Laboratory Information

NameLV View on WormBase
Allele designationwc
HeadVenolia, Lee
InstitutionWilliams College, Williamstown, MA
Address Williams College 135 Stone Hill Road Williamstown, MA 01267

Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
LV13 unc-45(wc4)/unc-45(e286) III. C. elegans Maintain this strain at 15C so that you can score for dead eggs (3 fold stage). At 15C, both hets and e286 homozygotes move reasonably well. Place single animals on plates and allow them to lay eggs for a day; then remove the parent and score the plates the next day for dead eggs. At 25C, both hets and e286 homozygotes will be Unc and Egl; dead eggs will remain inside the parent worm.
LV15 unc-45(st601)/daf-7(e1372) par-2(it46) III. C. elegans st601 is a non-conditional two-fold arrest lethal. Maintain at 25C to see daf-7 par-2 segregants (Daf-7(+) escapers will be Par). WT heterozygotes segregate WT, DafPar, 1/4 lethal eggs and two-fold arrest hatchlings. [3/97: dauers are not giving dead eggs-they are giving more dauers. The par-2 mutation may be lost.]
LV18 unc-45(wc1) dpy-1(e1)/daf-7(e1372) par-2(it46) III. C. elegans Maintain at 25C. At 25C, heterozygotes are WT and segregate WT, Dauers (dauer escapers will be Par and give only dead eggs), and DpyUcs which arrest as dead eggs (range from twitching multicellulars to 3-folds that hatch). [There is a greater percentage of hatchlings when the mother is heterozygous (wc1 dpy-1/+). There may also be the possibility of near complete maternal rescue (near full-sized, sterile Dpys), but this has not been routinely observed in the balanced strain (as opposed to wc1 dpy-1/+).] Maintain by picking WT at 25C and scoring for correct segregation of progeny. [3/97: The dauers are not giving dead eggs-they are giving other dauers. Appears that the Par mutation is no longer present.]
LV19 unc-45(wc2) dpy-1(e1)/daf-7(e1372) par-2(it46) III. C. elegans Maintain at 25C. At 25C, heterozygotes are WT and segregate WT, Dauers (escapers will be Par and give only dead eggs) and DpyUncs which are dead eggs (a range of embryonic lethality from multicellular twitchers to 3-folds that do not hatch). Maintain by picking WT at 25C and scoring for correct segregation of progeny. [3/97: the dauers are giving dead eggs.]
temp_name26 wcDf1 dpy-1(e1)/daf-7(e1372) par-2(it46)III. never recovered correct het-removed 2/97
temp_name29 unc-45(st604) dpy-1(e1)/daf-7(e1372) par-2(it46)III. difficult to maitain-may have lost it46

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
wc4 Allele
wc1 Allele
wc2 Allele