Laboratory Information

NameLE View on WormBase
Allele designationlq
HeadErik A Lundquist
InstitutionUniversity of Kansas, Lawrence, KS
Address 5049 Haworth Hall
1200 Sunnyside Avenue
Lawrence 66045
United States
Website http://www.people.ku.edu/~erikl/Lundquist_Lab/Home.html
Gene classes swan  tiam 

Strains contributed by this laboratory

Strain Genotype Species Description
CX3662 lin-15B&lin-15A(n765) X; kyIs121. C. elegans kyIs121 [unc-115::GFP + lin-15(+)]; autosomal. Maintain under normal conditions. Reference: Lundquist E, et al. (1998) Neuron 21(2):385-92.
CX3709 unc-115(ky275) X. C. elegans Unc. Lon.
LE1012 ced-10(tm597)/dpy-13(e184) IV. C. elegans Heterozygotes are semi-Dpy and throw semi-Dpy, Dpy, and WT (which throw all dead eggs).
LE1090 tiam-1(ok772) I; ced-10(n1993) lqIs3 IV. C. elegans lqIs3 [osm-6::GFP] IV. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011 (Submitted).
LE1112 tiam-1(ok772) I; mig-2(mu28) lqIs2 X. C. elegans lqIs2 [osm-6::GFP + lin-15(+)]. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011.
LE1212 lqIs3 IV; mig-15(rh148) X. C. elegans Unc. Egl. lqIs3[osm-6::GFP].
LE1213 lqIs3 IV; mig-15(rh80) X. C. elegans lqIs3 [osm-6::GFP] IV. Unc. Egl.
LE137 unc-73(rh40) I. C. elegans 24% PDE axon guidance errors, 28% ectopic axons. Reference: Lundquist et al. Development. 2001 Nov;128(22):4475-88.
LE1836 rack-1(tm2262) IV/nT1 [qIs51] (IV;V). C. elegans Heterozygotes are WT and GFP+. tm2262 are viable but sick with low brood size. nT1[qIs51] is probably homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Pick wild-type GFP+ animals and check for correct segregation of progeny. Reference: Demarco RS, Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215.
LE1837 rack-1(tm2262) IV. C. elegans Sick. Low brood size. Reference: Demarco RS, Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215.
LE1872 lqEx387. C. elegans lqEx387 [mig-15::GFP]. Pick GFP+ to maintain. Reference: Chapman JO, et al. Dev Biol. 2008 Dec 15;324(2):245-57.
LE1923 fli-1(tm362) III/hT2 [bli-4(e937) let-?(q782)] (I;III). C. elegans Sterile. Gonad & rachis defects. Grow at 20 C.
LE2113 lin-15B&lin-15A(n765) X; lqEx463. C. elegans lqEx463 [rack-1::GFP + lin-15(+)]. Pick non-Muv to maintain. Reference: Demarco RS, Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215.
LE2290 lqIs126. C. elegans lqIs126 [rack-1::MYC + osm-6::GFP]. lqIs126 rescues sterility, gonadal distal tip cell migration defects, and axon pathfinding defects caused by rack-1(tm2262). Reference: Demarco RS, Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215.
LE2336 lqIs128. C. elegans lqIs128 [unc-25p::MYR::unc-40(constitutively active)::GFP + unc-25p::GFP]. Contains a myristylated, constitutively-active form of UNC-40. Slightly Unc. Reference: Norris AD & Lundquist EA. Development. 2011 Oct;138(20):4433-42.
LE2418 unc-73(rh40) tiam-1(ok772) I; lqIs2 X. C. elegans lqIs2 [osm-6::GFP + lin-15(+)]. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011.
LE247 kyIs4 X; lqEx27. C. elegans lqEx27 [ced-10(constitutively active) + str-1::GFP]. Pick animals with str-1::GFP (GFP+ in AWB amphid neurons). kyIs4 [ceh-23p::unc-76::GFP + lin-15(+)] X. Reference: Lundquist EA, et al. Development. 2001;128(22):4475-88.
LE2492 lqIs151. C. elegans lqIs151 [scm::unc-40::GFP + str-1::GFP].
LE2513 tiam-1(tm1556) I; ced-10(n1993) lqIs3 IV. C. elegans lqIs3 [osm-6::GFP] IV. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011.
LE2514 tiam-1(tm1556) I; mig-2(mu28) lqIs2 X. C. elegans lqIs2 [osm-6::GFP + lin-15(+)]. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011.
LE2517 tiam-1(tm1556) I; lqIs2 X. C. elegans lqIs2 [osm-6::GFP + lin-15(+)].Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011.
LE2531 unc-40(n324) I; juIs76 II. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Reference: Norris AD & Lundquist EA. Development. 2011 Oct;138(20):4433-42.
LE2717 unc-73(rh40) tiam-1(tm1556) I; lqIs2 X. C. elegans lqIs2 [osm-6::GFP + lin-15(+)]. Maintain under normal conditions. Reference: Demarco RS, Struckhoff EC, Lundquist EA. PLoS Genetics 2011.
LE272 fli-1(ky535) III. C. elegans Gonad & rachis defects. Grow at 20 C.
LE2791 lqIs170 X. C. elegans lqIs170 [F25B3.3p::vab-10(ABD)::GFP + ttx-3::RFP] X. Pan-neuronal GFP expression. Reference: Norris AD & Lundquist EA. Development. 2011 Oct;138(20):4433-42.
LE3078 lqEx631. C. elegans lqEx631 [tiam-1::CFP + str-1::GFP]. GFP expression in AWB amphid neurons. CFP neural expression in the head and tail, the ventral cord commissural motorneurons, the mechanosensory neurons (ALMs, PLMs, AVM, PVM) and the CAN, PDE, and PVD neurons. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665.
LE309 lqIs2 X. C. elegans lqIs2 [osm-6::GFP + lin-15(+)].
LE3091 juIs76 II; unc-6(e78) X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Reference: Norris AD & Lundquist EA. Development. 2011 Oct;138(20):4433-42.
LE310 lqIs3 IV. C. elegans lqIs3 [osm-6::GFP] IV.
LE3190 tiam-1(tm1556) I; juIs76 II; mig-2(mu28) lqIs2 X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs2 [osm-6::GFP] X. lqIs2 carries a PDE/amphid/phasmid marker linked to mig-2. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665.
LE3191 tiam-1(tm1556) I; juIs76 II; ced-10(n1993) lqIs3 IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs3 [osm-6::GFP] IV. lqIs3 carries a PDE/amphid/phasmid marker linked to ced-10. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665.
LE3192 tiam-1(ok772) I; juIs76 II; mig-2(mu28) lqIs2 X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs2 [osm-6::GFP] X. lqIs2 carries a PDE/amphid/phasmid marker linked to mig-2. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665.
LE3193 tiam-1(ok772) I; juIs76 II; ced-10(n1993) lqIs3 IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs3 [osm-6::GFP] IV. lqIs3 carries a PDE/amphid/phasmid marker linked to ced-10. Reference: Demarco RS, et al. PLoS Genet. 2012;8(4):e1002665.
LE332 lin-15B&lin-15A(n765) lqIs10 X. C. elegans lqIs10 [ceh-10::GFP + lin-15(+)]. lin-15(n765) mutation not confirmed after outcrossing.
LE341 ced-10(n1993) lqIs3 IV. C. elegans lqIs3 [osm-6::GFP] IV. Cell corpse engulfment defect. lqIs3 carries a PDE/amphid/phasmid marker linked to ced-10. Reference: Struckhoff EC, Lundquist EA. Development 2003, 130:693-704.
LE3791 lqIs250. C. elegans lqIs250 [unc-5::GFP + gcy-32::CFP]. Reference: Norris AD, et al. Development. 2014 Nov;141(22):4395-405.
LE3845 rdvIs1 III; egl-20(gk453010) IV; lqIs58 V. C. elegans rdvIs1 [egl-17p::Myri-mCherry::pie-1 3'UTR + egl-17p::mig-10::YFP::unc-54 3'UTR + egl-17p::mCherry-TEV-S::his-24 + rol-6(su1006)] III. Rollers, red fluorescence in vulvae. YFP cannot be detected. lqIs58 [gcy-32::CFP] V. Reference: Josephson MP, et al. PLoS One. 2016 Feb 10;11(2):e0148658.
LE3992 rdvIs1 III; lqIs80 IV. C. elegans rdvIs1 [egl-17p::Myri-mCherry::pie-1 3'UTR + egl-17p::mig-10::YFP::unc-54 3'UTR + egl-17p::mCherry-TEV-S::his-24 + rol-6(su1006)] III. Rollers, red fluorescence in vulvae. YFP cannot be detected. lqIs80 [SCMp::GFP::caax] IV.
LE436 swan-1(ok267) V. C. elegans F53C11.8. Homozygous. Outer Left Sequence: AGGCGGAGAAAGTGACTTGA. Outer Right Sequence: CCCCTCACGCAGTGTTTTAT. Inner Left Sequence: TGAAGCAAATTGCAATCCAG. Inner Right Sequence: AACGAAATTGTGATCGGAGG. Inner Primer WT PCR Product: 2317. Deletion size: 1190 bp.
LE479 juIs76 II; ced-10(n1993) lqIs3 IV. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. lqIs3 [osm-6::GFP] IV. Cell engulfment defect at 2-fold stage. Reference: Demarco RS & Lundquist EA. PLoS Genet. 2010 Nov 18;6(11):e1001215.
LE554 mig-2(mu28) lqIs2 X. C. elegans lqIs2 [osm-6::GFP] X. lqIs2 carries a PDE/amphid/phasmid marker linked to mig-2. Reference: Struckhoff EC, Lundquist EA. Development 2003, 130:693-704.
LE712 ced-10(n1993) IV; lqEx245. C. elegans lqEx245 [ced-10::GFP + osm-6::GFP]. Pick GFP+ animals to maintain. lqEx245 rescues ced-10(n1993).
LE732 lqIs27 IV; lin-15B&lin-15A(n765) X. C. elegans lqIs27 [ceh-23::GFP + lin-15(+)].
LE815 mig-2(lq13) X. C. elegans
LE948 ced-10(n1993lq20) IV. C. elegans
LE983 unc-34(lq17) V. C. elegans
MT10865 unc-76(e911) V; nEx1039. C. elegans nEx1039 contains [ced-10p::GFP::ced-10 + unc-76(+)]. Maintain at 20 C. Described in Lunquist et al., Development 128, 4475-4488 (2001).

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
lq13 Allele substitution
lq20 Allele substitution
lq17 Allele substitution splice_site