Laboratory Information

NameGL View on WormBase
Allele designationrf
HeadGordon J Lithgow
InstitutionBuck Institute, Novato CA, USA
Address Lithgow Lab
8001 redwood blvd
Novato 94945
United States
Website http://www.buckinstitute.org/lithgowlab
Gene classes cid  faah  lsd  nape 

Strains contributed by this laboratory

Strain Genotype Species Description
GL302 cid-1(rf34) III. C. elegans K10D2.3 Slow development. Reduced fertility. Thermotolerant. Resistant to hydroxyurea.
GL347 zcIs13 V. C. elegans zcIs13 [hsp-6p::GFP + lin-15(+)] V. Stable transgenic line with GFP expression mainly in the posterior intestine, observed from L1 to adult. Perturbation of mitochondrial folding environment induces robust GFP expression throughout the intestines. Derived by outcrossing parental strain SJ4100 to N2 six times to remove suspected background mutations causing uneven transgene expression and low penetrance of sterility.
PHX1826 atp-3(syb1826[atp-3::GFP]) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Endogenous atp-3 locus tagged with GFP by CRISPR/Cas9 engineering. ATP-3::GFP under the atp-3 promoter. ATP-3::GFP expression ubiquitously expressed in mitochondria. Heterozygotes have green pharynx. Homozygotes do not have green pharynx and are sterile. Pick hets to maintain.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
rf34 Transposon insertion insertion