Laboratory Information

NameRP View on WormBase
Allele designationtr
HeadPeter Roy
InstitutionUniversity of Toronto
Address University of Toronto
Room 1250
160 College Street
Toronto M5S 3E1
Canada
Website http://individual.utoronto.ca/proy/index.htm
Gene classes madd 

Strains contributed by this laboratory

Strain Genotype Species Description
PRJ112 mutEx70. C. elegans mutEx70 [pmk-1::GFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. [NOTE: This strain has been incorrectly referred to as RP112 muEx70.] Reference: Mertensk├Âtter A, et al. Cell Stress Chaperones. 2013 May;18(3):293-306.
RP1 trIs10. C. elegans trIs10 [myo-3p::MB::YFP + myo-2p::YFP + ceh-23::HcRed + unc-25::DsRed + unc-129nsp::CFP].
RP1416 madd-2(tr129) V. C. elegans Midline-oriented migration defects including muscle arm extension and HSN, AVM, and PVM axon guidance defects. Reference: Alexander M, et al. Dev Cell. 2010 Jun 15;18(6):961-72.
RP247 trIs30. C. elegans trIs30 [him-4p::MB::YFP + hmr-1b::DsRed2 + unc-129nsp::DsRed2].
RP3439 tris113. C elegans trIs113 [abu-14p::abu-14::GFP + rol-6(su1006)]. Rollers. Integrated line generated from an extrachromosomal array provided by David Raizen. References: George-Raizen JB, et al. Biol Open. 2014 Oct 31;3(11):1139-49. PMID: 25361578. Kamal M, et al. bioRxiv 2022.04.11.487937; doi: https://doi.org/10.1101/2022.04.11.487937.
RP3497 idpc-1(knu1089[Y47D3B.6::mGreenLantern]) III. C elegans mGreenLantern tag inserted at the C-terminus of the endogenous idpc-1/Y47D3B.6 locus. mGreenLantern expression in the pharyngeal cuticle. Reference: Kamal M, et al. "A Spatiotemporal Reconstruction of the C. elegans Pharyngeal Cuticle Reveals a Structure Rich in Phase-Separating Proteins." https://www.biorxiv.org/content/10.1101/2022.03.11.483951v1
RP3498 trEx1001. C elegans trEx1001 [idpb-3p::idpb-3::mNeonGreen + myo-2p::mCherry]. Pick animals with mCherry+ pharynx to maintain. Generated in N2 background. Reference: Kamal M, et al. bioRxiv 2022.03.11.483951; doi: https://doi.org/10.1101/2022.03.11.483951.
RP3510 trEx1010. C elegans trEx1010 [pgp-14p::sms-5B(genomic)::Flag::mCherry + pgp-14p::YFP::pgp-14]. Pick animals with mCherry+ pharynx to maintain. Fluorescence is more apparent in older animals (late stage larvae to young adults). Generated in N2 background. Reference: Kamal M, et al. bioRxiv 2022.03.11.483951; doi: https://doi.org/10.1101/2022.03.11.483951.
RP3513 trEx1006. C elegans trEx1006 [fipr-4p::fipr-4::mNeonGreen + myo-2p::mCherry]. Pick animals with mCherry+ pharynx to maintain. Generated in N2 background. Reference: Kamal M, et al. bioRxiv 2022.03.11.483951; doi: https://doi.org/10.1101/2022.03.11.483951.
RP3515 trEx1008. C elegans trEx1008 [idpa-3p::idpa-3::mNeonGreen + myo-2p::mCherry]. Pick animals with mCherry+ pharynx to maintain. Generated in N2 background. Reference: Kamal M, et al. bioRxiv 2022.03.11.483951; doi: https://doi.org/10.1101/2022.03.11.483951.
RP582 egl-19(tr69) IV. C. elegans Nemadipine-A resistant.
RP583 egl-19(tr70) IV. C. elegans Mildly myotonic. Nemadipine-A resistant.
RP584 egl-19(tr71) IV. C. elegans Nemadipine-A resistant.
RP585 egl-19(tr72) IV. C. elegans Myotonic. Nemadipine-A resistant.
RP586 egl-19(tr73) IV. C. elegans Mildly myotonic. Nemadipine-A resistant.
RP828 madd-2(tr103) V. C. elegans Midline-oriented migration defects including muscle arm extension and HSN, AVM, and PVM axon guidance defects. Reference: Alexander M, et al. Dev Cell. 2010 Jun 15;18(6):961-72.
SD1075 gaIs146. C. elegans gaIs146 [(pPRSK9) myo-3p::FLAG::pab-1 + sur-5::GFP]. Slightly slower growth than WT.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
tr129 Allele substitution nonsense
tr69 Allele substitution
tr70 Allele substitution
tr71 Allele substitution
tr72 Allele substitution
tr73 Allele substitution
tr103 Allele substitution nonsense