Laboratory Information
| Name | QP View on WormBase |
|---|---|
| Allele designation | ea |
| Head | Judith L Yanowitz |
| Institution | Magee-Womens Research Institute/U. Pittsburgh |
| Address | 204 Craft Avenue Pittsburgh 15213 United States |
| Website | http://yanoworm.org/ |
| Gene classes | xnd smrc |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| PD3011 | cyd-1(cc600) II; cup-5(ar465) III; arIs39 X. | C. elegans | arIs39 [myo-3p::ssGFP + dpy-20(+)]. |
| QP1208 | sws-1(ea12) V. | C. elegans | Increased lethality and male frequency. Synthetic lethal with helq-1(tm2134). Sensitive to camptothecin. Interacts with rip-1 and rfs-1. Reference: McClendon TB, et al. Genetics. 2016 May;203(1):133-45. |
| QP122 | dpy-3(e27) lon-2(e678) unc-3(e151) X. | C. elegans | Dpy. Unc. dpy-3 is epistatic to lon-2. |
| QP1398 | him-5(ea42) V. | C. elegans | CRISPR/Cas9-engineered deletion of him-5. High incidence of males. Reference: Macaisne N, et al. Genetics (2018) 2010:843-856. PMID 30242011 |
| QP1961 | eaIs4. | C. elegans | eaIs4 [him-5p::him-5::GFP::3xFLAG::him-5 3'UTR + unc-119(+)]. Transgene recapitulates published HIM-5 expression patterns and can rescue high incidence of males phenotype of him-5 mutants. Reference: McClendon TB. et al. G3 (Bethesda). 2016 Dec 7;6(12):3913-3925. PMID 27678523. |
| QP1962 | eaIs15 III. | C. elegans | eaIs15 [pie-1p::him-5::GFP::pie-1 3’ UTR + unc-119(+)] III. eaIs15 can rescue high incidence of males phenotype of him-5 mutants. Reference: McClendon TB. et al. G3 (Bethesda). 2016 Dec 7;6(12):3913-3925. PMID 27678523. |
| QP218 | unc-34(e315) dpy-11(e224) rol-9(sc148) V. | C. elegans | Unc. Dpy. Rol(ts). |
| QP220 | unc-60(m35) dpy-11(e224) rol-9(sc148) V. | C. elegans | Unc. Dpy. Rol(ts). |
| QP2460 | hIn1 [umnIs78 unc-54(h1040)]/+ I. | C. elegans | umnIs78 [myo-2p::mKate2 + NeoR, I: 12541645 (intergenic)] I. Heterozygous strain. Crossover suppressor for LGI right. Inversion includes unc-75 and unc-54(h1040). Heterozygotes are wild-type (not paralyzed) with dim mKate2 expression in pharynx, and segregate heterozygotes (not paralyzed, dim mKate2), homozygous wild-type (not paralyzed, no mKate2), and hIn1 homozygotes (paralyzed, bright mKate2). Pick non-paralyzed, dim mKate2 worms and check for correct segregation of progeny to maintain. Maintain at 20C or higher: recombined balancer seems more prone to breaking down at low temperatures. Derived by crossing parental strain CGC105 (hIn1[umnIs78]) to RG3173 (Y40B1B.7(ve673[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP])/hIn1[unc-54(h1040)]) and selecting for the recombined hIn1 worms. |
| QP2479 | hIn1 [umnIs75 unc-54(h1040)]/+ I. | C. elegans | umnIs75 [myo-2p::GFP + NeoR, I: 12541645 (intergenic)] I. Heterozygous strain. Crossover suppressor for LGI right. Inversion includes unc-75 and unc-54(h1040). Heterozygotes are wild-type (not paralyzed) with dim GFP expression in pharynx, and segregate heterozygotes (not paralyzed, dim GFP), homozygous wild-type (not paralyzed, no GFP), and hIn1 homozygotes (paralyzed, bright GFP). Pick non-paralyzed, dim GFP worms and check for correct segregation of progeny to maintain. Maintain at 20C or higher: recombined balancer seems more prone to breaking down at low temperatures. Derived cross of parental strains CGC94 (hIn1[umnIs75]) to QP2460 (hIn1[umnIs78, unc-54(h1040)]/+) and selecting for the recombined hIn1 worms. |
This laboratory hasn't submitted any alleles to the CGC.