Laboratory Information

NameJT View on WormBase
Allele designationsa
HeadThomas, Jim
InstitutionUniversity of Washington, Seattle
Address Department of Genome Sciences Box 357360 University of Washington Seattle, WA 98195-7360

Website http://www.gs.washington.edu/
Gene classes aex  bath  dec  exp  fbxa  fbxb  fbxc  gcy  heh  hid  irk  klq  kqt  math 
ncr  npc  nrf  nspb  nspc  pbo  scd  shk  shl  shw  srt  twk 

Strains contributed by this laboratory

Strain Genotype Species Description
BC3303 egl-38(s1775) unc-22(s7) unc-31(e169) IV/nT1 (IV;V). C. elegans s1775 homozygotes die as embryos or L1 larvae. Heterozygous animals are WT and segregate WT, Twitcher lethals and dead eggs. nT1 appears to have a lethal mutation.
BC4923 egl-38(sy294) IV; him-5(e1490) V. C. elegans
JT10066 unc-1(e719) pdk-1(sa680) X. C. elegans Unc. Daf-c at 25C. Egl, Clumpy, Lon, low brood size. Dauers do not recover when moved to 15C in the presence of food. Daf-c, Lon, Clumpy and fertility defects can be rescued maternally. Maintain at 15C.
JT1058 hid-1(sa1058) X. C. elegans Hid. Aex. Dec-s. Unc.
JT10800 ncr-2(nr2023) III; ncr-1(nr2022) X. C. elegans Daf-c (partial dauer), vulval abnormalities, broken alae, gonadal migration defects, low brood size, short lifespan. May grow better at 15C. Strain is healthier when recovered from starved plates by chunking than when continuously propagated. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JT11323 heh-1(ok603) III. C. elegans Cholesterol concentration-dependent Daf-c. If cholesterol is omitted from NGM, animals form dauers (no dauers are formed on regular NGM). Temperature sensitive Daf-c; heh-1 animals more readily form dauers at 27C than WT animals. Partial dauer phenotype; dauers exhibit ale but do not fully constrict pharynges nor arrest germline proliferation. On low cholesterol, vulva protrudes and sometimes ruptures; animals exhibit egg-laying defects. Hypersensititve to progesterone in culture media.
JT191 daf-28(sa191) V. C. elegans Temperature sensitive dauer constitutive. Highly penetrant at 25C, much less so at 20C and 15C. The dauers recover almost immediately after temperature down shift, so one may work with synchronous broods. Semi-dominant: about 10% of heterozygotes form dauers.
JT200 unc-43(sa200) IV. C. elegans Defecation motor program executed twice each cycle period. Hyperactive loopy movement. Previously called dec-8(sa200).
JT23 aex-5(sa23) I. C. elegans aBoc and Exp defective. Constipated.
JT24 aex-6(sa24) I. C. elegans aBoc and Exp defective. Constipated.
JT248 scd-1(sa248) X. C. elegans
JT249 scd-2(sa249) V. C. elegans Responds poorly to dauer pheromone.
JT253 scd-3(sa253) I. C. elegans Responds poorly to dauer pheromone. Low brood size. Egl. Gonadal left/right reversal. Mab.
JT292 dec-11(sa292) IV. C. elegans Long defecation cycle period. Slightly slow growing and scrawny.
JT293 dec-9(sa293) IV. C. elegans Short defecation cycle period. Semi-dominant.
JT294 dec-10(sa294) X. C. elegans Short defecation cycle period. Dominant.
JT295 dec-12(sa295) I. C. elegans Long defecation cycle period. Slightly slow growing and scrawny.
JT296 dec-7(sa296) III. C. elegans Temperature sensitive. Short defecation cycle period at 20C, longer at 25C. Appears to feed normally.
JT3 aex-2(sa3) X. C. elegans Constipated. aBoc and Exp defective.
JT363 nrf-3(sa363) IV. C. elegans Nrf.
JT366 vhp-1(sa366) II. C. elegans Nrf. sa366 previously called nrf-2.
JT37 lag-2(sa37) V. C. elegans Semidominant suppressor of lin-12(d) alleles.
JT48 dec-1(sa48) X. C. elegans Constipated. Long defecation cycle in mid to late adults.
JT5 aex-3(sa5) X. C. elegans Constipated. aBoc and Exp defective.
JT513 nrf-5(sa513) V. C. elegans Nrf, Peg, accumulates yolk in pseudo-coelomic space, slow growing, partial embryonic lethality.
JT5132 +/eT1 III; exp-2(sa26)/eT1 [let-?(n886)] V. C. elegans Heterozygotes have jerky movement, are Exp defective, and are Egl (dominant). Homozygous exp-2 are recessive lethal. Homozygous eT1 are lethal also.
JT5205 lin-12(n950) III; lag-2(sa37) V. C. elegans lag-2(sa37) is a semi-dominant suppressor of lin-12(n302sd).
JT5210 lin-12(n302) III; emb-4(sa44) V. C. elegans emb-4(sa44) is a recessive suppressor of lin-12(n302sd).
JT524 nrf-1(sa524) III. C. elegans Moderate Nrf, gonad migration defective.
JT5244 aex-4(sa22) X. C. elegans Constipated. aBoc and Exp defective.
JT525 nrf-6(sa525) II. C. elegans Nrf, Peg, accumulates yolk in pseudo-coelomic space, slow growing, partial embryonic lethality.
JT5277 sel-8(sa54) lin-12(n302) III. C. elegans sel-8(sa54) is a recessive suppressor of lin-12(n302sd).
JT528 nrf-4(sa528) I. C. elegans Moderate Nrf. Produces pale eggs (Peg) but does not accumulate yolk in pseudo-coelomic space.
JT5283 lin-12(n302) III; sel-7(n1253) X. C. elegans sel-7(n1253) is a recessive suppressor of lin-12(n302sd).
JT5516 saDf1/lon-1(e185) III. C. elegans Heterozygotes are WT and segregate WT, Lon and dead eggs. Hets are slow growing and sick.
JT573 akt-1(sa573) V. C. elegans Hid. Bor.
JT6 exp-1(sa6) II. C. elegans Exp defective. Constipated. May contain a weak daf-2 mutation (sa753). See Ailion and Thomas in Genetics 165: 127-144 2003.
JT603 gpb-2(sa603) I. C. elegans Recessive, loss of function, early stop mutation within the coding sequence makes sa603 a likely null allele. Variable locomotion ranging from lethargic to hyperactive. Eat (pale, scrawny). Loopy movement - increased amplitude of locomotory wave-form (variable). Suppresses the enteric muscle contraction (EMC) defect, the lethargy and egg-laying defects of unc-43(n498).
JT609 eat-16(sa609) I. C. elegans Recessive. Loss of function. Hyperactive, lays early stage eggs. Suppresses the enteric muscle contraction (EMC) defect, the lethargy and the egg-laying defects of unc-43(n498).
JT6130 hsp-90(p673) V. C. elegans Recessive Daf-c mutation that also causes Odr and Che defects. Maintain at 15C. [The mec-1 mutation present in the original isolates has been eliminated.] Previously known as daf-21.
JT6428 exp-3(n2372) V. C. elegans Defective in enteric muscle contraction (expulsion) step of defecation motor program and egg-laying (severe bloating-Egl Type A: serotonin and imipramine resistant). Enteric and egg-laying muscle structures are normal. Semi-dominant for Egl defect, but not for constipation defect. n2372/+ : ME3. ES3: adult heterozygotes and homozygotes.
JT646 hid-3(sa646) I. C. elegans Hid. Insensitive to dauer-inducing pheromone.
JT666 hid-4(sa666) IV. C. elegans Hid.
JT6932 exp-4(n2373) IV. C. elegans Defective in enteric muscle contraction (expulsion) step of defecation motor program and egg-laying (severe bloating- Egl Type A). Enteric and egg-laying muscle birefringence normal. Semi-dominant: n2373/+ is moderately Egl but not constipated. ME3 : n2373/+. ES3: adult homozygotes. ES2: heterozygotes.
JT698 hid-2(sa698) X. C. elegans Hid.
JT699 aex-6(sa699) I. C. elegans Hid. Con. Egl. Lethargic. Moderately Ric.
JT7 pbo-1(sa7) III. C. elegans Constipated. pBoc weak. Very slow growing.
JT709 pdk-1(sa709) X. C. elegans Daf-c at 27C. Weakly Egl and Clumpy. Maintain at 15C.
JT711 hid-6(sa711) I. C. elegans Hid.
JT722 hid-1(sa722) X. C. elegans Hid. Aex. Dec-S. Unc.
JT725 hid-5(sa725) X. C. elegans Hid. Egl. Weakly Clumpy.
JT73 itr-1(sa73) IV. C. elegans Long defecation cycle period. Slightly slow growing and scrawny. Reduced brood size, especially at 25C. See also WBPaper00003693; previously called dec-4(sa73).
JT734 goa-1(sa734) I. C. elegans Recessive, early stop mutation within the coding sequence (C to T substitution in aa52) makes sa734 a likely null allele. May grow slightly better at 15C. Hyperactive, lays early stage eggs, increased amplitude of locomotort wave-form. Suppresses the lethargy and egg-laying defects of unc-43(n498). Reverses direction of locomotion more frequently than WT.
JT748 dgk-1(sa748) X. C. elegans Recessive. Loss of function. Hyperactive, lays early stage eggs. Suppresses both the lethargy and egg-laying defect of unc-43(n498).
JT8132 lin-49(sa470) IV; him-5(e1490) V. C. elegans Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938.
JT8193 lin-59(sa489) I; him-5(e1490) V. C. elegans Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938.
JT8724 hid-7(sa572) IV. C. elegans Hid.
JT89 dec-2(sa89) III. C. elegans Long defecation cycle period. Slightly slow growing and scrawny.
JT9 aex-1(sa9) I. C. elegans Constipated. aBoc and Exp defective.
JT9609 pdk-1(sa680) X. C. elegans Daf-c at 25C. Egl, Clumpy, Lon, low brood size. Dauers do not recover when moved to 15C in the presence of food. Daf-c, Lon, Clumpy and fertility defects can be rescued maternally. Maintain at 15C.
JT9819 unc-24(e138) lin-49(s1198) unc-22(s7) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Unc, lethal Twitchers and dead eggs. Twitchers arrest in early-mid larval development. See also WBPaper00003938.
MT2769 sup-17(n1258) I. C. elegans sup-17(n1258) is a recessive suppressor of lin-12(n302sd).
MT2982 sel-4(n1259) II; lin-12(n302) III. C. elegans sel-4(n1259) is a recessive suppressor of lin-12(n302sd).
MT3000 sel-5(n1254) lin-12(n302) III. C. elegans sel-5(n1254) is a recessive suppressor of lin-12(n302sd). n1254 is cold sensitive.
MT3571 osm-8(n1518) II. C. elegans Partially osmotic avoidance defective. Recessive. FITC filling normal.
MT3643 osm-11(n1604) X. C. elegans
MT3743 unc-84(e1410) osm-11(n1604) X. C. elegans

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
sa307 Allele deletion
sa26 Allele
sa734 Allele substitution nonsense
sa680 Allele substitution
sa935 Allele substitution
sa1058 Allele substitution
sa156 Allele substitution
sa191 Allele substitution
sa193 Allele
sa200 Allele
sa204 Allele
sa23 Allele
sa24 Allele
sa248 Allele
sa249 Allele substitution nonsense
sa253 Allele
sa292 Allele
sa293 Allele
sa294 Allele
sa295 Allele
sa296 Allele
sa3 Allele substitution
sa363 Allele
sa366 Allele
sa37 Allele
sa47 Allele substitution nonsense
sa48 Allele
sa5 Allele
sa513 Allele substitution nonsense
sa44 Allele substitution
sa38 Allele
sa45 Allele
sa524 Allele
sa22 Allele substitution splice_site
sa525 Allele substitution nonsense
sa54 Allele substitution nonsense
sa528 Allele
sa529 Allele substitution
sa573 Allele substitution
sa6 Allele substitution
sa603 Allele substitution nonsense
sa609 Allele substitution
sa646 Allele
sa666 Allele
sa698 Allele
sa699 Allele
sa7 Allele
sa709 Allele
sa711 Allele
sa722 Allele substitution
sa725 Allele
sa73 Allele substitution
sa748 Allele
sa470 Allele substitution
sa489 Allele substitution nonsense
sa572 Allele
sa89 Allele
sa9 Allele substitution
sa94 Allele substitution
sa469 Allele substitution
sa232 Allele
sa62 Allele substitution
sa580 Allele substitution
sa127 Allele substitution
sa119 Allele substitution nonsense