Laboratory Information

NameMU View on WormBase
Allele designationbw
HeadBruce C Wightman
InstitutionMuhlenberg College
Address Biology Department
2400 Chew St
Allentown 18104-5564
United States
Website http://www.muhlenberg.edu/depts/biology/faculty/wightman/page.htm
Gene classes fax  prmn 

Strains contributed by this laboratory

Strain Genotype Species Description
LA275 nhr-67(pf2) IV. C. elegans Pvl. Egl. Weaker allele than pf88 and pf159.
LA691 nhr-67(pf159) IV. C. elegans Pvl. Egl. Poor mating.
MU1080 unc-2(e55) fax-1(gm83) X. C. elegans unc-2(e55) is very inactive. gm83 is a Unc (forward kinker). Double shows both phenotypes.
MU1085 bwIs2. C. elegans bwIs2 [flp-1::GFP + rol-6(su1006)]. Segregates >90% Rollers and 100% GFP+. Expresses GFP in the AVK neurons. Insertion site not mapped.
MU1147 bwIs4. C. elegans bwIs4 [fax-1::GFP + rol-6(su1006)]. Rollers. GFP reporter with expression in various neurons and DTC.
MU1255 nhr-67(tm2217) IV/nT1 [qIs51] (IV;V). C. elegans Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP tm2217 homozygotes (arrested L1 larvae). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain.
MU1268 bwIs6. C. elegans bwIs6 [nhr-67::GFP + rol-6(su1006)]. Rollers.
MU1269 nhr-67(pf88) IV/nT1 [qIs51] (IV;V). C. elegans Heterozygotes are WT and GFP+. Pick GFP+ to maintain -- viable pf88 homozygotes (Pvl Egl) can overtake the bablanced population. nT1[qIs51] is homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Reference: Verghese E, et al. Dev Biol. 2011 Aug 15;356(2):516-28.
MU1289 nhr-67(pf88) IV. C. elegans Pvl. Egl. Poor mating.
NG2267 fax-1(gm27) lon-2(e678) X. C. elegans Unc and Lon. ME2-3.
This laboratory hasn't submitted any alleles to the CGC.