Laboratory Information

NameMAD View on WormBase
Allele designationdq
InstitutionUniversity of Wisconsin, Madison WI, USA
Address 425 Henry Mall
Madison 53706
United States
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
MAD21 unc-119(ed3) III; dqIs3; ddIs26. C. elegans dqIs3 [pie-1p::dyn1B::GFP + unc-119(+)]. ddIs26 [pie-1::mCherry::par-6 + unc-119(+)]. Grow at 24 degrees.
MAD3 unc-119(ed3) III; dqIs3. C. elegans dqIs3 [pie-1p::dyn1B::GFP + unc-119(+)]. Grow at 25 degrees.
MAD36 unc-119(ed3) III; dqIs44. C. elegans dqIs44 [pie-1::mCherry::moe + unc-119(+)]. Can be maintained at room temperature; shift to 25C for RNAi and imaging. Strain does not do well when kept at 25C for multiple generations. Reference: Shivas JM & Skop AR. Mol Biol Cell. 2012 May;23(10):1917-27.
MAD63 dqSi1 II; unc-119(ed3) III. C. elegans dqSi1 [mex-5p::atx-2a(cDNA)::GFP::tbb-2 3’UTR + unc-119(+)] II. Made by injection into strain EG6699; insertion confirmed by sequencing. dqSi1 rescues atx-2(ne4297). Reference: Gnazzo MM, et al. Mol Biol Cell. 2016 Oct 15;27(20):3052-3064. (PMID: 27559134) Del Castillo U, et al. Traffic. 2019 Jun;20(6):436-447. (PMID: 30989774)
temp_name244 unc-119(ed3) III; ojIs27. ojIs27 [spd-1::GFP + unc-119(+)]. GFP lost.
WH204 unc-119(ed3) III; ojIs1. C. elegans ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain under normal conditions. Reference: Strome et al. (2001) Mol Biol Cell (6):1751-64.
WH220 unc-119(ed3) III; ojIs7. C. elegans ojIs7 [zyg-12A::GFP + unc-119(+)].
WH223 ojIs9. C. elegans ojIs9 [zyg-12(all)::GFP + unc-119(+)].
WH237 ojIs10. C. elegans ojIs10 [lis-1::GFP + unc-119(+)].
WH257 unc-119(ed3) III; ojIs5. C. elegans ojIs5 [pie-1p::GFP::dnc-1 + unc-119(+)]
WH258 unc-119(ed3) III; ojIs57. C. elegans ojIs57 [pie-1::GFP::dnc-2 + unc-119(+)]
WH259 unc-119(ed3) III; ojIs47. C. elegans ojIs47 [arp-1::GFP + unc-119(+)].
WH276 unc-119(ed3) III; ojIs13. C. elegans ojIs13 [zyg-12B/C::GFP + unc-119(+)].
WH279 unc-119(ed3) III; ojIs12. C. elegans ojIs12 [cyk-4::GFP + unc-119(+)]. Strain is not very healthy. Described as integratred array but appears to segregate Uncs. Maintain at 15-20C.
WH280 unc-119(ed3) III; ojEx38. C. elegans ojEx38 [pie-1::GFP::cmd-1 + unc-119(+)]. Reference: Batchelder et al. (2007) FEBS Lett 581(22):4337-41.
WH342 unc-119(ed3) III; ojIs31. C. elegans ojIs31 [pie-1p::spd-3::GFP + unc-119(+)]. ojIs31 rescues spd-3(oj35) lethality at 25 C. Maintain at 25 C. Reference: Dinkelmann MV, et al., Genetics. 2007 Nov;177(3):1609-20.
WH347 unc-119(ed3) III; ojIs35. C. elegans ojIs35 [pie-1::GFP::rab-11.1 + unc-119(+)].
WH351 unc-119(ed3) III; ojIs37. C. elegans ojIs37 [pie-1p::GFP::ugtp-1 + unc-119(+)]. Maintain under normal conditions. Reference: Bembenek J, et al. Development. (2007) 134(21):3837-48.
WH497 unc-119(ed3) III; ojIs69. C. elegans ojIs69 [pie-1::mGFP::chin-1 + unc-119(+)]. N-terminal fusion of mGFP to sole predicted BE0003N10.2 ORF. Reference: Kumfer et al. (2010) Mol Biol Cell (2):266-77.
WH517 ojIs40. C. elegans ojIs40 [pie-1::mGFP::wsp-1(G-protein binding domain) + unc-119(+)]. Reference: Kumfer et al. (2010) Mol Biol Cell (2):266-77.
This laboratory hasn't submitted any alleles to the CGC.