Laboratory Information
| Name | KW View on WormBase |
|---|---|
| Allele designation | ck |
| Head | Kelly, William |
| Institution | Emory University, Atlanta, GA |
| Address | Emory University Biology Dept 1510 Clifton Rd Atlanta 30322 United States |
| Website | http://www.biology.emory.edu/research/Kelly/ |
| Gene classes | ccnk |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| DR682 | dpy-13(e184) ama-1(m118m235) IV/nT1 [qIs51] (IV;V). | C. elegans | Temperature sensitive. Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead embryos, and Dpy. Dpy progeny arrest in mid-larval stages at >20C. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416.Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. |
| DR730 | dpy-13(e184) ama-1(m118m238) IV. | C. elegans | Dpy. Sensitive to alpha-amanitin. Temperature sensitive, maintain at 15C. Fertile at 20C. Emb at restricitve temperature (25C). Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. |
| DR731 | dpy-13(e184) ama-1(m118m251) IV. | C. elegans | Dpy. Sensitive to alpha-amanitin. Temperature sensitive, maintain at 15C. Fertile at 20C. Emb at restrictive temperature (25C). Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. |
| DR811 | dpy-13(e184) ama-1(m118m332) IV/nT1 [qIs51] (IV;V). | C. elegans | Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead embryos, and Dpy. Dpy progeny arrest in L1 stage at >20C. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416.Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. |
| DR877 | dpy-13(e184) ama-1(m118m367) IV/nT1 [qIs51] (IV;V). | C. elegans | Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead embryos, and Dpy. Dpy progeny arrest in L1 stage at >20C. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416.Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. |
| DR880 | dpy-13(e184) ama-1(m118m370) IV/nT1 [qIs51] (IV;V). | C. elegans | Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead embryos, and Dpy. Dpy progeny arrest in L1 stage at >20C. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416.Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. |
| DR892 | dpy-13(e184) ama-1(m118m396) IV/nT1 [qIs51] (IV;V). | C. elegans | Temperature sensitive. Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead embryos, and Dpy. Dpy progeny are maternal effect Emb at 20C and arrest in mid-larval stages at 25C. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416.Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. |
| DR966 | dpy-13(e184) ama-1(+) IV. | C. elegans | Dpy. Sensitive to alpha-amanitin. [NOTE: formerly described as ama-1(m118m370m414), it has been determined that m414 was reversion of m367 back to wild-type sequence.] Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. |
| DR976 | dpy-13(e184) ama-1(m118m370m417) IV. | C. elegans | Dpy. Sensitive to alpha-amanitin. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. |
| KW1973 | taf-6.2(ax701) unc-17(e113) IV. | C. elegans | Unc. Maintain at 15C. Temperature-sensitive Emb when L4 larvae are shifted to restrictive temperature (25C). L1 larvae shifted to restrictive temperature arrest and die as young larvae. Reference: Bowman EA, et al. Worm Breeder's Gazette 2011 18(4). |
| KW1975 | taf-6.2(ax514) unc-17(e113) IV. | C. elegans | Unc. Maintain at 15C. Temperature-sensitive Emb when L4 larvae are shifted to restrictive temperature (25C). L1 larvae shifted to restrictive temperature arrest and die as young larvae. Reference: Bowman EA, et al. Worm Breeder's Gazette 2011 18(4). |
| KW2088 | cdk-12(tm3846)/qC1 [dpy-19(e1259) glp-1(q339)] nIs281 III. | C. elegans | nIs281 [myo-2::RFP] integrated near qC1. Recombination between nIs281 and qC1 has been reported. Fails to complemement all markers on qC1. Heterozygotes are WT RFP+ and segregate WT RFP+, Dpy Sterile RFP+ , and tm3846 homozygotes (Emb). Reference: Bowman EA, et al. Development. (In Press). |
| KW2090 | cdk-9(tm2884) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Segregates WT green-glowing heterozygotes and non-glowing cdk-9 homozygotes (arrest as L1-L2 larvae). qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Reference: Bowman EA, et al. Development. (In Press). |
| KW2096 | ckSi2 II; unc-119(ed3) III. | C. elegans | ckSi2 [cit-1.1::FLAG + unc-119(+)] II. Reference: Bowman EA, et al. Development. (In Press). |
| KW2098 | ckSi3 II; unc-119(ed3) III. | C. elegans | ckSi3 [cit-1.2::FLAG + unc-119(+)] II. Reference: Bowman EA, et al. Development. (In Press). |
| KW2104 | ckSi5 II; unc-119(ed3) III. | C. elegans | ckSi5 [spt-5::GFP + unc-119(+)] II. Reference: Bowman EA, et al. Development. (In Press). |
| KW2112 | ckSi6 I; unc-119(ed3) III. | C. elegans | ckSi6 [cdk-12::GFP + unc-119(+)] I. Reference: Bowman EA, et al. Development. (In Press). |
| KW2115 | ckSi4 II; unc-119(ed3) III. | C. elegans | ckSi4 [cdk-9::mCherry + unc-119(+)] II. Reference: Bowman EA, et al. Development. (In Press). |
| KW2117 | ckSi10 II; unc-119(ed3) III. | C. elegans | ckSi10 [ccnk-1::FLAG + unc-119(+)] II. Reference: Bowman EA, et al. Development. (In Press). |
| KW2126 | ckSi6 I; cdk-12(tm3846) III. | C. elegans | ckSi6 [cdk-12::GFP + unc-119(+)] I. ckSi6 rescues cdk-12(tm3846). GFP expressed in all nuclei. Reference: Bowman EA, et al. Development. (In Press). |
| KW2140 | ckSi14 II; unc-119(ed3) III. | C. elegans | ckSi14 [cit-1.2::GFP + unc-119(+)] II. Reference: Bowman EA, et al. Development. (In Press). |
| KW2147 | ckSi15 II; unc-119(ed3) III. | C. elegans | ckSi15 [ccnk-1::GFP + unc-119(+)] II. Reference: Bowman EA, et al. Development. (In Press). |
| KW2157 | ckSi9 I; unc-119(ed3) III. | C. elegans | ckSi9 [cdk-12(D462N)::GFP + unc-119(+)] I. Reference: Bowman EA, et al. Development. (In Press). |
| KW2159 | ckSi12 II; unc-119(ed3) III. | C. elegans | ckSi12 [cdk-9(D235N)::mCherry + unc-119(+)] II. Reference: Bowman EA, et al. Development. (In Press). |
| KW2167 | ckSi13 II; unc-119(ed3) III. | C. elegans | ckSi13 [cdk-9::GFP + unc-119(+)] II. Reference: Bowman EA, et al. Development. (In Press). |
| KW2181 | cdk-9(tm2884) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); ckSi12 II. | C. elegans | ckSi12 [cdk-9(D235N)::mCherry + unc-119(+)] II. Segregates WT green-glowing heterozygotes and non-glowing cdk-9 homozygotes (arrest as L1-L2 larvae). qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Reference: Bowman EA, et al. Development. (In Press). |
| KW2183 | cdk-9(tm2884) I; ckSi4 II. | C. elegans | ckSi4 [cdk-9::mCherry + unc-119(+)] II. ckSi4 rescues cdk-9(tm2884). mCherry is expressed in all nuclei. Reference: Bowman EA, et al. Development. (In Press). |
| KW2185 | ckSi6 I; ckSi4 II; unc-119(ed3) III. | C. elegans | ckSi6 [cdk-12::GFP + unc-119(+)] I. ckSi6 rescues cdk-12(tm3846). ckSi4 [cdk-9::mCherry + unc-119(+)] II. ckSi4 rescues cdk-9(tm2884). GFP and mCherry is expressed in all nuclei. Reference: Bowman EA, et al. Development. (In Press). |
| KW2194 | ckSi17 I; unc-119(ed3) III. | C. elegans | ckSi17 [cdk-12::GFP::mex-5 3'UTR + unc-119(+)] I. GFP is expressed in all somatic nuclei; expressed in germline only at end of oogenesis. Reference: Bowman EA, et al. Development. (In Press). |
| KW2195 | ckSi20 II; unc-119(ed3) III. | C. elegans | ckSi20 [cdk-9::mCherry::mex-5 3'UTR + unc-119(+)] II. mCherry is expressed in all somatic nuclei; expressed in germline only at end of oogenesis. Reference: Bowman EA, et al. Development. (In Press). |
| KW2196 | ckSi21 II; unc-119(ed3) III. | C. elegans | ckSi21 [cdk-9::GFP::pal-1 3'UTR + unc-119(+)] II. GFP is expressed in all somatic nuclei; expressed in germline only at end of oogenesis. Reference: Bowman EA, et al. Development. (In Press). |
| KW2205 | cdk-9(tm2884) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); ckSi21 II. | C. elegans | ckSi21 [cdk-9::mCherry::pal-1 3'UTR + unc-119(+)] II. mCherry is expressed in all somatic nuclei; expressed in germline only at end of oogenesis. hT2 segregates WT green-glowing heterozygotes and non-GFP cdk-9 homozygotes (normally arrest as L1-L2 larvae; cdk-9 homozygotes carrying ckSi21 (GFP- mCherry+) are viable but sterile. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Reference: Bowman EA, et al. Development. (In Press). |
| KW2206 | ckSi26 I; unc-119(ed3) III. | C. elegans | ckSi26 [cdk-12::GFP::pal-1 3'UTR + unc-119(+)] I. GFP is expressed in all somatic nuclei; expressed in germline only at end of oogenesis. Reference: Bowman EA, et al. Development. (In Press). |
| KW2211 | ckSi26 I; cdk-12(ok3664)/qC1 [dpy-19(e1259) glp-1(q339) qIs26] III. | C. elegans | qIs26 [lag-2::GFP + rol-6(su1006)]. ckSi26 [cdk-12::GFP::pal-1 3'UTR + unc-119(+)] I. GFP is expressed in all somatic nuclei; expressed in germline only at end of oogenesis. Throws heterozygous Rollers, tm3846 homozygotes (Emb), and tm3846 homozygotes (arrest as L1-L2 larvae). qIs26 was integrated into qC1 and in the process made qC1 homozygous lethal. The distal tip cells are GFP+. qIs26 is an integration of qEx233. Reference: Bowman EA, et al. Development. (In Press). |
| KW2214 | ckSi6 I; cdk-12(ok3664). | C. elegans | ckSi6 [cdk-12::GFP + unc-119(+)] I. ckSi6 rescues cdk-12(ok3664). GFP expressed in all nuclei. Reference: Bowman EA, et al. Development. (In Press). |
| KW2237 | ckSi25 II; unc-119(ed3) III. | C. elegans | ckSi25 [cit-1.2::FLAG::ccnk-1 3'UTR + unc-119(+)] II. GFP is expressed in all somatic nuclei. Reference: Bowman EA, et al. Development. (In Press). |
This laboratory hasn't submitted any alleles to the CGC.