Laboratory Information
| Name | LC View on WormBase |
|---|---|
| Allele designation | pa |
| Head | Curtis Mitchell Loer |
| Institution | University of San Diego |
| Address | University of San Diego Dept. of Biology 5998 Alcala Park San Diego 92110 United States |
| Website | http://www.sandiego.edu/~cloer/ |
| Gene classes | agmo bas gfrp pah pcbd ptps qdpr pcdb |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| CF250 | cat-4(e1141) him-5(e1490) V. | C. elegans | Catecholamine abnormal. Throws males. |
| CF311 | mab-5(e1239) egl-5(n945) III; him-5(e1490) V. | C. elegans | |
| LC105 | unc-46(e177) dpy-11(e224) uIs69 V. | C. elegans | uIs69 [(pCFJ90) myo-2p::mCherry + unc-119p::sid-1]. Hypersensitive neuronal RNAi by feeding. Dpy, Unc. Maintain 15-20 degrees. [NOTE: uIs69 is closely linked and maps to the right of dpy-11. (C. Loer 08/2011)] Derived from (BC277 x N2 Male) x TU3401. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
| LC108 | uIs69 V. | C. elegans | uIs69 [(pCFJ90) myo-2p::mCherry + unc-119p::sid-1]. Hypersensitive neuronal RNAi by feeding. Superficially wild-type. Maintain 15-20 degrees. [NOTE: uIs69 is closely linked and maps to the right of dpy-11. (C. Loer 08/2011)] Derived from (unc-46(e177) uIs69) x N2 Male. Reference: Calixto et al. (2010) Nature Methods 7:554-9. |
| LC141 | him-8(e1489) IV; cat-4(e3015) V. | C. elegans | Reduction in serotonin and dopamine, most apparent in young larva; bleach hypersensitivity and cuticle fragility in adult, but less than null mutants. Derived by outcrossing CB7107 six times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53. |
| LC143 | pcbd-1(tm5924) I. | C. elegans | Slight reduction in serotonin and dopamine, most apparent in L1 larva. Derived by outcrossing FX5924 four times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53. |
| LC144 | agmo-1(e3016) III. | C. elegans | General chemical hypersensitivity (including bleach), fragile cuticle. Derived by outcrossing CB7014 five times to N2. [NOTE: the e3016 mutation is TGG>TGA but it affects codon W124, not W130.] Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53. |
| LC33 | bas-1(tm351) III. | C. elegans | Serotonin-deficient by anti-serotonin straining. Likely also dopamine-deficient (but not tested). |
| LC35 | cat-4(ok342) V. | C. elegans | T21C9.2, F32G8.6. Serotonin-deficient by anti-serotonin staining. Bleach hypersensitive. Likely also dopamine-deficient (but not tested). Slightly sickly. External left primer: TTTCTTTTCTTGTTGCGCCT. External right primer: TCGAAAAAGTCTGCTTCGGT. Internal left primer: CGTCTTCCGTTTCTTTTTCG. Internal right primer: TCTTGGAATGTGGGATGTGA. Internal WT amplicon: 2497 bp. Deletion size: 2238 bp. Deletion left flank: CGACTAGATTGATTTCCTTCTGTCCCTTCA. Deletion right flank: CTTGACGGAACAACGCCTCGATCTGATCTC. This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: http://www.celeganskoconsortium.omrf.org. |
| LC37 | dpy-10(e128) unc-4(e120) II. | C. elegans | Dpy Unc. Derived from DR103. Outcrossed 3 times. |
| LC74 | pah-1(ok687) II. | C. elegans | Superficially WT. Cuticle slightly more resistant to insult than WT. ES1-ES0. Severe cuticle abnormalities in double mutant with bli-3(e767). |
| LC80 | ptps-1(tm1984) I. | C. elegans | Serotonin- and dopamine-deficient, tetrahydrobiopterin-deficient, general chemical hypersensitivity, cuticle fragility (rapid disintegration in alkaline bleach), male turning defective (phenotypes appear identical to cat-4 null mutants). Derived by outcrossing FX1984 five times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53. |
| LC81 | cat-4(tm773) V. | C. elegans | Serotonin and dopamine-deficient, bleach hypersensitive, general chemical hypersensitivity, fragile cuticle. 652 bp deletion removes entire first exon. Derived by outcrossing FX773 five times to N2. |
| LC82 | Propanagrolaimus sp. wild isolate. | Propanagrolaimus sp. | Propanagrolaimus sp. wild isolate. Can be maintained and frozen using C. elegans conditions. Reference: Schiffer PH, et al. Dev Genes Evol. 2014 Jun;224(3):183-8. doi: 10.1007/s00427-014-0471-2. PMID: 24849338. |
| LC87 | qdpr-1(tm2337) III. | C. elegans | Slight reduction in serotonin and dopamine, most apparent in L1 larva. Derived by outcrossing FX2337 two times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53. |
| LC90 | qdpr-1(tm2373) III. | C. elegans | Slight reduction in serotonin and dopamine, most apparent in L1 larva. Derived by outcrossing FX2373 two times to N2. Reference: Loer CM, et al. Genetics. 2015 May;200(1):237-53. |
This laboratory hasn't submitted any alleles to the CGC.