Laboratory Information
| Name | MAH View on WormBase |
|---|---|
| Allele designation | sq |
| Head | Malene Hansen |
| Institution | Buck Institute for Research on Aging |
| Address | 8001 Redwood Blvd Novato 94945 United States |
| Website | https://www.buckinstitute.org/lab/hansen-lab/ |
| Gene classes | argk |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| GR1971 | mgEx779. | C. elegans | mgEx779 [lipl-4p::K04A8.5p::lipl-4::SL2::GFP + myo-2p::mCherry]. Polycistronic translational fusion; GFP and LIPL-4 expressed separately. Pick animals with red pharynx to maintain. Reference: Wang et al. Science. 2008 Nov 7;322(5903):957-60. |
| JIN1679 | jinEx10. | C. elegans | jinEx10 [hlh-30p::hlh-30::GFP + rol-6(su1006)]. Pick Rollers to maintain. hlh-30::GFP expression should be visible at relatively low magnification. Reference: Lapierre LR, et. al. Nat Commun. 2013 Aug 8;4:2267. |
| MAH132 | rrf-1(pk1417) I; unc-119(e2498::Tc1) III; wIs51 V. | C. elegans | wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Reference: Kumsta C, Hansen M. PLoS One. 2012;7(5):e35428. |
| MAH133 | rrf-1(pk1417) I; qIs19 V. | C. elegans | qIs19 [lag-2p::GFP::unc-54 3'UTR + rol-6(su1006)] V. Reference: Kumsta C, Hansen M. PLoS One. 2012;7(5):e35428. |
| MAH14 | daf-2(e1370) III; adIs2122. | C. elegans | adIs2122 [lgg-1::GFP + rol-6(su1006)]. Rollers. Maintain at 15C. ~30% animals form dauers at 20C. Not all animals will roll, but all should be GFP+. Reference: Egan DF, et al. Science. 2011 Jan 28;331(6016):456-61. |
| MAH145 | sqEx4. | C. elegans | sqEx4 [atg-18p::atg-18::GFP + rol-6(su1006)]. Pick GFP+ Rollers to maintain. Reference: McQuary et. al., Cell Report. 2016 doi: 10.1016/j.celrep.2016.02.012. |
| MAH160 | sqEx9. | C. elegans | sqEx9 [argk-1p::argk-1::mCherry + rol-6(su1006)]. Pick mCherry+ Rollers to maintain. Reference: McQuary et. al., Cell Report. 2016 doi: 10.1016/j.celrep.2016.02.012. |
| MAH172 | argk-1(ok2973) V. | C. elegans | Reference: McQuary et. al., Cell Report. 2016 doi: 10.1016/j.celrep.2016.02.012. |
| MAH19 | rrf-1(pk1417) I; myo-3(st386) V; stEx30. | C. elegans | stEx30 [myo-3p::GFP::myo-3 + rol-6(su1006)]. Rollers. Pick GFP+ Rollers to maintain. Reference: Kumsta C, Hansen M. PLoS One. 2012;7(5):e35428. |
| MAH20 | daf-16(mu86) I; adIs2122. | C. elegans | adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
| MAH205 | argk-1(ok2993) V. | C. elegans | Reference: McQuary et. al., Cell Report. 2016 doi: 10.1016/j.celrep.2016.02.012. |
| MAH215 | sqIs11. | C. elegans | sqIs11 [lgg-1p::mCherry::GFP::lgg-1 + rol-6]. Rollers. Tandem-tagged autophagy reporter strain. |
| MAH22 | rrf-1(pk1417) I; adIs2122. | C. elegans | adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Reference: Kumsta C, Hansen M. PLoS One. 2012;7(5):e35428. |
| MAH23 | rrf-1(pk1417) I. | C. elegans | pk1417 outcrossed 4 times to N2. Reference: Kumsta C, Hansen M. PLoS One. 2012;7(5):e35428. |
| MAH235 | sqIs19. | C. elegans | sqIs19 [hlh-30p::hlh-30::GFP + rol-6(su1006)]. Rollers. Slightly longer lived at 20C. hlh-30::GFP expression should be visible at relatively low magnification. [NOTE: the array does not segregate 100% in original stock; pick GFP+ and check for correct segregation to maintain the array. New stock recevied at CGC June, 2016.] Reference: Lapierre LR, et. al. Nat Commun. 2013 Aug 8;4:2267. |
| MAH236 | sqIs13. | C. elegans | sqIs13 [lgg-1p::GFP::lgg-1 + odr-1p::RFP]. Derived from QU10. Reference: Lapierre LR, et. al. Nat Commun. 2013 Aug 8;4:2267. |
| MAH240 | sqIs17. | C. elegans | sqIs17 [hlh-30p::hlh-30::GFP + rol-6(su1006)]. Rollers. Slightly longer lived at 20C. hlh-30::GFP expression should be visible at relatively low magnification. Derived from JIN1679. Reference: Lapierre LR, et. al. Nat Commun. 2013 Aug 8;4:2267. |
| MAH242 | sqIs24. | C. elegans | sqIs24 [rgef-1p::GFP::lgg-1 + unc-122p::RFP]. Derived by injection and subsequent integration of plasmids pMH882 and pMH876. Reference: Gelino S, et al. PLoS Genet. 2016 Jul 14;12(7):e1006135. Erratum in: PLoS Genet. 2016 Aug;12(8):e1006271. |
| MAH247 | sqIs25. | C. elegans | sqIs25 [atg-18p::atg-18::GFP + rol-6(su1006)]. GFP+ Rollers. Derived by integration of sqEx4 in MAH145. Reference: McQuary et. al., Cell Report. 2016 doi: 10.1016/j.celrep.2016.02.012. |
| MAH264 | sqEx27. | C. elegans | sqEx27 [sur-5p::argk-1::GFP + rol-6(su1006)]. Pick GFP+ Rollers to maintain. Reference: McQuary et. al., Cell Report. 2016 doi: 10.1016/j.celrep.2016.02.012. |
| MAH28 | aak-2(ok524) X; adIs2122. | C. elegans | adIs2122 [lgg-1::GFP + rol-6(su1006)]. Rollers. Reference: Egan DF, et al. Science. 2011 Jan 28;331(6016):456-61. |
| MAH300 | sqIs32. | C. elegans | sqIs32 [sur-5p::argk-1::GFP + rol-6(su1006)]. GFP+ Rollers. Derived by integration of sqEx27 in MAH264. Reference: McQuary et. al., Cell Report. 2016 doi: 10.1016/j.celrep.2016.02.012. |
| MAH349 | sqIs35. | C. elegans | sqIs35 [sqst-1p::sqst-1::GFP + unc-122p::RFP]. Strain over-expresses GFP-tagged p62/SQST-1, a key autophagy substrate. Reference: Kumsta C, et al. Nat Commun. 2019 Dec 11;10(1):5648. |
| MAH44 | glp-1(e2141) III; adIs2122. | C. elegans | adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Maintain at 15C or 20C. Sterile at 25C. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
| MAH50 | daf-16(mu86) I; glp-1(e2141) III; adIs2122. | C. elegans | adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Maintain at 15C or 20C. Sterile at 25C. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
| MAH508 | sqEx67. | C. elegans | sqEx67 [rgef-1p::mCherry::GFP::lgg-1 + rol-6]. Rollers. Pick Rollers to maintain. |
| MAH54 | adIs2122; mgEx779. | C. elegans | adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. mgEx779 [lipl-4p::lipl-4::SL2::GFP + myo-2p::mCherry]. Pick RFP+ Rollers to maintain. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
| MAH547 | sqEx82. | C. elegans | sqEx82 [argk-1p::GFP + rol-6(su1006)]. Pick Rollers with GFP expression in nerve ring and posterior intestine to maintain. Reference: McQuary et. al., Cell Report. 2016 doi: 10.1016/j.celrep.2016.02.012. |
| MAH677 | sid-1(qt9) V; sqIs71. | C. elegans | sqIs71 [rgef-1p::GFP + rgef-1p::sid-1]. Pan-neuronal expression of sid-1 driven by the rgef-1 promoter. Expression remains neuron-specific until at least Day 10. Animals respond to feeding RNAi against gfp and neuronal genes snb-1 and unc-13. In contrast, animals are resistant to phenotypes induced by feeding RNAi constructs targeting non-neuronal genes pept-1, unc-112, bli-1 and die-1. Reference: Yang Y, et al. Nat Aging 2024 Jan 4. doi: 10.1038/s43587-023-00548-1. PMID: 38177330. MAH677 effectively replaces strain TU3401 sid-1(pk3321) uIs69 [pCFJ90 (myo-2p::mCherry) + unc-119p::sid-1] V., which was found to show RNAi response in non-neuronal tissues. |
| MAH68 | mes-1(bn7) X; adIs2122. | C. elegans | adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Maintain at 15C or 20C. Approx. 50% sterility at 25C. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
| MAH704 | sqIs56. | C. elegans | sqIs56 [rgef-1p::sqst-1::GFP + unc-122p::RFP]. Neuronal over-expression of GFP-tagged p62/SQST-1, a key autophagy substrate. Reference: Kumsta C, et al. Nat Commun. 2019 Dec 11;10(1):5648. |
| MAH74 | rrf-1(pk1417) I; unc-119(ed3) ruIs32 III. | C. elegans | ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Reference: Kumsta C, Hansen M. PLoS One. 2012;7(5):e35428. |
| MAH844 | sqEx146. | C. elegans | sqEx146 [sqst-1p::sqst-1 + rol-6]. Pick Rollers to maintain array. Strain over-expresses non-tagged p62/SQST-1, a key autophagy substrate. Reference: Kumsta C, et al. Nat Commun. 2019 Dec 11;10(1):5648. |
| MAH88 | glp-1(e2141) III; mgEx779. | C. elegans | mgEx779 [lipl-4p::lipl-4::SL2::GFP + myo-2p::mCherry]. Pick RFP+ to maintain. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
| MAH914 | sqst-1(syb764) IV. | C. elegans | Full CRISPR deletion of sqst-1 locus. Reference: Kumsta C, et al. Nat Commun. 2019 Dec 11;10(1):5648. |
| MAH97 | rrf-1(pk1417) I; muIs109. | C. elegans | muIs109 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Reference: Kumsta C, Hansen M. PLoS One. 2012;7(5):e35428. |
| MAH99 | rrf-1(pk1417) I; muIs84. | C. elegans | muIs84 [sod-3p::GFP + rol-6(su1006)]. Weak rollers. Reference: Kumsta C, Hansen M. PLoS One. 2012;7(5):e35428. |
| QU10 | izEx5. | C. elegans | izEx5 [lgg-1p::GFP::lgg-1 + odr-1p::RFP]. Pick RFP+/GFP+ animals to maintain. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
| QU11 | glp-1(e2141) III; izEx5. | C. elegans | izEx5 [lgg-1p::GFP::lgg-1 + odr-1p::RFP]. Maintain at 15C or 20C. Sterile at 25C. Pick RFP+/GFP+ animals to maintain. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
| QU13 | glp-1(bn18) III; izEx5. | C. elegans | izEx5 [lgg-1p::GFP::lgg-1 + odr-1p::RFP]. Maintain at 15C or 20C. Sterile at 25C. Pick RFP+/GFP+ animals to maintain. Reference: Lapierre LR, Curr Biol. 2011 Sep 27;21(18):1507-14. |
This laboratory hasn't submitted any alleles to the CGC.