Laboratory Information
| Name | OCF View on WormBase |
|---|---|
| Allele designation | ocf |
| Head | Orna Cohen-Fix |
| Institution | NIH, NIDDK, Bethesda, MD |
| Address | NIH, NIDDK, LCMB Building 8 Room 319 8 Center Dr Bethesda 20892 United States |
| Website | http://intramural.niddk.nih.gov/research/faculty.asp?People_ID=1564 |
| Gene classes | lpin |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| AG175 | unc-119(ed3) III; avEx122. | C. elegans | avEx122 [(pAG126) lpin-1p::GFP::lpin-1::lpin-1 3'UTR + unc-119(+)]. No GFP expression detected in germline. |
| AG176 | unc-119(ed3) III; avEx123. | C. elegans | avEx123 [lpin-1p::GFP::his-58::lpin-1 3'UTR + unc-119(+)]. No GFP expression detected in germline. |
| OCF108 | unc-119(ed3) III; atln-1(ocf102[atln-1::GFP::3xFLAG]) IV; ocfIs2. | C. elegans | ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP::3xFlag tag inserted at C-terminus of endogenous atln-1 locus. ocf102 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF116 | unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) IV; ojIs23. | C. elegans | ojIs23 [pie-1p::GFP::spcs-1 + unc-119(+)]. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF12 | lpin-1(ok2761) V/nT1 [qIs51] (IV;V). | C. elegans | Heterzygotes are wildtype and GFP+. lpin-1(ok2761) homozygotes die as L1 larvae. Homozygous nT1[qIs51] inviable. |
| OCF124 | unc-119(ed3) III; ItIs33; ocfIs2. | C. elegans | ItIs33 [pie-1p::GFP::TEV::stag::sas-6 + unc-119(+)]. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF127 | bsSi15 I; unc-119(ed3) III; ojIs23. | C. elegans | bsSi15 [spd-2p::spd-2::mCherry::spd-2 3'UTR + unc-119(+)] I. ojIs23 [pie-1p::GFP::spcs-1 + unc-119(+)]. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF13 | lpin-1(ok2761)/sqt-3(sc8) unc-61(e228) V; ltIs37 IV; jjIs1092. | C. elegans | jjIs1092 [(pNUT1) npp-1::GFP + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Heterozygotes are wildtype with GFP+ and mCherry+. lpin-1(ok2761) homozygotes die as L1 larvae. Also segregates Unc Rol. |
| OCF15 | unc-119(ed3); ocfIs2. | C. elegans | ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. Stable germline and embryonic expression of ER and NE marker, useful for following NE dynamics through early development. Reference: Joseph-Strauss D, et al. Dev Biol. 2012 May 15;365(2):445-57. |
| OCF158 | unc-119(ed3) III; ltIs78; ocfIs2. | C. elegans | ltIs78 [pie-1p::GFP::TEV::Stag::air-1 spliced coding + unc-119(+)]. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF162 | ebp-2(or1954[ebp-2::mKate2]) II; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ocfIs2. | C. elegans | ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mKate2 tag inserted into C-terminus of endogenous ebp2 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF164 | spd-5(vie26[GFP::spd-5::loxP]) I; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ocfIs2. | C. elegans | ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted into N-terminus of endogenous spd-5 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF165 | spd-5(vie26[GFP::spd-5::loxP]) I; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) IV; ocfIs2. | C. elegans | ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted into N-terminus of endogenous spd-5 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF167 | tac-1(or1955[GFP::tac-1]) II; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ocfIs2. | C. elegans | ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted into N-terminus of endogenous tac-1 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF172 | unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ltIs78; ocfIs2. | C. elegans | ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ltIs78 [pie-1p::GFP::TEV::Stag::air-1 spliced coding + unc-119(+)]. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF176 | spd-5(vie26[GFP::spd-5::loxP]) I; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2] III; ocfIs2. | C. elegans | ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted at N-terminus of endogenous spd-5 locus. mTurquoise2 tag with linker inserted at C-terminus of endogenous his-72 locus. mCherry::SP12 is a stable germline and embryonic expression of ER and NE marker. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF178 | bsSi15 I; unc-119(ed3) III; sun-1(ocf103[sun-1::GFP::3xFLAG]) V. | C. elegans | bsSi15 [spd-2p::spd-2::mCherry::spd-2 3'UTR + unc-119(+)] I. GFP::3xFlag tag inserted at C-terminus of endogenous sun-1 locus. ocf103 guide: GTTGGGCAGGAACAATAATA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF179 | npp-12(ax4539[npp-12::mNeonGreen]) bsSi15 I; unc-119(ed3) III. | C. elegans | bsSi15 [spd-2p::spd-2::mCherry::spd-2 3'UTR + unc-119(+)] I. mNeonGreen tag inserted at the C-terminus of the endogenous npp-12 locus. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF181 | unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2] III; ltIs25; ocfIs2. | C. elegans | ltIs25 [pie-1p::GFP::tba-2 + unc-119(+)]. ItIs25 is a translation fusion of GFP to unspliced genomic tba-2/C47B2.3. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mTurquoise2 tag with linker inserted at C-terminus of endogenous his-72 locus. mCherry::SP12 is a stable germline and embryonic expression of ER and NE marker. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF183 | unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2] III; ieSi38 IV; atln-1(ocf101[atln-1::3xFLAG::AID*]) IV; ltIs25; ocfIs2. | C. elegans | ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ltIs25 [pie-1p::GFP::tba-2 + unc-119(+)]. ItIs25 is a translation fusion of GFP to unspliced genomic tba-2/C47B2.3. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mTurquoise2 tag with linker inserted at C-terminus of endogenous his-72 locus. 3xFLAG::AID* tag inserted at C-terminus of endogenous atln-1 locus. mCherry::SP12 is a stable germline and embryonic expression of ER and NE marker. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370. |
| OCF22 | unc-119(ed3) III; ocfIs5. | C. elegans | ocfIs5 [pie-1p::mCherry::npp-1::pie-1 3'UTR + unc119(+)]. Reference: Joseph-Strauss D, et al. Dev Biol. 2012 May 15;365(2):445-57. |
| OCF3 | unc-119(ed3) III; ltIs37 IV; jjIs1092. | C. elegans | jjIs1092 [(pNUT1) npp-1::GFP + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Non-Unc. |
| OCF4 | unc-119(ed3) III; ltIs37 IV; qaIs3502. | C. elegans | qaIs3502 [YFP::lmn-1 + CFP::H2B + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Non-Unc. |
| OCF69 | ocfSi1 I; unc-119(ed3) III. | C. elegans | ocfSi1 [mex-5p::Dendra2::his-58/66::tbb-2 3'UTR + unc-119(+)] I. Dendra2::histone H2B expression in the germline and embryos. The H2B coding sequence is identical to genes his-58 and his-66. Made by MosSCI insertion into ttTi4348 on LG I. Reference: Rosu S & Cohen-Fix O. Dev Biol. 2017 Mar 15;423(2):93-100. |
This laboratory hasn't submitted any alleles to the CGC.