Laboratory Information

NameOC View on WormBase
Allele designationbs
HeadO'Connell, Kevin
InstitutionNIDDK/NIH, Bethesda, MD
Address NIH
9000 Rockville Pike
Bethesda 20892
United States
Website http://www2.niddk.nih.gov/NIDDKLabs/IntramuralFaculty/OConnellKevin.htm
Gene classes szy  sds 

Strains contributed by this laboratory

Strain Genotype Species Description
OC100 zyg-1(it25) II; sun-1(bs12) szy-18(b53) V. C. elegans bs12 and bs53 partially suppress zyg-1. Grow at 20C.
OC133 szy-20(bs52) II. C. elegans Temperature-sensitive maternal effect lethal; 99% of embryos fail to hatch at 25 C. Maintain at 15-20 C. Reference: Song et al. (2008) Dev Cell 15:901-12.
OC188 zyg-1(it25) szy-16(bs36) II. C. elegans it24 is temperature sensitive; produces maternal effect embryonic lethal phenotype (Mel) after shoft to 24C at L4 stage. Mel is partially suppressed by bs36 at 24C. Reference: Kemp C, et al. (2007) Genetics 176:95-113.
OC199 zyg-1(it25) sds-22(bs9) II. C. elegans it24 is temperature sensitive; produces maternal effect embryonic lethal phenotype (Mel) after shoft to 24C at L4 stage. Mel is partially suppressed by bs9 at 24C. sds-22 previously called szy-6. Reference: Kemp C, et al. (2007) Genetics 176:95-113.
OC202 zyg-1(it25) II; szy-12(bs16) V. C. elegans zyg-1(it25) EMB partially suppressed at 24C.
OC204 zyg-1(it25) dpl-1(bs21) II. C. elegans it24 is temperature sensitive; produces maternal effect embryonic lethal phenotype (Mel) after shoft to 24C at L4 stage. Mel is partially suppressed by bs21 at 24C. szy-10(bs21) has a partially penetrant embryonic lethal phenotype at all temperatures. Reference: Kemp C, et al. (2007) Genetics 176:95-113.
OC221 szy-5(bs7) I. C. elegans Temperature-sensitive maternal effect lethal; ~50% of embryos fail to hatch at 25 C. Maintain at 15-20 C. Reference: Kemp et al. (2007) Genetics 176:95-113.
OC235 sun-1(bs12) unc-76(e911) V. C. elegans Unc. bs12 mutation causes sublethal defect in attachment of centrosome to the nucleus in early embryos. Viable 15-25 C. Reference: Kemp et al. (2007) Genetics 176:95-113.
OC248 zyg-1(it25) II; szy-11(bs22) V. C. elegans Maternal-effect embryonic lethal (L4 shift up) of zyg-1(it25) partially suppressed by szy-11(bs22).
OC271 pph-4.1(tm1598) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Homozygous sterile mutation balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm1598 homozygotes (produce ~100% dead eggs, slightly higher penetrance at 25C). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Peters N, et al., J Cell Sci. 2010 Mar 1;123(Pt 5):795-805.
OC95 bsIs2 ruIs32 III. C. elegans bsIs2 [pie-1::GFP::spd-2 + unc-119(+)]. ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Wild type animals express GFP marked DNA (GFP-H2b) and centrosomes (GFP-SPD-2) in germ line and embyros. Maintain at 24C to obtain optimal GFP expression.
TH26 unc-119(ed3) III; ddEx10. C. elegans ddEx10 [pie-1p::GFP::sas-4 + unc-119(+)]. Maintain by picking non-Unc.
TH30 unc-119(ed3) ruIs32 III; ddIs6 V. C. elegans ddIs6 [tbg-1::GFP + unc-119(+)]. ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. unc-119(ed3) mutation might have been lost during crossing of TH27 and AZ212.
TH61 unc-119(ed3) III; ddIs36. C. elegans ddIs36 [pie-1p::GFP::sas-5 + unc-119(+)].

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
bs137 Allele
bs12
bs52 Allele
bs36 Allele
bs9 Allele
bs16 Allele
bs21 Allele
bs7
bs22 Allele