Laboratory Information

NameLC View on WormBase
Allele designationpa
HeadCurtis Mitchell Loer
InstitutionUniversity of San Diego
Address University of San Diego
Dept. of Biology
5998 Alcala Park
San Diego 92110
United States
Website http://www.sandiego.edu/~cloer/
Gene classes agmo  bas  gfrp  pah  pcbd  ptps  qdpr  pcdb 

Strains contributed by this laboratory

Strain Genotype Species Description
CF250 cat-4(e1141) him-5(e1490) V. C. elegans Catecholamine abnormal. Throws males.
CF311 mab-5(e1239) egl-5(n945) III; him-5(e1490) V. C. elegans
LC105 unc-46(e177) dpy-11(e224) uIs69 V. C. elegans uIs69 [(pCFJ90) myo-2p::mCherry + unc-119p::sid-1]. Hypersensitive neuronal RNAi by feeding. Dpy, Unc. Maintain 15-20 degrees. [NOTE: uIs69 is closely linked and maps to the right of dpy-11. (C. Loer 08/2011)] Derived from (BC277 x N2 Male) x TU3401. Reference: Calixto et al. (2010) Nature Methods 7:554-9.
LC108 uIs69 V. C. elegans uIs69 [(pCFJ90) myo-2p::mCherry + unc-119p::sid-1]. Hypersensitive neuronal RNAi by feeding. Superficially wild-type. Maintain 15-20 degrees. [NOTE: uIs69 is closely linked and maps to the right of dpy-11. (C. Loer 08/2011)] Derived from (unc-46(e177) uIs69) x N2 Male. Reference: Calixto et al. (2010) Nature Methods 7:554-9.
LC141 him-8(e1489) IV; cat-4(e3015) V. C. elegans Reduction in serotonin and dopamine, most apparent in young larva; bleach hypersensitivity and cuticle fragility in adult, but less than null mutants. Derived by outcrossing CB7107 six times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53.
LC143 pcbd-1(tm5924) I. C. elegans Slight reduction in serotonin and dopamine, most apparent in L1 larva. Derived by outcrossing FX5924 four times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53.
LC144 agmo-1(e3016) III. C. elegans General chemical hypersensitivity (including bleach), fragile cuticle. Derived by outcrossing CB7014 five times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53.
LC33 bas-1(tm351) III. C. elegans Serotonin-deficient by anti-serotonin straining. Likely also dopamine-deficient (but not tested).
LC35 cat-4(ok342) V. C. elegans T21C9.2, F32G8.6. Serotonin-deficient by anti-serotonin staining. Bleach hypersensitive. Likely also dopamine-deficient (but not tested). Slightly sickly. External left primer: TTTCTTTTCTTGTTGCGCCT. External right primer: TCGAAAAAGTCTGCTTCGGT. Internal left primer: CGTCTTCCGTTTCTTTTTCG. Internal right primer: TCTTGGAATGTGGGATGTGA. Internal WT amplicon: 2497 bp. Deletion size: 2238 bp. Deletion left flank: CGACTAGATTGATTTCCTTCTGTCCCTTCA. Deletion right flank: CTTGACGGAACAACGCCTCGATCTGATCTC. This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: http://www.celeganskoconsortium.omrf.org.
LC37 dpy-10(e128) unc-4(e120) II. C. elegans Dpy Unc. Derived from DR103. Outcrossed 3 times.
LC73 pah-1(tm520) II. C. elegans Superficially WT. Cuticle slightly more resistant to insult than WT. ES1-ES0. Severe cuticle abnormalities in double mutant with bli-3(e767).
LC74 pah-1(ok687) II. C. elegans Superficially WT. Cuticle slightly more resistant to insult than WT. ES1-ES0. Severe cuticle abnormalities in double mutant with bli-3(e767).
LC80 ptps-1(tm1984) I. C. elegans Serotonin- and dopamine-deficient, tetrahydrobiopterin-deficient, general chemical hypersensitivity, cuticle fragility (rapid disintegration in alkaline bleach), male turning defective (phenotypes appear identical to cat-4 null mutants). Derived by outcrossing FX1984 five times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53.
LC81 cat-4(tm773) V. C. elegans Serotonin and dopamine-deficient, bleach hypersensitive, general chemical hypersensitivity, fragile cuticle. 652 bp deletion removes entire first exon. Derived by outcrossing FX773 five times to N2.
LC87 qdpr-1(tm2337) III. C. elegans Slight reduction in serotonin and dopamine, most apparent in L1 larva. Derived by outcrossing FX2337 two times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53.
LC90 qdpr-1(tm2373) III. C. elegans Slight reduction in serotonin and dopamine, most apparent in L1 larva. Derived by outcrossing FX2373 two times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53.
This laboratory hasn't submitted any alleles to the CGC.