Laboratory Information
| Name | LW View on WormBase |
|---|---|
| Allele designation | jj |
| Head | Jun (Kelly) Liu |
| Institution | Cornell University, Ithaca NY, USA |
| Address | 441 Biotechnology Building Cornell University Ithaca 14853 United States |
| Website | http://courses.cit.cornell.edu/liulab/ |
| Gene classes | drag rcor snu susm |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| DF5097 | Rhabditella axei wild isolate. | Rhabditella axei | Formerly known as Rhabditis axei. Isolated by Elie S. Dolgin in 2006 from agricultural soil at Naivasha, Kenya (0º50'S 36º22'E); isolate number NV-SH-2. First frozen by Fitch laboratory 9/5/2006. Gonochoristic. Grows well on NGM plates and OP50 at 20-25C. Freezes easily with C. elegans protocols with 70% viability. DF5097 was verified to be R. axei by intercrossing it with DF5006 and observing that their resulting F1 and F2 hybrid offspring were healthy and fertile. At 33C, DF5097 can live up to three days, although they developmentally arrest as late larvae or young adults, fail to produce offspring, and eventually die; in contrast, DF5006 either dies or arrests as first-stage (L1) larvae. Returning DF5097 to 20C from 33C rescues them from developmental arrest and death, and allows them to become fertile adults; in contrast, when DF5006 L1 larvae are returned to 20C, they sometimes grow to later stage larvae, but never grow to fertile adults. Thus, DF5097 shows clearly superior resistance to heat over DF5006; it shows somewhat greater fertility than DF5098 (which also withstands 33C) after being returned to 20C. |
| DF5098 | Rhabditella axei wild isolate. | Rhabditella axei | Formerly known as Rhabditis axei. Isolated by Elie S. Dolgin in 2006 from fungus at Limuru, Kenya (1º05'S 36º39'E); isolate number LI-OM-B-1&4. First frozen by Fitch laboratory 9/5/2006. Gonochoristic. Grows well on NGM plates and OP50 at 20-25C. Freezes easily with C. elegans protocols with >20% viability. DF5098 was verified to be R. axei by intercrossing it with DF5006 and observing that their resulting F1 and F2 hybrid offspring were healthy and fertile. At 33C, DF5098 can live for up to three days, although they developmentally arrest as late larvae or young adults, fail to produce offspring, and eventually die; in contrast, DF5006 either dies or arrests as first-stage (L1) larvae. Returning DF5098 to 20C from 33C rescues them from developmental arrest and death, and allows them to become fertile adults; in contrast, when DF5006 L1 larvae are returned to 20C, they sometimes grow to later larvae, but never grow to fertile adults. Thus, DF5098 shows clearly superior resistance to heat over DF5006; it shows somewhat lower fertility than DF5097 (which also withstands 33C) after being returned to 20C. |
| LW1089 | unc-119(ed4) III; jjIs1089. | C. elegans | jjIs1089 [npp-1::GFP + unc-119(+)]. Reference: J Cell Sci. 2009 Jun 15;122(Pt 12):1970-8. |
| LW1288 | arIs37 I; sma-6(jj1) II; cup-5(ar465) III. | C. elegans | arIs37 [myo-3p::ssGFP + dpy-20(+)] I. Small body size and 6 coelomocytes. myo-3p::ssGFP is a secreted GFP that is taken up by coelomocytes. |
| LW227 | mls-2(cc615) X. | C. elegans | Defects in postembryonic M lineage: randomized cleavage orientations, reduced cell proliferation, cell fate transformation from CCs and BWMs to SMs. About 30% larval and adult lethality. |
| LW2286 | lon-2(e678) X; jjIs2277. | C. elegans | jjIs2277 [pCXT51(5*RLR::pes-10p(deleted)::GFP) + LiuFD61(mec-7p::RFP)]; integrated on LG I or IV. Reference: Tian et al. (2010) Development 137(14):2375-84. |
| LW2308 | dbl-1(wk70) V; jjIs2277. | C. elegans | jjIs2277 [pCXT51(5*RLR::pes-10p(deleted)::GFP) + LiuFD61(mec-7p::RFP)]; integrated on LG I or IV. Reference: Tian et al. (2010) Development 137(14):2375-84. |
| LW2367 | arIs37 I; sma-9(cc604) X. | C. elegans | arIs37 [myo-3p::ssGFP + dpy-20(+)] I. myo-3p::ssGFP is a secreted GFP that is taken up by coelomocytes. Reference: Tian et al. (2010) Development 137(14):2375-84. |
| LW2436 | jjIs2277. | C. elegans | jjIs2277 [pCXT51(5*RLR::pes-10p(deleted)::GFP) + LiuFD61(mec-7p::RFP)]; integrated on LG I or IV. Reference: Tian et al. (2010) Development 137(14):2375-84. |
| LW257 | fozi-1(cc609) cup-5(ar465) III; him-5(e1467) V. | C. elegans | Missing M-derived coelomocytes and 1-3 body wall muscles. Cells transformed to sex myoblast-like fates. |
| LW425 | mls-2(tm252) X. | C. elegans | Defects in postembryonic M lineage: over-proliferation, cell fate transformation. |
| LW478 | sma-9(tm572) X. | C. elegans | |
| LW5558 | sma-4(jj278) III. | C. elegans | sma-4(jj278) worms are small, and the mutation can suppress the sma-9(0) loss of M-derived coelomocyte defect. sma-4(jj278) is a true molecular null allele of sma-4; the 3,556 bp deletion (position: Chromosome III: 5,816,203….5,819,759) removes almost the entire coding region of sma-4. Reference: McKillop AN, et al. (2018). A new deletion allele of sma-4. microPublication Biology. https://doi.org/10.17912/Z4Z9-CE10. |
| LW557 | arIs37 I; fozi-1(cc607) cup-5(ar465) III. | C. elegans | arIs37 [myo-3p::ssGFP + dpy-20(+)] I. Missing M-derived coelomocytes and 1-3 body wall muscles. Cells transformed to sex myoblast-like fates. myo-3p::ssGFP is a secreted GFP that it taken up by coelomocytes. |
| LW614 | arIs37 I; sma-3(jj3) cup-5(ar465) III. | C. elegans | arIs37 [myo-3p::ssGFP + dpy-20(+)] I. Small body size and 6 coelomocytes. myo-3p::ssGFP is a secreted GFP that is taken up by coelomocytes. |
| LW697 | ccIs4810 X. | C. elegans | ccIs4810 [(pJKL380.4) lmn-1p::lmn-1::GFP::lmn-1 3'utr + (pMH86) dpy-20(+)] I. GFP-tagged LMN-1 is expressed in all somatic cells. Reference: Haithcock E, et al. (2005) PNAS 102:16690-5. |
| LW699 | jjIs699. | C. elegans | jjIs699 [lmn-1p::emr-1::GFP::unc-54 3'UTR + unc-119(+)]. Reference: Haithcock E., et al. PNAS. 2005 Nov 15;102(46):16690-5. |
| LW709 | jjIs709 I. | C. elegans | jjIs709 [(pDRNL1) + lmn-1p::GFP::lmn-1::unc-54 3'utr + (pMH86) dpy-20(+)] I. GFP-tagged LMN-1 is expressed in all somatic cells. Reference: Haithcock E, et al. (2005) PNAS 102:16690-5. |
| LW905 | lmn-1(tm1502) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm1502 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| jj1 | Allele | substitution | nonsense |
| jj3 | Allele | substitution | splice_site |