Laboratory Information
| Name | MH View on WormBase |
|---|---|
| Allele designation | ku |
| Head | Min Han |
| Institution | University of Colorado at Boulder |
| Address | University of Colorado UCB 347, MCDB Boulder 80309 United States |
| Website | https://www.colorado.edu/lab/han |
| Gene classes | acs ain dli ect glv ksr mek mnp nprl rho rrp slr sptl sur disl vglm vgln dbt ate cerk dus fath |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| MH1019 | soc-2(ku167) IV. | C. elegans | No obvious phenotype alone. ku167 suppresses let-60(n1046). Previously called sur-8. |
| MH1113 | dpy-20(e1282) IV; sur-5(ku74) X. | C. elegans | Dpy. |
| MH1131 | soc-2(ku167) let-60(n1046) IV. | C. elegans | Less than 5% Muv. Previously called sur-8(ku167). |
| MH1152 | egl-26(ku228) II. | C. elegans | 83% Egl. 4% Sterile. |
| MH1157 | him-5(e1490) V; egl-13(ku194) X. | C. elegans | ku194 is a loss of function allele, likely to be molecular null. Connection of gonad defective, >95% Egl. Anchor cell and uterine seam cell do not fuse. Males can mate. Hermaphrodites are very difficult to mate. Previously called cog-2(ku194). |
| MH1292 | sur-6(ku123) I. | C. elegans | Weak Vul phenotype. Suppressor of gain-of-function Ras. ku123 causes a C302Y substitution. |
| MH1301 | unc-83(ku18) V. | C. elegans | Point mutation W257-> stop codon. Disrupts P cell nuclear migration at 25C. This leads to an Egl, Unc worm. P cell nuclear migration is normal at 15C. hyp7 nuclear migration is normal at all temperatures. |
| MH1317 | kuIs29 V. | C. elegans | kuIs29 [egl-13p::GFP + unc-119(+)] V. egl-13 is the new gene name for cog-2. Transcriptional fusion of GFP to egl-13 gene. Nuclear localized. Bright expression in body wall muscles, expressed in uterine pi lineage, extensive neuronal expression. Note that a very low penetrance Cog phenotype is seen in this strain. Transgenes with egl-13 promoter can cause Cog phenotype. Conflicting map data: Wendy Hanna-Rose mapped kuIs129 to the left of dpy-11; Shi lab reported it close to gon-10 and unc-76. |
| MH1346 | kuIs35. | C. elegans | kuIs35 [sem-4::GFP]. |
| MH1564 | kuIs36 II. | C. elegans | kuIs36 [egl-26::GFP + unc-119(+)] II. Transcriptional fusion of GFP to egl-26 gene. Nuclear localized. Embryonic expression. Expressed in somatic gonad (uterus and spermatheca) and vulva in vulE and vulB in L4. Spermatheca expression persists into adulthood. Expressed in pharyngeal-intestinal junction cells and in tail. |
| MH17 | sur-2(ku9) I. | C. elegans | 100% bag of worms. Low percentage of dead larvae. Slightly dumpy. ku9 completely suppresses let-60(n1046) Muv phenotype. |
| MH1914 | lin-40(ku285) V. | C. elegans | Animals grow slowly and are semi-sterile. Vulval lineage defective. |
| MH1946 | dli-1(ku266)/+ IV; him-5(e1490) V. | C. elegans | Heterozygotes are WT and segregate WT and Sterile animals with a protruding vulva. Throws males. Clone several WT to recover heterozygote. Cytoplasmic dynein light intermediate chain. 9/02: ku266 is not an L to stop; it is a W117 to a stop (TGG to TGA). Sandhya Koushika. |
| MH1955 | nhr-25(ku217) X. | C. elegans | Temperature sensitive Egl. |
| MH2051 | kuIs55. | C. elegans | kuIs55 [lon-3::GFP + unc-119(+)]. Rollers. The kuIs55 lon-3::GFP transgene does not rescue the Lon phenotype of lon-3 mutants, but instead causes an adult Rol (Roller) phenotype both in lon-3 mutants and in wild-type backgrounds. Reference: Suzuki Y, et al. 2002. Genetics 162: 1631–1639. |
| MH2211 | unc-29(e1072); sur-6(ku123); kuIs57. | C. elegans | kuIs57 [col-10p::lin-45(gf) + sur-5::GFP]. Reference: Yoder JH, et al. EMBO J. 2004 Jan 14;23(1):111-9. |
| MH2230 | dph-3(ku432) . | C. elegans | Superficially wild type. Maintain under standard conditions. Reference: Kims S, et al. Genetics. 2010 Aug;185(4):1235-47. |
| MH2285 | lin-66(ku423) IV/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygotes are Unc. ku423 homozygotes have delayed heterochronic phenotype and are L4 lethal. |
| MH2294 | scc-3(ku263)/lin-25(n545) V. | C. elegans | Heterozygotes are WT and segregate WT, lin-25 homozygotes (which are temperature sensitive: at 25C adult hermaphrodites are vulvaless, at 15C 8% of adult hermaphrodites are vulvaless), and scc-3 homozygotes which are Pvl and Sterile (vulval morphogenesis defects and defects in sister-chromatin cohesion). |
| MH231 | let-60(n1046) IV; ksr-1(ku68) X. | C. elegans | Semi-dominant suppressor of let-60(n1046). Strong reduction of function mutation. At 20C: <1% Muv, 24% Egl, 6% larval lethal, and SM migration defects. |
| MH2354 | swsn-1(ku355) V. | C. elegans | Synthetic lethal with lin-35(n745). Temperature sensitive. |
| MH2385 | ain-1(ku322) X. | C. elegans | WT looking worms. 40% alae gap. ku322 suppresses lin-31(lf) Muv phenotype. Received new stock 8/2006 from Han lab. |
| MH2407 | ect-2(ku427) II. | C. elegans | Bag. Missing Pn.p cell. |
| MH2430 | cbp-1(ku258) III. | C. elegans | Semidominant suppressor of let-60(n1046). |
| MH2805 | kuIs70. | C. elegans | kuIs70 [alr-1p::GFP + rol-6(su1006)]. Rollers. alr-1p::GFP expression is visible in embryonic and adult tissues. Reference: Tucker M, et al. Mol Biol Cell. 2005 Oct;16(10):4695-704. |
| MH3084 | ain-2(tm1863) I; ain-1(ku322) X. | C. elegans | Double mutants displayed a severe defect in seam-cell development, implicating a retarded heterochronic phenotype. Protruding vulva phenotype. Increased number of seam cells. Reference: Zhang L, et al. Mol Cell. 2007 Nov 30;28(4):598-613. PMID: 18042455 |
| MH351 | let-60(sy101sy127)/dpy-20(e1282) IV. | C. elegans | Heterozygotes are WT and segregate WT, Dpys and lethals. |
| MH37 | mpk-1(ku1) unc-32(e189) III. | C. elegans | Unc. ku1 pka sur-1(ku1). |
| MH4176 | ain-1(ku425) X. | C. elegans | Superficially wild-type. Identified in a screen for suppressors of the Multivulva (Muv) phenotype in lin-31 loss-of-function (lf) mutants. Reference: Ding L, et al. Mol Cell. 2005 Aug 19;19(4):437-47. PMID: 16109369. |
| MH4177 | ain-1(tm3681) X. | C. elegans | Developmental timing delay. Maintain under normal conditions. Reference: Zhang X, et al. PNAS. 2011 Nov 1;108(44):17997-8002. |
| MH4429 | ain-2(tm2432) I. | C. elegans | Superficially wild-type. Maintain under normal conditions. Reference: Zhang X, et al. PNAS. 2011 Nov 1;108(44):17997-8002. |
| MH4799 | elt-1(ku491) IV. | C. elegans | Reference: Cohen ML, et al. PLoS Genet. 2015 Mar 27;11(3):e1005099. |
| MH4810 | elt-1(ku491) IV; wIs51 V; daf-12(rh61rh411) X; kuEx194. | C. elegans | wIs51 [SCMp::GFP + unc-119(+)] V. kuEx194 [elt-1(+) + sur-5p::DsRed]. GFP expression in seam cells. Pick DsRed+ animals to maintain. In a daf-12(WT) background, elt-1(ku491) exhibits some precocious fusion of seamcells and gaps in alae. elt-1(ku491); daf-12(rh61rh411) double mutants have more sever heterochronic phenotypes including seamcell proliferation and bursting vulvae. Reference: Cohen ML, et al. PLoS Genet. 2015 Mar 27;11(3):e1005099. |
| MH4920 | unc-119(ed3) III; kuIs102. | C. elegans | kuIs102 [vgln-1p::vgln-1::GFP + Cbr-unc-119(+)]. Integrated translational VGLN-1::GFP fusion protein. Reference: Zabinsky RA, et al. G3 (Bethesda). 2017 Jun 2. pii: g3.117.043414. doi: 10.1534/g3.117.043414. PMID: 28576776 |
| MH5015 | kuIs118 II; unc-119(ed3) III. | C. elegans | kuIs118 [daf-15p::daf-15::mCherry + Cbr-unc-119(+)] II. kuIs118 is a single copy insertion into ttTi5605 via CRISPR/Cas9. Superficially wild-type. mCherry expression observed throughout the body. mCherry detected throughout development by western blot with anti-mCherry antibody, with highest expression levels in early larval stages. Reference: Sewell AK, et al. "The TORC1 phosphoproteome in C. elegans reveals roles in transcription and autophagy” iScience, 20 May 2022, 104186. https://www.sciencedirect.com/science/article/pii/S2589004222004564. |
| MH5197 | nprl-3(ku540) IV. | C. elegans | Superficially wildtype. Homozygous nprl-3(ku540) can suppress the early larval arrest phenotype of mmBCFA deficiency mutants elo-5(gk208) and cgt-1(tm1027) cgt-3(tm504). References: Zhu H, et al. Elife. 2013 May 21;2:e00429. Zhu H, Sewell AK, Han M. Genes Dev. 2015 Jun 15;29(12):1218-23. |
| MH5239 | prx-5(ku517) II. | C. elegans | Suppresses the developmental arrest of elo-5(gk208). Slightly delayed development/growth. |
| MH538 | mek-2(ku114) I; let-60(n1046) IV. | C. elegans | |
| MH734 | ksr-1(ku68) X. | C. elegans | At 20C: 7% Egl, 22% larval lethal. Vulval lineages WT. |
| MH801 | sur-7(ku119) X. | C. elegans | No obvious morphological phenotype on its own. Good suppressor of Muv of let-60(n1046). |
| OP50/cytR- | E. coli [cytR-] | Escherichia coli | Bacteria. Kanamycin-resistant E. coli. cytR- mutation in OP50 background causes elavated nucleotide levels similar to HT115. A mutation in CytR- was introduced into the OP50 strain by recombineering. Bacterial cells were transformed with pSIM8 plasmid before using as hosts for recombineering. Targeting substrate DNA fragments were amplified by PCR and subcloned into TOPO cloning vector for sequence verification before being electroporated into the host bacterial cells. The primer set used for cloning targeting cytR substrate DNAs (to replace cytR+ with the cytR- mutation identified in HT115 strain): 5'- GCCAGGCGAGGAGTGAGTGTG-3'/5'-AGCGGCGGGCCTTTGACC-3'. Reference: Chi C, et al. Genes Dev. 2016 Feb 1;30(3):307-20. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| ku167 | Allele | substitution | |
| ku212 | Allele | ||
| ku74 | Allele | ||
| ku228 | Allele | substitution | |
| ku194 | Allele | substitution | nonsense |
| ku123 | Allele | substitution | |
| ku18 | Allele | ||
| ku9 | Allele | substitution | nonsense |
| ku298 | Allele | substitution | |
| ku285 | Allele | substitution | splice_site |
| ku266 | Allele | ||
| ku217 | Allele | substitution | |
| ku156 | Allele | ||
| ku432 | Allele | deletion | |
| ku423 | Allele | substitution | nonsense |
| ku263 | Allele | substitution | nonsense |
| ku68 | Allele | substitution | |
| ku355 | Allele | ||
| ku322 | Allele | substitution | nonsense |
| ku427 | Allele | substitution | |
| ku258 | Allele | substitution | |
| ku1 | Allele | ||
| ku491 | Allele | substitution | |
| ku540 | Allele | substitution | |
| ku114 | Allele | substitution | slient |
| ku51 | Allele | substitution | |
| ku112 | Allele | substitution | |
| ku119 | Allele | substitution | splice_site |
| ku233 | Allele | substitution | |
| ku354 | Allele | substitution |