Laboratory Information
| Name | SL View on WormBase |
|---|---|
| Allele designation | eb |
| Head | L'Hernault, Steve |
| Institution | Emory University, Atlanta, GA |
| Address | Emory University RRC2062 1510 Clifton Rd NE Atlanta 30322 United States |
| Website | http://www.biology.emory.edu/research/LHernault/index.html |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| BA671 | spe-9(hc88) I. | C. elegans | Self-sterile at 25C. Maintain at 15C. |
| BA708 | spe-9(hc52) I. | C. elegans | Maintain at 15C. |
| BA714 | sDf5/spe-4(hc78) I. | C. elegans | Heterozygotes are WT and segregate WT heterozygotes, Sterile spe-4 homozygotes, and dead eggs (sDf5 homozygotes). |
| BA717 | spe-11(hc90) I; sDp2 (I;f). | C. elegans | Animals carrying the duplication WT. Animals which have lost the Dup are sterile. Maintain by picking fertile animals and checking for segregation of fertile and sterile progeny. |
| BA759 | hcDf1 IV; eEx25. | C. elegans | eEx25 [C13G4(cosmid) + XDM23(phage)]. Animals with eEx25 are WT. Pick wild-type to maintain. Animals which have lost eEx25 are Twitchers and Sterile (occasionally produce young). This cosmid lacks the 3' end of the unc-22 gene while XDM23 lacks the 5' end of unc-22, but following injection an extrachromosomal array was formed that included at least one functional unc-22 gene. |
| BA763 | spe-5(hc93) I; sDp2 (I;f). | C. elegans | Animals with the Duplications are WT. Animals which have lost the Duplication are Sterile. |
| BA783 | spe-12(hc76) I. | C. elegans | Maintain by mating. Hermaphrodites produce nonfunctional sperm and are fertilization defective. Self-fertility is <1% at 16C or 25C. Males are fertile. |
| BA785 | spe-8(hc40) I. | C. elegans | Hermaphrodites produce nonfunctional, nonmotile sperm with uniformly aberrant pseudopods. Male sperm normal. Self-fertility is <1% at 16C or 25C. Maintain by mating. |
| BA794 | spe-13(hc137) I. | C. elegans | Temperature sensitive. Maintain at 15C. |
| BA811 | sDf5/spe-4(hc78) unc-15(e73) I. | C. elegans | Heterozygotes are WT and segregate WT heterozygotes, Sterile Unc spe-4 unc-15 homozygotes, and dead eggs (sDf5 homozygotes). Pick wild-type to maintain. |
| SL1138 | spe-42(tn1231) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). | C. elegans | Heterozygotes are Unc and express myo-2::GFP in the pharynx. spe-42(tn1231) homozygotes are non-Unc and non-GFP. spe-42(tn1231) produces <1 self progeny at 16C and no self progeny at 25C. The Spe defect can be rescued by WT sperm. Homozygous nT1[unc-?(n754) let-? qIs50] are embryonic lethal. |
| SL3 | spe-15(hc75) I. | C. elegans | Largely sterile, even at permissive temperature (15C). |
| SL305 | spe-26(eb8) IV/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygotes are Unc and segregate Unc and WT which are Spe (they lay only oocytes on the plate). Maintain by picking Unc. |
| SL536 | dxDf2/spe-9(eb19) unc-101(m1) I. | C. elegans | Heterozygotes are Unc and segregate Uncs, Sterile Uncs and dead eggs. Strain is sick and grows slowly. dxDf2 fails to complement unc-54, so it could delete the entire right arm of LG I. |
| SL740 | dpy-2(e8) unc-4(e120)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT with major GFP signal in pharynx. Segregates Dpy GFP+ mIn1 homozygotes and GFP- DpyUncs. |
| SL753 | spe-39(tx12) V/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygote are fertile and Unc. spe-39(tx12) homozygotes are self-sterile due to defects in spermatogenesis. |
| SL754 | spe-39(eb9) V/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygote are fertile and Unc. spe-39(eb9) homozygotes are self-sterile due to defects in spermatogenesis. |
| SL940 | wee-1.3(q89eb94) unc-4(e120)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT with major GFP signal in pharynx. Segregates Dpy GFP+ mIn1 homozygotes and GFP- Uncs which are viable but lay oocytes (lay viable embryos if mated to WT males). Strain has a Him phenotype. 3.4% males. Mutant males have abnormal sperm. Class 1 suppressor. |
| SL958 | unc-42(e270) spe-42(eb5) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). | C. elegans | Heterozygotes are Unc and express myo-2::GFP in the pharynx. unc-42(e270) spe-42(eb5) homozygotes are Unc and non-GFP. Whle unc-42(e270) spe-42(eb5) self progeny have not been quantified, spe-42(eb5) alone produces 9 self progeny at 16C and <1 self progeny at 25C. The Spe defect can be rescued by WT sperm. Homozygous nT1[unc-?(n754) let-? qIs50] are embryonic lethal. |
| SL978 | wee-1.3(q89eb60) unc-4(e120)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT with major GFP signal in pharynx. Segregates Dpy GFP+ mIn1 homozygotes and GFP- Uncs which are viable but lay inviable embryos (lay viable embyros if mated to WT males). Strain has a Him phenotype. 0.4% males. Mutant males are fertile. Class 2 suppressor. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| eb52 | Allele | substitution | |
| eb44 | Allele | deletion | |
| eb8 | Allele | substitution | nonsense |
| eb19 | Allele | substitution | nonsense |
| eb9 | Allele | substitution | nonsense |
| eb94 | Allele | substitution | |
| eb5 | Allele | substitution | nonsense |
| eb60 | Allele | substitution | nonsense |