Laboratory Information
Name | RT View on WormBase |
---|---|
Allele designation | pw |
Head | Barth D Grant |
Institution | Rutgers University, Piscataway, NJ |
Address | Rutgers University Nelson Labs A231 604 Allison Rd Piscataway 08502 United States |
Website | http://lifesci.rutgers.edu/grantlab/ |
Gene classes | amph chc clic eea ehbp rabn rabx sdpn snx denn tgn |
Strains contributed by this laboratory
Strain | Genotype | Species | Description |
---|---|---|---|
DH1033 | sqt-1(sc103) II; bIs1 X. | C. elegans | bIs1[vit-2::GFP + rol-6(su1006)]. |
DH1201 | rme-1(b1045) V. | C. elegans | Viable. Progressive vacuolation of intestine beginning in L4. Endocytosis defects in oocytes and coelomocytes. |
DH1206 | rme-8(b1023) I. | C. elegans | Temperature sensitive lethal. Maintain at 15C. Endocytosis defects in oocytes (non-ts) and coelomocytes (ts). |
DH1230 | chc-1(b1025) III. | C. elegans | Temperature sensitive lethal. Endocytosis defective. Maintain at 15C. AKA: rme-3. |
DH1336 | bIs34. | C. elegans | bIs34 [rme-8::GFP + rol-6(su1006)]. |
DH1370 | rme-6(b1014) X. | C. elegans | Accumulation of yolk GFP in pseudocoelom suggesting decrease or absence of coelomocyte mediated endocytosis. Abundant pseudocoelomic yolk. |
RT1043 | unc-119(ed3) III; pwIs403. | C. elegans | pwIs403 [pie-1p::mCherry::rab-5 + unc-119(+)]. mCherry::RAB-5 provides a marker of early endosomes in the germline and in early embryos. Superficially wild-type. For best expression maintain propagate at 25 degrees. |
RT122 | unc-119(ed3) III; pwIs20. | C. elegans | pwIs20 contains [pie-1p::GFP::rab-5 + unc-119(+)]. Superficially wild-type. Maintain at 20 degrees. |
RT130 | pwIs23. | C. elegans | pwIs23 [vit-2::GFP]. |
RT1315 | unc-119(ed3) III; pwIs503. | C. elegans | pwIs503 [vha-6p::mans::GFP + Cbr-unc-119(+)]. This strain expresses an intestine-specific GFP marker for the Golgi apparatus (a fragment of C. elegans alpha-mannosidase II (F58H1.1, first 82 aa including signal sequence/TM-anchor domain as in Rolls et al., 2002). This fusion protein is expressed under the control of the vha-6 promoter. |
RT17 | rab-10(dx2) I. | C. elegans | Temperature sensitive. Maintain at 15C. |
RT2 | rab-10(q373) I. | C. elegans | Large intestinal vacuoles. |
RT206 | rab-35(b1013) III. | C. elegans | Yolk endocytosis defective. |
RT258 | unc-119(ed3) III; pwIs50. | C. elegans | pwIs50 [lmp-1::GFP + Cbr-unc-119(+)]. |
RT311 | unc-119(ed3) III; pwIs69. | C. elegans | pwIs69 [vha6p::GFP::rab-11 + unc-119(+)]. Likely integrated into LG X (based on user observations). |
RT327 | unc-119(ed3) III; pwIs72. | C. elegans | pwIs72 [vha-6p::GFP::rab-5 + Cbr-unc-119(+)]. Likely integrated in LG II. |
RT3574 | lin-15B&lin-15A(n765) X; ihIs35. | C. elegans | ihIs35 [yap-1::GFP::unc-54 3'UTR + lin-15(+)]. Translational yap-1::GFP fusion containing 5 kb of upstream regulatory sequence and 2.5kb coding genomic sequence; inserted into pPD95.75. Integration of yap-1::GFP extrachromosomal array was performed by the UV irradiation method. Reference: Iwasa H, et al. Exp Cell Res. 2013 Apr 15;319(7):931-45. |
RT362 | rme-4(b1001) X; pwIs23. | C. elegans | pwIs32 [vit-2::GFP]. Yolk endocytosis defective. |
RT368 | unc-119(ed3) III; pwIs98. | C. elegans | pwIs98 [YP170::tdimer2 + unc-119(+)]. Reference: Sato M et al. (2008) EMBO J. 27(8):1183-96. |
RT408 | unc-119(ed3) III; pwIs116. | C. elegans | pwIs116 [rme-2p::rme-2::GFP::rme-2 3'UTR + unc-119(+)]. Superficially wild-type. Maintain at 20-25C to reduce silencing of the array. |
RT476 | unc-119(ed3) III; pwIs170. | C. elegans | pwIs170 [vha6p::GFP::rab-7 + Cbr-unc-119(+)]. |
RT525 | unc-119(ed3) III; pwIs206. | C. elegans | pwIs206 [vha6p::GFP::rab-10 + Cbr-unc-119(+)]. |
RT688 | unc-119(ed3) III; pwIs28. | C. elegans | pwIs28 [pie-1p::cav-1::GFP(7) + unc-119(+)]. |
RT99 | rme-4(b1001); bIs1. | C. elegans | bIs1 [vit-2::GFP + rol-6(su1006)]. Rollers. Yolk endocytosis defective. Reference: Sato M, et al. EMBO J. 2008 Apr 23;27(8):1183-96. |
This laboratory hasn't submitted any alleles to the CGC.