Laboratory Information
| Name | GN View on WormBase |
|---|---|
| Allele designation | pg |
| Head | Miriam B. Goodman |
| Institution | Stanford University, Stanford, CA |
| Address | B-111 Beckman Center 279 Campus Dr Stanford 94305 United States |
| Website | https://med.stanford.edu/goodmanlab.html |
| Gene classes | atat dlst |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| GN112 | pgIs2. | C. elegans | pgIs2 [gcy-8p::TU#813 + gcy-8p::TU#814 + unc-122p::GFP + gcy-8p::mCherry + gcy-8p::GFP + ttx-3p::GFP]. TU#813 and TU#814 are split caspase vectors (Chalfie Lab) subcloned downstream of the gcy-8 promoter. Expression of GFP in coelomocytes and AIY neuron, and mCherry in AFD neuron when caspase activity is lost. Reference: Glauser DA, et al. Genetics. 2011 May;188(1):91-103. |
| GN231 | pgIR6 (X, CB4856>N2) X. | C. elegans | pgIR6 (X, CB4856>N2). CB4856 Chromosome X in N2 background. |
| GN517 | pgEx116. | C. elegans | pgEx116 [unc-70::TSmod + myo3p::mCherry]. Pick animals with red fluorescence in body wall muscle to maintain array. The tension sensor module (TSMod) was inserted into the coding sequence of unc-70. TSmod consists of a donor (mTFP) and acceptor (Venus) fluorophore separated by a flexible linker made of 40 residues from the spider-silk flagelliform, which acts as an entropic nanospring suitable for estimating biologically relevant forces. Reference: Krieg M, et al. Nat Cell Biol. 2014 Mar;16(3):224-33. |
| GN600 | pgIs22 IV; oxIs95. | C. elegans | pgIs22 [unc-70::N-TSmod]. oxIs95 [pdi-2p::unc-70 + myo-2p::GFP]. The tension sensor module control (N-TSMod) was inserted at the N-terminus of unc-70. N-TSmod consists of a donor (mTFP) and acceptor (Venus) fluorophore separated by a flexible linker made of 40 residues from the spider-silk flagelliform, but is placed at the N-terminus of UNC-70 where it is not sensitive to force. pgIs22 was a spontaneous insertion of pgEx157. Reference: Kelley M, et al. Elife. 2015 Mar 23;4. |
| GN67 | pgIR2 (II, CB4856>N2) II. | C. elegans | pgIR2 (II, CB4856>N2). CB4856 Chromosome II in N2 background. |
| GN675 | tba-1(pg77[tba-1::TagRFP-T + loxP]) I; uIs31 III. | C. elegans | uIs31 [mec-17p::GFP] III. pg77[tba-1::TagRFP-T + loxP] is a CRISPR-mediated c-external tag in the endogenous tba-2 locus. Reference: Lockhead D. et al Mol Biol Cell. 2016 Nov 15; 27(23): 3717–3728. |
| GN68 | pgIR3 (III, CB4856>N2) III. | C. elegans | pgIR3 (III, CB4856>N2). CB4856 Chromosome III in N2 background. |
| GN683 | tbb-1(pg79[tbb-1::TagRFP-T + loxP]) uIs31 III. | C. elegans | uIs31 [mec-17p::GFP] III. pg79[TBB-1::TagRFP-T + loxP] is a CRISPR-mediated c-external tag in the endogenous tba-2 locus. Reference: Lockhead D. et al Mol Biol Cell. 2016 Nov 15; 27(23): 3717–3728. |
| GN688 | tba-2(pg81[tba-2::tagRFP-T + loxP]) I; uIs31 III | C. elegans | uIs31 [mec-17p::GFP] III. pg81[TBA-2::tagRFP-T + loxP] is a CRISPR-mediated c-external tag in the endogenous tba-2 locus. Reference: Lockhead D. et al Mol Biol Cell. 2016 Nov 15; 27(23): 3717–3728. |
| GN69 | pgIR4 (IV, CB4856>N2) IV. | C. elegans | pgIR4 (IV, CB4856>N2). CB4856 Chromosome IV in N2 background. |
| GN70 | pgIR5 (V, CB4856>N2) V. | C. elegans | pgIR5 (V, CB4856>N2). CB4856 Chromosome V in N2 background. |
This laboratory hasn't submitted any alleles to the CGC.