Laboratory Information

NameIC View on WormBase
Allele designationqu
HeadIan D Chin-Sang
InstitutionQueen's University, Kingston, ON, Canada
Address Queen's University
Department of Biology
116 Barrie St.
Kingston K7L 3N6
Canada
Website http://www.chin-sang.ca
Gene classes afd  nck 

Strains contributed by this laboratory

Strain Genotype Species Description
CZ4111 vab-2(ju1) IV. C. elegans vab-2(ju1) has embryonic lethality (12%) and notched heads (about 40%). vab-2(ju1) is considered a null allele (W30opal). pka efn-1.
IC136 zdIs5 I; vab-1(dx31) II. C. elegans zdIs5 [mec-4::GFP + lin-15(+)].Animals contain GFP expressed in the touch neurons and have a notched head phenotype.
IC156 zdIs5 I; quIs4. C. elegans zdIs5 [mec-4::GFP + lin-15(+)]. quIs4 [mec-4::myr::vab-1(G912E) + rol-6(su1006)]. Animals contain GFP expressed in the touch neurons.
IC361 vab-1(e2)/mIn1 [dpy-10(e128) mIs14] II; sax-3(ky123) X. C. elegans Heterozygotes are WT and GFP+. mIn1 homozygotes are Dpy and GFP+. vab-1; sax-3 homozygotes are synthetic lethal.
IC400 zdIs5 I; quIs5 II; him-5(e1490) V. C. elegans zdIs5 [mec-4::GFP + lin-15(+)]. quIs5 [mec-4::myr::vab-1 + rol-6(su1006)]. Animals contain GFP expressed in the touch neurons.
IC450 sax-3(ky123) X; quEx89. C. elegans quEx89 [sax-3::GFP + rol-6(su1006)]. sax-3::GFP partially rescues the lethality and notch phenotype of sax-3(ky123). Maintain by picking Rollers.
IC459 sax-3(ky123) X; quEx102. C. elegans quEx102[F25B3.3::SAX-3 + odr-1::RFP]. F25B3.3::SAX-3 partially rescues the lethality and notch phenotype of sax-3(ky123). Maintain by picking RFP+.
IC464 sax-3(ky123) X; quEx100. C. elegans quEx100 [ajm-1::sax-3 + odr-1::RFP]. ajm-1::sax-3 partially rescues the lethality of sax-3(ky123). Maintain by picking RFP+.
IC476 sax-3(ky123) X; quEx99. C. elegans quEx99 [sax-3(minigene) + odr-1::RFP]. Rescues the lethality of ky123. Pick RFP+ to maintain.
IC611 quEx128. C. elegans quEx128 [npr-9::GFP + rol-6(su1006)]. Maintain by picking Rollers. ZK455.3 is npr-9 and is expressed in AIB neuron.
IC683 npr-9(tm1652) X. C. elegans
IC692 quEx162. C. elegans quEx162 [sax-3p::GFP + rol-6(su1006)]. Pick GFP to maintain. Very bright GFP in early embryos (after 24 cell stage). Not all GFP+ worms are rolling.
IC699 sax-3(ky123) ; quEx168. C. elegans quEx168 [sax-3::GFP + odr-1::RFP]. Pick RFP+ to maintain. GFP is visible at higher magnification but fades quickly. Strongest GFP is in the head region. The Chin-Sang Lab recommends researchers use this strain instead of IC450, as sax-3::GFP expression in IC699 is more consistent than in the comparable strain IC450.
IC700 juIs73 II; vab-2(ju1) IV. C. elegans juIs73 [unc-25p::GFP] II.
IC765 npr-9(tm1652) X; quEx182. C. elegans quEx182 [npr-9(+) + sur-5::GFP + odr-1::RFP]. Maintain by picking GFP+. Rescuing npr genomic fragment co-injected with sur-5::GFP and odr-1::RFP]. transgenic (GFP+) animals tend to wander off the food.
temp_name295 sax-3(ky123) X; quEx89. IC Ecotpic and inconsistent sax-3::GFP expression
This laboratory hasn't submitted any alleles to the CGC.