Laboratory Information
| Name | KP View on WormBase |
|---|---|
| Allele designation | nu |
| Head | Joshua M Kaplan |
| Institution | Massachusetts General Hospital, Boston, MA |
| Address | 185 CAMBRIDGE ST. SIMCHES 7 MASSACHUSETTS GENERAL HOSPITAL BOSTON 02114 United States |
| Website | http://genetics.mgh.harvard.edu/kaplanweb/ |
| Gene classes | acy eos fab fshr gska not sbt |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| KP1008 | egl-36(n728n398) X. | C. elegans | Reference: Elkes DA, et al. Neuron. 1997 Jul;19(1):165-74. |
| KP1097 | dgk-1(nu62) X. | C. elegans | Hyperactive locomotion and egg-laying. Hypersensitive to aldicarb. Suppresses goa-1(gf). Allelic to sag-1. |
| KP1148 | nuIs25. | C. elegans | nuIs25 [glr-1p::glr-1::GFP + lin-15(+)]. Reference: Rango C, et al. Cell. 1998 Sep 18;94(6):751-9. |
| KP1182 | acy-1(nu329) III. | C. elegans | No obvious phenotype on its own. Suppresses cell swelling and death caused by expression of a GTPase defective Gxs subunit expressed from the glr-1 promoter, semi-dominant. Strongest allele. |
| KP1287 | nuIs26 IV. | C. elegans | nuIs26 [cat-1::GFP] IV. nuIs26 contains a full length CAT-1::GFP fusion protein (with GFP fused at the predicted carboxy terminus of CAT-1). This transgene rescues the hyperactive locomotion defect of cat-1(e1111). This strain is slightly sluggish for locomotion and has a high degree of sterility (80%), both of which appear to be associated with the transgene. GFP expression in this strain is similar to the CAT expression pattern described by Duerr et al (1999) J Neurosci 19: 72-84. |
| KP2048 | ric-7(nu447) V. | C. elegans | Reference: Hao Y, et al. PLoS Genet. 2012 Jan;8(1):e1002464. |
| KP2342 | pkc-1(nu448) V. | C. elegans | |
| KP2932 | lin-23(nu412)/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | |
| KP3814 | nuIs152 II. | C. elegans | nuIs152 [unc-129p::GFP::snb-1 + ttx-3p::mRFP] II. |
| KP3947 | nuIs183. | C. elegans | nuIs183 [unc-129p::nlp-21::Venus + myo-2p::GFP]. [NOTE: nuIs183 was previously described as carrying myo-2p::NLS::GFP.] Reference: Sieburth D, et al. Nat Neurosci. 2007 Jan;10(1):49-57. |
| KP3948 | eri-1(mg366) IV; lin-15B(n744) X. | C. elegans | Enhanced RNAi. Temperature sensitive sterile at 25C. Grow at 15 to 20C. |
| KP3957 | nuIs190 X. | C. elegans | nuIs190 [unc-129p::ins-22::Venus + myo-2p::NLS::GFP] X. Pick GFP+ to maintain. Not all animals express GFP. Array might be prone to silencing or not homozygous. [NOTE: nuIs190 was previously described as carrying myo-2p::GFP without an NLS.] Reference: Ch'ng Q, Sieburth D, Kaplan JM. PLoS Genet. 2008 Nov;4(11):e1000283. |
| KP3972 | nuIs184 X. | C. elegans | nuIs184 [unc-129p::apt-4::GFP + myo-2p::GFP] X. |
| KP3991 | nuIs145 V. | C. elegans | nuIs145 [glr-1p::vps-4(dominant negative)] V. Reference: Chun DK, et al. Mol Biol Cell. 2008 Jul;19(7):2682-95. |
| KP4 | glr-1(n2461) III. | C. elegans | pka not-3. Loss of function allele. Defective in response to nose touch but not to osmotic repellents. |
| KP4211 | nuIs214 III. | C. elegans | nuIs214 [unc-129p::itsn-1::GFP + myo-2p::GFP] III. |
| KP5445 | nuIs165. | C. elegans | nuIs165 [unc-129p::unc-10::GFP + myo-2p::GFP] II. |
| KP5832 | unc-108(nu415) I. | C. elegans | Reference: Chun DK, et al. Mol Biol Cell. 2012; 19, 2682-95. |
| KP6200 | ckr-2(tm3082) III. | C. elegans | Reference: Hu Z, et al. Neuron. 2011 Jul 14;71(1):92-102. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| nu62 | Allele | substitution | nonsense |
| nu329 | Allele | substitution | splice_site |
| nu447 | Allele | substitution | |
| nu448 | Allele | substitution | nonsense |
| nu412 | Allele | ||
| nu415 | Allele | deletion | |
| nu268 | Allele | ||
| nu288 | Allele |