Laboratory Information
| Name | HC View on WormBase |
|---|---|
| Allele designation | qt |
| Head | Craig P Hunter |
| Institution | Harvard University, Cambridge, MA |
| Address | 16 Divinity Ave. BL3050 Cambridge 02138 United States |
| Website | http://mcb.harvard.edu/hunter/ |
| Gene classes | scrt sid |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| HC114 | ccIs4251 I; qtIs3 III; mIs11 IV; sid-1(qt9) V. | C. elegans | ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. qtIs3 [myo-2p::GFP dsRNA hairpin]. mIs11 [myo-2p::GFP + pes-10p::GFP + gut-promoter::GFP]. GFP expression in 4-cell embryos, pharyngeal muscle and gut. Resistant to systemic RNAi by feeding and injection and endogenous hairpin expression. |
| HC1292 | sid-1(qt160) V. | C. elegans | sid-1(qt160) V. Null allele. Systemic RNAi deficient. This sid-1(qt160) allele is designed as a efficient Cas9 target for reversion to wild-type sid-1 function in co-conversion experiments. Successful reversion re-enables RNAi targeting any gene of choice. 44 nt sid-1 loss of function cassette inserted into exon 2 (Chr V: 5120123..5120124) contains exogenous crRNA site UcrRNA_AW1, stop codons in all three frames, and KpnI site, which also induces a frame shift. sid-1 LOF cassette sequence: ccgccgcactggacaaacttccctaactgactaaggtaccgata. Derived by 6x out-crossing of parental strain HC1185. Reference: Weisman, Fisher, and Hunter 2025. G3. In press. |
| HC196 | sid-1(qt9) V. | C. elegans | Null allele. Systemic RNAi defective. Reference: Jose AM, et al. Proc Natl Acad Sci U S A. 106(7):2283-8. |
| HC271 | ccIs4251 I; qtIs3 sid-2(qt42) III; mIs11 IV. | C. elegans | ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. qtIs3 [myo-2p::GFP dsRNA hairpin]. mIs11 [myo-2p::GFP + pes-10p::GFP + gut-promoter::GFP]. GFP expression in 4-cell embryos, pharyngeal muscle and gut. Resistant to systemic RNAi by feeding only. |
| HC48 | tbb-2(qt1) III. | C. elegans | Temperature sensitive embryonic lethal mutation with defects in centration and rotation of the centrosome pronuclear complex in the first cell division. Maintain at 15C. |
| HC726 | sid-1(qt95) V. | C. elegans | Sid. Hypomorhic allele. Reference: Whangbo JS, et al. G3 (Bethesda). 2017 Oct 12 [Epub ahead of print]. pii: g3.300308.2017. doi: 10.1534/g3.117.300308. PMID: 29025917. |
| HC75 | ccIs4251 I; sid-1(qt2) V. | C. elegans | ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. Resistant to systemic RNAi, but sensitive to autonomous RNAi. |
| HC970 | sid-1(qt78) V. | C. elegans | Sid. Deletion allele. Reference: Whangbo JS, et al. G3 (Bethesda). 2017 Oct 12 [Epub ahead of print]. pii: g3.300308.2017. doi: 10.1534/g3.117.300308. PMID: 29025917. |
| MT10784 | dpy-18(e364) lis-1(n3334) III/eT1 (III;V). | C. elegans | Heterozygotes are WT and segregate WT, Unc-36 (eT1 homozygotes), and dpy-18 lis-1 homozygotes which are Mel, Unc, Egl, and Dpy. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| qt9 | Allele | substitution | nonsense |
| qt42 | Allele | substitution | nonsense |
| qt1 | Allele | substitution | |
| qt2 | Allele | substitution |