Laboratory Information
Name | NC View on WormBase |
---|---|
Allele designation | wd |
Head | David Milton Miller |
Institution | Vanderbilt University, Nashville, TN |
Address | Cell and Developmental Biology 3114 MRB3 BioSciences Building 465 21st Avenue South Nashville 37240 United States |
Website | http://exploration.vanderbilt.edu/news/news_worm.htm |
Gene classes | blr nab |
Strains contributed by this laboratory
Strain | Genotype | Species | Description |
---|---|---|---|
CB4330 | unc-4(e2309) II. | C. elegans | Reference: |
LX837 | vsIs45. | C. elegans | vsIs45 [tph-1::GFP]. tph-1::GFP in vsIs45 is expressed exclusively in the NSM neurons in larvae and NSM + HSN in adults, whereas other tph-1::GFP reporters are also expressed in other serotonergic neurons; can be used to image NSM development and for FACS isolation of NSM from L1s. Reference: Nelson JC, Colon-Ramos DA. J Neurosci. 2013 Jan 23;33(4):1366-76. |
NC1013 | unc-119(ed3) III; wdEx455. | C. elegans | wdEx455 [acr-14::GFP + unc-119(+)]. Pick non-Unc to maintain. |
NC1015 | unc-119(ed3) III; wdEx457. | C. elegans | wdEx457 [F39B2.8::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in pharyngeal muscles, posterior ventral cord motor neurons, tail neurons, and a few head neurons. Construct made by M. Vidal lab; candidate unc-37 target gene. F39B2.8 also known as gpr-158. |
NC106 | unc-37(e262 wd26) I. | C. elegans | Incompletely-penetrant exploding vulva. wd26 (E580K) is an intragenic revertant of the e262 allele for the backward movement phenotype; it is not known if it also modifies other e262 phenotypes. The wd26 lesion (E580K) is molecularly identical to wd14, wd16, wd17, wd18, etc. Reference: Pflugrad A, et al. Development. 1997 May;124(9):1699-709. |
NC138 | dpy-20(e1282) IV; wdIs3 X. | C. elegans | wdIs3[del-1::GFP + dpy-20(+)]. del-1 is expressed in the VB motor neurons beginning the the L2 larval stage. By the end of L2, del-1::GFP is also visible in a few VA motor neurons at the anterior end of the nerve cord. Expression of del-1::GFP in the VAs progresses in a wave from anterior to posterior, with all VAs expressing del-1::GFP by the adult stage. Thus, del-1::GFP is not expressed in the VAs during the L2 period in which unc-4 functions in those cells to establish synaptic inputs but is expressed in the VAs after they have been wired into the ventral cord circuit. del-1::GFP is also expressed in five neurons (VB1, VB2, SABVR, SABVL, VA1) in the retrovesicular ganglion at the anterior end of the ventral nerve cord. During the mid-L2 larval stage, del-1::GFP expression in the ventral nerve cord is largely restricted to the VB class of motor neurons. |
NC1469 | unc-119(ed3) III; wdEx575. | C. elegans | wdEx575 [ZC155.2::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expressed in cholinergic motor neurons, head & tail , neurons, and excretory cell. Construct made by Marc Vidal's group at Harvard as part of the promoterome project. |
NC1528 | unc-119(ed3) III; wdEx595. | C. elegans | wdEx595 [F08G12.1::GFP + unc-119(+)]. Pick non-Unc to maintain. Construct made by Marc Vidal's group at Harvard as part of the promoterome project. |
NC1624 | unc-119(ed3) wdIs62 III. | C. elegans | wdIs62 contains [ceh-12::GFP + unc-119(+)]. Superficially wild-type. |
NC168 | unc-4(e26) II; dpy-20 IV; wdEx60. | C. elegans | wdEx60 contains [acr-5::GFP + dpy-20(+)]. Maintain by picking non-Dpy. |
NC1686 | wdIs51. | C. elegans | wdIs51 [F49H12.4::GFP + unc-119(+)]; likely integrated in X. GFP expression in PVD. |
NC1687 | wdIs52. | C. elegans | wdIs52 [F49H12.4::GFP + unc-119(+)]. |
NC1700 | unc-119(ed3) III; wdEx637. | C. elegans | wdEx637 [dat-1::3xFLAG::pab-1 + unc-119(+)]. Pick non-Unc to maintain. Reference: Spencer WC, et al. Genome Res. 2011 Feb;21(2):325-41. |
NC1730 | unc-5(e152) IV; wdIs52. | C. elegans | wdIs52 [F49H12.4::GFP + unc-119(+)]. PVD defects in primary branch guidance, number of secondary branches, and tertiary branches are longer than wild-type. |
NC1749 | pha-1(e2123) hdIs32 III; otEx239. | C. elegans | hdIs32 [glr-1::DsRed2] III. otEx239 [rig-3::GFP + pha-1(+)]. Maintain at 25C and pick GFP+ to ensure the array is retained. AVA neurons are marked with GFP and DsRed2. Can be used to isolate AVA by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). Derived from parental strain OH4326. Reference: Hutter H. J Microsc. 2004 Aug;215(Pt 2):213-8. doi: 10.1111/j.0022-2720.2004.01367.x. PMID: 15315508. |
NC1750 | hdIs32 III; gvEx173. | C. elegans | hdIs32 [glr-1::DsRed2]. gvEx173 [opt-3::GFP + rol-6(su1006)]. Maintain by picking Rollers, which should be GFP+. hdIs32 might be prone to silencing. |
NC190 | wdIs6 II; dpy-20(e1282) IV. | C. elegans | wdIs6 [del-1::GFP + dpy-20(+)]. Embryonic GFP expression in SABVR, SABVL, VBs in mid-L2, VAs in mid-L3. |
NC197 | wdIs4 II; dpy-20(e1282) IV. | C. elegans | wdIs4 [unc-4::GFP + dpy-20(+)] II. Slightly Unc. GFP expression mosaic, occasional DA axon guidance defects. Embryonic expression: I5, DA, SABS; L1: AVF, VA; Late L3: VC. Early L1: GFP bright in I5, SABs, DA8/9, dim in DA1. |
NC216 | lin-15B&lin-15A(n765); wdEx75. | C. elegans | wdEx75 [acr-5::GFP + lin-15(+)]. Expressed in B-type motor neurons. |
NC2484 | wdIs5 III; dpy-20(e1282) IV. | C. elegans | wdIs5 [unc-4::GFP + dpy-20(+)] III. Reference: Lickteig KM, et al. J Neurosci. 2001 Mar 15;21(6):2001-14. |
NC2537 | unc-119(ed3) III; wdEx848. | C. elegans | wdEx848 [mec-4::mCherry + unc-119(+)]. Pick non-Unc to maintain. mCherry expression in all six touch neurons. Array generated by bombardment; ~90% transmission rate. Reference: Smith CJ, O'Brien T, et al. Neuron. 2013 July 24; 79. |
NC268 | unc-4(wd44) II. | C. elegans | Unc. Reference: Winnier AR, et al. Genes Dev. 1999 Nov 1;13(21):2774-86. |
NC279 | del-1(ok150) X. | C. elegans | 2 kb deletion mutant made by OMRF Knockout Group. Presumptive null. No obvious phenotype. Primers used: EL1: GAAACGGTGAGTGCCAATTT. ER1: AGTGCTGTCACACCAAGCAC. IL1: AAACCAACTGACCCAAGGTG. IR1: TATCTAGGGTCCGCACAACC. Left breakpoint sequence: AGGTTGACAAATTGTTGCGA. Right breakpoint sequence: CGCTTATTAAAAAATAATAT. |
NC2913 | hdIs1 X; ufIs26. | C. elegans | hdIs1 [unc-53p::GFP + rol-6(su1006)] X. ufIs26 [unc-4p::mCherry + lin-15(+)]. Rollers. DA neurons marked with unc-4::mCherry and unc-53::GFP. unc-53::GFP expression in DAs is dim during L1. Can be used to isolate DA neurons by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
NC292 | acr-5(ok182) III. | C. elegans | No obvious phenotype. 1.5 kb deletion of acr-5 produced by Moulder/Barstead at OMRF. Left breakpoint sequence: TGGGTGATGCTATATGCACA. Right breakpoint sequence: TAGACTTCCGAGCAATAATTC. |
NC293 | acr-5(ok180) III. | C. elegans | No obvious phenotype. 2 kb deletion of acr-5 produced by Moulder/Barstead at OMRF. Deletion removes all 4 transmembrane domains. This is likely a null allele. Left breakpoint sequence (includes repeated sequence): TTTTTAATTATCCGTAATTTTTTAATTATCCGTAAT. Right breakpoint sequence: AACATCTTTAATCGATTTAT. |
NC300 | dpy-20(e1282) IV; wdIs5. | C. elegans | wdIs5 [unc-4p::~1.5 exons of the unc-4 gene::GFP + (pMH86) dpy-20(+)]. Slightly Unc. GFP expression mosaic, occasional DA axon guidance defects. Embryonic expression: I5, DA, SABs; L1: AVF, VA; late L3: VC. |
NC3182 | otIs181 III; otIs138 X; otIs396. | C. elegans | otIs181[dat-1::mCherry + ttx-3::mCherry] III. otIs138[ser-2(prom3)::GFP + rol-6(su1006)] X. otIs396 [ace-1prom2::NLS::tagRFP]. Rollers. dat-1::mCherry labels ADE, CEP, and PDE neurons. ttx3::mCherry labels AIY neurons. ser-2(prom3)::GFP labels OLL, PDE, and PVD neurons. ace-1prom2::NLS::tagRFP labels CEP and OLL neurons. PVD can be identified by expression only GFP. An additional pair of GFP-only cells anterior to OLL are occasionally observed in this strain. Can be used to isolate PVD by FACS (green-only). Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). Reference: Barbara O’Brien (2017) Diverse genetic and transcriptional programs mediate dendritic development of a nociceptor neuron. Ph.D Dissertation, Vanderbilt University. (https://ir.vanderbilt.edu/handle/1803/14489?show=full) |
NC3292 | oig-1(wd114[oig-1::gfp11x7]) III. | C. elegans | Superficially wild-type. wd114 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous oig-1 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395. |
NC3296 | ynIs37 III; juIs223 IV. | C. elegans | ynIs37 [flp-13::GFP] III. juIs223 [ttr-39p::mCherry + ttx-3p::GFP] IV. ttr-39::mCherry alone marks VD and DD neurons. ttx-3::GFP marks AIY neurons. Combined ttr-39::mCherry and flp-13::GFP co-expression marks DD neurons in the L2 (only a few mCherry+/GFP+ DD neurons will show up briefly in the L2 stage and gene silencing apparently dims the GFP marker over time). VD neurons can be identified by mCherry alone, no GFP. Derived by crossing parental strains CZ8332 and NY2037. Can be used to isolate VB neurons by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
NC3381 | lev-10(wd116[lev-10::gfp11x7]) I. | C. elegans | wd116 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous lev-10 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395. |
NC3390 | oig-1(wd114[oig-1::gfp11x7]) III; wdEx1034. | C. elegans | wdEx1034 [rab-3p::GFP1-10 + myo-2p::mCherry]. Pick mCherry+ to maintain. wd114 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous oig-1 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395. |
NC3492 | lev-10(wd116[lev-10::gfp11x7]) I; wdEx1086. | C. elegans | wdEx1086 [myo-3p::GFP1-10 + myo-2p::mCherry]. Pick mCherry+ to maintain. wd116 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous lev-10 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395. |
NC3493 | lev-10(wd116[lev-10::gfp11x7]) I; wdEx1087. | C. elegans | wdEx1087 [ttr-39p::GFP1-10 + myo-2p::mCherry]. Pick mCherry+ to maintain. wd116 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous lev-10 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395. |
NC3524 | ufIs26 II; vsIs13 IV. | C. elegans | ufIs26 [unc-4p::mCherry + lin-15(+)] II. vsIs13 [lin-11::pes-10::GFP + lin-15(+)] IV. GFP expression in six VC neurons and posterior intestine. VC neurons are labeled with both GFP and mCherry; co-labeling in VCs1-5 is brightest during L4 stage. Derived by crossing parental strains NC2957 and LX959. Can be used to isolate VC neurons by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
NC3540 | otIs458. | C. elegans | otIs458[ceh-63p::GFP + unc-119(+)]. GFP expression in DVC neurons. Can be used to isolate DVC by FACS. Derived by outcrossing parental strain OH11974 to remove him-5(e1490). Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
NC3577 | ufIs26 II; otIs707. | C. elegans | ufIs26 [unc-4p::mCherry + lin-15(+)] II. otIs707 [bnc-1p(1.8kb)::GFP]. VB neurons are GFP+ only; VA and SABV have both mCherry and GFP. Can be used to isolate VB neurons by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
NC3604 | myls13; oyIs51. | C. elegans | myls13 [klp-6p::GFP]. oyIs51 [srh-142::RFP]. IL2 neurons are marked with GFP. ADF neurons are marked with RFP. Derived from parental line PY3470. Can be used to isolate IL2 neurons (GFP) and ADF neurons (RFP) by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
NC37 | unc-37(wd17) I; unc-4(e2322) II. | C. elegans | unc-37(wd17) is a dominat suppressor of unc-4(e2322ts). unc-37(wd17) has no phenotype alone. |
NC3738 | wdIs132; uIs152. | C. elegans | wdIs132 [gcy-35::GFP]. uIs152 [mec-3::RFP]. AVM neurons are labeled with GFP and RFP. Can be used to isolate AVM by FACS. wdIs132 arose from spontaneous integration of kyEx1162 [gcy-35::GFP]. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
NC3792 | ynIs37 III; ufIs26. | C. elegans | ynIs37 [flp-13p::GFP] III. ufIs26 [unc-4p::mCherry + lin-15(+)]. I5 neurons are labeled with GFP and RFP. Can be used to isolate I5 by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
NC4059 | pha-1(e2123) III; wdEx1201. | C. elegans | wdEx1201 [spe-27p::tagRFP + pha-1(+)]. Maintain at 25C to select for array. RMF neurons are labeled with TagRFP. Can be used to isolate RMF by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
NC4085 | otIs355 IV; otIs846. | C. elegans | otIs355 [rab-3p(prom1)::2xNLS::TagRFP] IV. otIs846 [egas-1p::GFP + unc-122p::GFP]. Pan-neuronal nuclear RFP expression. Co-expression of GFP and RFP in IL2 neuron can be used to isolate IL2 by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
NC467 | acr-5(ok205) III. | C. elegans | No obvious phenotype. 2.4 kb deletion of acr-5 produced by Moulder/Barstead at OMRF. |
NC571 | dpy-20 (e1282) IV; wdIs20. | C. elegans | wdIs20 [unc-4p::snb-1::GFP + dpy-20(+)]. Animals are Lon, likely due to dpy-20(+) overexpression. Punctate GFP expression observed in dorsal nerve cord, ventral nerve cord, VC4, and VC5. Reference: Lickteig KM, et al. J Neurosci. 2001 Mar 15;21(6):2001-14. |
NC574 | wdIs22. | C. elegans | wdIs22 [acr-5p::YFP + rol-6(su1006)]. Integrated array derived from wdEx223. Maintain by picking Rollers. |
NC694 | unc-119(ed1) III; wdEx257. | C. elegans | wdEx257 [unc-4::3XFLAG::pab-1 + (pSV17) unc-119 minigene]. Animals exhibit slight forward movement defect. Good antibody staining witih anit-FLAG M2 antibody (Sigma). 100% penetrant array. Expressed in all unc-4 neurons. Ectopically expressed in at least one head neuron (dorsal to pharynx, between two bulbs). |
NC698 | wdEx258. | C. elegans | wdEx258 [twk-30::GFP + myo-3::DsRed2)]. GFP expression in perinuclear motor neurons. |
NC782 | unc-119(ed3) III; wdEx290. | C. elegans | wdEx290 [acr-15::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene. |
NC821 | unc-119(ed3) III; wdEx329. | C. elegans | wdEx329 [F09C3.2::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in ventral nerve cord. Construct made by M. Vidal lab; candidate unc-37 target gene. |
NC844 | unc-119(ed3) III; wdEx345. | C. elegans | wdEx345 [F55C12.4::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene. |
NC845 | unc-119(ed3) III; wdEx346. | C. elegans | wdEx346 [tig-1::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in head neurons, tail neurons. head muscles, and vulva. Construct made by M. Vidal lab; candidate unc-37 target gene. |
NC847 | unc-119(ed3) III; wdEx348. | C. elegans | wdEx348 [C13G3.1::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene. |
NC85 | unc-37(wd17wd22)/dpy-14(e188) I. | C. elegans | Heterozygotes are WT and segregate WT, Dpys and Steriles. |
NC850 | unc-119(ed3) III; wdEx351. | C. elegans | wdEx351 [tsp-7::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in motor neurons. Construct made by M. Vidal lab; candidate unc-37 target gene. |
NC852 | unc-119(ed3) III; wdEx353. | C. elegans | wdEx353 [Y34D9B.1a::GFP + unc-119(+)]. mig-1::GFP construct made by Marc Vidal's group at Harvard as part of the promoterome project; unc-37 target gene. GFP expression observed in all classes of VNC motor neurons, head & tail neurons, body wall muscle, and intestine. [The strain is described as unc-119 and unc-119(+) as the co-injection marker, but looks to actually be lin-15 (Muv).] |
NC865 | unc-119(ed3) III; wdEx359. | C. elegans | wdEx359 [F29G6.2::GFP + unc-119(+)]. GFP+ seen in ventral nerve cord. Pick non-Unc worms that are GFP+. |
NC902 | unc-119(ed3) III; wdEx381. | C. elegans | wdEx381 [F55C12.4::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene. |
NC92 | unc-37(wd17wd20)/dpy-14(e188) I. | C. elegans | Heterozygotes are WT and segregate WT, Dpys, and Steriles. |
NC93 | unc-37(wd17wd21)/dpy-14(e188) I. | C. elegans | Heterozygotes are WT and segregate WT, Dpys and Steriles. |
NC972 | wdEx419. | C. elegans | wdEx419 [acr-16::GP + rol-6(su1006)]. Rollers. Maintain by picking rollers. GFP expression detectable in body wall muscle and DB motor neurons. |
NC987 | wdEx445. | C. elegans | wdEx445 [T27E9.9::GFP + rol-6(su1006))]. Pick rollers to maintain. GFP expression in DA and DB neurons during L1 stage, low levels of expression in VA neurons in later stages. |
NC996 | wdEx451. | C. elegans | wdEx451 [Y71D11A.5::GFP + rol-6(su1006))]. Pick rollers to maintain. GFP expression in VD, DD, and AS neurons, multiple head and tail neurons, and muscles. |
OH12503 | otIs520 X. | C. elegans | otIs520 [eat-4(prom11)::GFP + ttx-3::mCherry] X. AIM is marked only with GFP, not mCherry. Can be used to isolate AIM by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH15507 | otIs439; hdIs32. | C. elegans | otIs439 [lad-2p::GFP + pha-1(+)]. hdIs32 [glr-1::DsRed2]. SMD neurons are labeled with GFP and DsRed2. Can be used to isolate SMD by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH15623 | otIs706. | C. elegans | otIs706 [nlp-12p::TagRFP]. DVA neurons are labeled with TagRFP. Can be used to isolate DVA by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH16052 | otIs745[ceh-36p3::npp-9::mCherry::BLRP::3xFLAG::npp-9 3'UTR] | C. elegans | AWC neurons are marked with mCherry. AWC nuclei can be isolated by INTACT. |
OH16065 | otIs355 IV; otIs747. | C. elegans | otIs355 [rab-3p::2xNLS::TagRFP] IV. otIs747 [srg-13p::GFP]. PHA neurons are labeled with GFP and RFP. Can be used to isolate PHA by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH16077 | pha-1(e2123) III; otEx7419. | C. elegans | otEx7419 [mgl-1p::GFP + pha-1(+)]. GFP expression labels 4 of the 6 RMD neurons (RMDD and RMDV pairs, but not RMDL/R pair). Maintain at 25C to retain array. |
OH16093 | pha-1(e2123) III; otls356 V; otEx6853. | C. elegans | otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx6853 [srv-3p::GFP + pha-1(+)]. Maintain at 25C to retain array. ADL neurons are marked with GFP and RFP. |
OH16144 | nIs175 IV. | C. elegans | nIs175 [ceh-28p::4xNLS::GFP + lin-15(+)] IV. GFP expression in M4 neurons can be used to isolate M4 by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH16150 | nIs107 III; zfIs10 IV. | C. elegans | nIs107 [tbh-1::GFP] III. zfIs10 [tdc-1::mCherry] IV. RIC neurons are marked with both GFP and mCherry. |
OH16151 | pha-1(e2123) III; ynIs34 IV; otEx7424. | C. elegans | ynIs34 [flp-19p::GFP] IV. otEx7424 [lgc-35p::tagRFP + pha-1(+)]. AIN neurons are marked with GFP and RFP. Maintain at 25C to retain array. |
OH16483 | unc-119(ed3) III; otIs768. | C. elegans | otIs768 [hlh-34p::GFP + unc-119(+)]. GFP expression in AVH neurons. Derived by integration of leEx1692. Can be used to isolate AVH by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH16711 | pha-1(e2123) III; otEx7647. | C. elegans | otEx7647 [ttll-9p(500 bp)::GFP + pha-1(+)]. Maintain at 25C to retain array. OLQ neurons are marked with GFP. Can be used to isolate OLQ by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH16719 | otIs138 X; otIs521. | C. elegans | otIs138 [ser-2(prom3) +rol-6(su1006)] X. otIs521 [eat-4(prom8)::tagRFP + ttx-3::GFP]. OLL neurons are labeled with GFP and RFP. Can be used to isolate OLL by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH16749 | ynIs54 IV; hdIs32. | C. elegans | ynIs54 [flp-20p::GFP] IV. hdIs32 [glr-1::DsRed2]. PVC neurons are labeled with GFP and DsRed2. Can be used to isolate PVC by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH16781 | otIs796 X. | C. elegans | otIs796 [flp-12(promDEL3)::GFP + unc-122p::RFP] X. SMB is marked only with GFP, not red. Can be used to isolate SMB by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). This strain carries unc-122p::RFP as a co-injection marker labeling coelomocytes, which can bleed through the green channel; it is not known which red variant was used in the co-injection marker. Make sure the sorted green cells are also not red. Derived by integration of array originally generated in Kyuhyung Kim's lab. |
OH16917 | pha-1(e2123) III; otEx7693. | C. elegans | otEx7693 [F23H12.7p(RIM)::tagRFP + pha-1(+)]. Maintain at 25C to retain array. RIM neurons marked with RFP. Can be used to isolate RIM by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH16949 | otIs736; evIs111. | C. elegans | otIs736 [cat-4p::mCherry + rol-6(su1006)]. evIs111 [F25B3.3::GFP + dpy-20(+)]. Rollers. Pan-neural GFP expression. The two HSN neurons are marked with both GFP and mCherry. Can be used to isolate HSN by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH16952 | pha-1(e2123) III; otEx7568. | C. elegans | otEx7568 [nlp-17::GFP + pha-1(+)]. Maintain at 25C to retain array. PVQ neurons marked with GFP. Can be used to isolate PVQ by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH17124 | otIs837. | C. elegans | otIs837 [unc-25(prom3)(del1)::GFP]. RME neurons are labeled with GFP. Can be used to isolate RME by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH17211 | pha-1(e2123) III; otEx7567. | C. elegans | otEx7567[nlp-56p::GFP + pha-1(+)]. RMG neurons are labeled with GFP. Can be used to isolate RMG by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH17489 | egIs1; otIs860. | C. elegans | egIs1 [dat-1p::GFP]. otIs860 [flp-33p::tagRFP]. CEP neurons are marked with bright green expression (dat-1p::GFP), and can be sorted by selecting the brightest GFP. there are other cells in which the GFP marker shows up, but expression is faint. Other fluorescing neurons (ADE and PDE) will be double positive (GFP and tagRFP). Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH18011 | pha-1(e2123) III; otEx7947. | C. elegans | otEx7947 [W02A2.5p::GFP + pha-1(+)]. I3 neurons are labeled with GFP. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH18012 | pha-1(e2123) III; otEx7948. | C. elegans | otEx7948 [lgc-8p::mNeonGreen::PH::p10 + pha-1(+)]. I1 neurons are labeled with mNeonGreen. Can be used to isolate I1 neurons by FACS (green-only). Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH18258 | pha-1(e2123) III; otIs355; otEx7950. | C. elegans | otIs355 [rab-3p(prom1)::2xNLS::TagRFP] IV. otEx7950 [clec-166::GFP + pha-1(+)]. Maintain at 25C to select for animals carrying the array. Pan-neuronal nuclear RFP expression. Co-expression of GFP and TagRFP in M5 neuron can be used to isolate M5 by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH18353 | pha-1(e2123) III; otEx8029. | C. elegans | otEx8029 [ceh-19(prom 2)::GFP + pha-1(+)]. Maintain 25C to select for array. Modified ceh-19 promoter fragment drives bright expression of GFP in MC L/R. There is also expression in an additional unidentified cell in the tail but this expression is much much dimmer and typically not visible under the dissecting scope. GFP expression in MC can be used to isolate MC by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
OH18424 | pha-1(e2123) III; otEx8047. | C. elegans | otEx8047 [pks-1p::TagRFP + pha-1(+)]. Maintain at 25C to select for array. TagRFP expression marks CAN neurons. |
PY1157 | oyls17. | C. elegans | oyls17 [gcy-8p::GFP + lin-15(+)]. AFD neurons are marked with GFP. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
SD1241 | gaIs153. | C. elegans | gaIs153 [(pPRSK29) F25B3.3p::FLAG::pab-1 + sur-5::GFP]. Pan-neural expression of FLAG-tagged PAB-1. Detect PAB-1 using anti-FLAG M2 antibody from Sigma. No map data for integrated transgene. |
TU6276 | uIs115; kuIs70. | C. elegans | uIs115 [mec-17p::RFP]. kuIs70 [alr-1p::GFP + rol-6(su1006)]. Rollers. PVM neurons are marked with RFP, allowing FACS sorting by subtraction: FACS sort red cells only to exclude exclude other neurons that are marked with either GFP or both RFP & GFP. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
XE2031 | wpIs107 I. | C. elegans | wpIs107 [F49C12.10p::GFP] I. GFP expression in CAN neurons can be used to isolate CAN by FACS. F49C12.10 also known as nemt-1. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
XE2660 | wpIs40 V; ynIs50. | C. elegans | wpIs40 [unc-47p::mCherry] V. ynIs50 [flp-22p::GFP]. AVL neurons are labeled with GFP and mCherry. Can be used to isolate AVL by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
XE2789 | pha-1(e2123) III; ccIs4595 IV; wpEx482. | C. elegans | ccIs4595 [ceh-24::GFP + rol-6(su1006)]. wpEx482 [ceh-17::NLS::TagRFP + pha-1(+)]. Maintain at 25C to retain array. GFP expression in vulval muscles, m8, and set of neurons in the head. The four SIA neurons are marked with both GFP and RFP. Can be used to isolate SIA by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
XE2835 | wpIs39 X; otIs92. | C. elegans | wpIs39 [unc-47p:mCherry] X. otIs92 [flp-10::GFP]. DVB neurons are marked with GFP and mCherry. Can be used to isolate DVB by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
XE3004 | pha-1(e2123) III; wpEx505. | C. elegans | wpEx505 [ocr-3p::mEGFP + pha-1(+)]. Maintain at 23-25C to select for array. PVP neurons are marked with mEGFP. Can be used to isolate PVP by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). The ocr-3p::mEGFP plasmid used to generate this strain was provided by Dr. Patrick Laurent. |
XE3088 | pha-1(e2123) III; wpEx517. | C. elegans | wpEx517 [srab-20p::Neptune2.5 + pha-1(+)]. Maintain at 25C to select for animals carrying the array. Neptune2.5 expression in PHB neuron can be used to isolate PHB by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
XE3106 | pha-1(e2123) III; otIs707; wpEx525. | C. elegans | otIs707 [bnc-1p(1.8kb)::GFP]. wpEx525 [nlp-38p::NLS::TagRFP + pha-1(+)]. Maintain at 25C to select for animals carrying the array. GFP expression in VA neurons can be used to isolate VA by FACS (exclude TagRFP). Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). |
Alleles contributed by this laboratory
Allele | Type | DNA Change | Protein Change |
---|---|---|---|
wd44 | Allele | substitution | |
wd17 | Allele | ||
ed1 | Allele | ||
wd22 | Allele | ||
wd20 | Allele | ||
wd21 | Allele |