Laboratory Information

NameLX View on WormBase
Allele designationvs
HeadMichael Robert Koelle
InstitutionYale University, New Haven, CT
Address 333 Cedar St., SHM CE30
Dept. of Molecular Biophysics & Biochemistry
Yale University School of Medicine
New Haven 06520-8024
United States
Website http://info.med.yale.edu/mbb/koelle/
Gene classes kcc  red  rgs  rsbp  gbas 

Strains contributed by this laboratory

Strain Genotype Species Description
LX1270 rsbp-1(vs163) I. C. elegans vs163 is a 169 bp deletion removing exon 2 and causing frameshift.
LX1313 eat-16(tm761) I; egl-10(md176) V. C. elegans Egl. Aldicarb-resistant.
LX147 rgs-1(nr2017) III. C. elegans Very weak Egl. rgs-1=C05B7.7, a C. elegans RGS (Regulator of G protein Signaling) gene. nr2017 is a presumptive null allele. nr2017 is a 638 bp deletion of sequences whose limits are CGAGAAATTGTCAACACTAAC...GTTTGGAATGGTTTATCAGTT. The deleted material is replaced by the following 35 bp insertion: TATGTTTAAGTTAAGTTTATAGTTTAAGTTTAAAG.
LX160 rgs-2(vs17) X. C. elegans rgs-2=F16H9.1, a C. elegans RGS (Regulator of G protein Signaling) gene. vs17 is a presumptive null allele. vs17 is a 1136 bp deletion of sequences with limits: ATATATATATCTCATTACTGG...AATCAAGTGTAACACTAATAT. rgs-1;rgs-2 double mutants fail to rapidly turn on egg-laying behavior when fed after starvation.
LX242 rgs-3&heri-1(vs19) II. C. elegans Healthy and appears grossly WT. This allele is a 1563 bp deletion of sequences AATTGAGTAGACAAC....GTGTCTTAAATAT. Removes almost all of the 2nd RGS domain. Previously known as cec-9.
LX475 rgs-5(vs58) III. C. elegans
LX533 rgs-6(vs62) X. C. elegans Deletion removes 1465 bp. Removes start site and majority of RGS domain. Has beginning and end sequences AAAAAGATCGAATATCGGTTGT...TATGACGTAGCACAATATCAGG.
LX543 rgs-8.1(vs64) X. C. elegans 2446 bp deletion. Endpoints: CAAAGGGAAACTTCACGAGAAA...ATTGATTATTACTAACCAAAGT.
LX604 rgs-4(vs93) II. C. elegans 231 bp deletion removes all of exons 2 and 3 and throws the remaining portion of the proten (which contains the RGS domain) out of frame. Deletion endpoints are: GCAGCTCACGGAGCCCGGAGTT...CACCGTCGCCAAGACTTAGGTA.
LX606 rgs-9(vs95) X. C. elegans This deletion removes most of exon1, all of exon 2, and most of exon 3. It removes a good chunk of the protein region, including the RGS domain.
LX636 dop-1(vs101) X. C. elegans 167 bp deletion which takes out most of exon 3 and the first 42 bases of exon 4. The first 22 bp of the deletion are: TATGGCTGATATCCGCAGGAAT.
LX645 dop-1(vs100) X. C. elegans 328 bp deletion which completely removes exons 8 and 9. The first 22 bp deleted are: cgttagtcccccttttaaaatt.
LX658 mnDp33 (X;IV)/+ IV; unc-20(e112) rgs-7(vs92) X. C. elegans Heterozygotes are WT. Animals which have lost the duplication are Unc and homozygous for rgs-7. Animals which are homozygous for the duplication are dead. Unc is temperature sensitive. vs92 is a 361 bp deletion which removes the 3' splice site of exon 6, all of exon 7 and half of exon 8. All of the deleted region is within the RGS domain.
LX671 ocr-2(vs29) IV. C. elegans Lays 86% of its eggs at the 8-cell or earlier stage.
LX677 rgs-7(vs98)/unc-1(n496) lon-2(e678) X. C. elegans Heterozygotes are Unc but not Lon. n496 is dominant. Segregates LonUnc. vs98 homozygotes are non-Unc, non-Lon and are Mel (they give only dead eggs). Strain will break down due to recombination so check for the presence of vs98 by PCR every few generations. Received new stock Feb 2005.
LX702 dop-2(vs105) V. C. elegans 125 bp deletion. First 22 bp of deletion: AAGTATATTTTATTTTCAGGTA. Last 22 bp: GTGGCCATCATAGTTATGCCAT.
LX703 dop-3(vs106) X. C. elegans 292 bp deletion. First 22 bp are: ACTTCCGTATTCCTTCTACTAC. Last 22 bp are: CTTAGCAGTTTCTGATTTTCTG.
LX704 dop-2(vs105) V; dop-3(vs106) X. C. elegans
LX705 dop-1(vs100) dop-3(vs106) X. C. elegans
LX706 dop-2(vs105) V; dop-1(vs100) X. C. elegans
LX733 rgs-10&rgs-11(vs110) X. C. elegans This deletion removes sequence 5' to start of rgs-10 and may therefore remove promoter sequence for rgs-10&rgs-11.
LX734 dop-2(vs105) V; dop-1(vs100) dop-3(vs106) X. C. elegans
LX811 vsIs33 V; lin-15B&lin-15A(n765) X. C. elegans vsIs33 [dop-3::RFP] V.
LX831 vsIs33 V; lin-15B&lin-15A(n765) X; vsIs28. C. elegans vsIs28 [dop-1::GFP]. vsIs33 [dop-3::RFP] V. Integration sites not known.
LX845 ocr-2(ak47) IV; ocr-1(ok132) V. C. elegans Double mutants have reduced AWA gene expression. ok132 is a deletion and putative null allele. The boundaries of the deletion are: AGATTACTGATGCCATTGAACAAGTTCTCGTCA......TGATGTTTGAAAGGTGGAGT AGCAAAGAGA. ak47 is chemosensory, mechanosensory and osmosensory defective, and is a null allele.
LX929 vsIs48. C. elegans vsIs48 [unc-17::GFP]. GFP expressed in all cholinergic neurons.
LX950 ocr-4(vs137) IV. C. elegans Phenotypically WT. Deletion allele. A "T" is added also. The end points of the deletion are: TCGAACGTCAACAACATATTGCAAAT.....t.....TTGGAAAGGTAGGCTTACACTT TTTTTAA.
LX959 vsIs13 IV; lin-15B&lin-15A(n765) X. C. elegans vsIs13 [lin-11::pes-10::GFP + lin-15(+)]. Expresses GFP in all 6 VC neurons, as well as in the posterior intestine. This is also uncharacterized embryonic expression.
LX960 lin-15B&lin-15A(n765) X; vsIs97. C. elegans vsIs97 [tph-1p::DsRed2 + lin-15(+)]. DsRed2 expression in HSN, NSM, and associated processes. Additional background expression in tail and a pair of head neurons. NOTE: tph-1p::DsRed2 expression pattern is different than GFP expression driven by the same promoter [Koelle Lab].
LX963 lin-15B&lin-15A(n765) X; vsIs100. C. elegans vsIs100 [myo-3p::CFP + lin-15(+)]. CFP expression in VMs. Additional background expression seen as punctate nucleolar fluorescence along the ventral side of the worm (also present in wild-type).
LX967 lin-15B&lin-15A(n765) vsIs103 X. C. elegans vsIs103 [tph-1p::RFP + snb-1::GFP + lin-15(+)] X. RFP expression in HSN and NSM neurons.
LX975 vsIs13 IV; lin-15B&lin-15A(n765) X; vsIs97; vsIs100. C. elegans vsIs13 [pes-10p::GFP + lin-15(+) IV. GFP expression in six VC neurons and posterior intestine. vsIs97 [tph-1p::DsRed2 + lin-15(+)]. DsRed2 expression in HSN, NSM, and associated processes. Additional background expression in tail and a pair of head neurons. NOTE: tph-1p::DsRed2 expression pattern is different than GFP expression driven by the same promoter [Koelle Lab]. vsIs100 [myo-3p::CFP + lin-15(+)]. CFP expression in VMs. Additional background expression seen as punctate nucleolar fluorescence along the ventral side of the worm (also present in wild-type).
LX980 ocr-4(vs137) IV; ocr-1(ok132) V. C. elegans vs137 is a deletion allele. A "T" has been added. The endpoints of the deletion are: TCGAACGTCAACAACATATTGCAAAT.......T.......TTGGAAAGGTAGGCTTAC ACTTTTTTTAA. Double mutants have reduced AWA gene expression. ok132 is a deletion and putative null allele. The boundaries of the deletion are: AGATTACTGATGCCATTGAACAAGTTCTCGTCA......TGATGTTTGAAAGGTGGAGT AGCAAAGAGA.
LX981 ocr-4(vs137) ocr-2(ak47) IV. C. elegans ak47 is chemosensory, mechanosensory and osmosensory defective, and is a null allele. vs137 is a deletion allele. A "T" has been added. The endpoints of the deletion are: TCGAACGTCAACAACATATTGCAAAT.......T.......TTGGAAAGGTAGGCTTAC ACTTTTTTTAA.
LX982 ocr-4(vs137) ocr-2(ak47) IV; ocr-1(ok132) V. C. elegans Chemosensory, mechanosensory, and osmosensory defects.
LX990 lin-15B&lin-15A(n765) X; vsEx494. C. elegans vsEx494 [ocr-2p::GFP::ocr-2 3'utr + lin-15(+)]. Labels the cells that express ocr-2. Pick non-Muv and GFP+ to maintain.
LX993 lin-15B&lin-15A(n765) X; vsIs108. C. elegans vsIs108 [tph-1p::RFP + unc-13::GFP + lin-15(+)] X. RFP expression in HSN and NSM neurons.
LX999 kcc-2(vs132) IV. C. elegans vs132 suppresses egl-47(n1082) egg laying defects.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
vs163 Allele deletion
vs17 Allele
vs19 Allele deletion
vs58 Allele
vs62 Allele
vs64 Allele
vs93 Allele
vs95 Allele
vs101 Allele deletion
vs100 Allele deletion
vs92 Allele
vs29 Allele
vs98 Allele
vs105 Allele deletion
vs106 Allele deletion
vs110 Allele
vs137 Allele insertion
vs132 Allele substitution