Laboratory Information

NameIX View on WormBase
Allele designationvy
HeadChuang, Chiou-Fen
InstitutionUniversity of Illinois at Chicago, Chicago, IL
Address 900 S. Ashland Ave
MBRB 4068, MC567
University of Illinois at Chicago
Chicago 60607
United States
Website https://sites.google.com/site/chioufenchuanglab/
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
IX3447 kyIs140 I; vyEx1462. C. elegans kyIs140 [str-2::GFP + lin-15(+)] I. vyEx1462 (die-1(fosmid)::Venus + odr-1::DsRed + ofm-1::DsRed). Pick animals with DsRed+ coelomocytes to maintain. Animals with vyEx1462 show 2AWC(OFF) phenotype. Extrachromosomal array rescues die-1(w34).
IX3597 kyIs140 I; vyEx1510. C. elegans kyIs140 [str-2::GFP + lin-15(+)] I. vyEx1510 (ceh-36p::die-1(cDNA) + odr-1::DsRed + ofm-1::DsRed). Pick animals with DsRed+ coelomocytes to maintain. Animals with vyEx1510 show 2AWC(OFF) phenotype.
IX4506 mls-2(vy248[mNG::mls-2]) X. C elegans mNG tag inserted into endogenous mls-2 locus after the start codon using CRISPR/Cas9 engineering, producing a translational mNG::MLS-2 reporter protein. Derived by SEC excision of mls-2(vy247[mNG::SEC::mls-2 knock-in]) in parental strain. mNG::MLS-2 is detected in the nucleus of a few cells in embryos, and localizes to the nucleus of a subset of head cells and the M mesoblast in larvae and adults. Reference: Xiong R., et al. (2022). mNG-tagged mls-2 knock-in alleles in C. elegans. microPublication Biology. 10.17912/micropub.biology.000529.
IX4507 mls-2(vy247[mNG::SEC::mls-2]) X. C elegans The mNG::SEC::mls-2 knock-in allele, a transcriptional reporter of mls-2, contains transcriptional terminators in the self-excising cassette (SEC). Diffuse mNG expression of mNG::SEC::mls-2 knock-in is visible in a subset of cells from bean-stage embryos throughout the embryogenesis stages. It also shows diffuse mNG expression in numerous cells in the head and the M mesoblast of larval and adult stages.
This laboratory hasn't submitted any alleles to the CGC.