Laboratory Information
Name | HR View on WormBase |
---|---|
Allele designation | sb |
Head | Mains, Paul |
Institution | University of Calgary, Calgary, Alberta |
Address | Biochemistry and Molecular Biology University of Calgary 3330 Hospital Dr. NW Calgary T2N4N1 Canada |
Website | http://www.ucalgary.ca/bmb/MAINS |
Gene classes | fhod ing ppfr |
Strains contributed by this laboratory
Strain | Genotype | Species | Description |
---|---|---|---|
BW1102 | dpy-5(e61) mei-2(ct102) unc-29(e1072) I; sDp2 (I;f). | C. elegans | Animals with the duplication are Unc. Animals that have lost the duplication are DpyUncMel. mei-2 is non-conditional recessive maternal effect lethal. |
BW1118 | mel-25(ct60) unc-42(e270)/unc-23(e25) vab-8(e1017) V; lon-2(e678) X. | C. elegans | Maintain by picking Lon non-Unc. Throws Lon non-Unc, Lon Unc Vab (bent head, tails very thin), and Lon Unc Mel (kinker, temperature-sterile). |
HR10 | dhc-1(ct42) dpy-5(e61)/unc-11(e47) bli-4(e937) I. | C. elegans | Heterozygtes are WT. Hets segregate WT, UncBli and DpyLet. ct42 homozygotes are inviable at all temperatures (recessive non-conditional larval lethal). ct42 is dominant temperature sensitive maternal effect lethal. ct42 hets give more inviable progeny at 25C than at 15C. dch-1 was previously called let-354(ct42). |
HR1060 | tbb-2(sb26) III. | C. elegans | Behaves as wild-type. |
HR1184 | +/szT1 [lon-2(e678)] I; nmy-1(sb115) dpy-8(e130)/szT1 X. | C. elegans | Heterozygotes are WT and segregate WT, Dpy Uncish with occasional Rol (nmy-1 dpy-8), Lon males, and dead eggs. nmy-1 homozygotes have a low brood size and are slow growing. sb115 is a null, truncation allele. |
HR1275 | src-1(cj293) dpy-5(e61)/hT2 [bli-4(e937) let-?(h661)] I; +/hT2 III. | C. elegans | Heterozygotes are WT and segregate WT, Dpy Mels and dead eggs. |
HR1327 | mel-26(tm1664) unc-29(e1072) I. | C. elegans | Temperature sensitive. Maintain at 15C. Unc. |
HR16 | mel-23(ct45)/qC1 [dpy-19(e1259) glp-1(q339)] III. | C. elegans | Heterozygotes are WT and segregate WT and DpySteriles. ct45 homozygotes give only dead eggs at all temperatures. ct45 is ts dominant. Hets give more viable progeny at 15C than 25C. |
HR483 | mel-11(sb56) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Unc Steriles that are slightly Long. sb56 is a recessive zygotic suppressor of let-502(ca201) early larval lethality. Likely null of mel-11. |
HR492 | +/hT2 I; vab-7(e1562) mel-44(sb44)/hT2 [bli-4(e937) let-?(h661)] III. | C. elegans | Dominant ts maternal-effect embryonic lethal. Embryos arrest prior to morphogenesis or at two-fold. Non-ts recessive mid-larval lethal. Maintain at 15C. Freezes poorly. |
HR505 | unc-59(e261) tba-2(sb51)/hIn1 [unc-54(h1040)] I. | C. elegans | Dominant temperature sensitive maternal-effect embryonic lethal. Maintain at 15C. Heterozygotes are WT. hIn1 homozygotes are Unc. Early cleavage spindles small and misoriented, cytokinesis often incomplete. Recessive non-temperature sensitive maternal-effect embryonic lethal (but sterile in conjuction with unc-59). |
HR592 | memi-1(sb41) dpy-20(e1282)/nT1 [unc-?(n754) let-?] IV; +/nT1 V. | C. elegans | Dominant ts maternal-effect embryonic lethal. Embryos show extensive cytoplasmic blebbing, often accompanied by small spindles and incomplete cytokinesis. Two cell embryos divide synchronously. Embryos arrest with eight to several hundred cells. Recessive non-ts maternal-effect embryonic lethal. Null may be WT. Maintain at 15C. |
HR596 | sbEx133. | C. elegans | sbEx133 [mel-11::GFP + rol-6(su1006)]. Maintain by picking Rollers. |
HR606 | sbEx136. | C. elegans | sbEx136 [(pAW33.1) let-502p::GFP + rol-6(su1006)]. pAW33.1 is about 6.6 kb BamH1 fragment from K06G1 and includes the first 12 amino acids of LET-502 clones into pPD95.67/BamH1. Should be nuclear localized, but is not nuclear localized. Maintain by picking Rollers. |
HR75 | mei-1(b284) unc-29(e1072)/mei-1(ct46) unc-13(e1091) I. | C. elegans | Heterozygotes are WT. ct46 is a dominant, temperature sensitive maternal effect lethal. ct46 homozygotes are viable and fertile at 15C. b284 is a non-conditional, recessive maternal effect lethal. Heterozygotes give more viable progeny at 15C than 25C. |
HR767 | daf-8(e1393) mel-26(ct61sb4) I. | C. elegans | ct61 is a dominant, temperature sensitive, maternal effect lethal. sb4 is a revertant of the dominant phenotype. sb4 is a recessive, temperature sensitive, maternal effect lethal with approx. 10% hatching at 15C and 0% at 25C. |
HR83 | mel-24(ct59) dpy-20(e1282)/unc-24(e138) daf-15(m81) IV. | C. elegans | Heterozygotes are WT and segregate WT, Unc Dauers, and Dpys which throw dead eggs. Maintain by picking WT. ct59 animals are viable and fertile at 15C. ct59 is a temperature sensitive dominant maternal effect lethal. ct59 hets give more viable offspring at 15C than 25C. |
HR890 | +/szT1 [lon-2(e678)] I; syDf1/szT1 X. | C. elegans | Heterozygotes are Lon and segregate Lon and dead eggs. |
HR899 | unc-101(m1) tba-2(sb25) I. | C. elegans | Unc. |
HR961 | ced-7(n1829) dpy-17(e164) III. | C. elegans | Dpy. Maternal effect persistent cell corpses. This was derived from MT4993; it was outcrossed once to get rid of an unlinked lethal. Hatching rate has improved from 10-70% to 98%. |
HR973 | unc-101(m1) tba-2(sb27) I. | C. elegans | Unc. |
HR98 | mel-26(ct61) unc-29(e1072) I. | C. elegans | Dominant temperature sensitive maternal effect lethal. Will segregate some Vab. Maintain at 15C: about 30% of homozygotes hatch at 15C. Will not grow at 20C or 25C. Unc. |
Alleles contributed by this laboratory
Allele | Type | DNA Change | Protein Change |
---|---|---|---|
sb26 | Allele | substitution | |
sb115 | Allele | substitution | nonsense |
sb56 | Allele | ||
sb44 | Allele | ||
sb51 | Allele | substitution | |
sb41 | Allele | ||
sb4 | Allele | ||
sb25 | Allele | substitution | |
sb27 | Allele | substitution | |
sb118 | Allele |