Laboratory Information

NameNC View on WormBase
Allele designationwd
HeadDavid Milton Miller
InstitutionVanderbilt University, Nashville, TN
Address Cell and Developmental Biology
3114 MRB3 BioSciences Building
465 21st Avenue South
Nashville 37240
United States
Website http://exploration.vanderbilt.edu/news/news_worm.htm
Gene classes blr  nab 

Strains contributed by this laboratory

Strain Genotype Species Description
CB4330 unc-4(e2309) II. C. elegans Reference:
LX837 vsIs45. C. elegans vsIs45 [tph-1::GFP]. tph-1::GFP in vsIs45 is expressed exclusively in the NSM neurons in larvae and NSM + HSN in adults, whereas other tph-1::GFP reporters are also expressed in other serotonergic neurons; can be used to image NSM development and for FACS isolation of NSM from L1s. Reference: Nelson JC, Colon-Ramos DA. J Neurosci. 2013 Jan 23;33(4):1366-76.
NC1013 unc-119(ed3) III; wdEx455. C. elegans wdEx455 [acr-14::GFP + unc-119(+)]. Pick non-Unc to maintain.
NC1015 unc-119(ed3) III; wdEx457. C. elegans wdEx457 [F39B2.8::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in pharyngeal muscles, posterior ventral cord motor neurons, tail neurons, and a few head neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.
NC106 unc-37(e262 wd26) I. C. elegans Incompletely-penetrant exploding vulva. wd26 (E580K) is an intragenic revertant of the e262 allele for the backward movement phenotype; it is not known if it also modifies other e262 phenotypes. The wd26 lesion (E580K) is molecularly identical to wd14, wd16, wd17, wd18, etc. Reference: Pflugrad A, et al. Development. 1997 May;124(9):1699-709.
NC138 dpy-20(e1282) IV; wdIs3 X. C. elegans wdIs3[del-1::GFP + dpy-20(+)]. del-1 is expressed in the VB motor neurons beginning the the L2 larval stage. By the end of L2, del-1::GFP is also visible in a few VA motor neurons at the anterior end of the nerve cord. Expression of del-1::GFP in the VAs progresses in a wave from anterior to posterior, with all VAs expressing del-1::GFP by the adult stage. Thus, del-1::GFP is not expressed in the VAs during the L2 period in which unc-4 functions in those cells to establish synaptic inputs but is expressed in the VAs after they have been wired into the ventral cord circuit. del-1::GFP is also expressed in five neurons (VB1, VB2, SABVR, SABVL, VA1) in the retrovesicular ganglion at the anterior end of the ventral nerve cord. During the mid-L2 larval stage, del-1::GFP expression in the ventral nerve cord is largely restricted to the VB class of motor neurons.
NC1469 unc-119(ed3) III; wdEx575. C. elegans wdEx575 [ZC155.2::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expressed in cholinergic motor neurons, head & tail , neurons, and excretory cell. Construct made by Marc Vidal's group at Harvard as part of the promoterome project.
NC1528 unc-119(ed3) III; wdEx595. C. elegans wdEx595 [F08G12.1::GFP + unc-119(+)]. Pick non-Unc to maintain. Construct made by Marc Vidal's group at Harvard as part of the promoterome project.
NC1624 unc-119(ed3) wdIs62 III. C. elegans wdIs62 contains [ceh-12::GFP + unc-119(+)]. Superficially wild-type.
NC168 unc-4(e26) II; dpy-20 IV; wdEx60. C. elegans wdEx60 contains [acr-5::GFP + dpy-20(+)]. Maintain by picking non-Dpy.
NC1686 wdIs51. C. elegans wdIs51 [F49H12.4::GFP + unc-119(+)]; likely integrated in X. GFP expression in PVD.
NC1687 wdIs52. C. elegans wdIs52 [F49H12.4::GFP + unc-119(+)].
NC1700 unc-119(ed3) III; wdEx637. C. elegans wdEx637 [dat-1::3xFLAG::pab-1 + unc-119(+)]. Pick non-Unc to maintain. Reference: Spencer WC, et al. Genome Res. 2011 Feb;21(2):325-41.
NC1730 unc-5(e152) IV; wdIs52. C. elegans wdIs52 [F49H12.4::GFP + unc-119(+)]. PVD defects in primary branch guidance, number of secondary branches, and tertiary branches are longer than wild-type.
NC1750 hdIs32 III; gvEx173. C. elegans hdIs32 [glr-1::DsRed2]. gvEx173 [opt-3::GFP + rol-6(su1006)]. Maintain by picking Rollers, which should be GFP+. hdIs32 might be prone to silencing.
NC190 wdIs6 II; dpy-20(e1282) IV. C. elegans wdIs6 [del-1::GFP + dpy-20(+)]. Embryonic GFP expression in SABVR, SABVL, VBs in mid-L2, VAs in mid-L3.
NC197 wdIs4 II; dpy-20(e1282) IV. C. elegans wdIs4 [unc-4::GFP + dpy-20(+)] II. Slightly Unc. GFP expression mosaic, occasional DA axon guidance defects. Embryonic expression: I5, DA, SABS; L1: AVF, VA; Late L3: VC. Early L1: GFP bright in I5, SABs, DA8/9, dim in DA1.
NC2020 coq-2(ok1066) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); juIs14 IV; wdEx658. C. elegans juIs14 [(pSC205) acr-2p::GFP + lin-15(+)] IV. wdEx658 [unc-25p::mCherry::unc-54 3'UTR + unc-119(+)]. Pick mCherry+ to maintain wdEx658. Homozygous larval lethal coq-2 mutation balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ced-9 homozygotes (maternal effect lethal). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT with GFP+ pharynx and mCherry+, and check for correct segregation of progeny to maintain.
NC216 lin-15B&lin-15A(n765); wdEx75. C. elegans wdEx75 [acr-5::GFP + lin-15(+)]. Expressed in B-type motor neurons.
NC2484 wdIs5 III; dpy-20(e1282) IV. C. elegans wdIs5 [unc-4::GFP + dpy-20(+)] III. Reference: Lickteig KM, et al. J Neurosci. 2001 Mar 15;21(6):2001-14.
NC2537 unc-119(ed3) III; wdEx848. C. elegans wdEx848 [mec-4::mCherry + unc-119(+)]. Pick non-Unc to maintain. mCherry expression in all six touch neurons. Array generated by bombardment; ~90% transmission rate. Reference: Smith CJ, O'Brien T, et al. Neuron. 2013 July 24; 79.
NC268 unc-4(wd44) II. C. elegans Unc. Reference: Winnier AR, et al. Genes Dev. 1999 Nov 1;13(21):2774-86.
NC279 del-1(ok150) X. C. elegans 2 kb deletion mutant made by OMRF Knockout Group. Presumptive null. No obvious phenotype. Primers used: EL1: GAAACGGTGAGTGCCAATTT. ER1: AGTGCTGTCACACCAAGCAC. IL1: AAACCAACTGACCCAAGGTG. IR1: TATCTAGGGTCCGCACAACC. Left breakpoint sequence: AGGTTGACAAATTGTTGCGA. Right breakpoint sequence: CGCTTATTAAAAAATAATAT.
NC292 acr-5(ok182) III. C. elegans No obvious phenotype. 1.5 kb deletion of acr-5 produced by Moulder/Barstead at OMRF. Left breakpoint sequence: TGGGTGATGCTATATGCACA. Right breakpoint sequence: TAGACTTCCGAGCAATAATTC.
NC293 acr-5(ok180) III. C. elegans No obvious phenotype. 2 kb deletion of acr-5 produced by Moulder/Barstead at OMRF. Deletion removes all 4 transmembrane domains. This is likely a null allele. Left breakpoint sequence (includes repeated sequence): TTTTTAATTATCCGTAATTTTTTAATTATCCGTAAT. Right breakpoint sequence: AACATCTTTAATCGATTTAT.
NC300 dpy-20(e1282) IV; wdIs5. C. elegans wdIs5 [unc-4p::~1.5 exons of the unc-4 gene::GFP + (pMH86) dpy-20(+)]. Slightly Unc. GFP expression mosaic, occasional DA axon guidance defects. Embryonic expression: I5, DA, SABs; L1: AVF, VA; late L3: VC.
NC3292 oig-1(wd114[oig-1::gfp11x7]) III. C. elegans Superficially wild-type. wd114 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous oig-1 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395.
NC3381 lev-10(wd116[lev-10::gfp11x7]) I. C. elegans wd116 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous lev-10 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395.
NC3390 oig-1(wd114[oig-1::gfp11x7]) III; wdEx1034. C. elegans wdEx1034 [rab-3p::GFP1-10 + myo-2p::mCherry]. Pick mCherry+ to maintain. wd114 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous oig-1 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395.
NC3492 lev-10(wd116[lev-10::gfp11x7]) I; wdEx1086. C. elegans wdEx1086 [myo-3p::GFP1-10 + myo-2p::mCherry]. Pick mCherry+ to maintain. wd116 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous lev-10 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395.
NC3493 lev-10(wd116[lev-10::gfp11x7]) I; wdEx1087. C. elegans wdEx1087 [ttr-39p::GFP1-10 + myo-2p::mCherry]. Pick mCherry+ to maintain. wd116 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous lev-10 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395.
NC3540 otIs458. C. elegans otIs458[ceh-63p::GFP + unc-119(+)]. GFP expression in DVC neurons. Can be used to isolate DVC by FACS. Derived by outcrossing parental strain OH11974 to remove him-5(e1490). Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
NC3545 lev-10(wd116[lev-10::gfp11x7]) I; wdEx1103. C. elegans wdEx1103 [acr-2p::GFP1-10 + myo-2p::mCherry]. Pick mCherry+ to maintain. wd116 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous lev-10 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395.
NC37 unc-37(wd17) I; unc-4(e2322) II. C. elegans unc-37(wd17) is a dominat suppressor of unc-4(e2322ts). unc-37(wd17) has no phenotype alone.
NC3738 wdIs132; uIs152. C. elegans wdIs132 [gcy-35::GFP]. uIs152 [mec-3::RFP]. AVM neurons are labeled with GFP and RFP. Can be used to isolate AVM by FACS. wdIs132 arose from spontaneous integration of kyEx1162 [gcy-35::GFP]. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
NC3792 ynIs37 III; ufIs26. C. elegans ynIs37 [flp-13p::GFP] III. ufIs26 [unc-4p::mCherry + lin-15(+)]. I5 neurons are labeled with GFP and RFP. Can be used to isolate I5 by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
NC467 acr-5(ok205) III. C. elegans No obvious phenotype. 2.4 kb deletion of acr-5 produced by Moulder/Barstead at OMRF.
NC571 dpy-20 (e1282) IV; wdIs20. C. elegans wdIs20 [unc-4p::snb-1::GFP + dpy-20(+)]. Animals are Lon, likely due to dpy-20(+) overexpression. Punctate GFP expression observed in dorsal nerve cord, ventral nerve cord, VC4, and VC5. Reference: Lickteig KM, et al. J Neurosci. 2001 Mar 15;21(6):2001-14.
NC574 wdIs22. C. elegans wdIs22 [acr-5p::YFP + rol-6(su1006)]. Integrated array derived from wdEx223. Maintain by picking Rollers.
NC694 unc-119(ed1) III; wdEx257. C. elegans wdEx257 [unc-4::3XFLAG::pab-1 + (pSV17) unc-119 minigene]. Animals exhibit slight forward movement defect. Good antibody staining witih anit-FLAG M2 antibody (Sigma). 100% penetrant array. Expressed in all unc-4 neurons. Ectopically expressed in at least one head neuron (dorsal to pharynx, between two bulbs).
NC698 wdEx258. C. elegans wdEx258 [twk-30::GFP + myo-3::DsRed2)]. GFP expression in perinuclear motor neurons.
NC782 unc-119(ed3) III; wdEx290. C. elegans wdEx290 [acr-15::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.
NC821 unc-119(ed3) III; wdEx329. C. elegans wdEx329 [F09C3.2::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in ventral nerve cord. Construct made by M. Vidal lab; candidate unc-37 target gene.
NC844 unc-119(ed3) III; wdEx345. C. elegans wdEx345 [F55C12.4::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.
NC845 unc-119(ed3) III; wdEx346. C. elegans wdEx346 [tig-1::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in head neurons, tail neurons. head muscles, and vulva. Construct made by M. Vidal lab; candidate unc-37 target gene.
NC847 unc-119(ed3) III; wdEx348. C. elegans wdEx348 [C13G3.1::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.
NC85 unc-37(wd17wd22)/dpy-14(e188) I. C. elegans Heterozygotes are WT and segregate WT, Dpys and Steriles.
NC850 unc-119(ed3) III; wdEx351. C. elegans wdEx351 [tsp-7::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in motor neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.
NC852 unc-119(ed3) III; wdEx353. C. elegans wdEx353 [Y34D9B.1a::GFP + unc-119(+)]. mig-1::GFP construct made by Marc Vidal's group at Harvard as part of the promoterome project; unc-37 target gene. GFP expression observed in all classes of VNC motor neurons, head & tail neurons, body wall muscle, and intestine.
NC865 unc-119(ed3) III; wdEx359. C. elegans wdEx359 [F29G6.2::GFP + unc-119(+)]. GFP+ seen in ventral nerve cord. Pick non-Unc worms that are GFP+.
NC902 unc-119(ed3) III; wdEx381. C. elegans wdEx381 [F55C12.4::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.
NC92 unc-37(wd17wd20)/dpy-14(e188) I. C. elegans Heterozygotes are WT and segregate WT, Dpys, and Steriles.
NC93 unc-37(wd17wd21)/dpy-14(e188) I. C. elegans Heterozygotes are WT and segregate WT, Dpys and Steriles.
NC972 wdEx419. C. elegans wdEx419 [acr-16::GP + rol-6(su1006)]. Rollers. Maintain by picking rollers. GFP expression detectable in body wall muscle and DB motor neurons.
NC987 wdEx445. C. elegans wdEx445 [T27E9.9::GFP + rol-6(su1006))]. Pick rollers to maintain. GFP expression in DA and DB neurons during L1 stage, low levels of expression in VA neurons in later stages.
NC996 wdEx451. C. elegans wdEx451 [Y71D11A.5::GFP + rol-6(su1006))]. Pick rollers to maintain. GFP expression in VD, DD, and AS neurons, multiple head and tail neurons, and muscles.
OH12503 otIs520 X. C. elegans otIs520 [eat-4(prom11)::GFP + ttx-3::mCherry] X. AIM is marked only with GFP, not mCherry. Can be used to isolate AIM by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH15507 otIs439; hdIs32. C. elegans otIs439 [lad-2p::GFP + pha-1(+)]. hdIs32 [glr-1::DsRed2]. SMD neurons are labeled with GFP and DsRed2. Can be used to isolate SMD by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH16052 otIs745[ceh-36p3::npp-9::mCherry::BLRP::3xFLAG::npp-9 3'UTR] C. elegans AWC neurons are marked with mCherry. AWC nuclei can be isolated by INTACT.
OH16065 otIs355 IV; otIs747. C. elegans otIs355 [rab-3p::2xNLS::TagRFP] IV. otIs747 [srg-13p::GFP]. PHA neurons are labeled with GFP and RFP. Can be used to isolate PHA by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH16077 pha-1(e2123) III; otEx7419. C. elegans otEx7419 [mgl-1p::GFP + pha-1(+)]. GFP expression labels 4 of the 6 RMD neurons (RMDD and RMDV pairs, but not RMDL/R pair). Maintain at 25C to retain array.
OH16093 pha-1(e2123) III; otls356 V; otEx6853. C. elegans otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx6853 [srv-3p::GFP + pha-1(+)]. Maintain at 25C to retain array. ADL neurons are marked with GFP and RFP.
OH16150 nIs107 III; zfIs10 IV. C. elegans nIs107 [tbh-1::GFP] III. zfIs10 [tdc-1::mCherry] IV. RIC neurons are marked with both GFP and mCherry.
OH16151 pha-1(e2123) III; ynIs34 IV; otEx7424. C. elegans ynIs34 [flp-19p::GFP] IV. otEx7424 [lgc-35p::tagRFP + pha-1(+)]. AIN neurons are marked with GFP and RFP. Maintain at 25C to retain array.
OH16483 unc-119(ed3) III; otIs768. C. elegans otIs768 [hlh-34p::GFP + unc-119(+)]. GFP expression in AVH neurons. Derived by integration of leEx1692. Can be used to isolate AVH by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH16711 pha-1(e2123) III; otEx7647. C. elegans otEx7647 [ttll-9p(500 bp)::GFP + pha-1(+)]. Maintain at 25C to retain array. OLQ neurons are marked with GFP. Can be used to isolate OLQ by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH16719 otIs138 X; otIs521. C. elegans otIs138 [ser-2(prom3) +rol-6(su1006)] X. otIs521 [eat-4(prom8)::tagRFP + ttx-3::GFP]. OLL neurons are labeled with GFP and RFP. Can be used to isolate OLL by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH16749 ynIs54 IV; hdIs32. C. elegans ynIs54 [flp-20p::GFP] IV. hdIs32 [glr-1::DsRed2]. PVC neurons are labeled with GFP and DsRed2. Can be used to isolate PVC by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH16781 otIs796 X. C. elegans otIs796 [flp-12(promDEL3)::GFP + unc-122p::RFP] X. SMB is marked only with GFP, not red. Can be used to isolate SMB by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). This strain carries unc-122p::RFP as a co-injection marker labeling coelomocytes, which can bleed through the green channel; it is not known which red variant was used in the co-injection marker. Make sure the sorted green cells are also not red. Derived by integration of array originally generated in Kyuhyung Kim's lab.
OH16917 pha-1(e2123) III; otEx7693. C. elegans otEx7693 [F23H12.7p(RIM)::tagRFP + pha-1(+)]. Maintain at 25C to retain array. RIM neurons marked with RFP. Can be used to isolate RIM by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH16949 otIs736; evIs111. C. elegans otIs736 [cat-4p::mCherry + rol-6(su1006)]. evIs111 [F25B3.3::GFP + dpy-20(+)]. Rollers. Pan-neural GFP expression. The two HSN neurons are marked with both GFP and mCherry. Can be used to isolate HSN by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH16952 pha-1(e2123) III; otEx7568. C. elegans otEx7568 [nlp-17::GFP + pha-1(+)]. Maintain at 25C to retain array. PVQ neurons marked with GFP. Can be used to isolate PVQ by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH17124 otIs837. C. elegans otIs837 [unc-25(prom3)(del1)::GFP]. RME neurons are labeled with GFP. Can be used to isolate RME by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
OH17211 pha-1(e2123) III; otEx7567. C. elegans otEx7567[nlp-56p::GFP + pha-1(+)]. RMG neurons are labeled with GFP. Can be used to isolate RMG by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
SD1241 gaIs153. C. elegans gaIs153 [(pPRSK29) F25B3.3p::FLAG::pab-1 + sur-5::GFP]. Pan-neural expression of FLAG-tagged PAB-1. Detect PAB-1 using anti-FLAG M2 antibody from Sigma. No map data for integrated transgene.
temp_name304 coq-2(ok1066) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); juIs14 IV; wdEx658 [unc-25p::mCherry::unc-54 3'UTR + unc No mCherry+ animals
TU6276 uIs115; kuIs70. C. elegans uIs115 [mec-17p::RFP]. kuIs70 [alr-1p::GFP + rol-6(su1006)]. Rollers. PVM neurons are marked with RFP, allowing FACS sorting by subtraction: FACS sort red cells only to exclude exclude other neurons that are marked with either GFP or both RFP & GFP. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
XE2660 wpIs40 V; ynIs50. C. elegans wpIs40 [unc-47p::mCherry] V. ynIs50 [flp-22p::GFP]. AVL neurons are labeled with GFP and mCherry. Can be used to isolate AVL by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
XE2789 pha-1(e2123) III; ccIs4595 IV; wpEx482. C. elegans ccIs4595 [ceh-24::GFP + rol-6(su1006)]. wpEx482 [ceh-17::NLS::TagRFP + pha-1(+)]. Maintain at 25C to retain array. GFP expression in vulval muscles, m8, and set of neurons in the head. The four SIA neurons are marked with both GFP and RFP. Can be used to isolate SIA by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
wd44 Allele substitution
wd17 Allele
ed1 Allele
wd22 Allele
wd20 Allele
wd21 Allele