Laboratory Information

NameMG View on WormBase
Allele designationxs
HeadMichael Glotzer
InstitutionUniversity of Chicago, Chicago, IL
Address 920 E 58th St.
Chicago 60637
United States
Gene classes csc  icp  kle  saps  smc  syn 

Strains contributed by this laboratory

Strain Genotype Species Description
MG152 xsIs3 I. C. elegans N2 with integrated array pJH4.52. xsIs3[hisH2B::GFP; rol-6(su1006)]. 39.9.1 Must be kept at 25C to avoid germline-silencing.
MG155 xsIs4 IV. C. elegans xsIs4 [hisH2B::GFP + rol-6(su1006)]. N2 with integrated complex-array pJH4.52. Must be kept at 25C to avoid germline-silencing.
MG170 zen-4(or153) IV; xsEx6. C. elegans xsEx6 [zen-4::GFP + rol-6(su1006)]. Maintain at 25C. Rollers.
MG278 K08E3.5(ok233) III. C. elegans TI223.E1: AGACTTGAAGGAAACGCGAA. TI223.E2: AATCAAATTGAAACGGCTCG. TI223.I1: AATCCTTGCCAACCAAACAG. TI223.I2: CGTAGCATCCTTGGACCAGT. This strain was provided by the C. elegans Gene Knockout Project at OMRF, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.
MG589 xsSi3 II. C. elegans xsSi3 [GFP::utrophin + Cbr-unc-119(+)].  Superficially wild-type.  Carries a germline-expressed actin probe derived from the calponin homology domain of utrophin.  
MG617 xsSi5 II. C. elegans xsSi5 [pie-1p::GFP::ani-1(AH+PH)::pie-1 3'UTR + Cbr-unc-119(+)] II. The RhoA biosensor consists of GFP fused to the C-terminal portion of C. elegans anillin, which contain its conserved region (AH) and pleckstrin homology (PH) domain. It lacks the N-terminal myosin- and actin-binding domains but retains its RhoA-binding domain. [NOTE: xsSi5 was originally published as mgSi5.] References: Tse YC, et al. Mol Biol Cell. 2012 Oct;23(20):4020-31.
MG685 xsSi43 II; unc-119(ed3) III. C. elegans xsSi43 [cyk-4p::cyk-4::GFP::pie-1 3'UTR + Cbr-unc-119(+)] II. CYK-4::GFP fusion protein expressed in germline and embryo. [NOTE: previously published as mgSi43.] Reference: Zhang D & Glotzer M. Elife. 2015 Aug 7;4.
MG8 csc-1(t1171) dpy-2(e8)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-3(e1147) IV. C. elegans Heterozygotes are WT and segregate WT, DpyUncs, and Dpys which give only dead eggs. Throws males.
MG827 unc-119(ed3) III; xsSi36 IV. C. elegans xsSi36 [myo-2p::GFP(Y66C)::let-858 3'UTR + Cbr-unc-119(+) IV:13,048,924]. This strain contains a non-fluorescent GFP that can be used to enrich for CRISPR mediated, oligodirected homologous recombination. [NOTE: previously published as mgSi36.]  Reference: Zhang D & Glotzer M. 2014. Efficient site-specific editing of the C. elegans genome. doi:
This laboratory hasn't submitted any alleles to the CGC.