Caenorhabditis Genetics Center (CGC)

Laboratory Information

NameGC View on WormBase
Allele designationna
HeadE. Jane Albert Hubbard
InstitutionNYU Grossman School of Medicine
Address NYU Grossman School of Medicine
522 First Avenue
Joan and Joel Smilow Research Center, 8th Floor, Lab 806
New York 10016
United States
Website http://jhubbardlab.med.nyu.edu/
Gene classes pro  syx 

Strains contributed by this laboratory

Strain Genotype Species Description
GC363 E. coli. Escherichia coli Bacteria. E. coli HT115(DE3) bacterial strain carrying pGC8. pGC8 is a partial cDNA of him-14 (ZK1127.11) cloned into the Timmons and Fire "double T-7 vector" L4440. The source of the cDNA is Yuji Kohara's clone yk240h12. pGC8 was constructed by inserting the 1.65kb KpnI/SacI fragment of the him-14 cDNA (from base pair 1071 to 192 base pairs beyond the stop codon) into the same sites in L4440. HT115(DE3) carrying pGC8 should be selected in the presence of 50 um/ml tetracyline and 100 um/ml ampicillin. Prior to an actual feeding experiment, it can be grown in liquid in the presence of amp alone (no tet) and then seeded onto NGM plates containing amp and 1 mM IPTG. This technique does not work well if the cells are old; therefore, the strain should be seeded onto IPTG-containing plates from a fresh overnight that was grown from a colony on an amp/tet plate. Biosafety Level: BSL-1. For more info see http://www.wormbook.org/wli/wbg17.1p32/
GC463 rpl-11.1(ar228) V/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Sterile non-Unc (homozygous for ar228) and dead eggs. ar228 have severely reduced germline proliferation, which is more severe at 15C than at 25C.
GC565 pro-1(na48)/mIn1 [dpy-10(e128) mIs14] II. C. elegans Heterozygotes are WT with GFP+ (pharynx) and segregate Dpy with GFP+ (pharynx) and slow growing GFP- animals which are generally sterile (with germline tumor at 25C). pro-1(na48) is a weak, recessive, loss-of-function allele that behaves as a stronger loss-of-function at lower temperatures. At 25C, 85% of na48 animals will develop a proximal germline tumorr. Although tumors are less common at lower temperatures, the animals are generally sterile due to low proliferation and somatic gonad defects. na48 animals are slow growing at all temperatures.
GC589 pab-1(ar232)/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are WT and GFP+, and segregate Sterile non-GFP (homozygous ar232) and dead eggs. ar232 have severely reduced germline proliferation.
GC613 eef-1A.1(ar229) III/eT1 (III;V). C. elegans Heterozygotes are WT and segregate WT, Steriles (homozygous ar229), Unc-36 (homozygous eT1), and dead eggs. ar229 have severely reduced germline proliferation.
GC734 pro-2(na27) II. C. elegans At 20C and below, worms have smaller brood size than WT, although the gonad develops properly. At 25C, worms are sterile (either Glp or Pro). The animals grow slower than WT at all temperatures. Maintain at 20C or below.
GC735 pro-3(ar226) V. C. elegans At 20C and below, worms are visibly WT; animals grow somewhat larger than WT. At 25C, segregates WT, Glp and Pro. Maintain at 20C or below.
GC771 him-5(e1490) V; naEx57. C. elegans naEx57 [lag-2p::FLP::let-858 3'UTR + ceh-22p::GFP]. Him. Derived by injection of plasmids pGC158 and pCW2.19. Reference: Voutov, R and Hubbard, EJ. Genetics 180(1):103-19 (2008).
GC773 unc-119(ed3) III; naIs3. C. elegans naIs3 [(pGC133) hsp-16.41::FLP:::let-858 3'UTR) + Cbr-unc-119(+)].
GC817 unc-119(ed3) III; naIs6. C. elegans naIs6 [hsp-16.2p::FLP::let-858 3'UTR + Cbr-unc-119(+) + ceh-22p::GFP)].
GC822 unc-119(ed3) III; naEx75. C. elegans naEx75 [(pGC146) hsp-16.2p::FLP::let-858 3'UTR) + Cbr-unc-119(+) + (pCW2.1) ceh-22p::GFP)]. Array was bombarded but did not integrate.
GC827 unc-119(ed3) III; naIs7. C. elegans naIs7 [hsp-16.2p::FLP::let-858 3'UTR + Cbr-unc-119(+)]. Does not express ceh-22p::GFP, but unc-119 is rescued.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
na48 Allele substitution
na27 Allele substitution