Laboratory Information

NameSL View on WormBase
Allele designationeb
HeadL'Hernault, Steve
InstitutionEmory University, Atlanta, GA
Address Emory University
RRC2062
1510 Clifton Rd NE
Atlanta 30322
United States
Website http://www.biology.emory.edu/research/LHernault/index.html
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
BA671 spe-9(hc88) I. C. elegans Self-sterile at 25C. Maintain at 15C.
BA708 spe-9(hc52) I. C. elegans Maintain at 15C.
BA714 sDf5/spe-4(hc78) I. C. elegans Heterozygotes are WT and segregate WT heterozygotes, Sterile spe-4 homozygotes, and dead eggs (sDf5 homozygotes).
BA717 spe-11(hc90) I; sDp2 (I;f). C. elegans Animals carrying the duplication WT. Animals which have lost the Dup are sterile. Maintain by picking fertile animals and checking for segregation of fertile and sterile progeny.
BA759 hcDf1 IV; eEx25. C. elegans eEx25 [C13G4(cosmid) + XDM23(phage)]. Animals with eEx25 are WT. Pick wild-type to maintain. Animals which have lost eEx25 are Twitchers and Sterile (occasionally produce young). This cosmid lacks the 3' end of the unc-22 gene while XDM23 lacks the 5' end of unc-22, but following injection an extrachromosomal array was formed that included at least one functional unc-22 gene.
BA763 spe-5(hc93) I; sDp2 (I;f). C. elegans Animals with the Duplications are WT. Animals which have lost the Duplication are Sterile.
BA783 spe-12(hc76) I. C. elegans Hermaphrodites produce nonfunctional sperm and are fertilization defective. Self-fertility is <1% at 16C or 25C. Males are fertile.
BA785 spe-8(hc40) I. C. elegans Hermaphrodites produce nonfunctional, nonmotile sperm with uniformly aberrant pseudopods. Male sperm normal. Self-fertility is <1% at 16C or 25C. Maintain by mating.
BA794 spe-13(hc137) I. C. elegans Temperature sensitive. Maintain at 15C.
BA811 sDf5/spe-4(hc78) unc-15(e73) I. C. elegans Heterozygotes are WT and segregate WT heterozygotes, Sterile Unc spe-4 unc-15 homozygotes, and dead eggs (sDf5 homozygotes). Pick wild-type to maintain.
SL1138 spe-42(tn1231) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). C. elegans Heterozygotes are Unc and express myo-2::GFP in the pharynx. spe-42(tn1231) homozygotes are non-Unc and non-GFP. spe-42(tn1231) produces <1 self progeny at 16C and no self progeny at 25C. The Spe defect can be rescued by WT sperm. Homozygous nT1[unc-?(n754) let-? qIs50] are embryonic lethal.
SL3 spe-15(hc75) I. C. elegans Largely sterile, even at permissive temperature (15C).
SL305 spe-26(eb8) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Unc and WT which are Spe (they lay only oocytes on the plate). Maintain by picking Unc.
SL536 dxDf2/spe-9(eb19) unc-101(m1) I. C. elegans Heterozygotes are Unc and segregate Uncs, Sterile Uncs and dead eggs. Strain is sick and grows slowly. dxDf2 fails to complement unc-54, so it could delete the entire right arm of LG I.
SL645 wee-1.3(q89) unc-4(e120)/dpy-2(e8) unc-4(e120) II. C. elegans Dominant Spe.
SL740 dpy-2(e8) unc-4(e120)/mIn1 [dpy-10(e128) mIs14] II. C. elegans Heterozygotes are WT with major GFP signal in pharynx. Segregates Dpy GFP+ mIn1 homozygotes and GFP- DpyUncs.
SL753 spe-39(tx12) V/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygote are fertile and Unc. spe-39(tx12) homozygotes are self-sterile due to defects in spermatogenesis.
SL754 spe-39(eb9) V/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygote are fertile and Unc. spe-39(eb9) homozygotes are self-sterile due to defects in spermatogenesis.
SL940 wee-1.3(q89eb94) unc-4(e120)/mIn1 [dpy-10(e128) mIs14] II. C. elegans Heterozygotes are WT with major GFP signal in pharynx. Segregates Dpy GFP+ mIn1 homozygotes and GFP- Uncs which are viable but lay oocytes (lay viable embryos if mated to WT males). Strain has a Him phenotype. 3.4% males. Mutant males have abnormal sperm. Class 1 suppressor.
SL958 unc-42(e270) spe-42(eb5) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). C. elegans Heterozygotes are Unc and express myo-2::GFP in the pharynx. unc-42(e270) spe-42(eb5) homozygotes are Unc and non-GFP. Whle unc-42(e270) spe-42(eb5) self progeny have not been quantified, spe-42(eb5) alone produces 9 self progeny at 16C and <1 self progeny at 25C. The Spe defect can be rescued by WT sperm. Homozygous nT1[unc-?(n754) let-? qIs50] are embryonic lethal.
SL978 wee-1.3(q89eb60) unc-4(e120)/mIn1 [dpy-10(e128) mIs14] II. C. elegans Heterozygotes are WT with major GFP signal in pharynx. Segregates Dpy GFP+ mIn1 homozygotes and GFP- Uncs which are viable but lay inviable embryos (lay viable embyros if mated to WT males). Strain has a Him phenotype. 0.4% males. Mutant males are fertile. Class 2 suppressor.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
eb52 Allele substitution
eb44 Allele deletion
eb8 Allele substitution nonsense
eb19 Allele substitution nonsense
eb9 Allele substitution nonsense
eb94 Allele substitution
eb5 Allele substitution nonsense
eb60 Allele substitution nonsense