Laboratory Information

NameJQ View on WormBase
Allele designationiu
HeadNabeshima, Kentaro
InstitutionUniversity of Michigan, Ann Arbor, MI
Address Department of Cell and Developmental Biology University of Michigan, Medical School 109 Zina Pitcher Place 3029 BSRB Ann Arbor, MI 48109-2200

Website http://www-personal.umich.edu/~knabe/
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
AV221 unc-119(ed3) meT8 (III); meIs4 meT8 (IV); meIs1. C. elegans meIs1 [pie-1p::GFP::lacI + unc-119(+)]. meIs4 [lac-O + rol-6(su1006) + lacO] IV. Pick Rol worms to maintain. This strain throws both Rol and non-Rol worms, seemingly due to random silencing of rol-6(su1006) in the lacO array, meIs4. The strain expresses GFP::LacI in the gonad and embryos that is observed as foci (of lacO target) and nuclear haze. The expression level of GFP::LacI occasionally becomes low possibly due to random silencing of meIs1. If this happens, heat shock the strain at 25°C for 3 days, and pick a clone that exhibits bright GFP signals. Even at the highest expression level, GFP signal is too weak to detect with a fluorescent dissection microscope, and it is necessary to use a regular compound fluorescent microscope with an oil immersion 60X or 100X objective. The NA of the objective should be higher than 1.4. Reference: Bilgir C, et al. G3 (Bethesda). 2013 Mar 11. pii: g3.112.005165v1.
This laboratory hasn't submitted any alleles to the CGC.