Laboratory Information

NameRT View on WormBase
Allele designationpw
HeadBarth D Grant
InstitutionRutgers University, Piscataway, NJ
Address Rutgers University
Nelson Labs A231
604 Allison Rd
Piscataway 08502
United States
Gene classes amph  chc  clic  eea  ehbp  rabn  rabx  sdpn  snx  denn  tgn 

Strains contributed by this laboratory

Strain Genotype Species Description
DH1033 sqt-1(sc103) II; bIs1 X. C. elegans bIs1[vit-2::GFP + rol-6(su1006)].
DH1201 rme-1(b1045) V. C. elegans Viable. Progressive vacuolation of intestine beginning in L4. Endocytosis defects in oocytes and coelomocytes.
DH1206 rme-8(b1023) I. C. elegans Temperature sensitive lethal. Maintain at 15C. Endocytosis defects in oocytes (non-ts) and coelomocytes (ts).
DH1230 chc-1(b1025) III. C. elegans Temperature sensitive lethal. Endocytosis defective. Maintain at 15C. AKA: rme-3.
DH1336 bIs34. C. elegans bIs34 [rme-8::GFP + rol-6(su1006)].
DH1370 rme-6(b1014) X. C. elegans Accumulation of yolk GFP in pseudocoelom suggesting decrease or absence of coelomocyte mediated endocytosis. Abundant pseudocoelomic yolk.
RT1043 unc-119(ed3) III; pwIs403. C. elegans pwIs403 [pie-1p::mCherry::rab-5 + unc-119(+)]. mCherry::RAB-5 provides a marker of early endosomes in the germline and in early embryos. Superficially wild-type. For best expression maintain propagate at 25 degrees.
RT122 unc-119(ed3) III; pwIs20. C. elegans pwIs20 contains [pie-1p::GFP::rab-5 + unc-119(+)]. Superficially wild-type. Maintain at 20 degrees.
RT130 pwIs23. C. elegans pwIs23 [vit-2::GFP].
RT1315 unc-119(ed3) III; pwIs503. C. elegans pwIs503 [vha-6p::mans::GFP + Cbr-unc-119(+)]. This strain expresses an intestine-specific GFP marker for the Golgi apparatus (a fragment of C. elegans alpha-mannosidase II (F58H1.1, first 82 aa including signal sequence/TM-anchor domain as in Rolls et al., 2002). This fusion protein is expressed under the control of the vha-6 promoter.
RT17 rab-10(dx2) I. C. elegans Temperature sensitive. Maintain at 15C.
RT2 rab-10(q373) I. C. elegans Large intestinal vacuoles.
RT206 rab-35(b1013) III. C. elegans Yolk endocytosis defective.
RT258 unc-119(ed3) III; pwIs50. C. elegans pwIs50 [lmp-1::GFP + Cbr-unc-119(+)].
RT311 unc-119(ed3) III; pwIs69. C. elegans pwIs69 [vha-6p::GFP::rab-11 + unc-119(+)]. Likely integrated into LG X (based on user observations).
RT327 unc-119(ed3) III; pwIs72. C. elegans pwIs72 [vha-6p::GFP::rab-5 + Cbr-unc-119(+)]. Likely integrated in LG II.
RT3351 pitr-1(pw8[pitr-1::gfp]) IV. C. elegans GFP tag was inserted into the endogenous pitr-1 locus using CRISPR/Cas9 engineering. Reference: Balklava Z, et al. Genetics. 2016 Sep; 204(1): 153–162. PMID: 27449055.
RT3574 lin-15B&lin-15A(n765) X; ihIs35. C. elegans ihIs35 [yap-1::GFP::unc-54 3'UTR + lin-15(+)]. Translational yap-1::GFP fusion containing 5 kb of upstream regulatory sequence and 2.5kb coding genomic sequence; inserted into pPD95.75. Integration of yap-1::GFP extrachromosomal array was performed by the UV irradiation method. Reference: Iwasa H, et al. Exp Cell Res. 2013 Apr 15;319(7):931-45.
RT362 rme-4(b1001) X; pwIs23. C. elegans pwIs32 [vit-2::GFP]. Yolk endocytosis defective.
RT368 unc-119(ed3) III; pwIs98. C. elegans pwIs98 [YP170::tdimer2 + unc-119(+)]. Reference: Sato M et al. (2008) EMBO J. 27(8):1183-96.
RT408 unc-119(ed3) III; pwIs116. C. elegans pwIs116 [rme-2p::rme-2::GFP::rme-2 3'UTR + unc-119(+)]. Superficially wild-type. Maintain at 20-25C to reduce silencing of the array.
RT476 unc-119(ed3) III; pwIs170. C. elegans pwIs170 [vha6p::GFP::rab-7 + Cbr-unc-119(+)].
RT525 unc-119(ed3) III; pwIs206. C. elegans pwIs206 [vha6p::GFP::rab-10 + Cbr-unc-119(+)].
RT688 unc-119(ed3) III; pwIs28. C. elegans pwIs28 [pie-1p::cav-1::GFP(7) + unc-119(+)].
RT99 rme-4(b1001); bIs1. C. elegans bIs1 [vit-2::GFP + rol-6(su1006)]. Rollers. Yolk endocytosis defective. Reference: Sato M, et al. EMBO J. 2008 Apr 23;27(8):1183-96.
This laboratory hasn't submitted any alleles to the CGC.