006)]. Roller line that has GFP localized to the plasma membrane of the pharynx, gut and rectal cells in embryos and the somatic gonad during L2-L3 larval stage and beyond. Reference: Portereiko MF & Mango SE. Dev Biol. 2001 May 15;233(2):482-94.
SM942 tbp-1(ok185)/qC1 [dpy-19(e1259) glp-1(q339)] III. C. elegans Heterozygotes are WT and segregate WT, Dpy(ts) Steriles and dead embryos/larvae.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
px47 Allele substitution