Laboratory Information
Name | MAD View on WormBase |
---|---|
Allele designation | dq |
Head | AHNA Renee SKOP |
Institution | University of Wisconsin, Madison WI, USA |
Address | 425 Henry Mall Madison 53706 United States |
Website | http://skoplab.weebly.com |
Gene classes |
Strains contributed by this laboratory
Strain | Genotype | Species | Description |
---|---|---|---|
MAD21 | unc-119(ed3) III; dqIs3; ddIs26. | C. elegans | dqIs3 [pie-1p::dyn1B::GFP + unc-119(+)]. ddIs26 [pie-1::mCherry::par-6 + unc-119(+)]. Grow at 24 degrees. |
MAD3 | unc-119(ed3) III; dqIs3. | C. elegans | dqIs3 [pie-1p::dyn1B::GFP + unc-119(+)]. Grow at 25 degrees. |
MAD36 | unc-119(ed3) III; dqIs44. | C. elegans | dqIs44 [pie-1::mCherry::moe + unc-119(+)]. Can be maintained at room temperature; shift to 25C for RNAi and imaging. Strain does not do well when kept at 25C for multiple generations. Reference: Shivas JM & Skop AR. Mol Biol Cell. 2012 May;23(10):1917-27. |
MAD63 | dqSi1 II; unc-119(ed3) III. | C. elegans | dqSi1 [mex-5p::atx-2a(cDNA)::GFP::tbb-2 3’UTR + unc-119(+)] II. Made by injection into strain EG6699; insertion confirmed by sequencing. dqSi1 rescues atx-2(ne4297). Reference: Gnazzo MM, et al. Mol Biol Cell. 2016 Oct 15;27(20):3052-3064. (PMID: 27559134) Del Castillo U, et al. Traffic. 2019 Jun;20(6):436-447. (PMID: 30989774) |
WH204 | unc-119(ed3) III; ojIs1. | C. elegans | ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain under normal conditions. Reference: Strome et al. (2001) Mol Biol Cell (6):1751-64. |
WH220 | unc-119(ed3) III; ojIs7. | C. elegans | ojIs7 [zyg-12A::GFP + unc-119(+)]. |
WH223 | ojIs9. | C. elegans | ojIs9 [zyg-12(all)::GFP + unc-119(+)]. |
WH237 | ojIs10. | C. elegans | ojIs10 [lis-1::GFP + unc-119(+)]. |
WH257 | unc-119(ed3) III; ojIs5. | C. elegans | ojIs5 [pie-1p::GFP::dnc-1 + unc-119(+)] |
WH258 | unc-119(ed3) III; ojIs57. | C. elegans | ojIs57 [pie-1::GFP::dnc-2 + unc-119(+)] |
WH259 | unc-119(ed3) III; ojIs47. | C. elegans | ojIs47 [arp-1::GFP + unc-119(+)]. |
WH276 | unc-119(ed3) III; ojIs13. | C. elegans | ojIs13 [zyg-12B/C::GFP + unc-119(+)]. |
WH279 | unc-119(ed3) III; ojIs12. | C. elegans | ojIs12 [cyk-4::GFP + unc-119(+)]. Strain is not very healthy. Described as integratred array but appears to segregate Uncs. Maintain at 15-20C. |
WH280 | unc-119(ed3) III; ojEx38. | C. elegans | ojEx38 [pie-1::GFP::cmd-1 + unc-119(+)]. Reference: Batchelder et al. (2007) FEBS Lett 581(22):4337-41. |
WH342 | unc-119(ed3) III; ojIs31. | C. elegans | ojIs31 [pie-1p::spd-3::GFP + unc-119(+)]. ojIs31 rescues spd-3(oj35) lethality at 25 C. Maintain at 25 C. Reference: Dinkelmann MV, et al., Genetics. 2007 Nov;177(3):1609-20. |
WH347 | unc-119(ed3) III; ojIs35. | C. elegans | ojIs35 [pie-1::GFP::rab-11.1 + unc-119(+)]. |
WH351 | unc-119(ed3) III; ojIs37. | C. elegans | ojIs37 [pie-1p::GFP::ugtp-1 + unc-119(+)]. Maintain under normal conditions. Reference: Bembenek J, et al. Development. (2007) 134(21):3837-48. |
WH497 | unc-119(ed3) III; ojIs69. | C. elegans | ojIs69 [pie-1::mGFP::chin-1 + unc-119(+)]. N-terminal fusion of mGFP to sole predicted BE0003N10.2 ORF. Reference: Kumfer et al. (2010) Mol Biol Cell (2):266-77. |
WH517 | ojIs40. | C. elegans | ojIs40 [pie-1::mGFP::wsp-1(G-protein binding domain) + unc-119(+)]. Reference: Kumfer et al. (2010) Mol Biol Cell (2):266-77. |
This laboratory hasn't submitted any alleles to the CGC.