Laboratory Information

NameJJ View on WormBase
Allele designationzu
HeadJames R Priess
InstitutionFred Hutchinson Cancer Research Center, Seattle, WA
Address Fred Hutchinson Cancer Center
Room A3-123 Weintraub Building
1100 Fairview Ave North
Seattle 98109
United States
Website http://labs.fhcrc.org/priess/jim.html
Gene classes aph  apx  arg  bop  eel  hmp  hmr  mex  mom  pie  pop  skn 

Strains contributed by this laboratory

Strain Genotype Species Description
EU1 skn-1(zu67) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Maintain by picking Unc.
JJ1014 mex-3(zu155) dpy-5(e61)/hT1 I; pos-1(zu148) unc-42(e270)/hT1 V. C. elegans The double heterozygote is WT. WT will segregate WT, DpyUncs, mid-larval lethal (hT1 homozygotes) and dead eggs. The DpyUncs are homozygous for zu155 and zu148 and will segregate only dead embryos; the dead embryos have excess hypodermis and muscle and lack germ cells and intestine.
JJ1057 pop-1(zu189) dpy-5(e61)/hT1 I; him-5(e1490)/hT1 V. C. elegans Heterozygotes are WT and segregate WT, Dpys which give only dead eggs, mid-larval lethals (hT1 homozygotes), males and dead eggs. Mutation in pop-1 results in the MS blastomere adopting the fate of the E blastomere. pop-1 mutant embryos have twice the amount of wild type gut and only make anterior pharynx. him-5 is outside the region balanced by hT2.
JJ1079 hmr-1(zu389)/lin-11(n566) unc-75(e950) I. C. elegans Heterozygotes are WT and segregate WT, Hmr inviable embyros and Egl Unc. Hmr: Hammerhead - defective hypodermal enclosure, especially in anterior regions; approximately 2% of zu389 embryos enclose normally and are Hmp [Humpback: defective body elongation, abnormal bulges on dorsal side]. See also WBPaper00005031. Received new stock from Allison Lynch in the Hardin lab 3/2009.
JJ1129 elt-1(zu180) unc-43(e408)/unc-24(e138) dpy-20(e1282) IV. C. elegans Heterozygotes are WT and segregate WT, DpyUncs and dead eggs.
JJ1142 hmr-1(zu248) I; zuEx2. C. elegans zuEx2 [W02B9(cosmid) + rol-6(su1006). Maintain by picking Rollers.
JJ1237 mex-6(pk440) II. C. elegans Viable, fertile, apparently normal.
JJ1238 unc-30(e191) mex-5(zu199) IV/nT1 (IV;V). C. elegans Heterozygotes are WT. Embryos from unc-30 mex-5 homozygotes produce approximately the WT number of cells but do not undergo body morphogenesis and die without hatching.
JJ1244 mex-6(pk440) II; unc-30(e191) mex-5(zu199) IV/nT1 (IV;V). C. elegans Heterozygotes are WT. Embryos from mex-6; unc-30 mex-5 homozygotes produce approximately the WT number of cells but do not undergo body morphogenesis and die without hatching.
JJ1271 glo-1(zu391) X. C. elegans Lacks autofluorescent and birefringent gut granules.
JJ1440 unc-119(ed3) III; zuIs20. C. elegans zuIs20 [par-3p::par-3::ZF1::GFP + unc-119(+)]. GFP-tagged PAR-3 that is degraded in early embryonic somatic cells. Worms carrying the transgene are non-Unc and express GFP very weakly in early embryos (only detectable by antibody staining), and later in adherens junctions of epithelial cells.
JJ1473 unc-119(ed3) III; zuIs45 V. C. elegans zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. NMY-2::GFP is expressed in the germline and somatic gonad.
JJ1494 unc-119(ed3) III; zuIs58. C. elegans zuIs58 [unc-119(+) + par-6::PAR-6::ZF1::GFP].
JJ1508 unc-119(ed3) III; zuIs60. C. elegans zuIs60 [pie-1p::GFP(secreted) + unc-119(+)]. Maternally-expressed secreted GFP fills spaces between embryonic cells, and space between embryo and vitelline membrane. Useful marker for vizualizing intercellular spaces in embryos. Reference: Wehman AM, et al. Curr Biol. 2011 Dec 6;21(23):1951-9.
JJ1549 efl-1(se1) V. C. elegans Temperature sensitive. At restrictive temperature (26C), efl-1 produces dead eggs with a Mex phenotype. Two ts periods: 1) prior to L4 results in sterility, 2) after L4 results in a maternal-effect embryonic lethal phenotype.
JJ1550 dpl-1(zu355) unc-4(e120)/rol-6(e187) let-23(sy97) II. C. elegans Heterozygotes are WT and segregate WT, Uncs which give only deads egss with a Mex phenotype, and Vulvaless Rollers. sy97 is only 15% viable.
JJ1833 dpy-5(e61) I; unc-32(e189) III. C. elegans Dpy Unc.
JJ1850 unc-119(ed3) III; zuIs178. C. elegans zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. HIS-72::GFP can be detected in germline and most somatic nuclei, but not in intestinal nuclei. Not integrated on X.
JJ1851 unc-119(ed3) III; zuEx181. C. elegans zuEx181[his-72(1kb 5' UTR)::YFP::GSRPVAT::HIS-72::HIS-72 (1KB 3'UTR) + 5.7 kb XbaI - HindIII UNC-119(+)]. HIS-72::YFP signal can be detected in the germline and most somatic nuclei, but not in intestinal nuclei.
JJ1852 unc-119(ed3) III; zuEx182. C. elegans zuEx182[his-71(1kb 5' UTR):: HIS-71::GSRPVAT::GFP::::HIS-71 (1KB 3'UTR) + 5.7 kb XbaI - HindIII UNC-119(+)]. HIS-71::YFP signal can be detected in most somatic nuclei, including the intestinal nuclei, but is not detected in the germline.
JJ1972 eel-1(zu462) unc-33(e204) IV. C. elegans Slow growth and a maternal-effect enhancer of the efl-1(se1) embyronic lethal phenotype. Unc.
JJ1992 unc-119(ed3) III; zuIs145. C. elegans zuIs145 [nmy-2p::mom::GFP + unc-119(+)]. Wild type phenotype.
JJ2059 unc-119(ed3) III; zuIs235. C. elegans zuIs235 [his-72(1-kb 5' UTR):: BIOTAG::3XHA::HIS-72::his-72(1-kb 3' UTR) + unc-119(+)]. Superficially wild-type.
JJ2060 unc-119(ed3) III; zuIs236. C. elegans zuIs236 [his-72(1-kb 5' UTR)::BIRA::GFP:: his-72(1-kb 3' UTR) + unc-119(+)]. Superficially wild-type.
JJ2061 unc-119(ed3) III; zuIs235; zuIs236. C. elegans zuIs235 [his-72(1-kb 5' UTR):: BIOTAG::3XHA::HIS-72::his-72(1-kb 3' UTR) + unc-119(+)]. zuIs236 [his-72(1-kb 5' UTR)::BIRA::GFP:: his-72(1-kb 3' UTR) + unc-119(+)]. Superficially wild-type.
JJ2281 unc-119(ed3) III; zuIs258. C. elegans zuIs258 [his-72p::his-72(5' UTR)::BIRA::GFP::his-72(3' UTR) + unc-119(+)]. GFP expression detectable in embryos. Reference: Steiner FA, et al. Genome Res. 2012 Apr;22(4):766-77.
JJ2286 unc-119(ed3) III; zuIs263. C. elegans zuIs263 [myo-3p::myo-3(5' UTR)::npp-9::mCherry::BLRP::3xFLAG::npp-9(3' UTR) + unc-119(+)]. Reference: Steiner FA, et al. Genome Res. 2012 Apr;22(4):766-77.
JJ2300 unc-119(ed3) III; zuIs258; zuIs263. C. elegans zuIs258 [his-72p::his-72(5' UTR)::BIRA::GFP::his-72(3' UTR) + unc-119(+)]. GFP expression detectable in embryos. zuIs263 [myo-3p::myo-3(5' UTR)::npp-9::mCherry::BLRP::3xFLAG::npp-9(3' UTR) + unc-119(+)]. Reference: Steiner FA, et al. Genome Res. 2012 Apr;22(4):766-77.
JJ2586 cox-4(zu476[cox-4::eGFP::3xFLAG]) I. C. elegans cox-4(zu476[cox-4::eGFP::3xFLAG]) I. Endogenous cox-4 locus tagged with eGFP via genome editing. Mitochondria in all cell types are labeled with GFP. Reference: Raiders SA, et al. PLoS Genet. 2018 Jul 19;14(7):e1007417.
JJ462 +/nT1 IV; pos-1(zu148) unc-42(e270)/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Uncs, Vul and dead eggs. Uncs are homozygous for zu148 and will segregate only dead embryos; the dead embryos will have no morphogenesis and will lack germ cells and intestine.
JJ478 mex-3(zu155) egl-30(n686)/hT1 I; +/hT1 V. C. elegans Heterozygotes are WT and segregate WT, Egls which give only dead eggs, dead eggs, and mid-larval lethals (hT1 homozygotes)
JJ529 rol-1(e91) mex-1(zu121)/mnC1 [dpy-10(e128) unc-52(e444)] II. C. elegans Heterozygotes are WT and segregate WT, DpyUnc and Rollers. Rollers give only dead eggs. Maintain by picking WT. mex-1 is to the right of rol-1, is uncovered by mnDf87 and is allelic to mel-21.
JJ532 pie-1(zu154) unc-25(e156)/qC1 [dpy-19(e1259) glp-1(q339)] III. C. elegans Heterozygotes are WT and segregate WT, Unc and DpySteriles. Uncs give only dead eggs. Maintain by picking WT.
JJ746 +/nT1 IV; apx-1(zu183) dpy-11(e224)/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Dpy, Vul and dead eggs. The Dpys give only dead eggs. apx-1 is a maternal effect lethal. zu183 is recessive.
JJ862 hmp-1(zu278)/daf-11(m84) sma-1(e30) V. C. elegans Heterozygotes are WT and segregate WT, Hmp inviable embyros (Hmp: humpback-defective body elongation, abnormal bulges on dorsal side) and Daf Sma (ts Daf).
JJ938 unc-24(e138) daf-14(m77)/elt-1(zu180) dpy-20(e1282) IV. C. elegans At 20C or 25C, heterozygotes are WT and segregate WT, UncDaf and dead eggs. At 15C, heterozygotes are WT and segregate WT, Uncs and dead eggs.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
zu181 Transposon insertion insertion
zu67 Allele substitution nonsense
zu154 Allele
zu135 Allele substitution nonsense
zu169 Allele substitution nonsense
zu129 Allele substitution nonsense
zu193 Allele
zu170 Transposon insertion insertion
zu446 Allele substitution nonsense
zu456 Allele substitution nonsense
zu310 Allele substitution
zu155 Allele
zu148 Allele substitution nonsense
zu189 Allele
zu364 Allele substitution
zu389 Allele substitution nonsense
zu180 Allele substitution
zu248 Allele
zu199 Allele
zu391 Allele substitution splice_site
zu355 Allele
zu462 Allele insertion
zu121 Allele
zu183 Allele
zu278 Allele substitution nonsense
zu222 Transposon insertion insertion
zu225 Allele substitution nonsense
zu405 Allele substitution
zu196 Transposon insertion insertion