Laboratory Information
| Name | OC View on WormBase |
|---|---|
| Allele designation | bs |
| Head | O'Connell, Kevin |
| Institution | NIDDK/NIH, Bethesda, MD |
| Address | NIH 9000 Rockville Pike Bethesda 20892 United States |
| Website | http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/OConnellKevin.htm |
| Gene classes | szy sds |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| OC100 | zyg-1(it25) II; sun-1(bs12) szy-18(b53) V. | C. elegans | bs12 and bs53 partially suppress zyg-1. Grow at 20C. |
| OC133 | szy-20(bs52) II. | C. elegans | Temperature-sensitive maternal effect lethal; 99% of embryos fail to hatch at 25 C. Maintain at 15-20 C. Reference: Song et al. (2008) Dev Cell 15:901-12. |
| OC188 | zyg-1(it25) szy-16(bs36) II. | C. elegans | it24 is temperature sensitive; produces maternal effect embryonic lethal phenotype (Mel) after shoft to 24C at L4 stage. Mel is partially suppressed by bs36 at 24C. Reference: Kemp C, et al. (2007) Genetics 176:95-113. |
| OC199 | zyg-1(it25) sds-22(bs9) II. | C. elegans | it24 is temperature sensitive; produces maternal effect embryonic lethal phenotype (Mel) after shoft to 24C at L4 stage. Mel is partially suppressed by bs9 at 24C. sds-22 previously called szy-6. Reference: Kemp C, et al. (2007) Genetics 176:95-113. |
| OC202 | zyg-1(it25) II; szy-12(bs16) V. | C. elegans | zyg-1(it25) EMB partially suppressed at 24C. |
| OC204 | zyg-1(it25) dpl-1(bs21) II. | C. elegans | it24 is temperature sensitive; produces maternal effect embryonic lethal phenotype (Mel) after shoft to 24C at L4 stage. Mel is partially suppressed by bs21 at 24C. szy-10(bs21) has a partially penetrant embryonic lethal phenotype at all temperatures. Reference: Kemp C, et al. (2007) Genetics 176:95-113. |
| OC221 | szy-5(bs7) I. | C. elegans | Temperature-sensitive maternal effect lethal; ~50% of embryos fail to hatch at 25 C. Maintain at 15-20 C. Reference: Kemp et al. (2007) Genetics 176:95-113. |
| OC235 | sun-1(bs12) unc-76(e911) V. | C. elegans | Unc. bs12 mutation causes sublethal defect in attachment of centrosome to the nucleus in early embryos. Viable 15-25 C. Reference: Kemp et al. (2007) Genetics 176:95-113. |
| OC248 | zyg-1(it25) II; szy-11(bs22) V. | C. elegans | Maternal-effect embryonic lethal (L4 shift up) of zyg-1(it25) partially suppressed by szy-11(bs22). |
| OC271 | pph-4.1(tm1598) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Homozygous sterile mutation balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm1598 homozygotes (produce ~100% dead eggs, slightly higher penetrance at 25C). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Peters N, et al., J Cell Sci. 2010 Mar 1;123(Pt 5):795-805. |
| OC95 | bsIs2 ruIs32 III. | C. elegans | bsIs2 [pie-1::GFP::spd-2 + unc-119(+)]. ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Wild type animals express GFP marked DNA (GFP-H2b) and centrosomes (GFP-SPD-2) in germ line and embyros. Maintain at 24C to obtain optimal GFP expression. |
| TH26 | unc-119(ed3) III; ddEx10. | C. elegans | ddEx10 [pie-1p::GFP::sas-4 + unc-119(+)]. Maintain by picking non-Unc. |
| TH30 | unc-119(ed3) ruIs32 III; ddIs6 V. | C. elegans | ddIs6 [tbg-1::GFP + unc-119(+)]. ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. unc-119(ed3) mutation might have been lost during crossing of TH27 and AZ212. |
| TH61 | unc-119(ed3) III; ddIs36. | C. elegans | ddIs36 [pie-1p::GFP::sas-5 + unc-119(+)]. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| bs137 | Allele | ||
| bs12 | |||
| bs52 | Allele | ||
| bs36 | Allele | ||
| bs9 | Allele | ||
| bs16 | Allele | ||
| bs21 | Allele | ||
| bs7 | |||
| bs22 | Allele |