Laboratory Information

NameHZ View on WormBase
Allele designationbp
HeadHong Zhang
InstitutionInstitute of Biophysics, Chinese Academy of Sciences, Beijing, China
Address Datun Road NO.15,Chaoyang District,Beijing,China
Beijing 100101
China
Gene classes epg  nfya  nfyb  nfyc  sepa  sor  sqst 

Strains contributed by this laboratory

Strain Genotype Species Description
HZ107 him-5(e1490) bxIs13 V; nfya-1(bp4) X. C. elegans bxIs13 [egl-5::GFP + lin-15(+)]. Him. Unc. Ectopic expression of GFP in head neurons.
HZ111 muIs16 II; sor-1(bp_1)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-5(e1490) V. C. elegans muIs16 [mab-5::GFP]. Larval lethal at L3/L4 stage. Ectopic expression of GFP in the arrested sor-1 mutant homozygotes (L3 stage). NOTE: This allele is published as bp1, but has been curated in WormBase as bp_1 in order to differentiate it from the STS marker bP1.
HZ112 muIs16 II; sor-1(bp3)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-5(e1490) V. C. elegans muIs16 [mab-5::GFP]. Larval lethal at L3/L4 stage. Ectopic expression of GFP in the arrested sor-1 mutant homozygotes (L3 stage).
HZ113 him-5(e1490) bxIs13 V; nfya-1(bp5) X. C. elegans bxIs13 [egl-5::GFP + lin-15(+)]. Him. Unc. Ectopic expression of GFP in head neurons.
HZ1569 bpIs239. C. elegans bpIs239 [W07G4.5p::W07G4.5::GFP + unc-76(+)]. W07G4.5::GFP is expressed in the intestine. A few GFP aggregates are formed in wild-type embryos at the four-fold stage; the number of aggregates is dramatically increased in epg-7 and atg-3 mutants. Reference: Lin L, et al. J Cell Biol. 2013 Apr 1;201(1):113-29.
HZ1683 him-5(e1490) V; atg-2(bp576) X. C. elegans Him; ~30% male.
HZ1684 atg-3(bp412) IV; him-5(e1490) V. C. elegans Him; ~30% male.
HZ1685 atg-4.1(bp501) I. C. elegans
HZ1686 bnIs1 I; atg-7(bp411) IV; him-5(e1490) V. C. elegans bnIs1 [pie-1p::GFP::pgl-1 + unc-119(+)] I. Him; ~30% male. Accumulation of GFP::PGL-1 aggregates in somatic cells in embryos.
HZ1687 atg-9(bp564) him-5(e1490) V. C. elegans Him; ~30% male.
HZ1688 atg-13(bp414) III. C. elegans
HZ1690 epg-6(bp242) III; him-5(e1490) V. C. elegans Him; ~30% male.
HZ1691 epg-8(bp251) I; him-5(e1490) V. C. elegans Him; ~30% male.
HZ1692 epg-9(bp320) IV. C. elegans Him; ~30% male.
HZ194 apr-1(bp298) I; wIs51 V. C. elegans wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. apr-1(bp298) is a G338E missense mutation disrupts the normal asymmetric division pattern of seam cells, causing symmetric divisions. Reference: Huang XX, et al. Dev Biol. 2009 Sep 15;333(2):337-47.
HZ203 bro-1(bp133) I; him-5(e1490) wIs51 V. C. elegans wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. bro-1 adults have an average of 11 seam cells. Reference: Xia D, et al. Dev Biol. 2007 Sep 15;309(2):259-72.
HZ455 him-5(e1490) V; bpIs131. C. elegans bpIs131 [sepa-1p::sepa-1::GFP + unc-76(+)]. Him. SEPA-1::GFP aggregates form in a temporal pattern. Diffuse SEPA-1::GFP is detectible in most cells at the comma stage and greatly diminished by the 2-fold stage. After hatching, cytoplasmic SEPA-1::GFP aggregates were found in a few unidentified cells in the head and tail regions and also in the intestine, especially in the anterior and posterior pairs of intestine cells. Reference: Tian Y, et al. Cell. 2010 Jun 11;141(6):1042-55.
HZ470 sea-2(bp283) II; wIs51 V. C. elegans wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells.
HZ504 him-5(e1490) V; bpIs37. C. elegans bpIs37 [dcap-1p::dcap-1::DsRed + rol-6(su1006)]. Rollers. Translational DsRed reporter for the P body-specific marker dcap-1, which encodes the C. elegans ortholog of decapping complex component DCAP1. Low levels of expression are homogenously distributed in the cytoplasm at all stages of embryogenesis. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39.
HZ589 him-5(e1490) V; bpIs151. C. elegans bpIs151 [sqst-1p::sqst-1::GFP + unc-76(+)]. Him. In wild-type embryos, sqst-1::GFP is very weakly expressed and diffusely localized in the cytoplasm. Reference: Tian Y, et al. Cell. 2010 Jun 11;141(6):1042-55.
HZ620 ceh-16(bp323) III; wIs51 V. C. elegans wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Temperature-sensitive: maintain at 20C or lower. bp323 mutants show reduced number of seam cells when raised at restrictive temperature (25C). Reference: Huang XX, et al. Dev Biol. 2009 Sep 15;333(2):337-47.
HZ66 sop-2(bp186) II; him-5(e1490) bxIs16 V. C. elegans bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C.
HZ67 him-5(e1490) bxIs16 V; sor-3(bp185) X. C. elegans bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C.
HZ769 him-5(e1490) V; bpIs88. C. elegans bpIs88 [tia-1p::tia-1::GFP + dcap-1p::dcap-1::RFP + rol-6(su1006)]. Rollers. Rolling phenotype is more apparent when raised >20C. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39.
HZ771 him-5(e1490) V; bpIs90. C. elegans bpIs90 [tia-1p::tia-1::GFP + rol-6(su1006)]. Rollers. Rolling phenotype is more apparent when raised >20C. TIA-1::GFP is homogenously distributed in the cytoplasm during embryogenesis. At larval stages, diffuse TIA-1::GFP signals are observed in seam cells and hypodermal cells. TIA-1::GFP forms distinct bodies, especially in seam cells and hypodermal cells, under stress conditions. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39.
HZ946 rpl-43(bp399) II; bpIs151. C. elegans bpIs151 [sqst-1p::sqst-1::GFP + unc-76(+)]. bp399 mutants accumulate SQST-1 aggregates strictly in the intestine in a distinct temporal pattern. SQST-1::GFP aggregates are absent in bp399 embryos, but start to form in L1 larvae and increase in number and size throughout larval development. Reference: Guo B, et al. EMBO Rep. 2014 Jun;15(6):705-13.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
bp4 Allele substitution nonsense
bp_1 Allele substitution
bp3 Allele substitution nonsense
bp5 Allele substitution
bp576 Allele substitution nonsense
bp412 Allele
bp501 Allele substitution nonsense
bp564 Allele substitution nonsense
bp414 Allele substitution splice_site
bp242 Allele substitution nonsense
bp251 Allele substitution nonsense
bp320 Allele substitution nonsense
bp298 Allele
bp283 Allele substitution
bp323 Allele
bp186 Allele substitution
bp185 Allele substitution nonsense
bp399 Allele substitution