Laboratory Information
| Name | JQ View on WormBase |
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| Allele designation | iu |
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| Head | Nabeshima, Kentaro |
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| Institution | University of Michigan, Ann Arbor, MI |
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| Address |
Department of Cell and Developmental Biology
University of Michigan, Medical School
109 Zina Pitcher Place
3029 BSRB
Ann Arbor, MI 48109-2200
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| Website |
http://www-personal.umich.edu/~knabe/ |
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| Gene classes |
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Strains contributed by this laboratory
| Strain |
Genotype |
Species |
Description |
| AV221 |
unc-119(ed3) meT8 (III); meIs4 meT8 (IV); meIs1. |
C. elegans |
meIs1 [pie-1p::GFP::lacI + unc-119(+)]. meIs4 [lac-O + rol-6(su1006) + lacO] IV. Pick Rol worms to maintain. This strain throws both Rol and non-Rol worms, seemingly due to random silencing of rol-6(su1006) in the lacO array, meIs4. The strain expresses GFP::LacI in the gonad and embryos that is observed as foci (of lacO target) and nuclear haze. The expression level of GFP::LacI occasionally becomes low possibly due to random silencing of meIs1. If this happens, heat shock the strain at 25°C for 3 days, and pick a clone that exhibits bright GFP signals. Even at the highest expression level, GFP signal is too weak to detect with a fluorescent dissection microscope, and it is necessary to use a regular compound fluorescent microscope with an oil immersion 60X or 100X objective. The NA of the objective should be higher than 1.4. Reference: Bilgir C, et al. G3 (Bethesda). 2013 Mar 11. pii: g3.112.005165v1. |
This laboratory hasn't submitted any alleles to the CGC.
