Laboratory Information

NameLG View on WormBase
Allele designationge
HeadLeonard P Guarente
InstitutionMIT, Cambridge MA, USA
Address M.I.T.
Biology, 68-280
31 Ames St
Cambridge 02139
United States
Gene classes sir 

Strains contributed by this laboratory

Strain Genotype Species Description
LG269 geIs3 dyf-x(ge1) I. C. elegans geIs3 [sir-2.1(+) + rol-6(su1006)]. Rollers. This strain replaces LG100. Reference: Viswanathan M, Guarente L. Nature. 2011 Sep 21;477(7365):E1-2.
LG326 skn-1(zu169) IV; geIs7. C. elegans geIs7 [skn-1b::GFP]. Reference: Nature (2007) 447(7144):545-9.
LG331 lin-35(n745) I; geIs7. C. elegans geIs7 [skn-1b::GFP]. Reference: Nature (2007) 447(7144):545-9.
LG333 skn-1(zu135) IV; geIs7. C. elegans geIs7 [skn-1b::GFP + rol-6(su1006)]. Rollers. Reference: Nature (2007) 447(7144):545-9.
LG340 skn-1(zu135) IV/nT1 [qIs51] (IV;V); geEx1. C. elegans geEx1 [gpa-4p::skn-1b::GFP + rol-6(su1006)]. Rollers. Pick Rolling GFP+ and check for correct segregation of progeny to maintain. skn-1 heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP skn-1 homozygotes (early arrest). Homozygous nT1[qIs51] inviable. Reference: Nature (2007) 447(7144):545-9.
LG344 geIs8. C. elegans geIs8 [gpa-4p::skn-1b::GFP + rol-6(su1006)]. Reference: Nature (2007) 447(7144):545-9.
LG345 geIs9. C. elegans geIs9 [gpa-4p::skn-1b::GFP + rol-6(su1006)]. Reference: Nature (2007) 447(7144):545-9.
LG348 skn-1(zu135) IV/nT1 [qIs51] (IV;V); geIs9. C. elegans geIs9 [gpa-4p::skn-1b::GFP + rol-6(su1006)]. Rollers. Heterozygotes are rollers with pharyngeal GFP signal, and segregate GFP+ rollers, arrested nT1[qIs51] aneuploids, and non-GFP skn-1 homozygotes (early arrest). Homozygous nT1[qIs51] inviable. Pick GFP+ rollers and check for correct segregation of progeny to maintain. Reference: Bishop & Guarente, Nature (2007) 447(7144):545-9.
LG349 geIs10. C. elegans geIs10 [ges-1p(long)::skn-1c::GFP + rol-6(su1006)]. Reference: Nature (2007) 447(7144):545-9.
LG357 skn-1(zu135) IV/nT1 [qIs51] (IV;V); geIs10. C. elegans geIs10 [ges-1p(long)::skn-1c::GFP + rol-6(su1006)]. Rollers. Heterozygotes are rollers with pharyngeal GFP signal, and segregate GFP+ rollers, arrested nT1[qIs51] aneuploids, and non-GFP skn-1 homozygotes (early arrest). Homozygous nT1[qIs51] inviable. Pick GFP+ rollers and check for correct segregation of progeny to maintain. Reference: Bishop & Guarente, Nature (2007) 447(7144):545-9.
LG394 geIs3 I. C. elegans geIs3 [sir-2.1(+) + rol-6(su1006)]. Rollers. This strain contains the transgene from LG100 crossed away from a linked dyf mutaion. Reference: Reference: Viswanathan M, Guarente L. Nature. 2011 Sep 21;477(7365):E1-2.
LG398 geIs101. C. elegans geIs101 [rol-6(su1006)]. Rollers. Reference: Viswanathan M, Guarente L. Nature. 2011 Sep 21;477(7365):E1-2.
LG433 dyf-x(ge1) I. C. elegans Reference: Viswanathan M, Guarente L. Nature. 2011 Sep 21;477(7365):E1-2.
NL3908 unc-119(ed3) III; pkIs1641. C. elegans pkIs1641[unc-119(+)]. Non-Unc strain.
NL3909 unc-119(ed3) III; pkIs1642. C. elegans pkIs1642[unc-119(+) + R11A8.5(+) + sir-2.1(+)]. Non-Unc strain.
temp_name215 skn-1(zu135) IV/nT1[qIs51] (IV;V); geIs9. Might have lost balancer
temp_name216 skn-1(zu135) IVnT1[qIs51] (IV;V); geIs10. Might have lost balancer
temp_name226 geIs3. Wrong genotype; geIs3 not present, add'l background mutation
temp_name252 skn-1(zu135) IV/nT1[qIs51] (IV;V); geEx2. LG can't confirm genotype -- shoudl it roll or not?

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
ge1 Allele