Laboratory Information

NameNW View on WormBase
Allele designationev
HeadJoseph Gary Culotti
InstitutionMt. Sinai Hospital Research Institute, Toronto, Ontario
Address 600 University Ave
Toronto M5G1X5
Canada
Website http://www.mshri.on.ca/culotti/DEFAULT.ASP
Gene classes avm  efn  erf  eva  seu  smp  stau 

Strains contributed by this laboratory

Strain Genotype Species Description
DG2361 vab-2(e961) efn-2(ev658) IV; efn-3(ev696) X. C. elegans
GG201 ace-2(g72) I; ace-1(p1000) X. C. elegans Slow moving Unc. Cannot back. Hypercontracts when you tap nose.
GG202 ace-2(g72) I. C. elegans Phenotypically WT. AChE ACTIVITY :SENS TO 0.1% TRITON X-100.
NW1255 seu-1(ev572) IV. C. elegans Suppresses touch receptor axon guidance defect of mec-7::unc-5.
NW1313 mig-6(ev700) V. C. elegans Abnormal distal tip cell migration detectable as clear patches in anterior and posterior. Partially penetrant embryonic arrest and early larval lethal.
NW1314 mig-6(ev701) V. C. elegans Abnormal distal tip cell migration detectable as clear patches in anterior and posterior. Partially penetrant embryonic arrest and early larval lethal.
NW1549 efn-2(ev658) efn-3(ev696) C. elegans Vab, embryonic ventral enclosure defects, male ray fusions.
NW1550 efn-2(ev658) him-5(e1490) C. elegans Male ray fusions: approx 15% penetrant.
NW1613 msh-2(ev679::Tc1) I. C. elegans Reduced fertility. Reduced long-term survival. Mutator phenotype. DNA damage-induced apoptosis in the germ line. No effect on lifespan or meiotic chromosome disjunction observed.
NW1615 plx-1(ev724) jcIs1 IV; him-5(e1490) V. C. elegans jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
NW1619 dpy-11(e224) mig-6(ev700) V/eT1 (III;V). C. elegans Heterozygotes display abnormal distal tip cell migration detectable as clear patches in anterior and posterior. Heterozygotes segregate WT, Dpy, abnormal DTC migration, Unc, and dead eggs. Maintain by picking wild-type.
NW1623 dpy-11(e224) mig-6(ev788) V/eT1 (III;V). C. elegans Heterozygotes display partially penetrant (~10%) abnormal distal tip cell migration detectable as clear patches in anterior and posterior. Heterozygotes segregate WT, abnormal DTC migration, Unc, and dead eggs. Maintain by picking wild-type.
NW1625 dpy-11(e224) mig-6(e1931) V/eT1 (III;V). C. elegans Heterozygotes are wild-type and segregate WT, Dpy-Ste Unc, and dead eggs. Maintain by picking wild-type.
NW1644 evEx170. C. elegans evEx170 [smp-1::GFP(pVGS1a::GFP) + rol-6(su1006)]. Maintain by picking Rollers. GFP expression in muscle, neuron, vuvlal cells and male ray cells.
NW1675 mig-6(oz113) V/nT1 [qIs51] (IV;V). C. elegans Heterozygotes are wild-type and GFP+. Heterozygotes segregate WT GFP+, sterile GFP- homozygotes, and dead eggs. Maintain by picking GFP+ wild-type.
NW1692 him-5 V; evIs190. C. elegans evIs190 [vab-1p::Venus + rol-6(su1006)]. Rollers. Reference: Ikegami et al. Curr Biol. 2012 Jan 10;22(1):1-11.
NW1696 him-5(e1490) V; evIs138. C. elegans evIs138 [plx-2(+) + sur-5::GFP]. Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1697 plx-2(ev775) II; him-5(e1490) V. C. elegans Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1698 plx-2(ev774) II; him-5(e1490) V. C. elegans Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1699 plx-2(ev738) II; him-5(e1490) V. C. elegans Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single.
NW1700 plx-2(ev773) II; him-5(e1490) V. C. elegans Null allele. Enhances sensory ray fusion defect of mab-20(bx61). No fusions as single. Some embryonic and larval lethality.
NW1701 mab-20(ev778) I; muIs32 II; him-5(e1490) V. C. elegans muIs32 [mec-7p::GFP + lin-15(+)]. Extensive ray fusion. Body morphology defects at larval stages. Embryonic and larval lethality.
NW1702 smp-1(ev715) I; jcIs1 IV; him-5(e1490) V. C. elegans jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Strain does not Roll but otherwise seems fine. Male ray 1 anterior displacement in homozygous animals. Genetic interaction with smp-2(ev709). Vulva cell migration defects. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
NW1703 him-5(e1490) V; evEx193. C. elegans evEx193 [semala::GFP]. GFP fluorescence in interneurons (AVL), the excretory channel, the distal tip cells (DTC), somatic cells of the gonad, epidermal cells. In adult, expression in the fused seam cell syncitium.
NW1704 smp-2(ev709) I; jcIs1 IV; him-5(e1490) V. C. elegans jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Strain does not Roll but otherwise seems fine. Male ray 1 anterior displacement in homozygous animals. Genetic interaction with smp-1(ev715). ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Koppen M, et al. Nat Cell Biol. 2001 Nov;3(11):983-91.
NW411 enu-1(ev419) II; vab-8(ev411) V. C. elegans ev411 was previously called unc-107.
NW427 vab-8(ev411) V. C. elegans Slightly Unc. ev411 was previously called unc-107.
NW434 unc-6(ev400) X. C. elegans Unc.
NW906 pag-1(ls2) III; dpy-20(e1282) IV; seu-2(ev523) evIs41 V. C. elegans evIs41[mec-7::lacZ; mec-7::unc-5; dpy-20(+)] V. Suppresses axon guidance phenotype ofmec-7::unc-5. Has slight Uncoordinated phenotype.
NW987 unc-129(ev554) IV. C. elegans Unc. Kinker, especially in backward movement.
NW988 pag-1(ls2) III; dpy-20(e1282) IV; seu-3(ev555) evIs41 V. C. elegans evIs41[mec-7::lacZ; mec-7::unc-5 + dpy-20(+)] V. Suppresses axon guidance phenotype ofmec-7::unc-5. Has slight Uncoordinated phenotype.
NW990 unc-129(ev557) IV. C. elegans Unc. Kinker, especially in backward movement.
NW999 unc-129(ev566) IV. C. elegans Unc. Kinker, especially in backward movement.
PR801 che-3(p801) I. C. elegans Fails to avoid high osmotic strength solutions of NaCl and fructose. Fails to stain amphids with FITC.
PR802 osm-3(p802) IV. C. elegans Fails to avoid high osmotic strength solutions of NaCl and fructose. Fails to stain amphids with FITC.
PR811 osm-6(p811) V. C. elegans Fails to avoid high osmotic strength solutions of NaCl and fructose. Fails to stain amphids with FITC.
PR813 osm-5(p813) X. C. elegans Fails to avoid high osmotic strength solutions of NaCl and fructose. Fails to stain amphid neurons with FITC.
PR821 daf-10(p821) IV. C. elegans Fails to avoid high osmotic strength solutions of NaCl and fructose. Fails to stain amphid neurons with FITC.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
ev740 Allele substitution splice_site
ev741 Allele
ev658 Allele deletion
ev696 Allele deletion
ev802 Allele deletion
ev572 Allele
ev686 Allele insertion frameshift
ev700 Allele substitution
ev701 Allele substitution
ev724 Allele deletion
ev788 Allele deletion
ev775 Allele substitution nonsense
ev774 Allele deletion
ev738 Allele
ev773 Allele deletion
ev778 Allele
ev715 Allele deletion
ev709 Allele deletion
ev419 Allele
ev411 Allele
ev424 Allele insertion frameshift
ev400 Allele
ev523 Allele
ev554 Allele substitution
ev555 Allele
ev557 Allele substitution nonsense
ev566 Allele substitution
ev571 Allele insertion