Laboratory Information
| Name | SA View on WormBase |
|---|---|
| Allele designation | tj |
| Head | Asako Sugimoto |
| Institution | Tohoku University, Sendai, Japan |
| Address | Laboratory of Developmental Dynamics, Tohoku University 2-2-1 Katahira Aoba-ku Sendai 980-8577 Japan |
| Website | http://www.lifesci.tohoku.ac.jp/sugimoto_lab/english/index.html |
| Gene classes | cdl daz ebp eya fzr gmn pph ctr leo pafo rtfo |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| JR36 | cdl-1(e2510)/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | Heterozygotes are WT and segregate WT, dead eggs (homozygous cdl-1(e2510), and Dpy Unc. e2510 carries a missense mutation at the 249th amino acid from Pro to Ser. |
| SA104 | eya-1(tm759)/hIn1 [unc-54(h1040)] I. | C. elegans | Heterozygotes are WT. hIn1 homozygotes are Unc. tm759 homozygous arrest as early larva with incomplete penetrance (60% at 25C, 50% at 20C, 30% at 18C, and 50% at 15C). Viable tm759 homozygotes show various postembryonic phenotypes (Unc, Dpy, Egl, Mig, Muv). |
| SA115 | unc-119(ed3) III; tjIs1. | C. elegans | tjIs1[pie-1::GFP::rho-1 + unc-119(+)]. GFP-RHO-1 is found at the basement membrane location in germline and early embryos and at the anterior cortical region in one-cell embyros. Small brood size. pID3.01-rho-1. Integration site unknown. |
| SA125 | unc-119(ed3) III; tjIs4. | C. elegans | tjIs4 [pie-1::GFP::ect-2 + unc-119(+)]. GFP-ECT-2 is found at the anterior cortex in early embyros. pID3.01-ect-2. Integration site unknown. Maintain at 25C to avoid transgene silencing. |
| SA127 | unc-119(ed3) III; tjIs5. | C. elegans | tjIs5 [pie-1p::GFP::plk-1 + unc-119(+)]. GFP::PLK-1 is found at the mitotic centrosomes, kinetochores, and midbody in early embyros. pID3.01-plk-1. Integration site unknown. Maintain at 25C to avoid transgene silencing. |
| SA131 | unc-119(ed3) III; tjIs6. | C. elegans | tjIs6 [pie-1p::GFP::cdc-42 + unc-119(+)]. GFP::CDC-42 localizes to basement membranes in both embryos and germline, and to the anterior cortex of one-cell embryos. pID3.01-cdc-42. Integration site unknown. |
| SA141 | unc-119(ed3) III; tjIs8. | C. elegans | tjIs8 [pie-1p::GFP::ebp-1 + unc-119(+)]. GFP::ebp-1 is found at the tips of growing microtubules in early embryos. pID3.01-ebp-1. Integration site unknown. |
| SA146 | eya-1(ok654) I. | C. elegans | ok654 homozygous arrest as early larva with incomplete penetrance (80% at 25C, 30% at 20C, 40% at 18C, and 50% at 15C). Viable ok654 homozygotes show various postembryonic phenotypes (Unc, Dpy, Egl, Mig, Muv). |
| SA25 | daz-1(tj3)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT (GFP+) and segregate WT (GFP+), Dpys (GFP+) and Steriles. |
| SA250 | tjIs54; tjIs57. | C. elegans | tjIs54 [pie-1p::GFP::tbb-2 + pie-1p::2xmCherry::tbg-1 + unc-119(+)]. tjIs57 [pie-1p::mCherry::his-48 + unc-119(+)]. Maintain at 25C to avoid transgene silencing. Reference: Toya M, et al. Methods Cell Biol. 2010;97:359-72. |
| SA4 | cdl-1(w37)/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | Heterozygotes are WT and segregate WT, dead eggs (homozygous cdl-1(w37)), and DpyUnc. w37 carries a 4.7kb deletion that removed the entire cdl-1 ORF and part of the neighboring ORF (T19E10.1), with a small insetion of about 60 bp. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| tj3 | Allele | ||
| tj11 | Allele | deletion | |
| tj12 | Allele | deletion |