Laboratory Information

NameMAD View on WormBase
Allele designationdq
HeadAHNA Renee SKOP
InstitutionUniversity of Wisconsin, Madison WI, USA
Address 425 Henry Mall
Madison 53706
United States
Website http://skoplab.weebly.com
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
MAD21 unc-119(ed3) III; dqIs3; ddIs26. C. elegans dqIs3 [pie-1p::dyn1B::GFP + unc-119(+)]. ddIs26 [pie-1::mCherry::par-6 + unc-119(+)]. Grow at 24 degrees.
MAD3 unc-119(ed3) III; dqIs3. C. elegans dqIs3 [pie-1p::dyn1B::GFP + unc-119(+)]. Grow at 25 degrees.
MAD36 unc-119(ed3) III; dqIs44. C. elegans dqIs44 [pie-1::mCherry::moe + unc-119(+)]. Can be maintained at room temperature; shift to 25C for RNAi and imaging. Strain does not do well when kept at 25C for multiple generations. Reference: Shivas JM & Skop AR. Mol Biol Cell. 2012 May;23(10):1917-27.
MAD63 dqSi1 II; unc-119(ed3) III. C. elegans dqSi1 [mex-5p::atx-2a(cDNA)::GFP::tbb-2 3’UTR + unc-119(+)] II. Made by injection into strain EG6699; insertion confirmed by sequencing. dqSi1 rescues atx-2(ne4297). Reference: Gnazzo MM, et al. Mol Biol Cell. 2016 Oct 15;27(20):3052-3064. (PMID: 27559134) Del Castillo U, et al. Traffic. 2019 Jun;20(6):436-447. (PMID: 30989774)
temp_name244 unc-119(ed3) III; ojIs27. ojIs27 [spd-1::GFP + unc-119(+)]. GFP lost.
WH204 unc-119(ed3) III; ojIs1. C. elegans ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain under normal conditions. Reference: Strome et al. (2001) Mol Biol Cell (6):1751-64.
WH220 unc-119(ed3) III; ojIs7. C. elegans ojIs7 [zyg-12A::GFP + unc-119(+)].
WH223 ojIs9. C. elegans ojIs9 [zyg-12(all)::GFP + unc-119(+)].
WH237 ojIs10. C. elegans ojIs10 [lis-1::GFP + unc-119(+)].
WH257 unc-119(ed3) III; ojIs5. C. elegans ojIs5 [pie-1p::GFP::dnc-1 + unc-119(+)]
WH258 unc-119(ed3) III; ojIs57. C. elegans ojIs57 [pie-1::GFP::dnc-2 + unc-119(+)]
WH259 unc-119(ed3) III; ojIs47. C. elegans ojIs47 [arp-1::GFP + unc-119(+)].
WH276 unc-119(ed3) III; ojIs13. C. elegans ojIs13 [zyg-12B/C::GFP + unc-119(+)].
WH279 unc-119(ed3) III; ojIs12. C. elegans ojIs12 [cyk-4::GFP + unc-119(+)]. Strain is not very healthy. Described as integratred array but appears to segregate Uncs. Maintain at 15-20C.
WH280 unc-119(ed3) III; ojEx38. C. elegans ojEx38 [pie-1::GFP::cmd-1 + unc-119(+)]. Reference: Batchelder et al. (2007) FEBS Lett 581(22):4337-41.
WH342 unc-119(ed3) III; ojIs31. C. elegans ojIs31 [pie-1p::spd-3::GFP + unc-119(+)]. ojIs31 rescues spd-3(oj35) lethality at 25 C. Maintain at 25 C. Reference: Dinkelmann MV, et al., Genetics. 2007 Nov;177(3):1609-20.
WH347 unc-119(ed3) III; ojIs35. C. elegans ojIs35 [pie-1::GFP::rab-11.1 + unc-119(+)].
WH351 unc-119(ed3) III; ojIs37. C. elegans ojIs37 [pie-1p::GFP::ugtp-1 + unc-119(+)]. Maintain under normal conditions. Reference: Bembenek J, et al. Development. (2007) 134(21):3837-48.
WH497 unc-119(ed3) III; ojIs69. C. elegans ojIs69 [pie-1::mGFP::chin-1 + unc-119(+)]. N-terminal fusion of mGFP to sole predicted BE0003N10.2 ORF. Reference: Kumfer et al. (2010) Mol Biol Cell (2):266-77.
WH517 ojIs40. C. elegans ojIs40 [pie-1::mGFP::wsp-1(G-protein binding domain) + unc-119(+)]. Reference: Kumfer et al. (2010) Mol Biol Cell (2):266-77.
This laboratory hasn't submitted any alleles to the CGC.