Laboratory Information
| Name | LX View on WormBase |
|---|---|
| Allele designation | vs |
| Head | Michael Robert Koelle |
| Institution | Yale University, New Haven, CT |
| Address | 333 Cedar St., SHM CE30 Dept. of Molecular Biophysics & Biochemistry Yale University School of Medicine New Haven 06520-8024 United States |
| Website | http://info.med.yale.edu/mbb/koelle/ |
| Gene classes | kcc red rgs rsbp gbas |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| LX1270 | rsbp-1(vs163) I. | C. elegans | vs163 is a 169 bp deletion removing exon 2 and causing frameshift. |
| LX1313 | eat-16(tm761) I; egl-10(md176) V. | C. elegans | Egl. Aldicarb-resistant. |
| LX147 | rgs-1(nr2017) III. | C. elegans | Very weak Egl. rgs-1=C05B7.7, a C. elegans RGS (Regulator of G protein Signaling) gene. nr2017 is a presumptive null allele. nr2017 is a 638 bp deletion of sequences whose limits are CGAGAAATTGTCAACACTAAC...GTTTGGAATGGTTTATCAGTT. The deleted material is replaced by the following 35 bp insertion: TATGTTTAAGTTAAGTTTATAGTTTAAGTTTAAAG. |
| LX160 | rgs-2(vs17) X. | C. elegans | rgs-2=F16H9.1, a C. elegans RGS (Regulator of G protein Signaling) gene. vs17 is a presumptive null allele. vs17 is a 1136 bp deletion of sequences with limits: ATATATATATCTCATTACTGG...AATCAAGTGTAACACTAATAT. rgs-1;rgs-2 double mutants fail to rapidly turn on egg-laying behavior when fed after starvation. |
| LX2060 | vsSi32 unc-119(ed3) III. | C. elegans | vsSi32 [goa-1::GFP + unc-119(+)] III. GOA-1::GFP translational fusion driven by 5kb of goa-1 promoter. Rescues unc-119 locomotion defect. vsSi32 is inserted into the left arm of chromosome III between sequences 5’-tttactgcatactgaacaacaggggaaaagggg-3’ and 5’-tagaattagctgtaagacggcgtctaggttttgca-3’. Reference: Kumar S, et al. G3 (Bethesda). 2021 Aug 7;11(8):jkab167. doi: 10.1093/g3journal/jkab167. PMID: 34003969 |
| LX2071 | goa-1(sa734) I; vsSi32 III. | C. elegans | vsSi32 [goa-1::GFP + unc-119(+)] III. GOA-1::GFP translational fusion driven by 5kb of goa-1 promoter. Rescues locomotion and body morphology defects, and partially rescues egg-laying defects seen in goa-1 null mutants. vsSi32 is inserted into the left arm of chromosome III between sequences 5’-tttactgcatactgaacaacaggggaaaagggg-3’ and 5’-tagaattagctgtaagacggcgtctaggttttgca-3’. Reference: Kumar S, et al. G3 (Bethesda). 2021 Aug 7;11(8):jkab167. doi: 10.1093/g3journal/jkab167. PMID: 34003969 |
| LX2404 | vsSi39 II; unc-119(ed3) III. | C. elegans | vsSi39 [goa-1-gfp(Q205L) + unc-119(+)] II. Single-copy mosSCI insertion of the gain-of-function goa-1(Q205L)::GFP translational fusion driven by 5kb of the goa-1 promoter. Animals have shallow body bends and are egg-laying defective. Reference: Kumar S, et al. G3 (Bethesda). 2021 Aug 7;11(8):jkab167. doi: 10.1093/g3journal/jkab167. PMID: 34003969 |
| LX242 | rgs-3&heri-1(vs19) II. | C. elegans | Healthy and appears grossly WT. This allele is a 1563 bp deletion of sequences AATTGAGTAGACAAC....GTGTCTTAAATAT. Removes almost all of the 2nd RGS domain. Previously known as cec-9. |
| LX2462 | lin-15B&lin-15A(n765) X; vsIs264. | C. elegans | vsIs264 [F35H10.10::GFP + lin-15(+)]. F35H10.10::GFP translational fusion. Reference: Fernandez RW, et al. J Neurosci. 2020 Sep 23;40(39):7475-7488. doi: 10.1523/JNEUROSCI.1357-20.2020. PMID: 32847964 |
| LX2562 | mjIs15. | C. elegans | mjIs15 [dlg-1::mCherry + lin-15(+)]. [NOTE: the mjIs15 transgene has been incorrectly described in some publications. The correct description of mjIs15 is a dlg-1::mCherry fusion protein and lin-15 rescue construct.] Reference: Lehrbach NJ, et al. Nat Struct Mol Biol. 2009 Oct; 16(10): 1016–1020. doi: 10.1038/nsmb.1675 PMID: 19713957 |
| LX475 | rgs-5(vs58) III. | C. elegans | |
| LX533 | rgs-6(vs62) X. | C. elegans | Deletion removes 1465 bp. Removes start site and majority of RGS domain. Has beginning and end sequences AAAAAGATCGAATATCGGTTGT...TATGACGTAGCACAATATCAGG. |
| LX543 | rgs-8.1(vs64) X. | C. elegans | 2446 bp deletion. Endpoints: CAAAGGGAAACTTCACGAGAAA...ATTGATTATTACTAACCAAAGT. |
| LX604 | rgs-4(vs93) II. | C. elegans | 231 bp deletion removes all of exons 2 and 3 and throws the remaining portion of the proten (which contains the RGS domain) out of frame. Deletion endpoints are: GCAGCTCACGGAGCCCGGAGTT...CACCGTCGCCAAGACTTAGGTA. |
| LX606 | rgs-9(vs95) X. | C. elegans | This deletion removes most of exon1, all of exon 2, and most of exon 3. It removes a good chunk of the protein region, including the RGS domain. |
| LX636 | dop-1(vs101) X. | C. elegans | 167 bp deletion which takes out most of exon 3 and the first 42 bases of exon 4. The first 22 bp of the deletion are: TATGGCTGATATCCGCAGGAAT. |
| LX645 | dop-1(vs100) X. | C. elegans | 328 bp deletion which completely removes exons 8 and 9. The first 22 bp deleted are: cgttagtcccccttttaaaatt. |
| LX658 | mnDp33 (X;IV)/+ IV; unc-20(e112) rgs-7(vs92) X. | C. elegans | Heterozygotes are WT. Animals which have lost the duplication are Unc and homozygous for rgs-7. Animals which are homozygous for the duplication are dead. Unc is temperature sensitive. vs92 is a 361 bp deletion which removes the 3' splice site of exon 6, all of exon 7 and half of exon 8. All of the deleted region is within the RGS domain. |
| LX671 | ocr-2(vs29) IV. | C. elegans | Lays 86% of its eggs at the 8-cell or earlier stage. |
| LX677 | rgs-7(vs98)/unc-1(n496) lon-2(e678) X. | C. elegans | Heterozygotes are Unc but not Lon. n496 is dominant. Segregates LonUnc. vs98 homozygotes are non-Unc, non-Lon and are Mel (they give only dead eggs). Strain will break down due to recombination so check for the presence of vs98 by PCR every few generations. Received new stock Feb 2005. |
| LX702 | dop-2(vs105) V. | C. elegans | 125 bp deletion. First 22 bp of deletion: AAGTATATTTTATTTTCAGGTA. Last 22 bp: GTGGCCATCATAGTTATGCCAT. |
| LX703 | dop-3(vs106) X. | C. elegans | 292 bp deletion. First 22 bp are: ACTTCCGTATTCCTTCTACTAC. Last 22 bp are: CTTAGCAGTTTCTGATTTTCTG. |
| LX704 | dop-2(vs105) V; dop-3(vs106) X. | C. elegans | |
| LX705 | dop-1(vs100) dop-3(vs106) X. | C. elegans | |
| LX706 | dop-2(vs105) V; dop-1(vs100) X. | C. elegans | |
| LX733 | rgs-10&rgs-11(vs110) X. | C. elegans | This deletion removes sequence 5' to start of rgs-10 and may therefore remove promoter sequence for rgs-10&rgs-11. |
| LX734 | dop-2(vs105) V; dop-1(vs100) dop-3(vs106) X. | C. elegans | |
| LX811 | vsIs33 V; lin-15B&lin-15A(n765) X. | C. elegans | vsIs33 [dop-3::RFP] V. |
| LX831 | vsIs33 V; lin-15B&lin-15A(n765) X; vsIs28. | C. elegans | vsIs28 [dop-1::GFP]. vsIs33 [dop-3::RFP] V. Integration sites not known. |
| LX845 | ocr-2(ak47) IV; ocr-1(ok132) V. | C. elegans | Double mutants have reduced AWA gene expression. ok132 is a deletion and putative null allele. The boundaries of the deletion are: AGATTACTGATGCCATTGAACAAGTTCTCGTCA......TGATGTTTGAAAGGTGGAGT AGCAAAGAGA. ak47 is chemosensory, mechanosensory and osmosensory defective, and is a null allele. |
| LX929 | vsIs48. | C. elegans | vsIs48 [unc-17::GFP]. GFP expressed in all cholinergic neurons. |
| LX950 | ocr-4(vs137) IV. | C. elegans | Phenotypically WT. Deletion allele. A "T" is added also. The end points of the deletion are: TCGAACGTCAACAACATATTGCAAAT.....t.....TTGGAAAGGTAGGCTTACACTT TTTTTAA. |
| LX959 | vsIs13 IV; lin-15B&lin-15A(n765) X. | C. elegans | vsIs13 [lin-11::pes-10::GFP + lin-15(+)]. Expresses GFP in all 6 VC neurons, as well as in the posterior intestine. This is also uncharacterized embryonic expression. |
| LX960 | lin-15B&lin-15A(n765) X; vsIs97. | C. elegans | vsIs97 [tph-1p::DsRed2 + lin-15(+)]. DsRed2 expression in HSN, NSM, and associated processes. Additional background expression in tail and a pair of head neurons. NOTE: tph-1p::DsRed2 expression pattern is different than GFP expression driven by the same promoter [Koelle Lab]. |
| LX963 | lin-15B&lin-15A(n765) X; vsIs100. | C. elegans | vsIs100 [myo-3p::CFP + lin-15(+)]. CFP expression in VMs. Additional background expression seen as punctate nucleolar fluorescence along the ventral side of the worm (also present in wild-type). |
| LX967 | lin-15B&lin-15A(n765) vsIs103 X. | C. elegans | vsIs103 [tph-1p::RFP + snb-1::GFP + lin-15(+)] X. RFP expression in HSN and NSM neurons. |
| LX975 | vsIs13 IV; lin-15B&lin-15A(n765) X; vsIs97; vsIs100. | C. elegans | vsIs13 [lin-11::pes-10::GFP + lin-15(+)]. GFP expression in six VC neurons and posterior intestine. vsIs97 [tph-1p::DsRed2 + lin-15(+)]. DsRed2 expression in HSN, NSM, and associated processes. Additional background expression in tail and a pair of head neurons. NOTE: tph-1p::DsRed2 expression pattern is different than GFP expression driven by the same promoter [Koelle Lab]. vsIs100 [myo-3p::CFP + lin-15(+)]. CFP expression in VMs. Additional background expression seen as punctate nucleolar fluorescence along the ventral side of the worm (also present in wild-type). |
| LX980 | ocr-4(vs137) IV; ocr-1(ok132) V. | C. elegans | vs137 is a deletion allele. A "T" has been added. The endpoints of the deletion are: TCGAACGTCAACAACATATTGCAAAT.......T.......TTGGAAAGGTAGGCTTAC ACTTTTTTTAA. Double mutants have reduced AWA gene expression. ok132 is a deletion and putative null allele. The boundaries of the deletion are: AGATTACTGATGCCATTGAACAAGTTCTCGTCA......TGATGTTTGAAAGGTGGAGT AGCAAAGAGA. |
| LX981 | ocr-4(vs137) ocr-2(ak47) IV. | C. elegans | ak47 is chemosensory, mechanosensory and osmosensory defective, and is a null allele. vs137 is a deletion allele. A "T" has been added. The endpoints of the deletion are: TCGAACGTCAACAACATATTGCAAAT.......T.......TTGGAAAGGTAGGCTTAC ACTTTTTTTAA. |
| LX982 | ocr-4(vs137) ocr-2(ak47) IV; ocr-1(ok132) V. | C. elegans | Chemosensory, mechanosensory, and osmosensory defects. |
| LX990 | lin-15B&lin-15A(n765) X; vsEx494. | C. elegans | vsEx494 [ocr-2p::GFP::ocr-2 3'utr + lin-15(+)]. Labels the cells that express ocr-2. Pick non-Muv and GFP+ to maintain. |
| LX993 | lin-15B&lin-15A(n765) X; vsIs108. | C. elegans | vsIs108 [tph-1p::RFP + unc-13::GFP + lin-15(+)] X. RFP expression in HSN and NSM neurons. |
| LX999 | kcc-2(vs132) IV. | C. elegans | vs132 suppresses egl-47(n1082) egg laying defects. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| vs163 | Allele | deletion | |
| vs17 | Allele | ||
| vs19 | Allele | deletion | |
| vs58 | Allele | ||
| vs62 | Allele | ||
| vs64 | Allele | ||
| vs93 | Allele | ||
| vs95 | Allele | ||
| vs101 | Allele | deletion | |
| vs100 | Allele | deletion | |
| vs92 | Allele | ||
| vs29 | Allele | ||
| vs98 | Allele | ||
| vs105 | Allele | deletion | |
| vs106 | Allele | deletion | |
| vs110 | Allele | ||
| vs137 | Allele | insertion | |
| vs132 | Allele | substitution |