Laboratory Information
| Name | MG View on WormBase |
|---|---|
| Allele designation | xs |
| Head | Michael Glotzer |
| Institution | University of Chicago, Chicago, IL |
| Address | 920 E 58th St. Chicago 60637 United States |
| Website | http://mgcb.uchicago.edu/faculty/glotzer/ |
| Gene classes | csc icp kle saps smc syn |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| MG152 | xsIs3 I. | C. elegans | N2 with integrated array pJH4.52. xsIs3[hisH2B::GFP; rol-6(su1006)]. 39.9.1 Must be kept at 25C to avoid germline-silencing. |
| MG155 | xsIs4 IV. | C. elegans | xsIs4 [hisH2B::GFP + rol-6(su1006)]. N2 with integrated complex-array pJH4.52. Must be kept at 25C to avoid germline-silencing. |
| MG170 | zen-4(or153) IV; xsEx6. | C. elegans | xsEx6 [zen-4::GFP + rol-6(su1006)]. Maintain at 25C. Rollers. |
| MG278 | K08E3.5(ok233) III. | C. elegans | TI223.E1: AGACTTGAAGGAAACGCGAA. TI223.E2: AATCAAATTGAAACGGCTCG. TI223.I1: AATCCTTGCCAACCAAACAG. TI223.I2: CGTAGCATCCTTGGACCAGT. This strain was provided by the C. elegans Gene Knockout Project at OMRF, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. http://www.mutantfactory.ouhsc.edu/ |
| MG589 | xsSi3 II. | C. elegans | xsSi3 [GFP::utrophin + Cbr-unc-119(+)]. Superficially wild-type. Carries a germline-expressed actin probe derived from the calponin homology domain of utrophin. |
| MG617 | xsSi5 II. | C. elegans | xsSi5 [pie-1p::GFP::ani-1(AH+PH)::pie-1 3'UTR + Cbr-unc-119(+)] II. The RhoA biosensor consists of GFP fused to the C-terminal portion of C. elegans anillin, which contain its conserved region (AH) and pleckstrin homology (PH) domain. It lacks the N-terminal myosin- and actin-binding domains but retains its RhoA-binding domain. [NOTE: xsSi5 was originally published as mgSi5.] References: Tse YC, et al. Mol Biol Cell. 2012 Oct;23(20):4020-31. |
| MG685 | xsSi43 II; unc-119(ed3) III. | C. elegans | xsSi43 [cyk-4p::cyk-4::GFP::pie-1 3'UTR + Cbr-unc-119(+)] II. CYK-4::GFP fusion protein expressed in germline and embryo. [NOTE: previously published as mgSi43.] Reference: Zhang D & Glotzer M. Elife. 2015 Aug 7;4. |
| MG8 | csc-1(t1171) dpy-2(e8)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-3(e1147) IV. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs, and Dpys which give only dead eggs. Throws males. |
| MG827 | unc-119(ed3) III; xsSi36 IV. | C. elegans | xsSi36 [myo-2p::GFP(Y66C)::let-858 3'UTR + Cbr-unc-119(+) IV:13,048,924]. This strain contains a non-fluorescent GFP that can be used to enrich for CRISPR mediated, oligodirected homologous recombination. [NOTE: previously published as mgSi36.] Reference: Zhang D & Glotzer M. 2014. Efficient site-specific editing of the C. elegans genome. doi: http://dx.doi.org/10.1101/007344. |
This laboratory hasn't submitted any alleles to the CGC.