Caenorhabditis Genetics Center (CGC)

Laboratory Information

NameRT View on WormBase
Allele designationpw
HeadBarth D Grant
InstitutionRutgers University, Piscataway, NJ
Address Rutgers University
Nelson Labs A231
604 Allison Rd
Piscataway 08502
United States
Website http://lifesci.rutgers.edu/grantlab/
Gene classes amph  chc  clic  eea  ehbp  rabn  rabx  sdpn  snx  denn  tgn 

Strains contributed by this laboratory

Strain Genotype Species Description
DH1033 sqt-1(sc103) II; bIs1 X. C. elegans bIs1[vit-2::GFP + rol-6(su1006)].
DH1201 rme-1(b1045) V. C. elegans Viable. Progressive vacuolation of intestine beginning in L4. Endocytosis defects in oocytes and coelomocytes.
DH1206 rme-8(b1023) I. C. elegans Temperature sensitive lethal. Maintain at 15C. Endocytosis defects in oocytes (non-ts) and coelomocytes (ts).
DH1230 chc-1(b1025) III. C. elegans Temperature sensitive lethal. Endocytosis defective. Maintain at 15C. AKA: rme-3.
DH1336 bIs34. C. elegans bIs34 [rme-8::GFP + rol-6(su1006)].
DH1370 rme-6(b1014) X. C. elegans Accumulation of yolk GFP in pseudocoelom suggesting decrease or absence of coelomocyte mediated endocytosis. Abundant pseudocoelomic yolk.
RT1043 unc-119(ed3) III; pwIs403. C. elegans pwIs403 [pie-1p::mCherry::rab-5 + unc-119(+)]. mCherry::RAB-5 provides a marker of early endosomes in the germline and in early embryos. Superficially wild-type. For best expression maintain propagate at 25 degrees.
RT122 unc-119(ed3) III; pwIs20. C. elegans pwIs20 contains [pie-1p::GFP::rab-5 + unc-119(+)]. Superficially wild-type. Maintain at 20 degrees.
RT130 pwIs23. C. elegans pwIs23 [vit-2::GFP].
RT1315 unc-119(ed3) III; pwIs503. C. elegans pwIs503 [vha-6p::mans::GFP + Cbr-unc-119(+)]. This strain expresses an intestine-specific GFP marker for the Golgi apparatus (a fragment of C. elegans alpha-mannosidase II (F58H1.1, first 82 aa including signal sequence/TM-anchor domain as in Rolls et al., 2002). This fusion protein is expressed under the control of the vha-6 promoter.
RT17 rab-10(dx2) I. C. elegans Temperature sensitive. Maintain at 15C.
RT2 rab-10(q373) I. C. elegans Large intestinal vacuoles.
RT206 rab-35(b1013) III. C. elegans Yolk endocytosis defective.
RT258 unc-119(ed3) III; pwIs50. C. elegans pwIs50 [lmp-1::GFP + Cbr-unc-119(+)].
RT311 unc-119(ed3) III; pwIs69. C. elegans pwIs69 [vha-6p::GFP::rab-11 + unc-119(+)]. Likely integrated into LG X (based on user observations).
RT327 unc-119(ed3) III; pwIs72. C. elegans pwIs72 [vha-6p::GFP::rab-5 + Cbr-unc-119(+)]. Likely integrated in LG II.
RT3351 pitr-1(pw8[pitr-1::gfp]) IV. C. elegans GFP tag was inserted into the endogenous pitr-1 locus using CRISPR/Cas9 engineering. Reference: Balklava Z, et al. Genetics. 2016 Sep; 204(1): 153–162. PMID: 27449055.
RT3574 lin-15B&lin-15A(n765) X; ihIs35. C. elegans ihIs35 [yap-1::GFP::unc-54 3'UTR + lin-15(+)]. Translational yap-1::GFP fusion containing 5 kb of upstream regulatory sequence and 2.5kb coding genomic sequence; inserted into pPD95.75. Integration of yap-1::GFP extrachromosomal array was performed by the UV irradiation method. Reference: Iwasa H, et al. Exp Cell Res. 2013 Apr 15;319(7):931-45.
RT362 rme-4(b1001) X; pwIs23. C. elegans pwIs32 [vit-2::GFP]. Yolk endocytosis defective.
RT368 unc-119(ed3) III; pwIs98. C. elegans pwIs98 [YP170::tdimer2 + unc-119(+)]. Reference: Sato M et al. (2008) EMBO J. 27(8):1183-96.
RT408 unc-119(ed3) III; pwIs116. C. elegans pwIs116 [rme-2p::rme-2::GFP::rme-2 3'UTR + unc-119(+)]. Superficially wild-type. Maintain at 20-25C to reduce silencing of the array.
RT476 unc-119(ed3) III; pwIs170. C. elegans pwIs170 [vha6p::GFP::rab-7 + Cbr-unc-119(+)].
RT525 unc-119(ed3) III; pwIs206. C. elegans pwIs206 [vha6p::GFP::rab-10 + Cbr-unc-119(+)].
RT688 unc-119(ed3) III; pwIs28. C. elegans pwIs28 [pie-1p::cav-1::GFP(7) + unc-119(+)].
RT99 rme-4(b1001); bIs1. C. elegans bIs1 [vit-2::GFP + rol-6(su1006)]. Rollers. Yolk endocytosis defective. Reference: Sato M, et al. EMBO J. 2008 Apr 23;27(8):1183-96.
This laboratory hasn't submitted any alleles to the CGC.