Laboratory Information
| Name | JJ View on WormBase |
|---|---|
| Allele designation | zu |
| Head | James R Priess |
| Institution | Fred Hutchinson Cancer Research Center, Seattle, WA |
| Address | Fred Hutchinson Cancer Center Room A3-123 Weintraub Building 1100 Fairview Ave North Seattle 98109 United States |
| Website | http://labs.fhcrc.org/priess/jim.html |
| Gene classes | aph apx arg bop eel hmp hmr mex mom pie pop skn |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| EU1 | skn-1(zu67) IV/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Maintain by picking Unc. |
| JJ1014 | mex-3(zu155) dpy-5(e61)/hT1 I; pos-1(zu148) unc-42(e270)/hT1 V. | C. elegans | The double heterozygote is WT. WT will segregate WT, DpyUncs, mid-larval lethal (hT1 homozygotes) and dead eggs. The DpyUncs are homozygous for zu155 and zu148 and will segregate only dead embryos; the dead embryos have excess hypodermis and muscle and lack germ cells and intestine. |
| JJ1057 | pop-1(zu189) dpy-5(e61)/hT1 I; him-5(e1490)/hT1 V. | C. elegans | Heterozygotes are WT and segregate WT, Dpys which give only dead eggs, mid-larval lethals (hT1 homozygotes), males and dead eggs. Mutation in pop-1 results in the MS blastomere adopting the fate of the E blastomere. pop-1 mutant embryos have twice the amount of wild type gut and only make anterior pharynx. him-5 is outside the region balanced by hT2. |
| JJ1079 | hmr-1(zu389)/lin-11(n566) unc-75(e950) I. | C. elegans | Heterozygotes are WT and segregate WT, Hmr inviable embyros and Egl Unc. Hmr: Hammerhead - defective hypodermal enclosure, especially in anterior regions; approximately 2% of zu389 embryos enclose normally and are Hmp [Humpback: defective body elongation, abnormal bulges on dorsal side]. See also WBPaper00005031. Received new stock from Allison Lynch in the Hardin lab 3/2009. |
| JJ1129 | elt-1(zu180) unc-43(e408)/unc-24(e138) dpy-20(e1282) IV. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and dead eggs. |
| JJ1142 | hmr-1(zu248) I; zuEx2. | C. elegans | zuEx2 [W02B9(cosmid) + rol-6(su1006). Maintain by picking Rollers. |
| JJ1237 | mex-6(pk440) II. | C. elegans | Viable, fertile, apparently normal. |
| JJ1238 | unc-30(e191) mex-5(zu199) IV/nT1 (IV;V). | C. elegans | Heterozygotes are WT. Embryos from unc-30 mex-5 homozygotes produce approximately the WT number of cells but do not undergo body morphogenesis and die without hatching. |
| JJ1244 | mex-6(pk440) II; unc-30(e191) mex-5(zu199) IV/nT1 (IV;V). | C. elegans | Heterozygotes are WT. Embryos from mex-6; unc-30 mex-5 homozygotes produce approximately the WT number of cells but do not undergo body morphogenesis and die without hatching. |
| JJ1271 | glo-1(zu391) X. | C. elegans | Lacks autofluorescent and birefringent gut granules. |
| JJ1440 | unc-119(ed3) III; zuIs20. | C. elegans | zuIs20 [par-3p::par-3::ZF1::GFP + unc-119(+)]. GFP-tagged PAR-3 that is degraded in early embryonic somatic cells. Worms carrying the transgene are non-Unc and express GFP very weakly in early embryos (only detectable by antibody staining), and later in adherens junctions of epithelial cells. |
| JJ1473 | unc-119(ed3) III; zuIs45 V. | C. elegans | zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. NMY-2::GFP is expressed in the germline and somatic gonad. |
| JJ1494 | unc-119(ed3) III; zuIs58. | C. elegans | zuIs58 [unc-119(+) + par-6::PAR-6::ZF1::GFP]. |
| JJ1508 | unc-119(ed3) III; zuIs60. | C. elegans | zuIs60 [pie-1p::GFP(secreted) + unc-119(+)]. Maternally-expressed secreted GFP fills spaces between embryonic cells, and space between embryo and vitelline membrane. Useful marker for vizualizing intercellular spaces in embryos. Reference: Wehman AM, et al. Curr Biol. 2011 Dec 6;21(23):1951-9. |
| JJ1549 | efl-1(se1) V. | C. elegans | Temperature sensitive. At restrictive temperature (26C), efl-1 produces dead eggs with a Mex phenotype. Two ts periods: 1) prior to L4 results in sterility, 2) after L4 results in a maternal-effect embryonic lethal phenotype. |
| JJ1550 | dpl-1(zu355) unc-4(e120)/rol-6(e187) let-23(sy97) II. | C. elegans | Heterozygotes are WT and segregate WT, Uncs which give only deads egss with a Mex phenotype, and Vulvaless Rollers. sy97 is only 15% viable. |
| JJ1833 | dpy-5(e61) I; unc-32(e189) III. | C. elegans | Dpy Unc. |
| JJ1850 | unc-119(ed3) III; zuIs178. | C. elegans | zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. HIS-72::GFP can be detected in germline and most somatic nuclei, but not in intestinal nuclei. Not integrated on X. |
| JJ1851 | unc-119(ed3) III; zuEx181. | C. elegans | zuEx181[his-72(1kb 5' UTR)::YFP::GSRPVAT::HIS-72::HIS-72 (1KB 3'UTR) + 5.7 kb XbaI - HindIII UNC-119(+)]. HIS-72::YFP signal can be detected in the germline and most somatic nuclei, but not in intestinal nuclei. |
| JJ1852 | unc-119(ed3) III; zuEx182. | C. elegans | zuEx182[his-71(1kb 5' UTR):: HIS-71::GSRPVAT::GFP::::HIS-71 (1KB 3'UTR) + 5.7 kb XbaI - HindIII UNC-119(+)]. HIS-71::YFP signal can be detected in most somatic nuclei, including the intestinal nuclei, but is not detected in the germline. |
| JJ1972 | eel-1(zu462) unc-33(e204) IV. | C. elegans | Slow growth and a maternal-effect enhancer of the efl-1(se1) embyronic lethal phenotype. Unc. |
| JJ1992 | unc-119(ed3) III; zuIs145. | C. elegans | zuIs145 [nmy-2p::mom::GFP + unc-119(+)]. Wild type phenotype. |
| JJ2059 | unc-119(ed3) III; zuIs235. | C. elegans | zuIs235 [his-72(1-kb 5' UTR):: BIOTAG::3XHA::HIS-72::his-72(1-kb 3' UTR) + unc-119(+)]. Superficially wild-type. |
| JJ2060 | unc-119(ed3) III; zuIs236. | C. elegans | zuIs236 [his-72(1-kb 5' UTR)::BIRA::GFP:: his-72(1-kb 3' UTR) + unc-119(+)]. Superficially wild-type. |
| JJ2061 | unc-119(ed3) III; zuIs235; zuIs236. | C. elegans | zuIs235 [his-72(1-kb 5' UTR):: BIOTAG::3XHA::HIS-72::his-72(1-kb 3' UTR) + unc-119(+)]. zuIs236 [his-72(1-kb 5' UTR)::BIRA::GFP:: his-72(1-kb 3' UTR) + unc-119(+)]. Superficially wild-type. |
| JJ2281 | unc-119(ed3) III; zuIs258. | C. elegans | zuIs258 [his-72p::his-72(5' UTR)::BIRA::GFP::his-72(3' UTR) + unc-119(+)]. GFP expression detectable in embryos. Reference: Steiner FA, et al. Genome Res. 2012 Apr;22(4):766-77. |
| JJ2286 | unc-119(ed3) III; zuIs263. | C. elegans | zuIs263 [myo-3p::myo-3(5' UTR)::npp-9::mCherry::BLRP::3xFLAG::npp-9(3' UTR) + unc-119(+)]. Reference: Steiner FA, et al. Genome Res. 2012 Apr;22(4):766-77. |
| JJ2300 | unc-119(ed3) III; zuIs258; zuIs263. | C. elegans | zuIs258 [his-72p::his-72(5' UTR)::BIRA::GFP::his-72(3' UTR) + unc-119(+)]. GFP expression detectable in embryos. zuIs263 [myo-3p::myo-3(5' UTR)::npp-9::mCherry::BLRP::3xFLAG::npp-9(3' UTR) + unc-119(+)]. Reference: Steiner FA, et al. Genome Res. 2012 Apr;22(4):766-77. |
| JJ2586 | cox-4(zu476[cox-4::eGFP::3xFLAG]) I. | C. elegans | cox-4(zu476[cox-4::eGFP::3xFLAG]) I. Endogenous cox-4 locus tagged with eGFP via genome editing. Mitochondria in all cell types are labeled with GFP. Reference: Raiders SA, et al. PLoS Genet. 2018 Jul 19;14(7):e1007417. |
| JJ462 | +/nT1 IV; pos-1(zu148) unc-42(e270)/nT1 V. | C. elegans | Heterozygotes are WT and segregate WT, Uncs, Vul and dead eggs. Uncs are homozygous for zu148 and will segregate only dead embryos; the dead embryos will have no morphogenesis and will lack germ cells and intestine. |
| JJ478 | mex-3(zu155) egl-30(n686)/hT1 I; +/hT1 V. | C. elegans | Heterozygotes are WT and segregate WT, Egls which give only dead eggs, dead eggs, and mid-larval lethals (hT1 homozygotes) |
| JJ529 | rol-1(e91) mex-1(zu121)/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | Heterozygotes are WT and segregate WT, DpyUnc and Rollers. Rollers give only dead eggs. Maintain by picking WT. mex-1 is to the right of rol-1, is uncovered by mnDf87 and is allelic to mel-21. |
| JJ532 | pie-1(zu154) unc-25(e156)/qC1 [dpy-19(e1259) glp-1(q339)] III. | C. elegans | Heterozygotes are WT and segregate WT, Unc and DpySteriles. Uncs give only dead eggs. Maintain by picking WT. |
| JJ746 | +/nT1 IV; apx-1(zu183) dpy-11(e224)/nT1 V. | C. elegans | Heterozygotes are WT and segregate WT, Dpy, Vul and dead eggs. The Dpys give only dead eggs. apx-1 is a maternal effect lethal. zu183 is recessive. |
| JJ862 | hmp-1(zu278)/daf-11(m84) sma-1(e30) V. | C. elegans | Heterozygotes are WT and segregate WT, Hmp inviable embyros (Hmp: humpback-defective body elongation, abnormal bulges on dorsal side) and Daf Sma (ts Daf). |
| JJ938 | unc-24(e138) daf-14(m77)/elt-1(zu180) dpy-20(e1282) IV. | C. elegans | At 20C or 25C, heterozygotes are WT and segregate WT, UncDaf and dead eggs. At 15C, heterozygotes are WT and segregate WT, Uncs and dead eggs. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| zu181 | Transposon insertion | insertion | |
| zu67 | Allele | substitution | nonsense |
| zu154 | Allele | ||
| zu135 | Allele | substitution | nonsense |
| zu169 | Allele | substitution | nonsense |
| zu129 | Allele | substitution | nonsense |
| zu193 | Allele | ||
| zu170 | Transposon insertion | insertion | |
| zu446 | Allele | substitution | nonsense |
| zu456 | Allele | substitution | nonsense |
| zu310 | Allele | substitution | |
| zu155 | Allele | ||
| zu148 | Allele | substitution | nonsense |
| zu189 | Allele | ||
| zu364 | Allele | substitution | |
| zu389 | Allele | substitution | nonsense |
| zu180 | Allele | substitution | |
| zu248 | Allele | ||
| zu199 | Allele | ||
| zu391 | Allele | substitution | splice_site |
| zu355 | Allele | ||
| zu462 | Allele | insertion | |
| zu121 | Allele | ||
| zu183 | Allele | ||
| zu278 | Allele | substitution | nonsense |
| zu222 | Transposon insertion | insertion | |
| zu225 | Allele | substitution | nonsense |
| zu405 | Allele | substitution | |
| zu196 | Transposon insertion | insertion |