Laboratory Information
| Name | LE View on WormBase |
|---|---|
| Allele designation | lq |
| Head | Erik A Lundquist |
| Institution | University of Kansas, Lawrence, KS |
| Address | 5049 Haworth Hall 1200 Sunnyside Avenue Lawrence 66045 United States |
| Website | http://www.people.ku.edu/~erikl/Lundquist_Lab/Home.html |
| Gene classes | swan tiam |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| CX3662 | lin-15B&lin-15A(n765) X; kyIs121. | C. elegans | kyIs121 [unc-115::GFP + lin-15(+)]; autosomal. Maintain under normal conditions. Reference: Lundquist E, et al. (1998) Neuron 21(2):385-92. |
| CX3709 | unc-115(ky275) X. | C. elegans | Unc. Lon. |
| LE1012 | ced-10(tm597)/dpy-13(e184) IV. | C. elegans | Heterozygotes are semi-Dpy and throw semi-Dpy, Dpy, and WT (which throw all dead eggs). |
| LE1090 | tiam-1(ok772) I; ced-10(n1993) lqIs3 IV. | C. elegans | lqIs3 [osm-6::GFP] IV. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011 (Submitted). |
| LE1112 | tiam-1(ok772) I; mig-2(mu28) lqIs2 X. | C. elegans | lqIs2 [osm-6::GFP + lin-15(+)]. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011. |
| LE1212 | lqIs3 IV; mig-15(rh148) X. | C. elegans | Unc. Egl. lqIs3[osm-6::GFP]. |
| LE1213 | lqIs3 IV; mig-15(rh80) X. | C. elegans | lqIs3 [osm-6::GFP] IV. Unc. Egl. |
| LE137 | unc-73(rh40) I. | C. elegans | 24% PDE axon guidance errors, 28% ectopic axons. Reference: Lundquist et al. Development. 2001 Nov;128(22):4475-88. |
| LE1836 | rack-1(tm2262) IV/nT1 [qIs51] (IV;V). | C. elegans | Heterozygotes are WT and GFP+. tm2262 are viable but sick with low brood size. nT1[qIs51] is probably homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Pick wild-type GFP+ animals and check for correct segregation of progeny. Reference: Demarco RS, Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215. |
| LE1837 | rack-1(tm2262) IV. | C. elegans | Sick. Low brood size. Reference: Demarco RS, Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215. |
| LE1872 | lqEx387. | C. elegans | lqEx387 [mig-15::GFP]. Pick GFP+ to maintain. Reference: Chapman JO, et al. Dev Biol. 2008 Dec 15;324(2):245-57. |
| LE1923 | fli-1(tm362) III/hT2 [bli-4(e937) let-?(q782)] (I;III). | C. elegans | Sterile. Gonad & rachis defects. Grow at 20 C. |
| LE2113 | lin-15B&lin-15A(n765) X; lqEx463. | C. elegans | lqEx463 [rack-1::GFP + lin-15(+)]. Pick non-Muv to maintain. Reference: Demarco RS, Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215. |
| LE2290 | lqIs126. | C. elegans | lqIs126 [rack-1::MYC + osm-6::GFP]. lqIs126 rescues sterility, gonadal distal tip cell migration defects, and axon pathfinding defects caused by rack-1(tm2262). Reference: Demarco RS, Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215. |
| LE2336 | lqIs128. | C. elegans | lqIs128 [unc-25p::MYR::unc-40(constitutively active)::GFP + unc-25p::GFP]. Contains a myristylated, constitutively-active form of UNC-40. Slightly Unc. Reference: Norris AD & Lundquist EA. Development. 2011 Oct;138(20):4433-42. |
| LE2418 | unc-73(rh40) tiam-1(ok772) I; lqIs2 X. | C. elegans | lqIs2 [osm-6::GFP + lin-15(+)]. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011. |
| LE247 | kyIs4 X; lqEx27. | C. elegans | lqEx27 [ced-10(constitutively active) + str-1::GFP]. Pick animals with str-1::GFP (GFP+ in AWB amphid neurons). kyIs4 [ceh-23p::unc-76::GFP + lin-15(+)] X. Reference: Lundquist EA, et al. Development. 2001;128(22):4475-88. |
| LE2492 | lqIs151. | C. elegans | lqIs151 [scm::unc-40::GFP + str-1::GFP]. |
| LE2513 | tiam-1(tm1556) I; ced-10(n1993) lqIs3 IV. | C. elegans | lqIs3 [osm-6::GFP] IV. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011. |
| LE2514 | tiam-1(tm1556) I; mig-2(mu28) lqIs2 X. | C. elegans | lqIs2 [osm-6::GFP + lin-15(+)]. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011. |
| LE2517 | tiam-1(tm1556) I; lqIs2 X. | C. elegans | lqIs2 [osm-6::GFP + lin-15(+)].Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011. |
| LE2531 | unc-40(n324) I; juIs76 II. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Reference: Norris AD & Lundquist EA. Development. 2011 Oct;138(20):4433-42. |
| LE2717 | unc-73(rh40) tiam-1(tm1556) I; lqIs2 X. | C. elegans | lqIs2 [osm-6::GFP + lin-15(+)]. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011. |
| LE272 | fli-1(ky535) III. | C. elegans | Gonad & rachis defects. Grow at 20 C. |
| LE2791 | lqIs170 X. | C. elegans | lqIs170 [F25B3.3p::vab-10(ABD)::GFP + ttx-3::RFP] X. Pan-neuronal GFP expression. Reference: Norris AD & Lundquist EA. Development. 2011 Oct;138(20):4433-42. |
| LE3078 | lqEx631. | C. elegans | lqEx631 [tiam-1::CFP + str-1::GFP]. GFP expression in AWB amphid neurons. CFP neural expression in the head and tail, the ventral cord commissural motorneurons, the mechanosensory neurons (ALMs, PLMs, AVM, PVM) and the CAN, PDE, and PVD neurons. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665. |
| LE309 | lqIs2 X. | C. elegans | lqIs2 [osm-6::GFP + lin-15(+)]. |
| LE3091 | juIs76 II; unc-6(e78) X. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Reference: Norris AD & Lundquist EA. Development. 2011 Oct;138(20):4433-42. |
| LE310 | lqIs3 IV. | C. elegans | lqIs3 [osm-6::GFP] IV. |
| LE3190 | tiam-1(tm1556) I; juIs76 II; mig-2(mu28) lqIs2 X. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs2 [osm-6::GFP] X. lqIs2 carries a PDE/amphid/phasmid marker linked to mig-2. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665. |
| LE3191 | tiam-1(tm1556) I; juIs76 II; ced-10(n1993) lqIs3 IV. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs3 [osm-6::GFP] IV. lqIs3 carries a PDE/amphid/phasmid marker linked to ced-10. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665. |
| LE3192 | tiam-1(ok772) I; juIs76 II; mig-2(mu28) lqIs2 X. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs2 [osm-6::GFP] X. lqIs2 carries a PDE/amphid/phasmid marker linked to mig-2. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665. |
| LE3193 | tiam-1(ok772) I; juIs76 II; ced-10(n1993) lqIs3 IV. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs3 [osm-6::GFP] IV. lqIs3 carries a PDE/amphid/phasmid marker linked to ced-10. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665. |
| LE332 | lin-15B&lin-15A(n765) lqIs10 X. | C. elegans | lqIs10 [ceh-10::GFP + lin-15(+)]. lin-15(n765) mutation not confirmed after outcrossing. |
| LE341 | ced-10(n1993) lqIs3 IV. | C. elegans | lqIs3 [osm-6::GFP] IV. Cell corpse engulfment defect. lqIs3 carries a PDE/amphid/phasmid marker linked to ced-10. Reference: Struckhoff EC, Lundquist EA. Development 2003, 130:693-704. |
| LE3791 | lqIs250. | C. elegans | lqIs250 [unc-5::GFP + gcy-32::CFP]. Reference: Norris AD, et al. Development. 2014 Nov;141(22):4395-405. |
| LE3845 | rdvIs1 III; egl-20(gk453010) IV; lqIs58 V. | C. elegans | rdvIs1 [egl-17p::Myri-mCherry::pie-1 3'UTR + egl-17p::mig-10::YFP::unc-54 3'UTR + egl-17p::mCherry-TEV-S::his-24 + rol-6(su1006)] III. Rollers, red fluorescence in vulvae. YFP cannot be detected. lqIs58 [gcy-32::CFP] V. Reference: Josephson MP, et al. PLoS One. 2016 Feb 10;11(2):e0148658. |
| LE3992 | rdvIs1 III; lqIs80 IV. | C. elegans | rdvIs1 [egl-17p::Myri-mCherry::pie-1 3'UTR + egl-17p::mig-10::YFP::unc-54 3'UTR + egl-17p::mCherry-TEV-S::his-24 + rol-6(su1006)] III. Rollers, red fluorescence in vulvae. YFP cannot be detected. lqIs80 [SCMp::GFP::caax] IV. |
| LE436 | swan-1(ok267) V. | C. elegans | F53C11.8. Homozygous. Outer Left Sequence: AGGCGGAGAAAGTGACTTGA. Outer Right Sequence: CCCCTCACGCAGTGTTTTAT. Inner Left Sequence: TGAAGCAAATTGCAATCCAG. Inner Right Sequence: AACGAAATTGTGATCGGAGG. Inner Primer WT PCR Product: 2317. Deletion size: 1190 bp. |
| LE479 | juIs76 II; ced-10(n1993) lqIs3 IV. | C. elegans | juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs3 [osm-6::GFP] IV. Cell engulfment defect at 2-fold stage. Reference: Demarco RS & Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215. |
| LE554 | mig-2(mu28) lqIs2 X. | C. elegans | lqIs2 [osm-6::GFP] X. lqIs2 carries a PDE/amphid/phasmid marker linked to mig-2. Reference: Struckhoff EC, Lundquist EA. Development 2003, 130:693-704. |
| LE712 | ced-10(n1993) IV; lqEx245. | C. elegans | lqEx245 [ced-10::GFP + osm-6::GFP]. Pick GFP+ animals to maintain. lqEx245 rescues ced-10(n1993). |
| LE732 | lqIs27 IV; lin-15B&lin-15A(n765) X. | C. elegans | lqIs27 [ceh-23::GFP + lin-15(+)]. |
| LE815 | mig-2(lq13) X. | C. elegans | |
| LE948 | ced-10(n1993lq20) IV. | C. elegans | |
| LE983 | unc-34(lq17) V. | C. elegans | |
| MT10865 | unc-76(e911) V; nEx1039. | C. elegans | nEx1039 contains [ced-10p::GFP::ced-10 + unc-76(+)]. Maintain at 20 C. Described in Lunquist et al., Development 128, 4475-4488 (2001). |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| lq13 | Allele | substitution | |
| lq20 | Allele | substitution | |
| lq17 | Allele | substitution | splice_site |