Laboratory Information

NameFGP View on WormBase
Allele designationfgp
HeadRonald T Hay
InstitutionUniversity of Dundee, Dundee, UK
Address School of Life Sciences
Gene Regulation & Expression, Sir James Black Centre
Dow Street
Dundee DD15EH
United Kingdom
Website https://www.pelischlab.co.uk
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
FGP1 unc-119(ed3) III; fgpIs20. C. elegans fgpIs20 [(pFGP79) pie-1p::mCherry::smo-1(GG) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal from fgpIs20. Allows SUMO localization/conjugation to be observed within the germline and embryos. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485.
FGP116 gei-17(fgp1[GFP::FLAG::degron::loxP::gei-17]) I; ieSi65 II; unc-119(ed3) III; ltIs37 IV. C. elegans gei-17(fgp1[GFP::FLAG::degron::loxP::gei-17]) I. ieSi65 [sun-1p::TIR1::sun-1 3’UTR + Cbr-unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Superficially wild-type. FLAG (with degron) inserted into endogenous gei-17 locus. Reference: Pelisch et al. Journal of Cell Science (2019) jcs.232330 doi:10.1242/jcs.232330
FGP14 unc-119(ed3) III; fgpIs35. C. elegans fgpIs35 [smo-1p::6xHis::smo-1(GG)::smo-1 3'UTR + unc-119(+)]. Superficially wild-type. This strain allows identification of SUMO-modified proteins of interest; the 6xHis tag allows for denaturing Ni-NTA purification (ensuring SUMO is not deconjugated by proteases). Reference: Pelisch F & Hay RT. Methods Mol Biol. 2016;1475:233-56.
FGP189 icp-1(syb1147[icp-1::linker::AID::GFP]) I; ieSi65 II; unc-119(ed3) III; ltIs37 IV. C. elegans icp-1(syb1147[icp-1::linker::AID::GFP]) I. ieSi65 [sun-1p::TIR1::sun-1 3’UTR + Cbr-unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Superficially wild-type. GFP (with linker and auxin-inducible degradation signal) inserted into endogenous icp-1 locus. Reference: Pelisch et al. Journal of Cell Science (2019) jcs.232330 doi:10.1242/jcs.232330
FGP2 unc-119(ed3) III; fgpIs21. C. elegans fgpIs21 [(pFGP80) pie-1p::mCherry::smo-1(GA) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal. May be used to follow unconjugated SUMO localization within the germline and embryo. When compared with strain FGP1, the difference in fluorescence signal corresponds to SUMO conjugation in FGP1. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485.
FGP29 gei-17(fgp1[GFP::FLAG::degron::loxP::gei-17]) I; ieSi38 IV. C. elegans gei-17(fgp1[GFP::FLAG::degron::loxP::gei-17]) I. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. Reference: Pelisch et al. Mol Cell. 2017 Jan 5;65(1):66-77.
FGP3 unc-119(ed3) III; fgpIs23. C. elegans fgpIs23 [(pFGP78) pie-1p::GFP::TEV-S-Tag::smo-1(GG) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal. Allows SUMO localization/conjugation to be observed within the germline and embryos. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485.
FGP30 gei-17(fgp1[GFP::FLAG::degron::loxP::gei-17]) I; itIs37 IV. C. elegans gei-17(fgp1[GFP::FLAG::degron::loxP::gei-17]) I. itIs37 [(pAA64) pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Pelisch et al. Mol Cell. 2017 Jan 5;65(1):66-77.
FGP4 unc-119(ed3) III; fgpIs24. C. elegans fgpIs24 [(pFGP77) pie-1p::GFP::TEV-S-Tag::smo-1(GA) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal. When compared with strain FGP3, the difference in fluorescence signal corresponds to SUMO conjugation in FGP3. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485.
FGP7 unc-119(ed3) III; ruIs57; fgpIs20. C. elegans ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. Expression of GFP::tubulin fusion in germline and early embryos. fgpIs20 [(pFGP79) pie-1p::mCherry::smo-1(GG) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal from fgpIs20. Allows SUMO localization/conjugation to be observed within the germline and embryos. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485.
FGP77 bub-1(syb425[bub-1::linker::degron::gfp]) I; ieSi65 II; unc-119(ed3) III; ltIs37 IV. C. elegans bub-1(syb425[bub-1::linker::degron::gfp]) I. ieSi65 [sun-1p::TIR1::sun-1 3’UTR + Cbr-unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Superficially wild-type. GFP (with linker) inserted into endogenous bub-1 locus. Reference: Pelisch et al. Journal of Cell Science (2019) jcs.232330 doi:10.1242/jcs.232330
FGP8 unc-119(ed3) ruIs32 III; fgpIs20. C. elegans ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Expression of GFP::H2B histone fusion in germline. fgpIs20 [(pFGP79) pie-1p::mCherry::smo-1(GG) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal from fgpIs20. Allows SUMO localization/conjugation to be observed within the germline and embryos. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485.
This laboratory hasn't submitted any alleles to the CGC.