Laboratory Information
| Name | RP View on WormBase |
|---|---|
| Allele designation | tr |
| Head | Peter Roy |
| Institution | University of Toronto |
| Address | University of Toronto Room 1250 160 College Street Toronto M5S 3E1 Canada |
| Website | http://individual.utoronto.ca/proy/index.htm |
| Gene classes | madd |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| PRJ112 | mutEx70. | C. elegans | mutEx70 [pmk-1::GFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. [NOTE: This strain has been incorrectly referred to as RP112 muEx70.] Reference: Mertenskötter A, et al. Cell Stress Chaperones. 2013 May;18(3):293-306. |
| RP1 | trIs10. | C. elegans | trIs10 [myo-3p::MB::YFP + myo-2p::YFP + ceh-23::HcRed + unc-25::DsRed + unc-129nsp::CFP]. |
| RP1416 | madd-2(tr129) V. | C. elegans | Midline-oriented migration defects including muscle arm extension and HSN, AVM, and PVM axon guidance defects. Reference: Alexander M, et al. Dev Cell. 2010 Jun 15;18(6):961-72. |
| RP247 | trIs30. | C. elegans | trIs30 [him-4p::MB::YFP + hmr-1b::DsRed2 + unc-129nsp::DsRed2]. |
| RP3439 | tris113. | C elegans | trIs113 [abu-14p::abu-14::GFP + rol-6(su1006)]. Rollers. Integrated line generated from an extrachromosomal array provided by David Raizen. References: George-Raizen JB, et al. Biol Open. 2014 Oct 31;3(11):1139-49. PMID: 25361578. Kamal M, et al. bioRxiv 2022.04.11.487937; doi: https://doi.org/10.1101/2022.04.11.487937. |
| RP3497 | idpc-1(knu1089[Y47D3B.6::mGreenLantern]) III. | C elegans | mGreenLantern tag inserted at the C-terminus of the endogenous idpc-1/Y47D3B.6 locus. mGreenLantern expression in the pharyngeal cuticle. Reference: Kamal M, et al. "A Spatiotemporal Reconstruction of the C. elegans Pharyngeal Cuticle Reveals a Structure Rich in Phase-Separating Proteins." https://www.biorxiv.org/content/10.1101/2022.03.11.483951v1 |
| RP3498 | trEx1001. | C elegans | trEx1001 [idpb-3p::idpb-3::mNeonGreen + myo-2p::mCherry]. Pick animals with mCherry+ pharynx to maintain. Generated in N2 background. Reference: Kamal M, et al. bioRxiv 2022.03.11.483951; doi: https://doi.org/10.1101/2022.03.11.483951. |
| RP3510 | trEx1010. | C elegans | trEx1010 [pgp-14p::sms-5B(genomic)::Flag::mCherry + pgp-14p::YFP::pgp-14]. Pick animals with mCherry+ pharynx to maintain. Fluorescence is more apparent in older animals (late stage larvae to young adults). Generated in N2 background. Reference: Kamal M, et al. bioRxiv 2022.03.11.483951; doi: https://doi.org/10.1101/2022.03.11.483951. |
| RP3513 | trEx1006. | C elegans | trEx1006 [fipr-4p::fipr-4::mNeonGreen + myo-2p::mCherry]. Pick animals with mCherry+ pharynx to maintain. Generated in N2 background. Reference: Kamal M, et al. bioRxiv 2022.03.11.483951; doi: https://doi.org/10.1101/2022.03.11.483951. |
| RP3515 | trEx1008. | C elegans | trEx1008 [idpa-3p::idpa-3::mNeonGreen + myo-2p::mCherry]. Pick animals with mCherry+ pharynx to maintain. Generated in N2 background. Reference: Kamal M, et al. bioRxiv 2022.03.11.483951; doi: https://doi.org/10.1101/2022.03.11.483951. |
| RP582 | egl-19(tr69) IV. | C. elegans | Nemadipine-A resistant. |
| RP583 | egl-19(tr70) IV. | C. elegans | Mildly myotonic. Nemadipine-A resistant. |
| RP584 | egl-19(tr71) IV. | C. elegans | Nemadipine-A resistant. |
| RP585 | egl-19(tr72) IV. | C. elegans | Myotonic. Nemadipine-A resistant. |
| RP586 | egl-19(tr73) IV. | C. elegans | Mildly myotonic. Nemadipine-A resistant. |
| RP828 | madd-2(tr103) V. | C. elegans | Midline-oriented migration defects including muscle arm extension and HSN, AVM, and PVM axon guidance defects. Reference: Alexander M, et al. Dev Cell. 2010 Jun 15;18(6):961-72. |
| SD1075 | gaIs146. | C. elegans | gaIs146 [(pPRSK9) myo-3p::FLAG::pab-1 + sur-5::GFP]. Slightly slower growth than WT. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| tr129 | Allele | substitution | nonsense |
| tr69 | Allele | substitution | |
| tr70 | Allele | substitution | |
| tr71 | Allele | substitution | |
| tr72 | Allele | substitution | |
| tr73 | Allele | substitution | |
| tr103 | Allele | substitution | nonsense |