Laboratory Information
| Name | JIM View on WormBase |
|---|---|
| Allele designation | uj |
| Head | John Isaac Murray |
| Institution | University of Pennsylvania, Philadelphia, PA |
| Address | 440 Clinical Research Building 415 Curie Boulevard Philadelphia 19104-6145 United States |
| Website | http://www.med.upenn.edu/murraylab/ |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| JIM113 | ujIs113 II. | C. elegans | ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)]. Reference: Zacharias A, et al. PLoS Genet. 2015 Oct 21;11(10):e1005585. |
| JIM173 | unc-119(tm4063) III; ujEx173. | C. elegans | ujEx173 [ceh-36::TY1::EGFP::3xFLAG + unc-119(+)]. Pick non-Unc to maintain. Reference: Walton T, et al. PLoS Genet. 2015 Mar 4;11(3):e1005003. |
| JIM193 | ujIs113 II; ujIs193. | C. elegans | ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)] II. ujIs193 [nhr-67::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence in fosmid ID#WRM0633cC01 by recombineering. Expression of transgene confirmed by GFP. |
| JIM220 | ujIs113 II; unc-30(ok613) IV; ceh-36(ok795) X; ujEx173. | C. elegans | ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)] II. ujEx173 [ceh-36::TY1::eGFP::3xFLAG + unc-119(+)]. ujEx173 rescues unc-36, suppressing synthetic lethality in animals carrying the array. Reference: Walton T, et al. PLoS Genet. 2015 Mar 4;11(3):e1005003. |
| JIM271 | ujIs113 II; stIs10286. | C. elegans | ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)]. stIs10286 [nob-1::GFP::unc-54 3'UTR + rol-6(su1006)]. stIs10286 contains 9kb promoter and full nob-1 transcript fused to GFP. Reference: Zhao Z, et al. PLoS Genet. 2010 Sep 2;6(9):e1001089. |
| JIM356 | ujIs113 II; ujIs153. | C. elegans | ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)] II. ujIs153 [ceh-13::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence in fosmid ID#WRM0622c06 by recombineering. Expression of transgene confirmed by GFP. |
| PHX2605 | ceh-13(syb2605[ceh-13::GFP]) III. | C. elegans | GFP tag inserted at the C-terminus of the endogenous ceh-13 locus by CRISPR. Reference: Murray JI, et al. PLOS Genet. 2022 May 2;18(5):e1010187. PMID: 35500030 |
| PHX2679 | nob-1(syb2679[nob-1::GFP]) III. | C. elegans | GFP tag inserted at the C-terminus of the endogenous nob-1 locus by CRISPR. Reference: Murray JI, et al. PLOS Genet. 2022 May 2;18(5):e1010187. PMID: 35500030 |
| PHX5791 | pop-1(syb5791[GFP::AID::GGGGSGSGS linker::pop-1]) I. | C. elegans | GFP and AID tags inserted at the N-terminus of the endogenous pop-1 locus by CRISPR. Insertion includes a GGGGSGSGS linker sequence between the tags and POP-1. Generated in N2 background. |
| RW20022 | Cbr-unc-119(stIs20000) III; stIs20022. | C. briggsae | stIs20022 [Cbr-his-72::GFP::pie-1 3'UTR + Cbr-unc-119(+)]. C. briggsae with fluorescently labeled (GFP) histones. Reference: Zhou Z, et al. Genetics. 2010 Mar; 184(3): 853–863. doi: 10.1534/genetics.109.110270 PMID: 20008572. |
| RW20025 | Cbr-unc-119(stIs20000) III; stIs20025. | C. briggsae | stIs20025 [Cbr-his-72::mCherry::pie-1 3'UTR + Cbr-unc-119(+)]. C. briggsae with fluorescently labeled (mCherry) histones. Reference: Zhou Z, et al. Genetics. 2010 Mar; 184(3): 853–863. doi: 10.1534/genetics.109.110270 PMID: 20008572. |
| WU970 | haly-1(am132) X. | C. elegans | Resistance to excess dietary zinc and nickel observed on noble agar minimal media with supplemental zinc or nickel; elevated levels of histidine observed in C. elegans on minimal media. Reference: Murphy JT, et al. PLoS Genet. 2011 Mar;7(3):e1002013. |
| WU971 | haly-1(am130) X. | C. elegans | Resistance to excess dietary zinc and nickel observed on noble agar minimal media with supplemental zinc or nickel; elevated levels of histidine observed in C. elegans on minimal media. Reference: Murphy JT, et al. PLoS Genet. 2011 Mar;7(3):e1002013. |
This laboratory hasn't submitted any alleles to the CGC.